FIBERS AND POLYMERS
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2022
·Vol. 23
·期 5
·1225-1234
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IF 2.347
Li Siqi,Yin Jing,Xu Lan
As biomaterials with excellent biocompatibility, biodegradation and low toxicity, poly(ε-caprolactone) (PCL) and poly(lactic-co-glycolic acid) (PLGA) are extensively applied as wound dressing and surgical suture in biomedical field. In this paper, an oblique section free surface electrospinning (OSFSE) apparatus was utilized to prepare high-quality and high-output ZnO/PLGA/PCL nanofiber membranes (NFMs) for antibacterial materials, which could significantly increase the production of ZnO/PLGA/PCL NFMs from 0.01–0.1 g/h of traditional electrospinning to 30.84 g/h of OSFSE. The influences of the weight proportion of PLGA and PCL on the viscosity and conductivity of electrospinning solutions along with the yield, morphology and wettability of PLGA/PCL NFMs were researched, and the optimum weight ratio of 6:4 was determined. Then the effects of ZnO contents on the electrospinning solution properties as well as the yield, morphology, structure, wettability, mechanical property and antibacterial performance of ZnO/PLGA/PCL NFMs with the optimal ratio of PLGA and PCL were studied. The results illustrated that additive nano-ZnO could improve markedly the antibacterial effect of NFMs, and the NFMs with 3 wt% nano-ZnO had excellent antibacterial effect on Escherichia coli (95.3 %) and Staphylococcus aureus (95.7 %).
研究领域:
生物医学工程
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材料科学
·
微生物学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Xinqi Huang,Jianghuiwen Lu,Yumei An,Mingyuan Xu,Xueshi Chen,Chao Liu,Xuefeng Zhou,Haiyan Shan,Yunzhu Qian,Mingyang Zhang
Electrospun nanofibers have a number of qualities that make them a suitable choice for skin wound healing. Lysophosphatidic acid (LPA) stimulates the keratinocytes and fibroblasts to proliferate, differentiate, and migrate and enhances skin wound healing. Here, we developed the electrospun scaffolds contained in polycaprolactone (PCL) and polylactic-co-glycolic acid (PLGA). The scaffolds loaded with LPA nanoparticles retained a porous nanofiber structure and showed better physicochemical properties and biocompatibility. The scaffold continuously releases LPA to quickly initiate cell signaling and maintain long-term anti-inflammatory activity. In this study, we found that PP scaffold with LPA reduces the disordered collagen deposition and the thickness of the newborn epidermis, improves skin healing, and reduces scar formation. Explaining the mechanism of LPA mineralized tissue regeneration in skin wound healing, LPA inhibited the pyroptosis of keratinocyte, a cell death process that induces inflammation and scar formation by inhibiting the expression of TNF-α and β-catenin proteins. Thus, the electrospun PP scaffold with LPA can be potentially developed as a therapeutic avenue to target skin wound healing.
研究领域:
生物材料学
·
医学
·
组织工程学
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DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2016
·
IF 4.32
Yunzhu Qian,Hanbang Chen,Yang Xu,Jianxin Yang,Xuefeng Zhou,Feimin Zhang,Ning Gu
Constructing biomimetic structure and incorporating bioactive molecules is an effective strategy to achieve a more favorable cell response. To explore the effect of electrospinning (ES) nanofibrous architecture and collagen I (COL I)-incorporated modification on tuning osteoblast response, a resorbable membrane composed of poly(lactic-co-glycolic acid)/poly(caprolactone) (PLGA/PCL; 7:3 w/w) was developed via ES. COL I was blended into PLGA/PCL solution to prepare composite ES membrane. Notably, relatively better cell response was delivered by the bioactive ES-based membrane which was fabricated by modification of 3,4-dihydroxyphenylalanine and COL I. After investigation by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle measurement, and mechanical test, polyporous three-dimensional nanofibrous structure with low tensile force and the successful integration of COL I was obtained by the ES method. Compared with traditional PLGA/PCL membrane, the surface hydrophilicity of collagen-incorporated membranes was largely enhanced. The behavior of mouse preosteoblast MC3T3-E1 cell infiltration and proliferation on membranes was studied at 24 and 48 hours. The negative control was fabricated by solvent casting. Evaluation of cell adhesion and morphology demonstrated that all the ES membranes were more favorable for promoting the cell adhesion and spreading than the casting membrane. Cell Counting Kit-8 assays revealed that biomimetic architecture, surface topography, and bioactive properties of membranes were favorable for cell growth. Analysis of β1 integrin expression level by immunofluorescence indicated that such biomimetic architecture, especially COL I-grafted surface, plays a key role in cell adhesion and proliferation. The real-time polymerase chain reaction suggested that both surface topography and bioactive properties could facilitate the cell adhesion. The combined effect of biomimetic architecture with enhanced surface activity by 3,4-dihydroxyphenylalanine-assisted modification and COL I incorporation of PLGA/PCL electrospun membranes could successfully fill osteogenic defects and allow for better cell proliferation and differentiation.
