Sensing and Bio-Sensing Research
·
2024
·
IF 5.4
Lei Wang,Nana Jin,Meixuan Li,Jianhan Lin
Point-of-care testing (POCT) of pathogenic bacteria at low concentrations is vital to early warning of bacterial contaminations. A disposable centrifuge tube was reconstructed in this study for sensitive Salmonella detection in large-volume samples, where the entire bacterial detection progress from separation to detection, was performed within the tube. The bottom of this centrifuge tube was assembled with a flexible stirrer containing a pair of circular magnets, which was rapidly rotated using a DC motor to produce the vortex for simultaneous mixing and capture of target bacteria. Besides, immune manganese dioxide nanoflowers were synthesized and used to label target bacteria, followed by mimicking catalyze colorless TMB substrate into blue TMBox product. The product image was captured and analyzed by a smartphone App to quantitatively determine the target bacteria. This POCT centrifuge tube effectively achieved a separation efficiency of approximately 80 % for target bacteria from a 10 mL sample, enabling the detection of target bacteria within the range of 1.3 × 10 1 to 1.3 × 10 4 CFU/mL in 1 h, with a low detection limit of 13 CFU/mL. More importantly, this reconstructed centrifuge tube demonstrated its potential as a laboratory consumable for bacterial detection in routine screening owing to the features of low cost, easy operation and high integration.
研究领域:
微生物学
·
生物技术
·
即时检测
·
分析化学
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产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Xiying Chen,Haijuan Qin,Xiao Zhou,Weiran Li,Jingjing Zhang,Shuo Wang,Yaqing Liu
Azodicarbonamide (ADA) in flour products is easily converted to semicarbazide which greatly threatens human health. Herein, a long-lasting chemiluminescence (CL)-based biosensor was developed for quantitative point-of-care testing (POCT) of ADA. The threonine (Thr)-functionalized Cu-hemin MOFs (Cu-hemin@Thr) could induce persistent CL of luminol with excellent stability. The CL intensity was related to the competition reaction among ADA and a composite of glutathione-silver ions (GSH-Ag + ). In the presence of ADA, GSH is oxidized to glutathione disulfide (GSSG), which breaks the coordination between Ag + and GSH. The CL of the sensing system is then decreased which is expected to be used for ADA detection. By combining a homemade portable device as a detector and a smartphone as an analyzer, quantitative POCT of ADA was successfully achieved. The limit of detection was 0.562 μM (0.065 ppm), which is much lower than the maximum permissible concentration of ADA (45 ppm) in flour extract. The developed strategy demonstrated quantitative POCT capabilities along with advantages of low cost, excellent selectivity, and repeatability, presenting great potential application in food safety and environment monitoring.
研究领域:
分析化学
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食品安全
·
环境监测
·
生物技术
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2021
·Vol. 146
·期 15
·4775-4780
·
IF 4.616
Yu Ning,Fan Lu,Yucheng Liu,Sisi Yang,Fubing Wang,Xinghu Ji,Zhike He
Cholesterol is an essential compound for human health, and a high or low concentration of cholesterol is closely related to various diseases. Thus, developing a simple method for POCT of cholesterol has great significance in clinical diagnosis. In this work, alginate (Alg) hydrogels with glow-type chemiluminescence (CL) were prepared and applied for rapid and quantitative cholesterol detection via a smartphone. The glow-type CL hydrogels (HRP/COD/luminol/Alg hydrogels) contained luminol as a chemiluminescent reagent, horseradish peroxidase (HRP) and cholesterol oxidase (COD) for enzymatic cascade reactions. The HRP/COD/luminol/Alg hydrogels exhibited outstanding stability, which effectively avoided the enzyme inactivation during long-term storage. Furthermore, the HRP/COD/luminol/Alg hydrogels exhibited longer and more stable glow-type CL. With the help of COD catalytic specificity for cholesterol and bi-enzymatic cascade reactions, the glow-type CL hydrogels realized the specific and sensitive detection of cholesterol. The smartphone was used as a detector instead of a special large instrument for responding to the glow-type CL emission, and a LOD of 7.2 μM was obtained. Therefore, the proposed sensor expands the application of the glow-type CL in POCT and provides an alternative way for cholesterol detection in clinical diagnosis.
