JOURNAL OF MATERIALS RESEARCH
·
2022
·Vol. 38
·期 2
·368-379
·
IF 2.909
Gao Jia,Zhang Yuan,Feng Wenjing,Zhang Ying,Cheng Cui,Han Xiao
Surface amino-rich GQDs-ε-PL is prepared by changing the addition amount of ε-PL. Then, GQDs-ε-PL@4-arm PEG-BA/QCS hydrogels (GQDs-ε-PL@Gel) are synthesized through dynamic imine bonds cross-linking. The sol can transform quickly to gel and the gelation time can be controlled by adjusting the specific gravity of the input raw materials to water. FT-IR and thermogravimetric analyses indicate the successful synthesis of GQDs-ε-PL and GQDs-ε-PL@Gel. The microstructure observation reveals that GQDs-ε-PL has a sheet-like structure with an average size of 65 nm, while GQDs-ε-PL@Gel has a porous network structure. Both GQDs-ε-PL and GQDs-ε-PL@Gel have good fluorescence stability, photothermal and cytocompatibility, and display better antibacterial effect against Escherichia coli , Staphylococcus aureus and Pseudomonas aeruginosa through chemical and photothermal synergistic sterilization. More importantly, GQDs-ε-PL@Gel can repeatedly self-heal after being damaged, which is more beneficial to provide an effective wound closure environment for wounds and to be used as wound dressings.
Graphical abstract
研究领域:
材料科学
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生物医学工程
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纳米技术
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抗菌研究
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DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Kaifeng Yang,Fangming Han,Yafeng Jin,Xiaobo Li
Herein, a stable and ultra-sensitive rutin electrochemical sensor was successfully developed. This sensor based on glassy carbon electrode (GCE) modified with C-GCS@ZIF-F/PL nanocomposite, which was made of thermally carbonized glucose (GCS) doped with flower-like ZIF (ZIF-F) and pencil lead (PL). The electrochemical response of rutin was considerably significant at C-GCS@ZIF-F/PL/GCE, demonstrating favorable conductivity and electrocatalytic properties for detection of rutin. Under optimal conditions, the linear range is 0.1–100 μM, with a low detection limit (LOD) of 0.0054 μM. It also exhibits excellent stability, reproducibility, as well as selectivity over common interfering ions such as Na + , uric acid, quercetin and riboflavin, etc. Meanwhile, the practical utility of developed sensor was evaluated in food samples including honey, orange, and buckwheat tea, achieving satisfactory recovery rates ranging from 98.2% to 101.7%. This paper introduces a novel technique for the detection of rutin in foods.
研究领域:
分析化学
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食品科学
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电化学传感器
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DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2020
·Vol. 167
·期 14
·147520
·
IF 3.721
Xinyi Wang,Liubing Kong,Shuqi Zhou,Tao Liang,Hao Wan,Ping Wang
Photoluminescence (PL) sensors based on quantum dots (QDs) are difficult to achieve exact detection in sample waters. In this paper, polyvinyl alcohol (PVA) based CdTe/ZnS/CdS QDs and fluorescein isothiocyanate (FITC) fluorophores were used to build a ratiometric PL sensor. Combining with the homemade smartphone-based PL E-eye, the Cd2+ exact detection can be achieved. PVA was used to connect QDs and FITC fluorophores without unnecessary ligand exchange and purification. For QDs fluorophore, ethylenediaminetetraaceticacid (EDTA) was used on the surface to induce the specifically Cd2+ recognition site. For FITC fluorophore, the PL remains unchanged in this experiment. Thus, the ratio of two fluorophores can be used to provide a built-in correction. The PL changes with the increase of Cd2+ concentrations could be displayed as a visual color change on the smartphone. Further quantitative analysis could be carried out by the RGB value of the picture through the App in less than 1 min The ratio of R/G is linear to Cd2+ concentration in the range of 1–2000 μg l−1 with a low LOD of 0.057 μg l−1 (S/N). Compared with traditional analysis methods, the PL ratiometric method with PL E-eye is portable, rapid, visible and highly selective especially in discriminate Cd2+ from Zn2+.
