ANALYST
·
2025
·
IF 3.3
Lingling Li,Doudou Lei,Pengyue Song,Qingbin Xu,Lihua Huang,Xiao Ma,Lidong Zhou,Ping Zhang,Weijun Kong
Sensitive detection of carbendazim (CBZ), a widely monitored bactericidal pesticide globally, is critical to reduce risks to human health. In this study, Eu ions were embedded into a rigid metal–organic framework (MOF), to prepare Eu-MOF as a fluorescence signal source, which was subsequently post-modified with polyethyleneimine (PEI) for efficient coupling with anti-CBZ monoclonal antibodies (mAbs). On this basis, a PEI@Eu-MOF-mAbs fluorescent probe-based lateral flow immunosensor (LFIS) was developed for CBZ analysis, enabling dual-mode detection: rapid visual qualitative observation by the naked eye and intelligent fluorescence quantitative analysis by using a smartphone. PEI modification not only effectively improved the zeta potential, fluorescence intensity, quantum yield, and biological coupling ability of the Eu-MOF to mAbs, but also significantly enhanced the stability and anti-interference performance of PEI@Eu-MOF. The introduction of PEI@Eu-MOF-mAbs probes characterized by strong fluorescence emission, long fluorescence lifetime, and low background interference, markedly improved the detection sensitivity of the LFIS platform for trace CBZ. Under optimal conditions, the detection limit for smartphone-assisted fluorescence analysis was as low as 1.3 pg mL−1 with a wide linear detection range of 0.25–125 ng mL−1 for CBZ. The recoveries in spiked Panax notoginseng samples were 93.40–106.0% with RSD <1.69%, indicating outstanding reliability of the newly developed LFIS platform for accurate detection of CBZ. In comparison with seven other common pesticides, CBZ produced significantly weakened and stable fluorescence, indicating good specificity and stability of the developed platform over 4 weeks. Compared with current analytical methods, the smartphone-assisted PEI@Eu-MOF-mAbs dual-modal LFIS platform exhibited advantages of simple construction, easy operation, rapid response, obvious visualization to the naked eye, and intelligent quantitative analysis, highlighting its broad application potential for rapid screening and accurate point-of-care detection of CBZ in a large number of foods and agricultural products.
研究领域:
分析化学
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食品安全
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生物传感
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免疫学
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环境监测
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药物残留检测
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纳米材料应用
·
即时检测技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHYSICAL ORGANIC CHEMISTRY
·
2016
·Vol. 30
·期 1
·e3584
·
IF 1.515
Chuanming Yu,Bingcheng Hu,Cheng Liu,Jiting Li
A kind of basal diarylethene through an easy synthetic procedure was synthesized and characterized, and a Suzuki coupling reaction was frequently operated to obtain the diarylethenes derivatives with some vivid functional groups like bromo, amido and pyridine. Two methods for derivatives of these switches contain porphyrins were described. All of their spectroscopic and photochromic properties were studied and intercompared. It is indicated that the difference in electron densities of the π-conjugated system of the closed forms has effects upon the absorption maxima in visible region of UV–Vis spectra. Dithienylcyclopentenes linked to porphyrins could emit luminescence substantially that display potentials to be used in the applications of nondestructive readout upon binary data storage and smart materials.
研究领域:
有机化学
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材料科学
·
光化学
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DOI
高影响因子
JOURNAL OF PHYSICAL ORGANIC CHEMISTRY
·
2015
·Vol. 28
·期 6
·428-430
·
IF 1.38
Qiuxia Wu,Xuming Zheng,Kemei Pei
Phototransformation mechanism of monuron induced by the triplet benzophenone in aqueous solution was investigated by laser flash photolysis technique, while quenching of the triplet benzophenone by monuron was studied by nanosecond transient absorption spectroscopy. The transient species in the transient absorption spectra have been identified by quantum chemistry calculations. The results show that under 355-nm irradiation proton transfer between the triplet benzophenone and monuron happens, and monuron radical is formed and followed by dechlorination degradation reactions. This work indicated that in aqueous solution some dissolved natural organic matters are helpful to degradation of the organic pollutants under the sunlight. Copyright © 2015 John Wiley & Sons, Ltd.
