MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Mengmeng Zhang,Ximei Hu,Lisha Mei,Li Zhang,Xiao Wang,Xiaochen Liao,Xiuwen Qiao,Chenglin Hong
Conventional electrochemical immunosensors use electroactive materials often suffer from leakage of electroactive materials and signal instability. To solve this problem, this paper proposes an electrochemical immunosensor based on MOF-235 adsorption aggregation signal amplification strategy for the sensitive detection of PSA. Methylene blue (MB) is a cationic dye with redox properties. It has electrochemical reversibility, high electron transfer rate and good biocompatibility. Therefore, it is often used as an electroactive substance in electrochemical immunosensors. However, the non-specificity and poor stability of MB will inevitably distort the diagnostic results and limit its application. In this experiment, MOF-235, a fluffy metal porous MOF material with two carboxyl groups has been prepared and it has a large specific surface area and good biocompatibility. Meanwhile, it can generate electrostatic attraction with the amino groups on MB, and firmly fix methylene blue on the MOF material, which can effectively prevent the signal leaks of MB during the test, and after being adsorbed, the reunion of MB can be controlled, which is conducive to electron transmission thus amplifying the electrical signal. The produced electrochemical immunosensor has good stability and repeatability, and the concentration of the analyte is directly linear with the magnitude of the electrical signal. Under optimum conditions, the proposed immunosensor shows a wide detection range from 10 pg·mL −1 to 1200 pg·mL −1 with a low detection limit of 3 pg·mL −1 .
研究领域:
生物医学工程
·
分析化学
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产品关联
DOI
高影响因子
CHROMATOGRAPHIA
·
2017
·Vol. 80
·期 3
·463-471
·
IF 1.402
Xu Mei,Chen Kun,Luo Chen,Song Guoxin,Hu Yaoming,Cheng Hefa
Abstract In this paper, a simple but efficient method for the extraction and analysis of bifenthrin in water samples was proposed. The Fe 3 O 4 -based nanocomposites (Fe 3 O 4 @m-SiO 2 /PSA@Zr-MOF) were successfully synthesized and developed as adsorbents for magnetic solid-phase extraction. With the solvothermal reaction and sol–gel method, the resultant adsorbents exhibited excellent characteristics of large surface areas, homogeneous pore size, strong magnetic responsivity and high hydrophilicity. The parameters affecting the extraction performance including adsorbent amounts, adsorption time, species of eluents and desorption time were investigated. Under the optimal conditions, validation experiments such as recovery, reproducibility and limits of detection (LOD) were carried out and showed satisfactory results. The LOD of the targeted analytes was found to be 0.5 μg L −1 . The average recovery was 103.2% with the intra-day and inter-day relative standard deviations of 2.0 and 3.5%, respectively. Ultimately, the novel magnetic adsorbents were successfully applied to detect the bifenthrin compound in real water samples. Graphical Abstract
研究领域:
环境科学
·
分析化学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2020
·
IF 9.038
Xue Yang,Yi Pu,Yifei Zhang,Xiaofang Liu,Jianxin Li,Ding Yuan,Xin Ning
In this study, a new barium titanate@polyurethane/polysulfonamide (BaTiO 3 @PU/PSA) composite nanofibrous membrane with comprehensive properties for high temperature filtration and robust PM2.5 removal was successfully fabricated through the blending spinning of PU and PSA and the introduction of BaTiO 3 . As a consequence, the BaTiO 3 @PU/PSA membrane achieved the high capture efficiency of 99.99 % for fine particulates, low pressure drop of 39.4 ± 0.2 Pa, good mechanical property (13.27 MPa), sufficient flexibility, high thermal stability (up to 300 °C), favorable flame-retardancy as well as superior chemical resistance against acid and alkali. Especially, to intuitively reveal the relationship between the fiber structure, high temperature environment, gas velocity and filtration performance of the composite membrane, the filtration processes were carefully investigated through the analog simulation. More importantly, the BaTiO 3 @PU/PSA membrane exhibited high-efficiency PM2.5 purification capacity, and the removal efficiency kept stable after high temperature, acid or alkali treatment, ascribing to the advantageous structure of PSA, PU and BaTiO 3 . Overall, the BaTiO 3 @PU/PSA nanofiber membranes with versatility are a promising high-efficiency candidate for dust removal, particularly in harsh conditions.
