查找使用阿拉丁产品的科研文献

PP2C phosphatases Ptc1 and Ptc2 dephosphorylate PGK1 to regulate autophagy and aflatoxin synthesis in the pathogenic fungus Aspergillus flavus

Zhuo Zhu,Mingkun Yang,Guang Yang,Bei Zhang,Xiaohong Cao,Jun Yuan,Feng Ge,Shihua Wang

Ethylene-mediated CsWRKY41 regulates the PYL-PP2C module to promote adventitious root formation in cucumber under waterlogging stress

Hu Qiming,Jing Boyan,Bian Yuanyuan,Yang Zhuyuan,Yang Xiaodong,Chen Xuehao,Qi Xiaohua,Xu Xuewen

Waterlogging stress significantly compromises crop growth and yield. In cucumber (Cucumis sativus L.), the formation of adventitious roots (ARs) is a critical morphological adaptation for survival. While ethylene is known to promote AR formation under waterlogging, the interplay between ethylene and abscisic acid (ABA) signaling remains complex. In this study, we modulated ethylene levels using the precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and the inhibitor aminoethoxyvinylglycine (AOA) to investigate the underlying transcriptional networks. RNA-seq analysis of hypocotyls under waterlogging (WL), WL+ACC, and WL+AOA conditions identified a set of ethylene-responsive genes, including the transcription factor CsWRKY41. Functional characterization revealed that CRISPR/Cas9-mediated mutation of CsWRKY41 significantly impaired AR formation, whereas its overexpression enhanced it. Mechanistically, CsWRKY41 directly binds to W-box elements within the promoters of the ABA receptor gene CsPYL4 and the phosphatase gene CsPP2C24, acting as a transcriptional activator of CsPYL4 and a repressor of CsPP2C24. Furthermore, CsPYL4 physically interacts with CsPP2C24 to modulate ABA signaling. Overexpression of CsPYL4 was found to upregulate the peroxidase gene CsPrx2 and increase H2O2 accumulation, thereby facilitating AR development. Collectively, our findings elucidate a novel molecular mechanism wherein ethylene orchestrates AR formation via the CsWRKY41-CsPYL4-CsPP2C24 module to mitigate waterlogging stress.

Transcriptome, degradome and physiological analysis provide new insights into the mechanism of inhibition of litchi fruit senescence by melatonin

Zhengke Zhang,Jialiang Liu,Donald J. Huber,Hongxia Qu,Ze Yun,Taotao Li,Yueming Jiang

Litchi fruit has high commercial value on the international market, but senesces rapidly after harvest. We used weighted gene co-expression network analysis (WGCNA) and degradome technology to investigate the molecular mechanisms of melatonin-mediated delay of litchi fruit senescence through application of exogenous melatonin and p-chlorophenylalanine (p-CPA, an inhibitor of melatonin biosynthesis) treatments. Results demonstrated that exogenous melatonin treatment delayed litchi fruit senescence while p-CPA accelerated senescence. Coupled analyses of transcriptome and physiological parameters of litchi fruit provided the correlation of network modules with dynamic changes in browning index during storage. Additionally, we found that microRNAs (miR858 and miR160a) and their targets were actively involved in melatonin-mediated delay of litchi fruit senescence. Melatonin treatment decreased abscisic acid (ABA) content but increased PP2C and F-box expression levels, suggesting the involvement of ABA signaling in melatonin-mediated antisenescence. The transcriptions of ZAT, NAC and DREB1 were activated by melatonin treatment. Moreover, the major functional genes involved in histone methylation, γ-aminobutyric acid (GABA) metabolism, energy production, reactive oxygen species (ROS) accumulation and cell death were identified in the melatonin-inhibited litchi pericarp browning. Taken together, we first constructed the global map of the important regulators and pathways to delay litchi senescence and pericarp browning mediated by melatonin.