研究领域:
临床诊断
·
生物传感
·
医学检测
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DOI
高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 2
·94
·
IF 5.3
Cai Minghui,Zhang Wenwen,Yao Haixu,Su Huiyu,Cao Yang,Li Jiaxin,Min Xinyu,Cui Feiyun,Zhu Hui
Thrombotic diseases remain a major global health concern with high morbidity and mortality. Four biomarkers (TAT, t-PAIC, TM, PIC) enable real-time monitoring of coagulation, addressing limitations of traditional diagnostics. Herein, we developed a multiplex electrochemical immunosensor for the simultaneous detection of these biomarkers. The sensing platform is constructed on a glassy carbon electrode modified with gold nanoparticles and laser-induced graphene (AuNPs-LIG), which enhances conductivity and signal output. A “One-for-Four” strategy was employed using four distinct redox probes (AQ, OAP, MB, PPD), each conjugated to a specific antibody via amidation and anchored to functionalized carbon nanotubes (Au@CNTs), forming a multichannel signal amplification system with high sensitivity. The immunosensor exhibited excellent linearity (10–100 ng/mL, R 2 > 0.98) and low detection limits: 3.99 ng/mL (TAT), 5.25 ng/mL (t-PAIC), 2.51 ng/mL (TM), and 4.20 ng/mL (PIC). It also demonstrated high selectivity, reproducibility and long-term stability. Recovery in human plasma ranged from 84.1% to 112.21%, indicating strong applicability to complex clinical samples. This work presents a robust, portable POCT platform with great potential for early diagnosis and dynamic monitoring of thrombotic diseases. Graphical
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Xiying Chen,Xiaomin Wang,Yuan Fang,Liule Zhang,Minyang Zhao,Yaqing Liu
Bacterial infections seriously threaten human health and also bring huge financial burden. It is critical to construct multifunctional platforms for effectively inactivating bacteria right after point-of-care testing (POCT). Chemiluminescence (CL) bioassays are considered as powerful candidates for POCT as they are free from using an excitation light source, while the flash-type emission limits their further application. Herein, a CL system with long, persistent, and intensive intensity was constructed based on the peroxidase-like property of 4-mercaptophenylboronic acid (MPBA)-functionalized CuSe nanoprobes (CuSeNPs@MPBA), which improved the detection accuracy and sensitivity. By further integrating a smartphone as an analyzer, quantitative POCT of bacteria was realized with high sensitivity. The limit of detection was as low as 1.25 and 1.01 cfu mL–1 for Staphylococcus aureus and Escherichia coli detection, respectively. Specifically, bacteria can be eliminated with high efficiency due to excellent photothermal property of CuSeNPs@MPBA. The developed multifunctional platform also has advantages of simple operation with low cost, suggesting its high potential for use in food safety, environment monitoring, and clinical applications.
研究领域:
医学诊断
·
生物技术
·
公共健康
✓ 已验证
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Yang Liu,Yan Huang
MicroRNAs (miRs) are believed to be associated with cancer and serve as a marker of its presence. Detecting miR in body fluid with a high level of sensitivity is a significant factor for disease screening. A point-of-care technology (POCT) based electrochemiluminescence (ECL) biosensor was created with a higher level of sensitivity for the recognition of miR-126. Glutathione-stabilized gold nanoclusters (GSH-Au NCs) were employed as ECL reagents in this study. Potassium persulfate (K 2 S 2 O 8 ) was chosen as the co-reaction reagent. Consequently, we chose the catalytic hairpin self-assembly strategy to attain accurate detection of miR-126. The prepared sensor is able to detect miR-126 linearly from 10 aM to 0.1 nM, and the detection limit is as low as 3.17 aM.