研究领域:
环境科学
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分析化学
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纳米技术
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光学传感
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DOI
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2019
·Vol. 34
·期 3
·e4748
·
IF 1.748
Chu Liu,Xiangyong Yu,Zhaorui Meng,Ning Zhao,Fujuan Bai,Longshan Zhao
A simple and efficient method involving microwave-assisted extraction (MAE) combined with GC–MS was established to determine 1,3-di- tert -butylbenzene (DBB), 2,4-di- tert -butylphenol (DBP), 3,5-di- tert -butyl-4-hydroxybenzaldehyde (DBHBA) for evaluating the compatibility of antimicrobial peptide PL-5 spray with drug-packaging materials. In this study, the antimicrobial peptide PL-5 spray was purified using a Welchrom C18 column, and the high-density polyethylene spray bottle with liquid collection tube was first mixed with absolute ethanol, which would be subjected to MAE for further measurement by GC–MS. Various experimental parameters were systematically optimized, and good linearities were obtained within the range of 0.05–1.00 μg/mL for DBB and DBHBA and 0.50–10.00 μg/mL for DBP, with limits of detection (LODs) of 0.99 ng/mL (DBB), 16.34 ng/mL (DBP), and 1.64 ng/mL (DBHBA). Satisfactory recoveries that ranged from 96.0% to 107.2% were acquired, and the relative standard deviation was ≤ 4.7%. The results showed that the maximum daily usage of DBB, DBP, and DBHBA was 9.859, 163.445, and 16.399 ng, respectively, which was far below the corresponding permitted daily exposure values according to the safety assessment, indicating that the migration of analytes did not bring any potential safety risk. The compatibility between the drug and the packaging materials was favorable.
研究领域:
分析化学
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药物分析
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食品安全
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2018
·Vol. 10
·期 1
·277-288
·
IF 3.289
Wanxiu Cao,Chengcheng Wang,Yaoxian Chin,Xin Chen,Yuan Gao,Shihan Yuan,Changhu Xue,Yuming Wang,Qingjuan Tang
Stress exposure can increase the appearance of intestinal dysfunction. DHA and EPA have been shown to possess significant anti-inflammatory and immuno-enhancement bioactivities. The aim of the study was to investigate whether different forms of DHA or EPA would affect intestinal barriers (including intestinal epithelium integrity and immunity responses, gut microbiota and its metabolites) in mice under chronic stress, and might therefore prevent stress induced intestinal dysfunction. Chronic stress caused a series of anomalies in the intestine, including decreased faecal water content, increased pro-inflammatory cytokines (IFN-γ, TNF-α, IL-1β and IL-6), reduced expression levels of ZO-1, occludin and E-cadherin, and aberrant microbiota composition (especially Roseburia spp., Prevotella spp., bifidobacteria and lactobacilli) and its metabolites, mainly LPS, acetic acid, propionic acid and butyric acid. Our data indicated that both DHA-PL and EPA-PL counteracted these adverse effects effectively. In conclusion, DHA-PL and EPA-PL may effectively protect mice against intestinal dysfunction under chronic stress exposure as potential ingredients for functional food.
研究领域:
营养学
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免疫学
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胃肠病学
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微生物学
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2021
·Vol. 39
·期 8
·1020-1028
·
IF 3.603
Liu Xiao,Guo Jian-Wei,Liu Ya-Dong,Liu Ming,Liu Hui,Han Miao-Miao,Ji Sheng-Xiang
Medical devices-related infections pose a great threat to patients and cause an increased morbidity and mortality. Herein, we prepare an antibacterial composite (TPU- x ) through blending medical grade thermoplastic polyurethane (TPU) and the complex (PL-DOSS) of ε -polylysine ( ε -PL) and docusate sodium (DOSS). >99% reduction of colony forming unit (CFU) can be obtained in TPU- x composite films even at relatively low content of PL-DOSS, e.g. 0.13% for Methicillin resistant S. aureus (MRSA) and 0.5% for E. coli . The excellent antibacterial activity is mainly attributed to the formation of PL-DOSS nanoparticles that are uniformly dispersed in the TPU matrix with a size of ∼100 nm. TPU- x composite films exhibit long-term stability in saline and good biocompatibility, and retain mechanical properties of TPU.
