Lan Zhao,Xinran Yang,Peijie Ye,Zhihai Lan,Jiale Cheng,Li Li,Jia Wu,Wei Xu,Huibin Chen
Qingke β-glucan (QBG), a gastrointestinal indigestible grain polysaccharide, is used as prebiotic and delivery carrier to mediate gut microbiota, while the mechanism of QBG of different MWs degraded by the gut microbiota remain uncertain. In this study, we investigated the impacts of QBG with different MWs on gut microbiota, SCFAs, and the degrading degree of polysaccharides in vitro fermentation. QBG groups significantly increased the total SCFAs, which is consistent with the results predicted by PICRUSt2 based on the KEGG pathway. The 27 functional genes associated with SCFAs metabolism were identified. Acetate and propionate synthesis was primarily mediated by the pta, ackA , and ACSS genes, whereas butyrate production mediated by the ptb, buk , and atoA genes. The low-MW QBG produced more SCFAs than the high-MW QBG after fermentation, suggesting that polysaccharide MW influences its biological functionality. These findings support polysaccharide-based gut microbiota-targeting drug and food development.
Ya Gao,Ying Xiao,Ling Yu,Qingjiu Tang,Lin Ye,Jinyan Wang,Yipeng Wang,Fangfang Zhang,Jianjin Zhou
Polyonatum cyrtonema polysaccharides (PCPs) have been illustrated to have pharmacological and biological activity. However, the impact of PCPs on gut microorganisms and their metabolic pathways has been scarcely addressed. In order to investigate the antidigestion potential of PCPs, an in vitro simulation of digestion was developed. Moreover, an in vitro fermentation model was used to explore the influence of PCPs on the microbial community. The findings revealed that the concentration of the remaining carbohydrate and reducing end of the polysaccharide chain grew slightly, while the molecular weight was unaltered after being digested. Notably, the gut microbiota degraded the PCPs during the subsequent fermentation, resulting in the production of short-chain fatty acids and a significant decrease in the pH level ( p < 0.05). Compared with the control group, the result of 16S rRNA sequencing revealed that PCPs could affect the diversity of the community and the composition of the gut microbes significantly ( p < 0.05), having similar regulatory effects to inulin on the structure of the microbial community. The PCPs group notably promoted the relative abundance of acetic-acid bacteria, such as Bifidobacterium . In addition, PICRUSt2 analysis demonstrated that PCPs mainly enhanced phenotypic KEGG pathways such as amino acid metabolism and carbohydrate metabolism. Overall, PCPs may have prebiotic properties that support human health by controlling the make-up and metabolic activity of intestinal flora.
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