研究领域:
医学诊断
·
生物传感技术
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产品关联
DOI
高影响因子
VACUUM
·
2024
·
IF 3.8
Xun Zhou,Xin Yi,Zhixian Liang,Jie Zhang,Guixiang Li,Yuanliang Tang,Kun Zhou,Shaohui Zhang
In this work, the photothermal effect of aminomodified MWCNTs/TiO 2 /SiO 2 /PDMS nanocomposites was utilized as a photothermal substrate to replace the metal heating block of traditional qPCR combining of the advantages of high photothermal effect of composite materials and low sample volume of static microfluidic PCR. Meanwhile, Multiple PCR rapid thermal cycles under 808 nm laser irradiation was accomplished through a non-contact energy conversion method and the use of chamber-immobilized PCR chips as specific reaction vessels. Notably, the chip chamber architecture requires merely about 8 μL of reagents compared to traditional PCR tubes. While reducing the amount of PCR reagents used, the higher specific surface area results in a larger contact surface (SSA) between the heat source and the solution. Thereafter, the CCD image sensor is then used as a fluorescence detector to monitor the amplification of the target DNA during the PCR reaction, thus enabling simultaneous fluorescence detection. In addition, the threshold cycle (Ct) value of qPCR and standard PCR curves for SARS-CoV-2 virus have been analysed in depth. Importantly, this study has important implications for the development of low-cost materials, miniaturized and portable Point-of-Care Testing (POCT) chips/systems.
研究领域:
生物技术
·
医学检测
·
材料科学
✓ 已验证
DOI
高影响因子
Journal of Analysis and Testing
·
2021
·Vol. 5
·期 1
·19-29
·
IF 0
Liu Zhong-gang,Xiao Meng,Yang Rong-zhe,Zhou Qiao-qiao,Ye Hui-fen,Yi Chang-qing
This work reports on a smartphone-based quantitative POCT system which consists of a paper-based microarray chip, a homemade LEGO dark-box, and a smartphone installed with custom-designed FChip-APP, for on-site quantitation of Fe 3+ , Vitamin 12 (VB 12 ), and folic acid (FA). Collection and automatic analysis of fluorescence turn-off response of three kinds of fluorescent nanoprobes which are coated onto filter papers by a smartphone-based device is the principle behind this study. The POCT system exhibits good analytical performance for multiplexed analysis of Fe 3+ , VB 12 and FA with a wide linear range (Fe 3+ , 1.0–14.0 μg mL −1 ( R 2 = 0.983); VB 12 , 1.35–40.0 μg mL −1 ( R 2 = 0.991); FA, 2.0–26.0 μg mL −1 ( R 2 = 0.994)) and low detection limit (Fe 3+ , 0.35 μg mL −1 ; VB 12 , 0.47 μg mL −1 ; FA, 0.56 μg mL −1 ). The cost and weight of the whole system are ~ $220 and ~ 600 g per set, highlighting its cost-effectiveness and portability. The simple operation procedures and user-friendly interface of the FChip-APP validate the ease-of-use feature of the reported system. This versatile smartphone-based quantitative POCT system provides a cost-effective and easy-of-use method for screening the context-specific causes of anemia and thus its effective interventions in the future, especially in resource-limited areas.
研究领域:
医学检测
·
生物传感
·
移动健康
✓ 已验证
产品关联
DOI
ANALYST
·
2021
·Vol. 146
·期 16
·5074-5080
·
IF 4.616
Fan Lu,Sisi Yang,Yu Ning,Fubing Wang,Xinghu Ji,Zhike He
Diabetes mellitus has received much attention because its complications include liver, kidney, eye, heart and cerebrovascular diseases. Thus, it would be highly significant to develop a rapid and efficient method for glucose detection in biological samples. In this work, a point-of-care testing (POCT) method of glucose detection was proposed using a standard colorimetric card for semi-quantitative determination patterns. In the prepared fluorescence color card for glucose, a good linear relationship was acquired by plotting the ratio of the grayscale value (I/I0) versus the logarithm of glucose concentration within 100.0 to 1000.0 μmol L−1, and the LOD of glucose detection was 1.1 μmol L−1. A large number of actual samples (30 serum and 7 urine) were analyzed and the results demonstrated that this method had good potential to be applied in the primary screening of diabetic patients. In addition, this method is universal and can be applied in the simultaneous detection of multiple small molecules. It provides a new strategy for the primary screening of multiple diseases simultaneously, which presents excellent application potential.
