The development of mRNA delivery carriers with innate immune stimulation functions has emerged as a focal point in the field of mRNA vaccines. Nonetheless, the expression of mRNA in specific sites and innate immune stimulation at specific sites are prerequisites for ensuring the safety of mRNA vaccines. Based on the synthetic PEIRs carriers library, this study identifies an innovative mRNA delivery carrier named POctS with the following characteristics: 1) simultaneously possessing high mRNA delivery efficiency and stimulator of interferon genes (STING) stimulation function. 2) Leveraging the distinctive site-specific delivery capabilities of POctS, the expression of mRNA at specific sites and the activation of innate immune responses at designated sites are achieved, minimizing formulation toxicity and maximizing the vaccine performance. 3) Tailoring two types of mRNA vaccines based on POctS according to the immune infiltration status of different types of tumors. Briefly, POctS-loading ovalbumin (OVA) mRNA as a tumor antigen vaccine achieves the prevention and treatment of melanoma in mice. Further, POctS-loading mixed lineage kinase domain-like protein (MLKL) mRNA as an in situ tumor vaccine effectively treats orthotopic pancreatic cancer in mice. This delivery carrier offers a feasible mRNA vaccine-based immunotherapy strategy for various types of tumors.
Herein, a robust instrument-free point-of-care tests (POCTs) strategy was proposed for rapid naked-eye detection of artificial food colorants (AFCs) using filter paper as a sampling and response device. Indicator papers were prepared by one-step polycondensation of silane coupling agents onto glass microfiber filter papers, then colorimetric cards with fine color mappings to AFCs concentration gradients were handily fabricated by AFCs extraction with indicator papers via electrostatic interaction and hydrophobic effect. The lowest concentration for AFCs visual detection was as low as 10 −6 M and the whole POCTs process could be finished within 1 min. Furtherly, color information (RGB and gray-scale values) of AFCs-stained indicator papers and colorimetric cards from smartphone APP was used to reconstruct digitized colorimetry and reinforce its validity by mitigating the possible errors from subjective judgement on colors. Finally, such a dual-mode POCTs strategy was experimentally proved to be highly reliable for rapid visual detection of three AFCs (brilliant blue, Allura red, and sunset yellow) in real drinks by contrast with several instrumental methods like spectrophotometry, HPLC and MS. Due to its fine portability, speediness, low cost and user-friendliness, this strategy paves a promising way for AFCs-related food safety control.
危险品化学品经营许可证（带存储）