APPLIED CATALYSIS A-GENERAL
·
2022
·
IF 5.723
Weilu Wang,Xiangke Zeng,Shan He,Haidong Zhang,Guangming Jiang,Yang He,Yunqi Liu,Fan Dong,Xianming Zhang,Steven L. Suib
A series of Fe-doped alumino-borate catalysts (18 % Fe-PKU-1) with a range of crystalline qualities has been obtained by varying synthesis conditions. Based on Angle-resolved X-ray Photoelectron Spectroscopy, pyridine adsorption infrared spectroscopy, and potentiometric titration, crystalline quality is correlated as volcano trends with the surface composition and electronic structure of Fe-PKU-1, especially for the boron species. Through the systematic change of synthesized conditions, the catalyst with the appropriate crystalline quality shows the highest surface compositions of boron and oxygen vacancies. This leads to strong surface Lewis acidity and results in the high catalytic performance of glycerin ketalization, 97 % conversion and 99 % selectivity in DL-1,2-Isopropylideneglycerol (Solketal).
研究领域:
催化
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材料科学
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化学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
·
2023
·
IF 4.4
Shuhua Zhang,Yao Zheng,Zhengyan Wu,Yuanyuan Zhang,Xiaohui Liu,Zhigang Luo,Hongjuan Li,Hongbo Li,Jinghua Yu
In this study, we developed a two-step enzymatic hydrolysis method followed by activated carbon adsorption to remove phenylalanine (Phe) from whey protein. The results showed that the free rate of Phe after sequential enzymatic hydrolysis of trypsin and flavorzyme was 90.8%. After adsorption by activated carbon No. 1, the removal efficiency of Phe from the hydrolysates reached 89.79% at 2.19 mg/g protein equivalent. Therefore, low Phe protein hydrolysates (LPHs) can be used to prepare low Phe food for phenylketonuria patients who cannot metabolise Phe properly by themselves and need to maintain a low Phe diet for life.
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DOI
高影响因子
Advanced Science
·
2024
·Vol. 11
·期 15
·2305701
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IF 14.3
Haiyang Su,Huiwen Zhang,Jiao Wu,Lin Huang,Mengji Zhang,Wei Xu,Jing Cao,Wanshan Liu,Ning Liu,Hongwei Jiang,Xuefan Gu,Kun Qian
Phenylketonuria (PKU) is the most common inherited metabolic disease in humans. Clinical screening of newborn heel blood samples for PKU is costly and time-consuming because it requires multiple procedures, like isotope labeling and derivatization, and PKU subtype identification requires an additional urine sample. Delayed diagnosis of PKU, or subtype identification can result in mental disability. Here, plasmonic silver nanoshells are used for laser desorption/ionization mass spectrometry (MS) detection of PKU with label-free assay by recognizing metabolic profile in dried blood spot (DBS) samples. A total of 1100 subjects are recruited and each DBS sample can be processed in seconds. This platform achieves PKU screening with a sensitivity of 0.985 and specificity of 0.995, which is comparable to existing clinical liquid chromatography MS (LC-MS) methods. This method can process 360 samples per hour, compared with the LC-MS method which processes only 30 samples per hour. Moreover, this assay enables precise identification of PKU subtypes without the need for a urine sample. It is demonstrated that this platform enables high-performance and fast, low-cost PKU screening and subtype identification. This approach might be suitable for the detection of other clinically relevant biomarkers in blood or other clinical samples.
研究领域:
医学诊断
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质谱分析
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代谢组学
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临床化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
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IF 5.162
Baoting Sun,Zichen Wang,Xiaoyi Wang,Mengxia Qiu,Zhijin Zhang,Ziyuan Wang,Jiandong Cui,Shiru Jia
The Phenylketonuria (PKU) is an inborn defect of phenylalanine (Phe) metabolism, in which Phe accumulated in the blood causing alterations at the central nervous system. Therefore, the detection of PKU is very important for the early diagnosis of PKU patients. However, existing tests for PKU are time-consuming and require high-resource laboratories. In this study, a novel paper-based biosensor based on phenylalnine ammonia lyase (PAL) hybrid nanoflowers was constructed that provides a semi-quantitative output of the concentration of Phe from urine samples. [email protected] 3 (PO 4 ) 2 hybrid nanoflowers ( [email protected] ) were first prepared using PAL and Ca 2+ . Synthesis conditions of the [email protected] on the formation of the [email protected] were optimized. The [email protected] exhibited 90% activity recovery under optimal condition. Compared with free PAL, the [email protected] displayed good storage stability and increased tolerance to proteolysis. After five consecutive operating cycles, the [email protected] still retained 73% of its initial activity, indicating excellent reusability. Furthermore, the paper-based biosensor was able to detect Phe concentration in urine samples, and exhibited good linearity to the Phe concentrations in the range from 60 to 2400 μM and the response time was only about 10 min. Therefore, the paper-based biosensor can be a promising candidate as a biosensor for the detection of PKU.
研究领域:
生物技术
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医学诊断
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纳米技术
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生物化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）