研究领域:
分析化学
·
医学诊断
·
即时检测
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Weiqi Xie,Jinbo Wu,Xiaoyi Xie,Mengli Wu,Menghan Zhang,Mengyao Zhang,Xiaoquan Li,Li Wang,Lanju Wang,Zhigang Liu,Zhizeng Wang,Chunxia Chen
The early diagnosis of ovarian cancer remains dissolved due to the lack of detection methods that are sensitive, rapid and accessible. To address this clinical challenge, this study developed an innovative diagnostic platform integrating a novel selenium nanoparticles (SeNPs) probe, dual-biomarker (CA125 and HE4) detection and intelligent analysis system. The detection limits for CA125 and HE4 were determined to be 35 U/mL and 100 pmol/L respectively, and no cross-reactivity was observed. Capitalizing on computer vision and AI prompt engineering technology, this study implemented smartphone-based image acquisition combined with AI-assisted analysis. The results of 360 clinical samples testing indicated that the coincidence rate of this kit with chemiluminescence method was over 95%, and the AUC of the combined detection of dual markers reached 0.914. By integrating the ROMA algorithm, the risk probability of ovarian cancer can be calculated in real time based on the test results and the patient's menopausal status, enabling quantitative risk assessment. This study provides a stable, highly specific, cost-effective and clinically decision supported point-of-care diagnostic solution for the early screening of ovarian cancer, promoting the development of POCT technology towards intelligence and precision.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Photonics
·
2022
·Vol. 9
·期 10
·784
·
IF 2.536
Zixin Yang,Gaozhe Cai,Jianlong Zhao,Shilun Feng
Urine examinations are widely applied in hospitals using urine test strip analyzers or other sophisticated professional instruments. However, such methods are inconvenient health monitoring of patients at home. Herein, we construct an optical device for point-of-care testing (POCT) for urine analysis at home or on the spot. A black box and color calibration curve are established to eliminate the influence of ambient light with an independent internal lighting system included in the device. A Raspberry Pi with a CSI camera is programmed to automatically collect the strip images and identify the HSV values of the image with an image processing algorithm. During this process, these corrected colors are converted to concentration values by preloaded standard curves. Under optimal conditions, the proposed POCT device can quantitatively and automatically detect glucose within 1 min, with linear detection ranging from 2 mM to 60 mM and a detection limit of 1.16 mM. In addition, the device demonstrates satisfactory accuracy and quantitative analysis of ketone bodies, glucose, protein, occult blood, pH, and leukocytes in human urine samples with high-resolution concentrations, achieving results similar to those obtained with hospital instruments. The proposed device is portable and user-friendly, providing convenient colorimetric analysis for urine. Furthermore, the proposed device also has considerable potential for the development of in vitro diagnosis methods through combination with other test strips.Keywords:colorimetry detection;HSV parameters;point-of-care testing;urine test
研究领域:
医学诊断
·
生物工程
✓ 已验证
产品关联
DOI
高影响因子
Advanced Science
·
2026
·
IF 14.1
Menghan Li,Kai Su,Ruiwei Hu,Ke Gao,Yuanjie Liu,Lu Zhang,Ningke Fan,Yirong Chen,Wei Cheng,Shijia Ding,Haiping Wu
Electrochemical point‐of‐care testing for complex clinical samples is frequently hampered by matrix interference, signal instability, and laborious electrode modification procedures. Herein, we report a magnetic portable electrochemical immunosensor utilizing a conductive, anti‐fouling hydrogel coating composed of sodium alginate, polyethylene glycol, and g raphene (SPG). Upon calcium‐ion triggering, the SPG solution forms a stable electrode hydrogel coating within 1 min, effectively resisting nonspecific adsorption while maintaining low‑impedance electron transfer. Guided by a 3D‐printed magnetic apparatus, the MpECis directly captures target‐bound magnetic complexes, enabling rapid enrichment and electrical readout within 25 min. By implementing time‑domain averaging over the enzymatic reaction plateau, the coefficient of variation of electrical signals generated by nanoenzyme catalysis was reduced to 3.4%, markedly improving measurement reproducibility. The clinical practicality of the SPG MpECis platform was validated through three independent cohorts, including detection of cardiac troponin I in 59 serum samples, quantification of extracellular vesicles in 47 plasma samples, and detection of C‐reactive protein in 17 fingertip blood samples. Compared with the reference method, the area under the curve of this method are 0.99, 0.93, and 1.00, respectively. This platform offers a practical route for stable electrochemical diagnostics in complex biological matrices and home‐based precision medicine. The SPG‐MpECis electrochemical POCT platform developed in this study enables direct detection of biomarkers in complex biological samples such as serum and fingerstick blood, demonstrating high concordance with results from clinical reference methods and holding broad potential for at‐home testing applications.
✓ 已验证
DOI
PubMed
高影响因子
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