CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Yuhong Cui,Baoluo He,Shenghua Xue,Zhuo Chen,Shujuan Liu,Qian Ye,Feng Zhou,Weimin Liu
Improving the tribological performance of water via designing nanomaterials as lubricant additive has garnered mounting interest. In this work, the poly-(N-isopropylacrylamide) microgel is grafted onto Fe 3 O 4 nanoparticles surface (Fe 3 O 4 @PNA) via free radical polymerization. Then, the Fe 3 O 4 @PNA can self-anchored on the MXene nanosheets via hydrogen bonding interaction to obtain MXene@Fe 3 O 4 @PNA composite. The as-prepared MXene@Fe 3 O 4 @PNA can effectively reduce friction and wear when used as a water-based lubricant additive. Compared with pure water, the average coefficient of friction (COF) and wear volume decrease by 61% and 78.7%, respectively. The lubrication mechanism was analyzed by focused ion beam technique-TEM (FIB-TEM) and X-ray photoelectron spectroscopy (XPS). The outstanding tribological performance of MXene@Fe 3 O 4 @PNA is attributed to the synergistic lubrication effect of MXene and Fe 3 O 4 @PNA. Interlayer shear of MXene and spherical Fe 3 O 4 @PNA can make MXene@Fe 3 O 4 @PNA play a full role of sliding friction and rolling friction mechanism, which can induce tribo-chemical reactions to form a protective film on the contact area of the friction pair. In addition, the hydrophilic groups of the MXene@Fe 3 O 4 @PNA can adsorb a large number of water molecules and form a hydration layer, reducing the agglomeration of nanosheets and acting as a boundary lubrication film. Moreover, the as-prepared MXene@Fe 3 O 4 @PNA exhibit excellent photothermal conversion performance in both underwater environment and dry state. Based on this characteristic, infrared light can be collected efficiently and stored quickly. Then, convert and utilize it. Therefore, the multifunctional MXene@Fe 3 O 4 @PNA has a broad application prospect as a water-based intelligent lubrication additive.
研究领域:
材料科学
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纳米技术
·
摩擦学
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DOI
高影响因子
ACS Omega
·
2025
·
IF 4.3
Wenxi Zhu,Yuhan Luo,Haidong Li,Yufei Gong
In geothermal drilling, the significant rheological deterioration of biopolymer drilling fluids can lead to decreased cutting-carrying efficiency and fluctuations in annulus pressure, which can easily result in unpredictable downhole accidents. To address the challenges, this study innovatively introduces temperature-sensitive monomers (NIPAM) and nanosilica into molecular chains based on temperature response effects and adsorption stability to counteract the negative effects of temperature and salt ions, aiming to develop a rheological agent (PNA-SiO2) that is resistant to high temperature and salt. The molecular structure and thermal stability of PNA-SiO2 were characterized by FT-IR and TGA, and it was demonstrated to have the stability of the molecular main chain within 317 °C. According to rheological and filtration studies, PNA-SiO2 effectively addresses the issues of high-temperature viscosity attenuation and a sharp rise in the filtration volume of bentonite mud by aggressively adsorbing, creating a stable thixotropic network, and enhancing molecular repulsion. After aging at 60–180 °C, the drilling fluid with 2–4% PNA-SiO2 has excellent rheology (AV ≥ 57.5 mPa·s, PV ≥ 42 mPa·s, Gel10 min ≥ 1.533 Pa) and filtration (FLAPI ≤ 9.6 mL) properties that fully satisfy API requirements. The H–B model is the preferred model to accurately describe the rheology behavior of PNA-SiO2 drilling fluids. Moreover, under the comprehensive influence of Na+ on the interlayer spacing of bentonite and the thickness of the diffusion bilayer, PNA-SiO2 has applicability in high-temperature saturated saline water. After aging at 150 °C and 5–36% NaCl, the PNA-SiO2 drilling fluid exhibits stable viscosity (AV ≥ 55 mPa·s, PV ≥ 44 mPa·s) and low filtration volume (≤9.6 mL). In conclusion, PNA-SiO2 is used as a rheological and fluid loss agent, offering a cost-effective solution to the technical issues of severe leakage and inadequate cutting-carrying capacity encountered by geothermal drilling, which enables the safe and quick exploration of geothermal resources.