研究领域:
生物医学工程
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材料科学
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微生物学
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产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2019
·
IF 4.175
Wanying Wu,Mengmeng Lun,Zhifeng Xing,Hanzhao Song,Aihua Yao,Xunsheng Zhou,Zhong Wan,Kun-ming Gu,Sheng Yu,Qiliang Li,Yinzhen Wang,Wei Li,Guannan He,Benli Chu,Qinyu He
Te doped Yttrium-aluminum (Y 2 O 3 –Al 2 O 3 ) system powders were synthesized by solid-state method. The structure and optical properties were investigated by XRD , SEM, XPS and PL. XRD analysis indicated that the samples transform from YAM, YAP to YAG phase as the sintering temperature rises. The samples sintered below 1200 °C have a broad emission centered at 604 nm with a lifetime up to 13.33 μs under room temperature. While in the mix phase of YAP and YAG, a sharp and intense emission peak centered at 715 nm was monitored with a lifetime up to approximate 2.76 ms. Moreover, the band structure and density of states for Te doped YAGwere investigated by first-principle calculations.
研究领域:
材料科学
·
光学
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产品关联
DOI
高影响因子
PLoS One
·
2026
·Vol. 21
·期 6
·e0351679
·
IF 2.8
Wen Lin,Qiong-zhi Shi,Xiang-ru Liao,Yuan Zeng,Xiang-yang Xie,Gang-jian Ji,Yin-ke Li
Burn wound infections are frequently complicated by biofilm-forming and multidrug-resistant pathogens, particularly methicillin-resistant Staphylococcus aureus (MRSA), posing major therapeutic challenges. Antimicrobial peptides (AMPs) such as PL-5 (peceleganan) exhibit broad-spectrum activity but are limited by instability, poor biofilm penetration, and reduced efficacy in complex wound environments. Here, a red-light-responsive, porphyrin-phospholipid (PoP)-containing cationic liposomal system for PL-5, aiming to enhance its antibacterial and antibiofilm performance was developed. Optimized liposomes achieved high encapsulation efficiency (~73%), uniform nanoscale size (~50 nm), narrow polydispersity, and positive surface charge. They demonstrated good storage stability and controlled peptide release under red-light irradiation (635 nm). In vitro , red-light activation significantly enhanced antimicrobial activity against MRSA and methicillin-susceptible S. aureus (MSSA), reducing minimum inhibitory concentration (MIC) values fourfold and accelerating bactericidal kinetics compared with free PL-5 and non-irradiated liposomes. Additionally, red-light-activated liposomes markedly inhibited biofilm formation. These results indicate that light-responsive liposomal delivery enables spatiotemporally controlled release of PL-5, significantly potentiating its antibacterial and antibiofilm efficacy. This approach offers a promising localized treatment strategy for biofilm-associated burn wound infections and a foundation for future translational studies.
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DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2019
·
IF 2.961
Lian Hu,Chaoqun Ma,Guoqing Chen,Zhuowei Zhu
In this study, four batches of a QD-Ag hybrid in liquid state were prepared by mixing CdS quantum dots (QDs) with dispersions of Ag nanoparticle (NP). The luminescence-related properties of the CdS–Ag hybrid were tuned by adding Ag NPs. For each QD-Ag hybrid, the influence of the Ag/Cd ratio on the evolution of emission was studied systematically. We found that the PL properties of the hybrid depended on the amount of Ag concentration rather than the plasmonic properties of Ag NPs. In the hybrid, Ag doping occurred and caused the PL properties to change. As the number of Ag NPs increased, the band-edge emission decreased monotonically while the PL intensity of the dopant increased first and then decreased. A hypothetical model is proposed to explain the PL change of the QD-Ag hybrid with varying amounts of the Ag component.