研究领域:
环境科学
·
光化学
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产品关联
DOI
高影响因子
JOURNAL OF PHYSICAL ORGANIC CHEMISTRY
·
2012
·Vol. 26
·期 1
·23-29
·
IF 1.963
Chang-Di Chen,Wen-Bing Sheng,Guo-Jun Shi,Can-Cheng Guo
The regioselective hydroxylation of the aromatic C–H bond on a series of naphthalenes with different β-substituent R (R = H, Me, Et, i -Pr, OMe, COOH, Br, etc.) was studied, and the substituent effect on the regioselectivity was investigated. The electron-donating substituent afforded the aromatic C–H bond hydroxylation at the 1α position with more than 80% selectivity, while the electron-withdrawing substituent afforded the aromatic C–H bond hydroxylation at the 4α position with more than 60% selectivity of β-substituted naphthalene hydroxylated with metachloroperbenzoic acid catalyzed by tetrakis(pentafluorophenyl)porphyrin manganese(III) chloride. The research showed that the steric and electronic effects of the substituent appeared to play a significant role in determining the regioselectivity, and the electronic effect was of more importance than the steric effect of the substituent in the current situation. The studies may provide additional proofs for the stepwise mechanism of the aromatic C–H bond hydroxylation through a cationic intermediate. Copyright © 2012 John Wiley & Sons, Ltd.
研究领域:
有机化学
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催化化学
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DOI
高影响因子
JOURNAL OF PHYSICAL ORGANIC CHEMISTRY
·
2013
·Vol. 26
·期 6
·460-466
·
IF 1.578
Hang Chen,Lu Jia,Xuan Xu,Jianyong Mao,Yong Wang,Congmin Wang,Haoran Li
For conventional organic substituents, an almost linear relationship can be established between the para Hammett constants in aqueous solutions and chemical shifts or natural population analysis charges (NPA charges). Based on these correlations, the Hammett constants of six synthesized ionic-pair substituents were estimated via the chemical shifts, which were well in agreement with the results calculated by NPA charges. The para Hammett constants of 89 different ionic-pair substituents (77 anion-cationic and 12 cation-anionic) were therefore calculated in the gas phase based on the density functional theory method. The results show that both the anion-cationic and cation-anionic substituents are electron-withdrawing groups and the different cation–anion combinations could tune the Hammett constants of the ionic-pair substituents in a range from 0.03 to 0.77. Copyright © 2013 John Wiley & Sons, Ltd.
研究领域:
有机化学
·
理论化学
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产品关联
DOI
高影响因子
JOURNAL OF PHYSICAL ORGANIC CHEMISTRY
·
2014
·Vol. 27
·期 6
·498-503
·
IF 1.245
Yimei Tang,Xiaoling Hu,Ping Guan,Xiangping Lin,Xiaoqian Li
Using microwave-assisted synthesis method, a series of paramagnetic ionic liquids comprising 1-vinyl-3-alkylimidazolium VRIM + cation and tetrahalogenidoferrate (III) FeCl m Br 4 − m − anion were designed and synthesized. The structure was analyzed using 1 H NMR and Raman spectroscopy. Ultraviolet–visible absorption spectra, thermal stability, magnetic susceptibility, viscosity, ionic conductivity, and solubility were characterized. Results show that elongation of the alkyl chain leads to replacement of bromides with a small amount of chlorides in the anion, shifting of UV maximum absorption peaks to shorter wavelengths, reduction of ionic conductivity, and solubility in polar solvents, as well as increase in fluidity, magnetic susceptibility, and solubility in nonpolar solvents. Copyright © 2014 John Wiley & Sons, Ltd.