研究领域:
生物材料
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组织工程
·
细胞生物学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Materials Today Bio
·
2024
·
IF 8.7
Xiang Han,Feiyang Wang,Yuzhuo Ma,Xuerong Lv,Kewei Zhang,Yue Wang,Ke Yan,Youmin Mei,Xiaoqian Wang
Traditional fibrous membranes employed in guided tissue regeneration (GTR) in the treatment of periodontitis have limitations of bioactive and immunomodulatory properties. We fabricated a novel nTPG/PLGA/PCL fibrous membrane by electrospinning which exhibit excellent hydrophilicity, mechanical properties and biocompatibility. In addition, we investigated its regulatory effect on polarization of macrophages and facilitating the regeneration of periodontal tissue both in vivo and in vitro. These findings showed the 0.5%TPG/PLGA/PCL may inhibit the polarization of RAW 264.7 into M1 phenotype by suppressing the PI3K/AKT and NF-κB signaling pathways. Furthermore, it directly up-regulated the expression of cementoblastic differentiation markers (CEMP-1 and CAP) in periodontal ligament stem cells (hPDLSCs), and indirectly up-regulated the expression of cementoblastic (CEMP-1 and CAP) and osteoblastic (ALP, RUNX2, COL-1, and OCN) differentiation markers by inhibiting the polarization of M1 macrophage. Upon implantation into a periodontal bone defect rats model, histological assessment revealed that the 0.5%TPG/PLGA/PCL membrane could regenerate oriented collagen fibers and structurally intact epithelium. Micro-CT (BV/TV) and the expression of immunohistochemical markers (OCN, RUNX-2, COL-1, and BMP-2) ultimately exhibited satisfactory regeneration of alveolar bone, periodontal ligament. Overall, 0.5%TPG/PLGA/PCL did not only directly promote osteogenic effects on hPDLSCs, but also indirectly facilitated cementoblastic and osteogenic differentiation through its immunomodulatory effects on macrophages. These findings provide a novel perspective for the development of materials for periodontal tissue regeneration.
研究领域:
牙科材料
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组织工程
·
免疫学
·
再生医学
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DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 30
·23181-23188
·
IF 3.84
Wenxiu Li,Hua Dong,Guannan Tang,Ting Ma,Xiaodong Cao
Janus and microcapsule particles are very attractive for drug delivery applications due to their capability of targeted and/or programmed drug release. In this paper, a facile yet robust strategy is reported for the first time to fabricate both Janus and microcapsule particles in a simple microfluidic device via the same protocol. According to the classic spreading and partial wetting theory, the key point to fabricate Janus and microcapsule particles in single emulsions is that the two immiscible components should undergo a precisely controlled phase separation, i.e., dewetting for Janus particles and wetting for microcapsule particles. Herein we show that this requirement can be satisfied simply by a subtle choice of organic solvents for the dispersed phase. As a model hybrid material, PLGA/PCL Janus and microcapsule particles are obtained via switching the organic solvent between dimethyl carbonate and dichloromethane. ATR-FTIR spectra and acetone treatment demonstrate that PLGA and PCL occupy their own hemisphere in the Janus particles. In contrast, the shell on the microcapsule particle surface is composed of only PLGA, and the core is composed of PCL in which tiny PLGA beads are embedded. The applications of PLGA/PCL Janus and microcapsule particles in drug delivery are characterized via an in vitro degradation test. The results prove that Janus and microcapsule particles exhibit distinct degradation behaviors, implying their capability of programmable drug delivery in different manners.
研究领域:
生物医学工程
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制药科学
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材料科学
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产品关联
DOI
高影响因子
RSC Advances
·
2013
·Vol. 4
·期 18
·9031-9038
·
IF 2.562
Delin Cheng,Xiaodong Cao,Huichang Gao,Xiaoling Ye,Wenxiu Li,Yingjun Wang
Composite polymer devices have gained considerable interest in biomedical applications. In this work, a composite poly(lactic-co-glycolic acid)/poly(ε-caprolactone) (PLGA/PCL) microsphere with a layered architecture and an island–sea topography was developed. PCL was found to occupy the shell layer of the microsphere, while PLGA constituted surface islands and the internal core. This subtle structure was derived from the different external and internal rates of phase separation between PCL and PLGA. The existence of the PLGA islands enhanced the hydrophilicity of the PCL shell. Mouse mesenchymal stem cells (mMSCs) were cultured and they grew well on the PLGA/PCL microspheres. The surface of the microsphere was microscopically composed of PCL sea and PLGA islands, and the mMSCs showed a trend to attach to the more hydrophilic PLGA islands and bridged between adjacent microspheres. This paper provides a new alternative to design functional microspheres for potential application in tissue repair.
