JOURNAL OF FOOD ENGINEERING
·
2026
·
IF 5.8
Changhao Liu,Zhicen Liu,Yunfan Tong,Ming Chen,Yue Zhang
Phosphatidylserine (PS) is a vital phospholipid with broad applications in food, cosmetic, and pharmaceutical industries. To overcome the limitations of free phospholipase D (PLD), such as high cost, poor stability, and non-reusability, we developed a novel bilayer immobilization strategy using amino-functionalized magnetic nanoparticles. Magnetic Fe 3 O 4 nanoparticles were sequentially modified with tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES), and genipin (Gnp) was employed as a biocompatible crosslinker to immobilize PLD via combined adsorption and covalent binding. Structural and functional characterization via SEM, FTIR, XRD, and TGA confirmed successful carrier modification and stable enzyme attachment. The optimized immobilized enzyme (Fe 3 O 4 @TA-APTES-Gnp-PLD) exhibited significantly enhanced catalytic performance. Under optimized reaction conditions, it achieved a PS conversion rate 11.27-fold higher than that of free PLD in a Pickering emulsion system, and 7.7-fold higher in a conventional two-phase system. The immobilized PLD also demonstrated excellent operational stability, retaining over 77 % of its initial activity after six consecutive reaction cycles, and 73 % after 12 days of storage at 4 °C. This work presents a sustainable, efficient, and reusable biocatalytic platform for PS synthesis, with strong potential for industrial-scale phospholipid production.
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产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
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2018
·Vol. 94
·期 4
·1220-1229
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IF 2.587
Weide Xiong,Xianhai Zeng,Shih-Hsin Ho,Xueping Ling,Liang Shen,Chuanyi Yao,Yinghua Lu
BACKGROUND Phospholipase D (PLD) has great potential in the pharmaceutical and food industries for manufacturing bioactive phospholipids. Overproduction of PLD in Escherichia coli is difficult due to its high cytotoxicity which causes plasmid instability, short-term PLD synthesis, cell lysis and PLD leakage. To address this issue, integrated optimization of PLD production in upstream and downstream processing was performed. RESULTS Effective strategies in upstream processing include the use of plasmids containing the pSC101 par region in recA − E. coli , the use of moderately strong and tightly regulated promoter P BAD , optimization of codon usage and amino acid supplementation, maintaining the best cellular state by supplementing nutrition, and saturation induction at low temperature. Using these strategies, a large amount of PLD (1.2 × 10 5 U L −1 , 81.5 mg L −1 ) was obtained in the batch culture and PLD synthesis lasted 9 h. Additionally, cell lysis did not occur –99% of the recombinant PLD remained in the periplasm. Ultimately, the PLD was purified 327-fold by a four-step downstream process comprising extraction with 10 mmol L −1 Ethylenediaminetetraacetic acid and 0.05% (w/v) sodium deoxycholate, ammonium sulfate precipitation, anion exchange and Ni 2+ -affinity chromatography. CONCLUSION This study provides the basis for large-scale production of PLD in E. coli . © 2018 Society of Chemical Industry
研究领域:
生物技术
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微生物学
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制药业
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食品工业
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DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Ben Niu,Yingchang Fei,Ruiling Liu,Hangjun Chen,Xiangjun Fang,Weijie Wu,Honglei Mu,Haiyan Gao
The effect of oxyresveratrol on postharvest quality and membrane lipid metabolism of shiitake mushroom was investigated. The result exhibited that oxyresveratrol retarded browning, maintained firmness and alleviated occurrence of decay of shiitake mushroom. The oxidation and hydrolysis of membrane phospholipids were suppressed by oxyresveratrol treatment, which was associated with reduced LOX and PLD activities and increased SOD and CAT activities. The membrane lipidomics of shiitake mushroom was determined by LC-MS. 385 lipid species and 13 fatty acids in membrane lipids were identified by multiple reaction monitoring method. Compared with control group, the phospholipic acid and lysophospholipid reduced by 29.24% and 21.29% in oxyresveratrol-treated group, respectively, which alleviated hydrolysis of phospholipid. Meanwhile, oxyresveratrol maintained the unsaturation of fatty acids and alleviated oxidation of phospholipid. These results demonstrated that oxyresveratrol could play a dual role of inhibiting the oxidation and hydrolysis of phospholipids to mitigate cellular damage of shiitake mushroom.
研究领域:
食品科学
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采后生理学
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脂质组学
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植物生物化学
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DOI
PubMed
高影响因子
Materials
·
2021
·Vol. 14
·期 21
·6716
·
IF 3.623
Shiyu Ma,Yan Lu,Bo Wang,Jinkai Li
White LEDs were encapsulated using Cs4PbBr6quantum dots and Gd2O3:Eu red phosphor as lamp powder. Under the excitation of a GaN chip, the color coordinates of the W-LED were (0.33, 0.34), and the color temperature was 5752 K, which is close to the color coordinate and color temperature range of standard sunlight. The electric current stability was excellent with an increase in the electric current, voltage, and luminescence intensity of the quantum dots and phosphors by more than 10 times. However, the stability of the quantum dots was slightly insufficient over long working periods. The photocatalytic devices were constructed using TiO2, CsPbBr3, and NiO as an electron transport layer, light absorption layer, and catalyst, respectively. The Cs–Pb–Br-based perovskite quantum dot photocatalytic devices were constructed using a two-step spin coating method, one-step spin coating method, and full PLD technology. In order to improve the water stability of the device, a hydrophobic carbon paste and carbon film were selected as the hole transport layer. The TiO2layer and perovskite layer with different thicknesses and film forming qualities were obtained by changing the spin coating speed. The influence of the spin coating speed on the device’s performance was explored through SEM and a J–V curve to find the best spin coating process. The device constructed by the two-step spin coating method had a higher current density but no obvious increase in the current density under light, while the other two methods could obtain a more obvious light response, but the current density was very low.
研究领域:
材料科学
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光电
·
光催化
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DOI
高影响因子
危险品化学品经营许可证（带存储）