研究领域:
化学
·
材料科学
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产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Pengyue Song,Doudou Lei,Lingling Li,Nan Long,Qingbin Xu,Ying Li,Lidong Zhou,Ruile Pan,Weijun Kong
Regarding the high incidence of residue and serious toxicity of acetochlor (ATC) pesticide, it is urgent to explore simple and low-cost methods for their sensitive detection in food. In this study, with zeolite-like imidazole frameworks (ZIFs) as the carriers for embedding glutathione-modified luminescent gold nanoclusters (GSH-AuNCs) exhibiting aggregation-induced emission (AIE) effect, a GSH-AuNCs@ZIF-8 probe driven strip-based lateral flow immunosensor (LFIS) was developed for visual qualitative and fluorescent quantitative dual-modal detection of ATC. The encapsulation of GSH-AuNCs into ZIF-8 with restriction effect not only significantly improved the stability and anti-interference ability of AuNCs, but also remarkably enhanced the fluorescence efficiency for signal amplification due to the enhanced AIE effect of GSH-AuNCs, endowing the LFIS platform with unique performance. Under optimal conditions, the LFIS platform could realize the point-of-care visualization and quantitation of ATC within 20 min with a detection limit of 8.3 pg/mL and a broad linear range of 0.01–10.0 ng/mL. High recoveries of 96.44%–106.8 % in the spiked ginger samples confirmed the outstanding accuracy and dependability of the new platform for ATC analysis of real samples. Compared with other immunoassays, the AuNCs@ZIF-8-based dual-modal LFIS exhibited the advantages of simple preparation, simple operation, short analytical time, and low cost, which could be expanded to monitor more pesticides and more trace contaminants in diverse food and agricultural products, and medicinal material matrices.
研究领域:
食品安全
·
分析化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2021
·Vol. 34
·期 2
·294-301
·
IF 3.223
Chang Wang,Jingyuan Yu,Lijuan Qi,Jiaxue Yu,Meiting Yang,Yan Du
We realized the user-friendly and field-based point-of-care detection on a self-made portable device with the aid of personal glucose meter (PGM) for single nucleotide polymorphisms (SNPs) of melanoma and colon cancer marker BRAF V600E with good sensitivity and selectivity by integrating the loop-mediated isothermal amplification (LAMP) reaction and the strand exchange signal transduction. The device is equipped with a rotating magnetic field as well as a heating element, under which the pre-added magnetic nanochains (MNCs) help to homogenize the reaction mixture and overcome the low diffusion efficacy of the reactant and the required temperature for different reactions can be met.
研究领域:
生物技术
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医疗器械
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癌症研究
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纳米技术
·
分子诊断
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产品关联
DOI
高影响因子
ACS Applied Bio Materials
·
2020
·
IF 0
Zhenguo Yu,Zhentan Lu,Jiangxi Huang,Jiaqi Zhang,Yu Huang,Wenwen Wang,Yuanli Chen,Ke Liu,Dong Wang
Bacterial contamination in water is a serious health risk to human beings, so it is very important to realize the point-of-care (POC) bacterial detection in water. However, the traditional bacterial detection methods are time-consuming, professional- and equipment-dependent, and do not meet the needs of POC detection. There is a pressing need to develop a platform for POC bacterial detection to defeat the increasing risk of bacterial infections. Herein, a surface functional nanofiber membrane (NFM) is prepared by layer-by-layer (LBL) self-assembly as a platform for POC detection of bacterial concentration; it is naked-eye visualization and ultrasensitive. The platform shows obvious bacterial responsiveness, which allows naked-eye visualization of bacterial concentration (102–106 CFU/mL) within 30 min and can quantitatively detect the bacterial concentration (101–106 CFU/mL) by fluorescence within 5 min. The platform not only exhibits high efficiency but also has a low threshold for bacterial concentration detection. Furthermore, the platform shows good consistency with traditional methods in the detection of bacteria in practical water samples, and has the potential for use in detecting bacterial concentrations in water supplies to protect human beings from health hazards. This work also provides useful reference for research on bacterial detection, taking advantage of the surface characteristics of bacteria and the high sensitivity of NFM.
