A novel porous polymeric microsphere for the selective adsorption and isolation of conalbumin
Porous polymeric microspheres , poly(styrene-divinyl benzene, PSDVB)-poly(ethylene glycol monoallyl ether, PEGMAE), termed as PSDVB-PEGMAE, are prepared via double emulsion interfacial polymerization strategy. PSDVB-PEGMAE microspheres exhibit a mean diameter of 2.98 μm, and possess heterogeneous porous structure with a pore volume of 0.354 cm 3 g −1 and a pore size of 34.3 nm. PEGMAE moiety is identified on the external surface of the microspheres, while both PSDVB and PEGMAE moieties are found in the interior pores. The PSDVB-PEGMAE microspheres possess favorable selectivity towards the adsorption of conalbumin (ConA) through hydrogen-bonding and hydrophobic interactions, via surface and inter-pore adsorption. At pH 6, an adsorption capacity of 171.9 mg g −1 is achieved for ConA. The captured ConA may be readily recovered by stripping with a cetane trimethyl ammonium bromide (CTAB) solution (0.1%, m/v). The microspheres are further used for the isolation of ConA from egg white, deriving high purity ConA as demonstrated by SDS-PAGE assay.
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