研究领域:
生物医学工程
·
材料科学
·
细胞生物学
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产品关联
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2019
·
IF 4.959
Zetao Wang,Ruiming Liang,Xianfang Jiang,Jiali Xie,Peian Cai,Haimin Chen,Xintang Zhan,Danqing Lei,Jinmin Zhao,Li Zheng
Nanofibers as niche-biomimetic scaffolds hold promise in guided bone regeneration (GBR). Here we fabricated poly (lactic- co -glycolic acid) (PLGA)/poly(caprolactone) (PCL)-doped octacalcium phosphate (OCP) nanofiber membranes via electrospinning and investigated the osteogenic behavior of marrow mesenchymal stem cells (MSCs) on the membranes. By adjusting different ratio of OCP to PLGA/PCL, three hybrid stents including PLGA/PCL, PLGA/PCL/2 wt%OCP, PLGA/PCL/4wt%OCP were successfully prepared. The PLGA/PCL/OCP membranes showed a decrease in fiber diameter compared with PLGA/PCL, leading to enhanced mechanical strength. In-vitro studies showed that PLGA/PCL/OCP membranes better supported cell adhesion, spreading and proliferation than PLGA/PCL. The incorporation of OCP via electrospinning also endowed the membranes with osteoinductive capacity, as evidenced by activation of ALP activity, increased gene expression of bone specific markers (such as Runx2 , ALP , Col 1a1 , OPN , OCN , BMP2 ), and mineral nodules formation compared to PLGA/PCL. Comparatively, PLGA/PCL/4wt%OCP showed better mechanical and biological performance than PLGA/PCL/2 wt%OCP, demonstrating the role of OCP in nanofiber membranes. Thus, the electrospun PLGA/PCL/OCP nanofiber membranes can be potentially developed as a promising hybrid stent for GBR.
研究领域:
生物材料
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组织工程
·
干细胞生物学
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生物医学工程
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DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 97
·79969-79975
·
IF 3.84
Xiaodong Cao,Wenxiu Li,Ting Ma,Hua Dong
We report in this paper a one-step route for the preparation of core–shell, patchy, patchy Janus and Janus particles via a microfluidic-assisted phase separation process. As a proof of concept, PLGA/PCL hybrid particles are fabricated and the results show that the four types of particles can be harvested with high yield and narrow size distribution by precise control over the phase separation process, or namely, interfacial tensions and spreading coefficients between immiscible phases, after generation of the single emulsion in microchannels. This new strategy described herein is rather simple and versatile for producing a wide range of other particles with simultaneously controlled size, shape and anisotropy.
研究领域:
材料科学
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化学工程
·
生物医学工程
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产品关联
DOI
高影响因子
ACS Applied Bio Materials
·
2026
·
IF 5.6
Yonghao Xiao,Yuanguo Zhang,Yuehao Xing,Ming Gao,Yuting Ge,Hailei Li,Xin Wu,Lin Ye,Zeng-guo Feng
Bypass vascular grafting is an effective treatment for cardiovascular disease. Currently, small-diameter vascular grafts (SDVGs, inner diameter <6 mm) for peripheral revascularization, especially for lower limb revascularization, are in high demand. Previously, we prepared and investigated PLGA@PCL SDVGs with core/shell fibers. In this study, PLGA@PCL/elastin core/shell fibers (PCEs) SDVGs were further prepared by electrospinning, so as to enhance the vascular regeneration performance through the introduction of exogenous soluble elastin. The cross-linking effects of elastin with different cross-linking reagents on PCEs SDVGs were subsequently explored. Among them, PCEs SDVGs cross-linked with 1-ethyl-3-(3-(dimethylamino)propyl) carbodiimide (EDCI) and N-hydroxysuccinimide (NHS) retained the most soluble elastin content and achieved a compliance of 1.74 ± 0.62%/mmHg × 10–2, a bursting pressure of 1750 mmHg, and a tensile strength of 2.09 ± 0.51 MPa, which are mostly close to those of human saphenous vein. Subsequently, in vivo transplantation in the abdominal aorta showed that all implanted SDVGs cross-linked by EDC/NHS (PCEsEN) remained patent without aneurysm formation. Histological staining results further showed that the introduction of elastin not only promoted the degradation of SDVGs but also accelerated the formation of neointimal tissue and suppressed the calcification. Thus, PCEsEN shows promising potential to be translated into SDVGs in clinic.