研究领域:
材料科学
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生物医学工程
·
环境科学
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产品关联
DOI
PubMed
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Jingcheng Zheng,Jie Chen,Ruifen Sun,Weiwei Zhang,Yun Huang,Jiwei Shao,Yuwu Chi
Fluorescent immunochromatographic test (FICT) strips have received extensive attention due to their virtue of simple operation, low cost, and a point-of-care (POC) assay. In recent years, people are constantly seeking new labels to improve the sensitivity of immunosensors. Perovskite nanocrystals (PNCs) possess strong fluorescence and a narrow emission peak, making them an excellent choice as a new fluorescent signal label. Herein, we have developed a simple, fast, and environmentally friendly method to synthesize CsPbBr 3 /CsPb 2 Br 5 @SiO 2 with the good water stability and high biocompatibility. These CsPbBr 3 /CsPb 2 Br 5 @SiO 2 have been applied to construct FICT strips. To achieve the quantitative determination of human chorionic gonadotropin (HCG), a lab-made FICT strips detection system was established. The FICT strips had a good linear relationship to HCG in the range of 5–500 ng/mL, and the detection limit was 2.09 ng/mL. In addition, the recovery results of HCG detection in human urine samples also proved the feasibility of this FICT strips. CsPbBr 3 /CsPb 2 Br 5 @SiO 2 can also be modified with different biomarkers for functionalization, which exhibits a promising application in the development of sensing platform of FICT strips.
研究领域:
分析化学
·
材料科学
·
生物传感
·
纳米技术
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产品关联
DOI
高影响因子
TALANTA
·
2021
·
IF 6.057
Qichao Ye,Chen Men,Lili Tian,Yuxin Liu,Lei Zhan,Yuan Fang Li,Cheng Zhi Huang,Shu Jun Zhen
Conventional methods for the detection of the sulfur mustard poisoning metabolic marker, thiodiglycol (TDG), require expensive instruments and reagents as well as professional operators. To address these problems, a novel test strip based on a molecularly imprinted sensitive membrane (MIM) was developed in this work for point-of-care (POC) detection of TDG. The TDG test strip was prepared conveniently by coating molecular imprinted polymers (MIPs) on a nitrocellulose membrane. When the sample contained TDG, the MIPs could specifically bind with TDG. A great number of AuNPs (AuNPs) could then be adsorbed on the test strip via the formation of an Au–S bond between TDG and AuNPs, giving the test strip the obvious red color of AuNPs. In the absence of TDG, the test strip exhibited much lighter color because it could not adsorb AuNPs. By monitoring the color change of the test strip, TDG could be detected from 1.0 ng/mL to 100.0 μg/mL with a detection limit of 0.23 ng/mL (3σ) under the optimal conditions (the molar ratio of TDG to MAA was 1:2; the eluent was chloroform; the elution time was 50 min; the reaction time between MIPs and TDG was 15 min; the adsorption time of AuNPs was 40 min; the temperature of the reaction system was 35 °C). This method has excellent selectivity and has been used to detect TDG in urine, showing great potential for POC detection of TDG in clinical samples.
研究领域:
分析化学
·
生物传感器
·
即时检测
·
毒理学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Yaqin Zhang,Jianai Chen,Fange Kong,Chunxia Wang,Hangyu Guo,Yingchun Li,Jiahui Lu,Jicheng Zhang,Jiasi Wang,Yulin Zhou
Viral infections, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are some of the most dangerous threats to humans. SARS-CoV-2 has caused a global pandemic, highlighting the unprecedented demand for rapid and portable diagnostic methods. To meet these requirements, we designed a label-free colorimetric platform that combines the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated proteins (Cas) 12a system for naked-eye detection (named LFP). This method utilizes reverse transcription loop-mediated isothermal amplification (RT-LAMP) and the trans-cleavage activity of the CRISPR/Cas12a system to increase the sensitivity and specificity of the reaction. This platform can detect as few as 4 copies/μL of RNA and produces no false positive results when tested against the influenza virus. To better meet the requirements of point-of-care (POC) detection, we developed a portable device that can be applied in resource-poor and densely populated regions. The LFP assay holds great potential for application in resource-limited settings, and the label-free gold nanoparticle (AuNPs) probe can reduce costs, making it suitable for large-scale screening. We expect that the LFP assay will be promising for the POC screening of COVID-19.
研究领域:
分子生物学
·
生物技术
·
病毒学
·
纳米技术
·
诊断方法
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）