研究领域:
材料科学
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石油工程
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地质学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Kai Zhang,Meina Yang,Helin Sun,Xueyin Wang,Hao Li,Jinxiang Han,Zhongwen Zhang
Using Peptide nucleic acid (PNA), this work provides a unique approach for controlling CRISPR/Cas13a activity by RNA Inhibitory PNA (RIP). This method reduces the action of CRISPR/Cas13a by inhibiting the binding of crRNA to CRISPR/Cas13a, which was subsequently utilized to detect metalloproteinase-2 (MMP-2) activity. We carefully constructed a PNA sequence that includes a peptide sequence (Gly-Pro-Leu-Gly-Val-Arg-Gly) that is particularly cleaved by MMP-2 in the middle. When MMP-2 is not present in the system, the PNA/crRNA complex reduces crRNA binding to CRISPR/Cas13a, hence inhibiting CRISPR/Cas13a cleavage activity. When MMP-2 is present in the system, it cleaves the peptide sequence (Gly-Pro-Leu-Gly-Val-Arg-Gly) selectively and so prevents PNA from binding to crRNA, enabling crRNA to reach the "free" form. Then, in the presence of an RNA activator, crRNA may bind to CRISPR/Cas13a and stimulate its activity. CRISPR/Cas13a triggered in this manner may precisely cleave the DNA sequence containing modified -UU- bases on the electrode surface . Thus, the ferrocene (Fc) is changed away from the electrode surface at one end of the DNA , enabling for the recovery of the Electrochemiluminescence (ECL) signal from the two-dimensional material transformed on the electrode surface. As a result, MMP-2 activity was detected utilizing this technique of inhibiting crRNA activity with a detection limit of 15.56 fM. Our suggested method of inhibiting CRISPR/Cas13a activity via PNA has a broad variety of potential therapeutic applications in gene editing and the development of CRISPR/Cas13a-based biosensors.
研究领域:
分子生物学
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基因编辑
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生物传感技术
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DOI
高影响因子
BIOELECTROCHEMISTRY
·
2024
·
IF 4.8
Zhihao Xu,Zhixiang Lv,Huijiao Yang,Jiashuo Zhang,Zijie Sun,Dawei He,Ruijiang Liu
A label-free electrochemical DNA detection strategy based on self-assembled α-Fe 2 O 3 /Fe 3 O 4 nanosheets with PNA-DNA hybridization process was developed for ultrasensitive detection of APOE ε4 gene, one of the most robust genetic risks for Alzheimer’s Disease (AD). In this work, magnetic α-Fe 2 O 3 /Fe 3 O 4 heterogeneous nanosheets were prepared by hydrothermal-calcined reduction method and loaded with Au nanoparticles (AuNPs) on their surfaces. The magnetic α-Fe 2 O 3 /Fe 3 O 4 @Au nanocomposites significantly enhanced the electrochemical response as a signal amplification matrix and were able to bind to the magnetic glassy carbon electrode (MGCE) surface by magnetic self-assembly. Moreover, owing to the high specificity and stable binding capacity of PNA with respect to the target DNA, the biosensor not only enabled accurate (the limit of detection was estimated to be 0.147 pM) and rapid detection of the APOE ε4 gene, but also exhibited excellent specificity, stability and regeneration capability. Additional, the satisfactory recoveries were also obtained in real samples of human serum, ranging from 92.83 % to 106.22 % with relative standard deviation (RSD) between 0.25 % and 1.85 %. The results possessed important reference value for exploring the application of DNA biosensor technology in the diagnosis of APOE gene mutation.
研究领域:
生物传感器技术
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医学诊断
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分子生物学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
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2019
·Vol. 9
·期 66
·38298-38308
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IF 3.049
Xiaojun Xu,Shu Xing,Mengjia Xu,Pan Fu,Tingting Gao,Xiaokang Zhang,Yang Zhao,Chao Zhao
Screening epidermal growth factor receptor (EGFR) mutations, especially deletions, is essential for diagnosis of non-small cell lung cancer (NSCLC) and also critical to inform treatment decisions for NSCLC patients. Here, we demonstrated a facile peptide nucleic acid (PNA) microarray-based fluorometric method for sensitive and specific detection of EGFR mutation, using rolling circle amplification (RCA), graphene oxide (GO), and a fluorescently-labeled detection probe (F-DP). First, the EGFR gene sequence was efficiently captured by the label-free PNA probe which was attached on the surface of a 96-well plate. And then, the EGFR mutation sequence was specifically amplified by RCA using the circular DNA, which was formed by the ligation of the padlock probe when hybridizing with the EGFR mutation, as a template. The single-stranded RCA product (RCAP) was then sensitively detected with the F-DP and GO system. This method has a detection limit of 0.3 pM for EGFR mutation and a high discrimination capability to target EGFR mutation against EGFR wildtype. The use of a PNA microarray and a fluorescence quenching platform make this system quite suitable for high-throughput analysis of EGFR mutations in resource-limited settings without the need of costly and cumbersome equipment. Furthermore, this detection system provides a novel way for the diagnosis of other diseases that are caused by gene deletion mutations.
