NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 43
·期 3
·1424-1430
·
IF 3.201
Jun-Tao Cao,Wen-Sheng Zhang,Hui Wang,Shu-Hui Ma,Yan-Ming Liu
A novel fluorescence (FL) immunosensor for prostate-specific antigen (PSA) detection was developed based on the Förster resonance energy transfer (FRET) between nitrogen and sulfur co-doped carbon dot functionalized silica nanospheres (Si/NS-CDs) and Au@Ag NPs. The strategy utilized the PSA primary antibody labeled luminescent Si/NS-CDs as donors and PSA secondary antibody labeled Au@Ag NPs as acceptors. In the presence of PSA, the bio-affinity of the antigen and antibody caused the Si/NS-CDs and Au@Ag NPs to approach at a close enough distance for the FL intensity of the Si/NS-CDs to decline owing to the FRET effect. By virtue of the excellent optical properties of the Si/NS-CDs nanoprobe, the quantitative analysis of PSA has been successfully realized using both FL spectroscopy and a naked-eye readout. The fluorometric results present the desirable analytical performance for PSA detection in the range from 5.0 pg mL−1 to 10 ng mL−1 with a detection limit of 0.95 pg mL−1 (S/N = 3). Moreover, satisfactory results for the determination of the PSA target in samples of human serum were obtained, showing the great promise of the method for application in biochemical analysis.
研究领域:
生物化学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Jianguo Zhang,Shaozhen Wang,Ni Gao,Dexiang Feng,Lun Wang,Hongqi Chen
A new turn-on luminescence energy transfer (LET) system has been designed for the detection of prostate specific antigen (PSA, a cancer marker) that utilizes Mn 2+ -enhanced long wavelength luminescence NaYF 4 : Yb, Er upconversion nanorods as the donor and gold nanorods as the acceptor. The Mn 2+ -doped NaYF 4 :Yb,Er upconversion luminescence nanorods with an emission peak located in the red region were synthesized. The presence of Mn 2+ markedly increased the luminescence intensity over that of the NaYF 4 :Yb, Er upconversion nanomaterials (excited by a 980 nm continuous wavelength laser). The surfaces of Mn 2+ -doped NaYF 4 :Yb, Er upconversion nanorods were modified with poly(acrylic acid). Antibodies against prostate specific antigen were bound to the surface of the carboxyl-functionalized upconversion nanorods, which acted as the energy donor in this LET system. Gold nanorods with an absorption band at ~666 nm were synthesized by the seed growth method, acted as the energy acceptor. The emission band of the upconversion nanorods overlapped well with the absorption band of the gold nanorods. The luminescence was quenched because of the electrostatic interactions that shortened the distance between the donor (negatively charged) and the accepter (positively charged).When the PSA antigen was added into the system, the energy acceptor and the energy donors were separated because the binding affinity between PSA and anti-PSA was greater than the electrostatic interactions, and thereby the luminescence was recovered. The linear range of detecting PSA was from 0.1172 to 18.75 ng/mL ( R =0.995), with a limit of detection for PSA as low as 0.1129 ng/mL. The method was successfully applied to the sensing of PSA in human serum samples.
研究领域:
生物技术
·
纳米技术
·
分析化学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 6
·1-9
·
IF 6.232
Li Xiaomeng,Kong Weisu,Qin Xia,Qu Fengli,Lu Limin
A facile and sensitive self-powered cathodic photoelectrochemical (PEC) aptasensor is reported for the detection of prostate-specific antigen (PSA) based on CuO-Cu 2 O nanowire array grown on Cu mesh (CuO-Cu 2 O NWA/CM) as electrode. The mixed narrow band gaps of the CuO-Cu 2 O heterostructure ensured its wide absorption band, effective electron/hole separation, and high photocatalytic activity in the visible region. In addition, nanowires directly grown on the substrate provided high specific surface area and exposed abundant active sites, thus guaranteeing its high photocatalytic efficiency. Therefore, the self-powered sensor exhibited favorable analytical performance with fast response, wide linear ranges of 0.01 to 5 ng/mL and 5 to 100 ng/mL, an acceptable detection limit of 3 pg/mL, and reasonable selectivity and stability. The proposed CuO-Cu 2 O NWA/CM can be considered a promising visible light-responsive photoactive material for fabrication of PEC aptasensor with high performance.
a Schematic illustration of construction process of PEC sensing platform based on the CuO-Cu 2 O composite for PSA detection. b Schematic mechanism of the operating PEC system.
研究领域:
分析化学
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生物传感器
·
纳米材料
·
癌症诊断
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2022
·Vol. 24
·期 5
·1-10
·
IF 2.533
Chen Junjie,Li Xiangqian,Yu Xiaoqi,Zhou Wei,Wang Qianming
Two-dimensional platforms generate considerable interests due to diverse application. We report a “turn-on” fluorescent nanoprobe for prostate-specific antigen (PSA) detection. It is based on cobalt oxyhydroxide nanosheets (CoOOH NSs). Fluorescein amidite (abbreviated as FAM)-labeled aptamer probe (abbreviated as FAM-aptamer) has been adsorbed onto the nanosheets. Energy transfer between substrate and optical species has induced the quenching of FAM-aptamer emission. The strong affinity of FAM-aptamer to the target PSA causes the formation of a rigid aptamer structure. The integration between FAM-aptamer and CoOOH NSs has been drastically affected. The release of the aptamer probe from the nanosheet surface has been realized. The green luminescence has been recovered. The dynamic nanosensor exhibits highly sensitive performance toward PSA with a linear detection range from 0.1 to 5 ng/mL. Its detection limit is measured to be 56.1 pg/mL. Therefore, a simple and efficient sensing platform for the detection of prostate cancer can be established.
