CLINICA CHIMICA ACTA
·
2023
·
IF 5
Ying Xu,Chenguang Hu,Jiayu liu,Haoran Guo,Jingkai Gao,Chi Wang,Chengbin Wang
Background and Aims Synovial fluid lactoferrin (LTF) and S100 calcium-binding protein A8 (S100A8) have been considered as potential biomarkers for the diagnosis of periprosthetic joint infection (PJI) through our previous research. However, the detection methods of these two proteins are still immature, so a rapid, accurate and cost-effective testing method is warranted. Materials and Methods We developed chemiluminescent immunoassays (CLIA) for the automated detection of synovial fluid LTF and S100A8 and assessed the analytical performance for these two methods. In addition, we recruited 86 patients who were suspected of PJI after total joint replacement (TJA) and examined their synovial fluid using CLIA to explore the clinical application value of these methods and the diagnostic efficiency of synovial fluid LTF and S100A8 for PJI. Results Our established CLIA methods have a wide linear range of 20–10,000 ng/mL for LTF detection system and 5–5000 ng/mL for S100A8 detection system. Performance parameters such as precision, specificity, and recovery rate can meet the industry standards. Then, the established methods were used to detect LTF and S100A8 in synovial fluid samples, which showed excellent diagnostic efficiency for PJI, and the areas under ROC curve (AUC) were 0.954 (95 % CI : 0.909–0.999) and 0.958 (95 % CI : 0.918–0.997), respectively. Conclusion Our established CLIA methods have the advantages of automation, high throughput, low price, and is expected to be widely popularized in clinical applications. Synovial fluid LTF and S100A8 detected through CLIA had efficient diagnostic potentiality for predicting and diagnosing PJI.
研究领域:
临床诊断
·
骨科
·
免疫学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Zheng Liu,Jimei Chi,Zewei Lian,Yaqi Yang,Lijun Cheng,Xiaoxue Lin,Xuwei He,Xiaojian Ji,Chenxuan Yang,Yunhao Tang,Minzhi Yang,Zhisen Gao,Wang Gu,Haoming An,Qingda Wei,Yanlin Song,Meng Su,Rui Li,Wei Chai
Periprosthetic joint infection (PJI) is a catastrophic complication after joint arthroplasty. A key step to treat PJI is accurate diagnosis. However, according to the existing guidelines, no biomarker has been used to diagnose PJI alone and autoimmune diseases such as rheumatoid arthritis (RA) often interfere with the diagnosis. Patients with PJI often still face issues such as misdiagnosis and delayed treatment after a comprehensive scoring diagnosis. Thus, a rapid quantitative detection method based on excellent biomarker is needed. Here, an all-printed photonic crystal (PC) biochip is reported. Through the fluorescence enhancement effect of the photonic crystals and the design of the hydrophilic and hydrophobic patterning structure, trace detection of lactoferrin (LTF) in a drop of viscous synovial fluid can be achieved within 10 min by the biochip. The detection limit is as low as 4.6 ng/mL. For more intuitive results, portable testing equipment is developed with indicator light displays of three colors (green = NPJI, yellow = RA, and red = PJI) to indicate the diagnosis depending on the biochip’s fluorescence intensity. Furthermore, 79 clinical synovial fluid samples are tested with PC biochips, and the PC results are in good agreement with the ELISA results. Due to the microdroplet reaction on PCs, biochips are suitable for the detection of the selected markers in the synovial fluid. The use of the three-color portable testing equipment avoids the need for professional testing devices. Overall, this strategy provides a new paradigm for the early and differential diagnoses of PJI.
研究领域:
生物医学工程
·
临床诊断
·
生物标志物检测
·
即时检验
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 45
·10428-10438
·
IF 5.344
Yue Ren,Fei-Yu Wang,Zi-Jian Chen,Ri-Tong Lan,Ren-Huan Huang,Wan-Qun Fu,Rizwan M. Gul,Jing Wang,Jia-Zhuang Xu,Zhong-Ming Li
Periprosthetic joint infection (PJI) is one of the main causes for the failure of joint arthroplasty. In view of the limited clinical effect of oral/injectable antibiotics and the drug resistance problem, there is a pressing need to develop antibacterial implants with therapeutic antimicrobial properties. In this work, we prepared a highly antibacterial ultrahigh molecular weight polyethylene (UHMWPE) implant by incorporating tea polyphenols. The presence of tea polyphenols not only improved the oxidation stability of irradiated UHMWPE, but also gave it the desirable antibacterial property. The potent antibacterial activity was attributed to the tea polyphenols that produced excess intracellular reactive oxygen species and destroyed the bacterial membrane structure. The tea polyphenol-blended UHMWPE had no biological toxicity to human adipose-derived stem cells and effectively reduced bacteria-induced inflammation in vivo. These results indicate that tea polyphenol-blended UHMWPE is promising for joint replacement prostheses with multifunctionality to meet patient satisfaction.
研究领域:
生物材料
·
生物医学工程
·
骨科
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）