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产品关联
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2020
·
IF 6.289
Xuefeng Zhou,Xi Cheng,Danlei Xing,Qi Ge,Yan Li,Xianghong Luan,Ning Gu,Yunzhu Qian
Bioactive synthetic scaffolds with 3D porous structure are the most attractive materials among various guided bone regeneration membranes. However, its osteogenic potential is still insufficient. This study was aimed to develop a Calcium surface-anchored Collagen I-PLGA/PCL scaffolds (PP/COL I-pDA-Ca) with enhanced osteogenicity. PP/COL I-pDA-Ca was electrospun from a collagen I-blended PLGA/PCL matrix and then modified by the chelation of Ca-ions via mussel-inspired polydopamine coating. PLGA/PCL, PLGA/PCL-polydopamine, PLGA/PCL-pDA chelated by Ca-ions were used as controls. Osteogenic effects of the scaffolds were examined using MC3T3-E1 cell culture. PP/COL I-pDA-Ca maintained 3D porous architecture with interconnected pores formed by randomly-oriented filamentous fibers and MC3T3-E1 cells cultured on it for 12 h or 24 h were more stretched and spread than those on the controls. PP/COL I-pDA-Ca significantly upregulated α10, α11 and β1 integrin expression after 48 h culture. ALP activity, OCN, OSX, BMP2 and RUNX2 expression of MC3T3-E1 cells cultured on PP/COL I-pDA-Ca scaffolds for 7 and 14 days were substantially enhanced when compared to controls ( p < 0.05). The pDA-based Ca chelation, COL I incorporation and 3D bionic structure promoted cell adhesion and osteogenic differentiation of MC3T3-E1 cells. This novel PP/COL I-pDA-Ca scaffolds is an attractive alternative for guided bone regeneration.
研究领域:
生物材料
·
组织工程
·
生物医学工程
·
骨科
✓ 已验证
DOI
高影响因子
BioNanoScience
·
2026
·Vol. 16
·期 5
·305
·
IF 4
Qian Yunzhu,Pan Xujie,Zhou Xuefeng,Ma Buyun,Liu Zhaolin,Cai Zhiyan
Bone regeneration in compromised environments, such as infected or inflammatory defects, remains a significant clinical challenge. This study addresses the limitations of curcumin (Cur), including poor solubility, low bioavailability, and limited antibacterial activity, by developing a series of curcumin-metal complexes through chelation reactions. These complexes were encapsulated into nanoliposomes and subsequently incorporated into poly(lactic-co-glycolic acid)/polycaprolactone (PLGA/PCL) electrospun scaffolds to construct multifunctional bone regeneration platforms. The fabricated scaffolds exhibited uniform nanofibrous morphology, enhanced mechanical properties, and improved surface hydrophilicity, which collectively promoted cell adhesion and proliferation. Among the complexes, Cur-Cu demonstrated the highest antioxidant activity and superior osteogenic performance, significantly enhancing alkaline phosphatase (ALP) activity and upregulating integrin β1/α1 gene expression in MC3T3-E1 cells. In contrast, Cur-Zn exhibited broad-spectrum antibacterial efficacy against both Escherichia coli and Staphylococcus aureus , while Cur-Ca showed limited functional benefits due to rapid ion release and potential cellular interference. These findings highlight the tailored functionality of Cur-metal complexes. Cur-Cu scaffolds are promising for repairing bone defects under oxidative stress, whereas Cur-Zn scaffolds are effective in preventing implant-associated infections. This work provides a rational strategy for designing microenvironment-responsive biomaterials with integrated antioxidant, osteogenic, and antibacterial properties for advanced bone tissue engineering.
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DOI
高影响因子
PLoS One
·
2026
·Vol. 21
·期 1
·e0337192
·
IF 2.6
Yonghao Xiao,Han Wang,Yuhao Jiao,Yuehao Xing,Lin Ye,Ai-ying Zhang,Xue Geng,Fanshan Qiu,Zengguo Feng,Hongbo Chen,Yongquan Gu
Cardiovascular disease has become the leading cause of death. It is the common goal for researchers worldwide to develop small-diameter vascular grafts (SDVGs) which could meet clinical needs. In this study, PLGA@PCL core-shell structural fibrous SDVGs was fabricated by coaxial electrospinning process, and then the surface heparinization of the vascular material was carried out after H2N-PEG-NH2 fixed on sodium hydroxide-treated electro-spun PCL tubes. Finally, the long-term patency and tissue regeneration of the grafts were evaluated in vivo through the rabbit carotid artery replacement model. The results indicate that the heparin-modified PLGA@PCL core-shell structural fibrous SDVGs achieved long-term patency and the arrangement of collagen and elastin in the neointima was similar to the native vessel in the rabbits after 9 months. After 3 months postoperatively, endothelialization was almost complete, and vascular calcification was also observed. It can be concluded that surface heparinization is a feasible modification method for in situ tissue-engineered vascular grafts, and controlling the occurrence of vascular calcification is another important issue to be solved in the development of SDVGs, and it is also the focus of our next research work.
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）