研究领域:
材料科学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
Applied Surface Science Advances
·
2023
·
IF 6.2
Zhufeng Shao,Yonglong Zhang,Jianyong Cheng,Libin Shi,Xiujuan Yang,Xiaoming Xiu
Firstly, oxygen vacancy defects are intentionally introduced in the preparation process of TiO 2 nanotube arrays (TiO 2 -NTAs) and porous silicon arrays (PSA) films through the anodic oxidation method after annealing treatment, as expected. Then, CdS/TiO 2 -NTAs and CdS/PSA heterostructures are successfully fabricated using the electrodeposition method, incorporating abundant surface vacancy defects. The crafted CdS/TiO 2 -NTAs nanoheterojunctions presented elevated photoelectrochemical (PEC) performances and sensing activities compared with that of CdS/PSA, validating by photodegradation and PEC biosensing tests, which mainly ascribed to the synergistic effect between the promoted carriers separation efficiency and boosted surface active sites induced by vacancies defects. Furthermore, a comparative and comprehensive interfacial charge transfer mechanism and qualitative dynamic processes for CdS/TiO 2 -NTAs and CdS/PSA type-II nanoheterojunctions are proposed, revealing an enlarged staggered band offset between CdS and TiO 2 -NTAs. The evaluation of this band offset was conducted using nanosecond time-resolved transient photoluminescence and steady photoluminescence spectra, respectively. Following this scheme, the photodegradation rate for CdS/TiO 2 -NTAs nanocomplex toward degradation of MO was 92.8% and 1.06 times than that of CdS/PSA; while the PEC biosensing performance for CdS/TiO 2 -NTAs showed a detection range 0 – 600 μM and limit of detection of 2.3 μM, with a sensitivity of 456 mA cm −2 M −1 .
研究领域:
材料科学
·
环境科学
·
分析化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Zhixin Lei,Longfukang Yao,Yuqing Tan,Zhiqun Lei,Taolei Sun
The overexpression of virulence factors and the induced inflammatory cytokine storm, resulting in tissue damage, represent significant challenges in treating antibiotic-resistant bacterial infections with conventional antibiotics. Herein, we have developed a bifunctional nanoparticle loaded with antibiotics (PSA@PLGA-TD/SMT) designed for precise response to the virulence proteins of drug-resistant bacteria while protecting the host from excessive inflammatory damage. This is achieved by modifying polylactic- co -glycolic acid (PLGA) nanoparticles with polysialic acid (PSA). Tildipirosin (TD), through hydrophobic interactions, is encapsulated within the core, while S-Methylisothiourea (SMT) is electrostatically adsorbed onto the shell layer. In vitro, the polysialic acid in the nanoparticles interacts with the neuraminidase overexpressed on the surface of Glaesserella parasuis (Gps), triggering disintegration and subsequent release of TD, which effectively kills the bacteria. Additionally, SMT functions to suppress the secretion of inducible nitric oxide synthase (NOS2), promoting the phenotypic transformation of macrophages and reducing the expression of pro-inflammatory factors. Ultimately, PSA@PLGA-TD/SMT achieves an extended drug circulation time through its prolonged retention effect, effectively alleviating discomfort caused by resistant bacteria. Therefore, the construction of nanocarriers for precise targeting of drug-resistant pathogens, delivering antibiotics, and preventing host-induced inflammatory damage presents an effective treatment strategy. This approach not only addresses the direct issue of bacterial eradication but also mitigates the collateral damage typically associated with severe infections, offering a more comprehensive and nuanced approach to managing drug-resistant bacterial infections.
研究领域:
生物医学工程
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微生物学
·
药理学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Jianguo Zhang,Shaozhen Wang,Ni Gao,Dexiang Feng,Lun Wang,Hongqi Chen
A new turn-on luminescence energy transfer (LET) system has been designed for the detection of prostate specific antigen (PSA, a cancer marker) that utilizes Mn 2+ -enhanced long wavelength luminescence NaYF 4 : Yb, Er upconversion nanorods as the donor and gold nanorods as the acceptor. The Mn 2+ -doped NaYF 4 :Yb,Er upconversion luminescence nanorods with an emission peak located in the red region were synthesized. The presence of Mn 2+ markedly increased the luminescence intensity over that of the NaYF 4 :Yb, Er upconversion nanomaterials (excited by a 980 nm continuous wavelength laser). The surfaces of Mn 2+ -doped NaYF 4 :Yb, Er upconversion nanorods were modified with poly(acrylic acid). Antibodies against prostate specific antigen were bound to the surface of the carboxyl-functionalized upconversion nanorods, which acted as the energy donor in this LET system. Gold nanorods with an absorption band at ~666 nm were synthesized by the seed growth method, acted as the energy acceptor. The emission band of the upconversion nanorods overlapped well with the absorption band of the gold nanorods. The luminescence was quenched because of the electrostatic interactions that shortened the distance between the donor (negatively charged) and the accepter (positively charged).When the PSA antigen was added into the system, the energy acceptor and the energy donors were separated because the binding affinity between PSA and anti-PSA was greater than the electrostatic interactions, and thereby the luminescence was recovered. The linear range of detecting PSA was from 0.1172 to 18.75 ng/mL ( R =0.995), with a limit of detection for PSA as low as 0.1129 ng/mL. The method was successfully applied to the sensing of PSA in human serum samples.
