Journal of Materials Chemistry B
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2026
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IF 6.2
Fugang Li,Qiang Li,Feng Chen,Chenxu Yan,Yongxi Zhao,Zhiqian Guo
High sensitivity and accuracy in detecting the myocardial damage biomarker, human cardiac troponin T (cTnT), is critical for the early diagnosis of acute myocardial infarction. However, conventional chemiluminescent (CL) probes suffer from instability, poor solubility, and inadequate bioavailability, which limit their clinical utility. Here, we describe a novel nanosized CL system (lucigenin@PS-COOH) for cTnT detection, wherein carboxyl polystyrene (PS-COOH) microspheres encapsulate lucigenin, significantly enhancing stability, water dispersibility, and antibody conjugation. Compared with traditional CL probes, lucigenin@PS-COOH exhibits exceptional stability, biocompatibility, and CL signal intensity. In addition, lucigenin@PS-COOH demonstrates excellent linearity with clinical data from Longhua Hospital, suggesting strong potential as a clinical assay for cTnT detection in real-world patient samples. Thus, lucigenin@PS-COOH can serve as a powerful tool for accurate cTnT detection in clinical settings and facilitate further research into the role of cTnT in disease.
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2023
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IF 9.8
Xiaoyan Tang,Manjia Chen,Muzi Li,Huanping Liu,Hao Tang,Yang Yang
Micro(nano)plastics are ubiquitous in the environment. Among the microplastics, imidacloprid (IMI) concentration has been increasing in some intensive agricultural regions, thus receiving increased attention. However, only a few studies have investigated the interaction of nanoplastics (polystyrene (PS)) and IMI in vegetable crops. We studied the effects of positively (PS-NH 2 ) and negatively (PS-COOH) charged nanoplastics on the uptake, translocation, and degradation of IMI in Chinese flowering cabbage grown in Hoagland solution for 28 days. PS-NH 2 co-exposure with IMI inhibited plant growth, resulting in decreased plant weight, height, and root length. Translocation of IMI from the roots to the shoots was significantly lower in the presence of PS-NH 2 , whereas PS-COOH accelerated the accumulation and translocation of IMI in plants, thus potentially affecting IMI metabolism in plants. Notably, IMI-NTG and 5-OH-IMI were the two dominant metabolites. PS-NH 2 co-exposure with IMI induced significant oxidation stress and considerably affected the activities of superoxide dismutase (SOD) and peroxidase (POD), indicating that the antioxidant defense system was the main mechanism for reducing oxidative damage. Notably, both positively and negatively charged nanoplastics can accumulate in Chinese flowering cabbage. Plants in the PS-COOH alone treatment group had the highest concentration of nanoplastics in both roots and shoots. The accumulation of nanoplastics, IMI, and its metabolites in plants raises concerns about their combined potential toxicity because it compromises food safety.
研究领域:
环境科学
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农业
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植物科学
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毒理学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
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2023
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IF 8.9
Yu Xing,Jing Li,Jingjing Yang,Junyi Li,Weiyi Pang,Francis L. Martin,Li Xu
Humans are routinely exposed to nanoplastics (NPs) in various ways, and this exposure presents a significant health risk. Nevertheless, there remain gaps in our knowledge, particularly in the mechanisms of toxicity of NPs with different surface charges at very low environmental concentrations. Herein, a spectrochemical approach was used to profile the cytotoxicity of NPs with different surface charges in HepG 2 cells. It was found that all three NPs can cause some biomolecular alterations in cells, affecting cellular lipids, proteins, amino acids, and genetic material. Of these, PS and PS-COOH led to a non-linear dose-response, which may be related to a biphasic dose-response, whereas PS-NH 2 led to a linear dose-response with a gradual increase in toxicity with increasing exposure concentration. In addition, the spectroscopic results showed that surface modifications led to cellular biochemical changes and caused adverse biological effects, with PS-NH 2 exhibiting higher toxicity compared to PS or PS-COOH along with an inhibition of cell proliferation. Surprisingly PS-COOH, although considered the least toxic NP, appears to cause DNA damage. Overall, the toxic effects of different surface-modified NPs in cells were detected for the first time by applying spectrochemical techniques, and these findings provide important data towards understanding the emerging widespread environmental pollution of NPs and their effects on humans.
研究领域:
毒理学
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细胞生物学
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环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）