研究领域:
材料科学
·
光学
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Feng Qi,Yangyang Li,Tanglei Zhang,Weiyu Dou,Riyue Shu,Zhikan Yao,Jing Wang,Lin Zhang
Segregating airborne particulate matters (PMs, especially PM 1.0 ) with high transmissibility of bacterial and viral pathogens is crucial for ensuring outdoor breathing safety. Electrospun nanofiber membranes (ENMs) have shown great promise for this purpose. However, the application of traditional ENMs is usually plagued by bacterial growth causing severe public health concern. Although some research has been done to overcome this challenge by doping antibacterial materials, the potential negative effects of these materials on the human body cannot be ignored. In this study, the ε-Poly-L-lysine (ε-PL), a green broad-spectrum antibacterial reagent, was grafted onto the surface of polydopamine (PDA) coated polyacrylonitrile (PAN) ENM via addition of Schiff base and Michael reaction. Experimental results indicated that the introduction of the ε-PL is key to the antibacterial performance of the membranes, and the antibacterial efficacy of E. coli and B. subtilis can reach to 99.86 % and 99.99 % respectively. Even after 7 days, the antibacterial efficiency of membrane remained at a high level. Additionally, the uniform structure of membranes endowed it with a high filtration efficiency of 80.9 % for PM 1.0 particles with a moderate transmembrane pressure drop (TMP) of 250 Pa and the corresponding quality factor is approximately 0.0066 Pa −1 . This study has far-reaching implications for the design of green antibacterial nanofibrous membranes to realize the segregation of harmful PMs.
研究领域:
材料科学
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环境科学
·
生物学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Qing Luo,Chenyue Yan,Ruoteng Ma,Lei Dai,Wenjun Shu,Anum Asghar,Zhigang Jia,Xulong Zhu,Shun Yu
The development of multifunctional hydrogels, especially hydrogels with antibacterial and antioxidant properties, plays a crucial role in the effective healing of diabetic wounds. This work proposed a novel composite hydrogel with the oxidized gellan gum (O-GG) and hyaluronic acid (HA) as the hydrogel matrix, and the crosslinking was realized via the Ca 2+ . ε-polylysine/MnO 2 nanozymes (EM) offered antioxidant and antibacterial properties to the corresponding composite hydrogels. The results verified that the O-GG/HA@EM hydrogel exhibited excellent cytocompatibility and injectability. The CCK-8 data indicated that the proliferation rate of mouse fibroblasts reached 314.58 % after three days of coculture with the hydrogel. The hydrogel effectively alleviated oxidative stress and hypoxic microenvironments in wounds, inhibited bacterial infection, and promoted angiogenesis, thereby significantly accelerating the healing process of diabetic wounds. Data from a mouse diabetic wound model revealed that the wound closure rate in the sample group reached 92.4 % after 14 days. This composite hydrogel provides a novel and effective solution for the treatment of chronic diabetic wounds.
研究领域:
生物材料学
·
医学
·
纳米医学
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产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2024
·Vol. 108
·期 1
·1-13
·
IF 3.9
Wu Zongzhan,Dou Wenyu,Yang Xiaolin,Niu Tengfei,Han Zhuzhen,Yang Li,Wang Rufeng,Wang Zhengtao
Abstract Oleanane-type ginsenosides are a class of compounds with remarkable pharmacological activities. However, the lack of effective preparation methods for specific rare ginsenosides has hindered the exploration of their pharmacological properties. In this study, a novel glycoside hydrolase Pl GH3 was cloned from Paenibacillus lactis 154 and heterologous expressed in Escherichia coli . Sequence analysis revealed that Pl GH3 consists of 749 amino acids with a molecular weight of 89.5 kDa, exhibiting the characteristic features of the glycoside hydrolase 3 family. The enzymatic characterization results of Pl GH3 showed that the optimal reaction pH and temperature was 8 and 50 °C by using p -nitrophenyl- β - d -glucopyranoside as a substrate, respectively. The K m and k cat values towards ginsenoside Ro were 79.59 ± 3.42 µM and 18.52 s −1 , respectively. Pl GH3 exhibits a highly specific activity on hydrolyzing the 28- O - β - d -glucopyranosyl ester bond of oleanane-type saponins. The mechanism of hydrolysis specificity was then presumably elucidated through molecular docking. Eventually, four kinds of rare oleanane-type ginsenosides (calenduloside E, pseudoginsenoside RP1, zingibroside R1, and tarasaponin VI) were successfully prepared by biotransforming total saponins extracted from Panax japonicus . This study contributes to understanding the mechanism of enzymatic hydrolysis of the GH3 family and provides a practical route for the preparation of rare oleanane-type ginsenosides through biotransformation. Key points • The glucose at C-28 in oleanane-type saponins can be directionally hydrolyzed. • Mechanisms to interpret PlGH3 substrate specificity by molecular docking. • Case of preparation of low-sugar alternative saponins by directed hydrolysis.
研究领域:
生物化学
·
药理学
·
微生物学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）