研究领域:
生物纳米技术
·
分析化学
·
癌症诊断
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yuan Zhao,Han Liu,Lixia Shi,Wangwang Zheng,Xiaohui Jing
Early detection of prostate specific antigen (PSA) could reduce the mortality from prostate cancer. Electroactive nanostructures have attracted much attention due to their excellent electronic conductivity and favorable biological microenvironment. In particular, electroactive Cu 2 O nanoparticles (NPs) and Ag NPs exhibited unique oxidation signals at around -0.1 V and +0.2 V. The separated and non-interfere oxidation signals enabled them to be alternative electrochemical beacons for the establishment of ratiometric electrochemical sensor for PSA detection. Electroactive Cu 2 O NPs were designed to grow on the surface of reduced graphene oxide/Fe 3 O 4 nanosheets (rGO/Fe 3 O 4 NSs) and served as reference tags due to the stable differential pulse voltammetry (DPV) signals of Cu 2 O NPs, magnetic properties for the absorption on electrode and the accelerated electron transfer ability of rGO. In order to improve the sensitivity of detection, a large number of small electroactive Ag nanodots were dispersed on the surface of [email protected] (RF) NPs and served as detection tags. Electroactive [email protected] NPs were assembled with rGO/Fe 3 O 4 /Cu 2 O NSs for the fabrication of ratiometric electrochemical aptasensor. With the addition of PSA, aptamers modified [email protected] NPs disassembled from electrode surface, resulting in the decrease of DPV signals near +0.2 V and unchanged DPV signals at -0.1 V. This developed ratiometric electrochemical aptasensor achieved sensitive and accurate PSA detection. This proposed ratiometric electrochemical aptasensor has the advantages of easy preparation, simple structure and wide detection ranges.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
癌症研究
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 46
·10662-10669
·
IF 5.344
Sixuan Wu,Caihong Wang,Jinhong Wang,Hongliang Tan
Coordination polymers (CPs) have been extensively investigated for a variety of applications because of their tunable structures and properties. In this work, we demonstrated the potential of catalytic CPs in the fabrication of an integrated multifunctional composite for establishing a cascade amplified immunoassay. For this purpose, an Fe(III)-based CP (FeCP) with peroxidase-like activity was employed as a model of catalytic CPs to simultaneously integrate glucose oxidase (GOx) and the anti-prostate specific antigen (anti-PSA) antibody through a self-adaptive inclusion process. This leads to the formation of a dual-functional anti-PSA/GOx@FeCP composite with cascade catalytic activity and capture ability to target the antigen. Benefiting from the shielding effect of FeCPs as a host, a significantly improved stability against harsh environments can be achieved for the loaded GOx and anti-PSA antibody in the composite. On this basis, by utilizing anti-PSA/GOx@FeCPs as a detection antibody, a colorimetric immunoassay based on the cascade catalysis of GOx and FeCPs as a signal amplified enhancer was developed for the detection of PSA. Under optimal conditions, satisfactory detection results have been achieved in both buffered aqueous solutions and serum samples. We believe that this study will open up a new avenue for the rational design and fabrication of multifunctional composites while offering a new cascade amplification strategy for PSA detection.
研究领域:
生物化学
·
分析化学
·
材料科学
·
免疫分析技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Wenwei Pan,Ziyu Han,Ye Chang,Xuexin Duan
We have developed a new three-dimensional (3D) surface for use in biosensors that is based on modified novel thorns-like polyelectrolytes (3D-PETx), which comprises of poly- L -lysine (PLL) appended with multitude oligo (ethylene glycol) (OEG) and biotin moieties. It tethered to the sensor surface by PLL, while the OEG-biotin chains are forced to stretch away from the surface for target detections. Due to its 3D structure, the number of the OEG-biotin per surface unit is markedly increased compared to conventional 2D polyelectrolytes (2D-PET) coating. Their antifouling property and sensing performance for human IgG and PSA were compared with the 2D-PET by BioLayer Interferometry (Blitz), Surface Plasmon Resonance (SPR), microfluidic devices and Enzyme-Linked ImmunoSorbent Assay (ELISA). Experimental results show that 3D-PETx presents higher sensitivity for biomarker detection both in buffer and in serum and provides an almost non-fouling surface even in undiluted serum. In addition, a sensitive PSA detection was achieved in undiluted serum with a LOD down to 0.6 ng/mL. The successful immunosensing in undiluted serum demonstrate the potential of the 3D-PETx coating for real clinical applications.
研究领域:
生物传感器
·
聚合物化学
·
分析化学
·
生物技术
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）