SEPARATION AND PURIFICATION TECHNOLOGY
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2023
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IF 8.6
Xuran Liu,Xianli Yang,Jingnan Yang,Yanxin Wu,Dongbo Wang
The quality and quantity of short chain fatty acids (SCFAs) production from anaerobic fermentation of sewage sludge is generally constrained by low organics solubilization and poor substrate-availability. This study provided an environmentally friendly strategy with zero valent iron (ZVI) activating sulfite pretreatment to overcome this limitation and advance the sludge anaerobic fermentation. Experimental results showed that the maximal SCFAs production and acetate proportion promoted by 5.7 and 2.75 times respectively at 500 mg S/L and the ZVI/sulfite ratio of 1.25. Mechanism analysis elucidated that the free radicals of S O 4 - · and ·OH produced from ZVI-sulfite pretreatment accelerated the disintegration of sludge-flocs and decreased the value of α-helix and ratio of α-helix/ (β-sheet + random coil) from 21.64 % and 44.23 % to 17.80 % and 38.67 %, respectively, which provided more easily- and bio- available substance for functional anaerobes. The ZVI-sulfite pretreatment enhanced the ability of functional anaerobes for SCFAs production processes as hydrolysis, acidogenesis and acetogenesis and reduced the SCFAs consumption as methanogenesis, which ultimately promoted SCFAs accumulation. Microbial community illustrated that ZVI-sulfite pretreatment enriched the relative abundance of functional anaerobes related to hydrolysis, acidogenesis and acetogenesis bioprocesses. PICRUSt analysis demonstrated that the pretreatment boosted the functional genes abundance involved in pathway of butyrate and acetate formation. Moreover, the enrichment genes of acetate formation were consistent with the increase of acetate proportion.
研究领域:
环境工程
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微生物学
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生物技术
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污水处理
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产品关联
DOI
高影响因子
ECOTOXICOLOGY
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2024
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IF 2.4
Wang Jie,Huang Hexi,Yang Hailin,Wang Su,Li Mengnan,Zhu Zhengyang,Trumble John T.,Di Ning,Zang Liansheng
Cadmium (Cd), a widely distributed environmental pollutant in agroecosystems, causes negative effects on crops and herbivores through bottom-up processes. The gut microbial community of an insect can play a critical role in response to metal stress. To understand how microbiota affect the stress responses of organisms to heavy metals in agroecosystems, we initially used 16S rRNA sequencing to characterize the larval gut microbiota of Chilo suppressalis , an important agricultural pest, exposed to a diet containing Cd. The species richness, diversity, and composition of the gut microbial community was then analyzed. Results revealed that while the richness (Chao1 and ACE) of gut microbiota in larvae exposed to Cd was not significantly affected, diversity (Shannon and Simpson) was reduced due to changes in species distribution and relative abundance. Overall, the most abundant genus was Enterococcus , while the abundance of the genera Micrococcaceae and Faecalibaculum in the control significantly superior to that in Cd-exposed pests. Phylogenetic investigation of microbial communities by the reconstruction of unobserved states (PICRUSt) showed that the intestinal microorganisms appear to participate in 34 pathways, especially those used in environmental information processing and the metabolism of the organism. This study suggests that the gut microbiota of C. suppressalis are significantly impacted by Cd exposure and highlights the importance of the gut microbiome in host stress responses and negative effects of Cd pollution in agroecosystems.
研究领域:
环境科学
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昆虫学
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微生物学
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生态毒理学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2024
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IF 7.4
Huili Zeng,Jingyi Huang,Minghuo Wu,Minjie Hu,Xiaojing Yang,Hao Zhou,Jingjing Zhan,Xuwang Zhang
As a representative polycyclic aromatic hydrocarbon (PAH), pyrene poses a significant threat to the environment and public health due to its persistence and potential toxicity. Extensive research has been conducted on biodegradation of pyrene, highlighting the crucial need to unravel its degradation mechanisms. Coastal and marine sediments are promising sources for discovering microbial candidates capable of degrading pyrene. In this study, three bacterial consortia, designated as BS, YS, and SS, were enriched from sediments collected from the Bohai Sea, Yellow Sea, and Liao River estuarine wetland, respectively. The bacterial consortia BS, YS, and SS demonstrated high pyrene degradation efficiencies, achieving 86.61 %, 80.39 %, and 75.70 % degradation at the concentration of 50 mg/L within 8 days, respectively. High-throughput sequencing of 16S rRNA gene amplicons revealed Pandoraea , Delftia , and Pseudomonas as the dominant populations in bacterial consortia BS, YS, and SS, respectively, with some species potentially being novel pyrene degraders. Further analysis included identifying pyrene degradation metabolites by LC-Q-TOF-MS (high-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry) and predicting functional genes through PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved Stats). Based on these results, the metabolic pathways for pyrene degradation by the bacterial consortia were proposed, which predominantly involved the dioxygenation pathway. Overall, these findings should offer valuable bacterial resources for facilitating the bioremediation of PAH-contaminated environments.