研究领域:
生物技术
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纳米技术
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分析化学
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Qin Lei,Yu Wang,Wanxin Dong,Haitao Sun,Jiaxin Lv,He Li
Herein, we used Prussian blue (PB) as an indicator for self-powered electrochromic visual sensing driven by photoelectrochemical (PEC) cell to detect prostate specific antigen (PSA). The sensor consists of a photoanode and a PB/ITO cathode. Under the visible light excitation, photoelectrons generated from the photoanode will transmit to PB through an external circuit, making the blue PB reduced to transparent Prussian white (PW). In the presence of PSA, the transfer rates of photoelectrons slow due to steric hindrance , leading to different fading degree of PB. Moreover, the color intensity of PB is proportional to the concentration of PSA, we can quantitatively detect the PSA according to the color intensity. Under the optimal conditions, the linear range of detecting PSA was 0.1–50 ng/mL with the detection limit of 0.03 ng/mL (S/N = 3). Meanwhile, the prepared novel sensing platform exhibited great selectivity and provided a rapid, visual method for PSA detection in human serum, which has great application prospects in point-of-care (POC) diagnostics.
研究领域:
分析化学
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生物传感器
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医学诊断
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Bo Zhang,Weichen Gao,Jiafang Piao,Yunjie Xiao,Bin Wang,Weipan Peng,Xiaoqun Gong,Zefang Wang,Haitao Yang,Jin Chang
Nowadays, increasing analytical sensitivity is still a big challenge in constructing membrane-based fluorescence immunochromatography test strips (FICTS). However, the bioactivity of antibody (Ab) immobilized on the test line (T line) of porous nitrocellulose membrane (PNM), which directly influences the analytical sensitivity, is less studied. In this work, a novel amphiphilic hydrophobin (HFBI) protein was introduced to modify the T line to effectively retain the Abs’ bioactivity. The results indicated that HFBI could self-assemble on the PNM and immobilize the Abs in the “stand-up” orientation. Compared with the conventional FICTS, the HFBI-modified FICTS with only 0.2 mg/mL of monoclonal Abs on T line enable more accurate quantitative detection and better sensitivity (0.06 ng/mL for prostate specific antigen), which is more than 2 orders of magnitude lower than that of the conventional FICTS with the same concentration of monoclonal Abs on T line. Furthermore, the accuracy of this HFBI-modified FICTS was investigated by testing 150 clinical serum samples and the detection results were coincident with those by electrochemiluminescence immunoassay. Our results provide a novel and promising strategy of Ab immobilization on FICTS for near-patient and point-of-care application.
研究领域:
生物技术
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分析化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 14
·2421
·
IF 5.1
Mengqiong Yang,Guangming Mei,Daoxiang Huang,Xiaojun Zhang,Pengfei He,Si Chen
A sensitive method was developed for detecting diazepam residues in aquatic products using magnetic dispersive solid-phase extraction (MDSPE) coupled with ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS). Samples extracted with 1% ammonia–acetonitrile were purified using synthesized Fe3O4@SiO2-PSA nanoparticles via MDSPE before UPLC-MS/MS analysis. Separation was performed on a C18column with gradient elution using 0.1% formic acid–2 mM ammonium acetate/methanol. Detection employed positive electrospray ionization (ESI+) in multiple reaction monitoring (MRM) mode. Characterization confirmed Fe3O4@SiO2-PSA’s mesoporous structure with excellent adsorption capacity and magnetic properties. The method showed good linearity (0.1–10 μg/L,r> 0.99) with an LOD and LOQ of 0.20 μg/kg and 0.50 μg/kg, respectively. Recoveries at 0.5–15.0 µg/kg spiking levels were 74.9–109% (RSDs 1.24–11.6%). This approach provides rapid, accurate, and high-precision analysis of diazepam in aquatic products, meeting regulatory requirements.