研究领域:
分子生物学
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癌症诊断
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ORGANIC ELECTRONICS
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2024
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IF 2.7
Cangyu Wang,Xiaoge Song,Sinyeong Jung,Wansi Li,Maggie Ng,Season Si Chen,Man-Chung Tang
Recent research on anthracene-based hydrocarbon (PNA) as the emissive layer host found that deuteration mediated the formation of these adduct species, presumably quenchers, and thus improved the device lifetime. Meanwhile, studies on deuterium in PNA host materials with stepwise deuteration are scarce. In this work, we report a systematic study of isotopic effects through independent deuteration of the phenyl or/and the dibenzofuran unit on a series of PNA materials polyaromatic dibenzofuran ( PAF , PAF- d 5 , PAF- d 7 and PAF- d 12 ). We fabricated fluorescent OLED based on this triplet-triplet up-conversion active host materials for conventional MR-TADF emitter. A short-lived excited delayed lifetime of less than 2 μs has been observed in their electroluminescence decay studies. In addition, LT 80 of up to 12.7 h has been observed in PAF- d 12 at the brightness of 1000 cd m −2 , corresponding to a 4-fold enhancement of OLED stability compared to the non-deuterated counterpart. We hope the current research can provide insights for further device improvement.
研究领域:
材料科学
·
有机电子学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2019
·
IF 3.973
Shu Xing,Xiaojun Xu,Pan Fu,Mengjia Xu,Tingting Gao,Xiaokang Zhang,Chao Zhao
Rapid and sensitive single nucleotide polymorphisms (SNPs) genotyping is of particular important for early diagnosis, prevention, and treatment of specific human diseases. A simple and low-cost SNP detection method would be valuable for routine analysis in resource-limited settings. Here, we demonstrated a novel and convenient gold nanoparticle (AuNPs) based colorimetric approach for efficient screening of SNPs at room temperature without instrumentation. SNP detection is performed in a single tube with one set of unmodified AuNPs, a label-free peptide nucleic acid (PNA) probe, a single exonuclease (S1 nuclease), and the target to be tested. S1 nuclease could digest DNAs in DNA/PNA duplexes involving a mismatch into small fragments, while DNAs in the fully-matched DNA/PNA duplexes can be effectively protected by PNA from enzymatic degradation. This difference could be easily discriminated by color changes associated with gold aggregation. PNA oligomers can induce immediate AuNP aggregation even in the presence of nucleoside monophosphates (dNMPs), the digestion products of DNA . Whereas PNA/DNA duplexes can effectively stabilize unmodified AuNPs, and the stabilization effect of PNA/DNA is better than single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Without the need of precise temperature control and extra salt addition, SNPs are detected with a detection limit of 2.3 nM in cell lysate. Moreover, this system can effectively discriminate a range of different mismatches even in spiked cell lysate, demonstrate the potential use of this biosensor for biological samples.
研究领域:
分子生物学
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纳米生物技术
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医学诊断
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Yirui Li,Zhipeng Wang,Xiaodong Sun,Qingqing Yang,Ghazala Ashraf,Bing Niu,Xueen Fang,Qin Chen
Nucleic acid amplification tests are widely used for molecular diagnostics. However, primer–dimer formation and nonspecific amplification may compromise analytical accuracy and increase the risk of false-positive results. Here, temperature-gated PNA@AuNRs (peptide nucleic acid–modified gold nanorods) were introduced to improve amplification specificity. A microwave-assisted modification method efficiently immobilized PNA onto AuNRs, enabling directional primer capture at low temperature and controlled release near polymerase-optimal conditions. PNA@AuNRs-LAMP achieved a limit of quantification (LOQ) of 102 copies/μL with strong linearity (R2 = 0.991), while PNA@AuNRs-qPCR reached 10 copies/μL with excellent linearity (R2 = 0.998). Mechanistic analysis demonstrated strong PNA–primer affinity and a distinct thermal-start effect at 60 °C, absent in traditional LAMP. In spiked equine serum and nasal samples, PNA@AuNRs effectively eliminated nonspecific amplification and improved detection accuracy. These results highlight PNA@AuNRs-gated amplification as a promising strategy for high-specificity molecular diagnostics.