研究领域:
环境微生物学
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生物修复
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海洋生态学
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分子生物学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2025
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IF 9.8
Zi Ye,Leilei Yu,Chuan Zhang,Xiaojing Wei,Jianxin Zhao,Wei Chen,Qixiao Zhai,Fengwei Tian
Volvaria volvacea polysaccharides (VVP) possess diverse bioactivities with promising applications in biomedicine and functional foods. This study investigated the metabolic fate of VVP in human gut microbiota and uncovered the pivotal role of Bacteroides thetaiotaomicron using in vitro fecal fermentation models. VVP selectively promoted B. thetaiotaomicron proliferation, establishing it as a central hub in microbial interaction networks. Metabolomic profiling revealed significant increases in short-chain fatty acids (SCFAs) and adenosine during VVP fermentation. KEGG pathway enrichment demonstrated activation of starch and sucrose metabolism and histidine biosynthesis, consistent with PICRUSt functional predictions. Network correlation and sole‑carbon source assays confirmed B. thetaiotaomicron as a key VVP degrader. These findings establish B. thetaiotaomicron as a dual-function mediator that orchestrates VVP bioconversion into bioactive metabolites and reshapes gut microenvironment. This mechanistic framework supports the development of VVP-based, microbiota-targeted interventions to enhance gut health.
研究领域:
微生物学
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糖生物学
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代谢组学
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功能性食品
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生物医学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Cleaner Production
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2021
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IF 9.297
Libing Chu,Jianlong Wang,Chuanhong Chen,Shijun He,László Wojnárovits,Erzsébet Takács
The advanced treatment of secondary effluent from an antibiotic wastewater treatment plant was investigated using ionizing radiation alone and combined with peroxymonosulfate (PMS)/H 2 O 2 oxidation . In the effluent, 3 antibiotics, including erythromycin (ERY), tetracycline (TC), sulfamethoxazole (SMX), and 7 groups of antimicrobial resistance genes (ARGs) and 12 bacterial classes were detected. Results showed that the content of antibiotics and ARGs could be reduced greatly by radiation. The removal of ERY, TC and SMX reached 87%, 80% and 48% respectively, while the abatement of ARGs, including erm B, erm T, erm F, maA/mel , sul 2, bla OXA , flo R and mef A reached 63–91%. The COD removal was only 16.8% at 25 kGy. The enhancement with PMS addition was better than with H 2 O 2 addition during radiation. With 10 mM PMS addition, the removal efficiency of antibiotics and COD increased to 70–98% and 57.6%, compared to 55–97% and 45.1% with 10 mM of H 2 O 2 addition at 25 kGy. The PICRUSt analysis suggested that radiation at a dose of higher than 25 kGy could stimulate the bacterial activity for metabolism of amino acid, carbohydrate and energy, etc, but damage the genes coding for cellular and genetic information processing. The major ecological risk is derived from ERY (high level) and TC (medium level) in the effluent. The ecological risk of ERY and TC could be declined to medium level and low level, respectively after radiation with 10 mM PMS/H 2 O 2 addition at 25 kGy. However, the operating cost of EB/PMS processes was more than twice higher than that of EB/H 2 O 2 processes. Based on the tech-economic evaluation, ionizing radiation combined with H 2 O 2 was a promising technology for the advanced treatment of pharmaceutical wastewater.
研究领域:
环境工程
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污水处理
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辐射化学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Yu-Qi Li,Bai-Hang Zhao,Yu-Qing Zhang,Hai-Shan Yang,Bing-Lin Zhang
Multiple nanoparticles presented in wastewater treatment plants can produce comprehensive ecotoxicity. In this study, copper oxide nanoparticles (nCuO) and zinc oxide nanoparticles (nZnO) were investigated for their co-toxicity to aerobic granular sludge (AGS) systems. The combined stress of nCuO and nZnO performed significant antagonistic effects on nitrogen removal both under acute and chronic stress. Under chronic stress, nCuO and nZnO co-existence performed significant antagonistic effects on cell membrane, adenosine triphosphate (ATP) activity and microbial community structure. PICRUSt analysis indicated that nCuO (15 mg/L) co-existence alleviated nZnO (10 mg/L) inhibition on energy production and membrane transport metabolic pathways. In addition, nCuO addition to single nZnO weakened the toxicity of nZnO to nitrogen metabolism, glucose metabolism, and electron transfer genes, contributing to an alleviation in nitrogen removal inhibition. As compared with individual stress, combined stress decreased Cu(II) release from nCuO, but increased Zn(II) release from nZnO. The combination of nCuO and nZnO increased zinc resistance gene amplification and czcC gene proportion, and promoted Zn(II) efferent from cells, also with AGS resistance.
研究领域:
环境工程
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微生物学
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废水处理
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纳米技术
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生态毒理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）