研究领域:
分析化学
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食品安全
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材料科学
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产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2021
·Vol. 33
·期 40
·2103999
·
IF 30.849
Binshuai Wang,Shudong Zhang,Jingxin Meng,Li Min,Jing Luo,Zhongpeng Zhu,Han Bao,Ruhua Zang,Shaohui Deng,Fan Zhang,Lulin Ma,Shutao Wang
The prostate-specific antigen (PSA) has been widely used for the early diagnosis of prostate cancer during routine check-ups. However, the low sensitivity of regular PSA tests in the PSA gray zone often means that patients are required to undergo further invasive needle biopsy for the diagnosis of prostate cancer, which may lead to potential overdiagnosis and overtreatment. In this study, a circulating tumor cell (CTC)-chip based on an evaporation-induced reduced graphene oxide (rGO) coating is presented, which enables a highly specific and non-invasive diagnosis of prostate cancer in the PSA gray zone. During the evaporation process of the rGO dispersion, the Marangoni effect induces the self-assembly of a hierarchical micro/nanowrinkled rGO coating, which can capture CTCs after subsequent surface modification of capture agents. Compared to the low diagnostic sensitivity (58.3%) of regular PSA tests, a combination of CTC detection and PSA-based hematological tests via machine-learning analysis can greatly upgrade the diagnostic sensitivity of this disease to 91.7% in clinical trial. Therefore, this study provides a non-invasive alternative with high sensitivity for the diagnosis of prostate cancer in the PSA gray zone.
研究领域:
肿瘤学
·
生物医学工程
·
纳米技术
·
医学诊断
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DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Yuanyuan Du,Hongmei Liu,Ying Chen,Yiran Tian,Xiaoling Zhang,Chenjie Gu,Tao Jiang,Jun Zhou
In the field of SERS-based immunoassay, the recyclable utilization of noble metal materials is essential for their practical application in clinical medicine. Herein, a kind of novel multifunctional Fe 3 O 4 @TiO 2 @Ag microparticles (MPs) was synthesized through a poly-step reaction, and then their feasibility for recyclable label-free SERS-based immunoassay of prostate specific antigen (PSA) was demonstrated. The photocatalytic efficiency of the Fe 3 O 4 @TiO 2 @Ag MPs was fully adjusted by tuning the thickness of the middle TiO 2 shell and the areal density of the outside Ag NPs. Particularly, the Fe 3 O 4 core played a critical role in further improving the photocatalytic activity under external magnetic field. Thereafter, the successfully photocatalytic degradation and recyclable detection of PSA in human serum was confirmed by the change of O 1s, N 1s, and C 1s signals in the XPS characterization and a limit of detection as low as 16.25 pg/mL was realized. Furthermore, the quantitative recyclable detection of PSA in clinical serum samples with prostate cancer was conducted, and the results are basically consistent with those of the traditional chemiluminescent immunoassay, showing small relative deviation less than or equal to 5.7%.
研究领域:
分析化学
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生物医学工程
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纳米技术
·
临床医学
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产品关联
DOI
高影响因子
ANALYTICAL LETTERS
·
2021
·
IF 2.329
Zheng Chang,Yimeng Xu,Yuting Shen
A novel sandwich-type electrochemical immunosensor was fabricated for the ultrasensitive electrochemical determination of prostate specific antigen (PSA) using silver-functionalized polyethyleneimine (PEI)-silica nanoparticles (NPs) (Ag+/PEI/SiO2 NPs) as electrochemical labels. First, the immunosensor platform was prepared by covalently immobilizing capture antibodies on a poly diallyldimethyl ammonium chloride/carboxylated-gold nanoparticles (PDDA/AuNPs) modified glassy carbon electrode (GCE). After sandwich-type immunoreactions, the detection antibody was labeled by the amino-containing Ag+/PEI/SiO2 NPs by strongly binding to carboxyl groups of protein. Next, many Ag+ with good complexing performance from each PEI/SiO2 nanoparticles released after hydrofluoric acid (HF) in situ etching. Here, the AuNPs acted as a catalyst to reduce Ag+ into metallic Ag using PEI as the reducing agent. Accordingly, an extremely sharp voltammetric peak of electro-oxidized metallic Ag can be determined directly through differential pulse voltammetry (DPV) without metal pre-concentration, and had a close relationship with the concentrations of PSA antigen. Under the optimized conditions, the immunoassay exhibited good sensitivity and selectivity for the determination of PSA with a linear range from 0.01 to 10 pg·mL−1 and a detection limit of 3.5 fg·mL−1. The new strategy provides a reliable, highly sensitive determination of the prostate cancer biomarker in biological samples with great potential for the fabrication of devices for clinical diagnostics.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
生物医学工程
·
临床诊断
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）