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DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2012
·
IF 0.714
Xiaofang Deng,Liqiang Liu,Wenwei Ma,Chuanlai Xu,Libing Wang,Hua Kuang
In this study, two anti-peanut agglutinin (PNA) polyclonal antibodies were successfully prepared. Using mouse anti-PNA polyclonal antibody as the capture antibody and rabbit anti-PNA polyclonal antibody as the detection antibody, a novel sandwich enzyme-linked immunosorbent assay (ELISA) was developed to quantify PNA. The detection limit of the ELISA method was low (0.49 ng/mL), and the linear dynamic range was between 0.78 and 100 ng/mL. The recovery ranged from 93.86% to 139.3%, whereas the intra- and inter-assay coefficients of variation were less than 10.25% and 12.06%, respectively. Sample analysis verified this method as a reliable tool for the detection of PNA in processed foods.
研究领域:
免疫学
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食品科学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Shiwei Xu,Guofeng Wang,Yueyue Feng,Juanjuan Zheng,Liying Huang,Jiahao Liu,Yisha Jiang,Yajun Wang,Nannan Liu
MicroRNAs (miRNAs) are endogenous and noncoding single-stranded RNA molecules with a length of approximately 18–25 nucleotides, which play an undeniable role in early cancer screening. Therefore, it is very important to develop an ultrasensitive and highly specific method for detecting miRNAs. Here, we present a bottom-up assembly approach for modifying glass microtubes with silica nanowires (SiNWs) and develop a label-free sensing platform for miRNA-21 detection. The three-dimensional (3D) networks formed by SiNWs make them abundant and highly accessible sites for binding with peptide nucleic acid (PNA). As a receptor, PNA has no phosphate groups and exhibits an overall electrically neutral state, resulting in a relatively small repulsion between PNA and RNA, which can improve the hybridization efficiency. The SiNWs-filled glass microtube (SiNWs@GMT) sensor enables ultrasensitive, label-free detection of miRNA-21 with a detection limit as low as 1 aM at a detection range of 1 aM–100 nM. Noteworthy, the sensor can still detect miRNA-21 in the range of 102–108 fM in complex solutions containing 1000-fold homologous interference of miRNAs. The high anti-interference performance of the sensor enables it to specifically recognize target miRNA-21 in the presence of other miRNAs and distinguish 1-, 3-mismatch nucleotide sequences. Significantly, the sensor platform is able to detect miRNA-21 in the lysate of breast cancer cell lines (e.g., MCF-7 cells and MDA-MB-231 cells), indicating that it has good potential in the screening of early breast cancers.
研究领域:
生物医学工程
·
分子生物学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
Sustainable Energy & Fuels
·
2023
·Vol. 7
·期 15
·3716-3726
·
IF 5.6
Wansi Lin,Yue Wang,Junhua Zhang,Huai Liu,Lincai Peng
Lignin is a highly abundant biopolymer next to only cellulose, and its transformation into high value-added products is always of interest. It is well known to contain bounteous hydroxyl groups, which can act as natural organic ligands to assemble metal catalysts. Herein, lignin was selected to assemble well-dispersed Zr nanoparticles in polyacrylonitrile nanofiber by electrospinning technology (ZrNPs-lignin/PAN). Systematic characterizations revealed that lignin promoted the coordination between zirconium ions and PAN nanofibers to generate Lewis acid and Lewis base sites. The abundant Lewis acid–base sites confer the composite with remarkable catalytic activity in the transfer hydrogenation (TH) of furfural (FAL) into furfuryl alcohol (FOL), and a quantitative yield of 95.9% with a high TOF of 15.1 h−1 was obtained under mild conditions. ZrNPs-lignin/PAN exhibited good recycling stability and excellent universality in the CTH of a diverse array of carbonyl chemicals into their corresponding products.
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产品关联
DOI
高影响因子
ACS Omega
·
2023
·
IF 4.1
Shasha Huang,Yulong Shan,Xiaoyan Shi,Zhongqi Liu,Qiang Wang,Hong He
In the present work, we report on two passive NOx adsorber (PNA) material candidates: the novel support CeSnOx with and without Pd loading. The NOx adsorption and storage capacities of fresh and hydrothermally aged CeSnOx and Pd/CeSnOx were investigated. The results show that CeSnOx exhibits a rather large NOx uptake and storage capacity (28.9 μmol/g), while the loading of Pd on CeSnOx can further increase the storage capacity to 37.6 μmol/g and affect the desorption temperature of NOx. It was found that the NOx desorption temperature of Pd/CeSnOx was compatible with the efficient operating window of selective catalytic reduction (SCR) catalysts. After a hydrothermal aging treatment at 800 °C for 12 h, the NOx adsorption and storage capacities of CeSnOx and Pd/CeSnOx increased, indicating excellent hydrothermal stability. The interaction of Pd with CeSnOx, the state of Pd species, and the structure of CeSnOx and Pd/CeSnOx are studied by combination of the characterization results.
研究领域:
化学
·
材料科学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）