Nanoscale
·
2025
·
IF 5.1
Chiyi Wei,Yanlong Li,Haijiao Xu,Molei Hao,Tianxi Wang,Weiyuan Huang,Wanlu Cao,Zihao Li,xiaoming wei,Zhongmin Yang
Chiral recognition plays a vital role in chemical, biological, and pharmaceutical studies. However, differentiating enantiomers is still challenging. This study presents a new approach to enhance the distinction between enantiomers in surface-enhanced Raman scattering (SERS) by leveraging the coupling effect of propagating surface plasmon (PSP) and localized surface plasmons (LSP). We construct a SERS substrate that integrates laser-induced periodic surface structures with chiral plasmonic nanoparticle 432 helicoid III, creating a PSP-LSP coupling mechanism. The results demonstrate that the composite substrate not only enhances the SERS signal but also effectively amplifies the disparity between enantiomers. This advancement paves a new way in chiral SERS substrate design and the research of enantiomeric disparity amplification.
研究领域:
化学
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生物学
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药学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 18
·3438
·
IF 5.2
Chaoqun Sun,Guodong Wang,Jing Sun,Jiyong Yin,Jian Huang,Zizi Li,Di Mu,Menglu He,Tingting Liu,Jiali Cheng,Hanchen Du,Yan Chen,Wenjie Qu
Polygonatum sibiricumPolysaccharide (PsP) with antioxidant function is the main active component ofPolygonatum sibiricum(P.sibiricum). The currently poor extraction yield and extraction methods of PsP cannot meet the application of that in food industrial production. In this research, an ultrasound-assisted extraction-deep eutectic solvents (UAE-DESs) method, which has never been used in the PsP industry, was first used to extract PsP. The extraction conditions were optimized by the response surface method (RSM). Both the extraction yield and antioxidant function were simultaneously considered during the optimization process. The indicators of PsP’s level and antioxidant activity in vitro were used to present the extraction yield of the UAE-DESs method, the purity, and the antioxidant effect of PsP. Under the optimal conditions, which included a liquid–solid ratio of 26:1 (mL:g), extraction temperature of 80 °C, ultrasonic time of 51 min, and ultrasonic power of 82 W, the PsP extraction yield could reach (43.61 ± 0.09)%, which was obviously higher than single DESs (33.81%) and UAE (5.83%), respectively, and the PsP appeared favorably antioxidant function. This research proposed an efficient extraction method for PsP, filled the basic research gap, and further improved the development of PsP as a dietary supplement with antioxidant function in the food industry.Keywords:Polygonatum sibiricumPolysaccharide (PsP);ultrasound-assisted extraction-deep eutectic solvents (UAE-DESS);antioxidant function;response surface method (RSM)
研究领域:
食品科学
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药学
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生物技术
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Zhenguang Liu,Haiyu Ni,Lin Yu,Shuwen Xu,Ruonan Bo,Tianxin Qiu,Pengfei Gu,TianYu Zhu,Jin He,Adelijiang Wusiman,Shaowu Zhu,Jiaguo Liu,Yuanliang Hu,Deyun Wang
Recently, the cubosomes have been widely studied as drug carriers. It has been described that cubosomes could further stimulate the immune response after carrying the immune enhancer. Polygonatum sibiricum polysaccharide (PSP), one of the most important biologically active ingredients of Polygonatum sibiricum , has been reported as an immunostimulant to improve immune responses. This study was aimed to observe the immunomodulation effects of ovalbumin (OVA) absorbed cetyltrimethylammonium bromide-modified Polygonatum sibiricum polysaccharide cubosomes (CTAB-modified PSP-Cubs/OVA). Firstly, the antigen uptake of CTAB-modified PSP-Cubs/OVA by macrophages was determined in vitro. After that, mice were immunized with CTAB-modified PSP-Cubs/OVA. The activation of dendritic cells in lymph nodes, activation of lymphocyte, ratios of CD4 + to CD8 + , the concentrations of OVA-specific IgG in serum and the cytokines concentrations were analyzed. As the results showed, CTAB-modified PSP-Cubs/OVA could promote the production of OVA-specific IgG in serum. The ratio of CD4 + to CD8 + in CTAB-modified PSP-Cubs/OVA group was significantly increased compared with other groups. CTAB-modified PSP-Cubs/OVA could significantly activate dendritic cells and promote lymphocyte proliferation. The results indicated that CTAB-modified PSP-Cubs/OVA could promote the secretion of related cytokines and the proliferation of lymphocytes, stimulate the cellular immune response and increase the level of humoral immunity. Above all, CTAB-modified PSP-Cubs had good adjuvant activity.
研究领域:
免疫学
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药剂学
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生物技术
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2019
·
IF 4.213
Haiyu Ni,Shuwen Xu,Pengfei Gu,Adelijiang wusiman,Yue Zhang,Tianxin Qiu,Zhenguang Liu,Han Ni,Yuanliang Hu,Jiaguo Liu,Yi Wu,Deyun Wang
This study aimed to optimize the preparation conditions for cetyltrimethylammonium bromide-modified Polygonatum sibiricum polysaccharide cubosomes (CTAB-modified PSP-Cubs) by response surface methodology (RSM). Glyceryl-monooleate (GMO) was used as the lipid base for the cubosomes. The optimal preparation conditions of CTAB-modified PSP-Cubs were as follows: the mass percentage of PSP to GMO (X 1 ), Poloxamer 407 (F127) to GMO (X 2 ) and water to GMO (X 3 ) was 1.4%, 9% and 50%, respectively. The encapsulation efficiency (EE) of CTAB-modified PSP-Cubs under the experimental conditions was 62.4 ± 3.6%, which was close to our predicted value. The particle size, polydispersity index (PDI) and zeta potential of CTAB-modified PSP-Cubs were 427.7 ± 8.0 nm , 0.236 ± 0.024 and 19.2 ± 0.4 mV, respectively. Specific modifications were able to slow down in vitro release behaviors and reduce cytotoxicity to some extent. The effects of CTAB-modified PSP-Cubs on splenic lymphocytes were also investigated. When splenic lymphocytes were treated with CTAB-modified PSP-Cubs together with LPS or PHA , the results showed more favorable effects on cellular proliferation than blank cubosomes or free PSP at certain concentrations.
研究领域:
药剂学
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免疫学
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生物技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL SCIENCES
·
2019
·Vol. 35
·期 2
·133-140
·
IF 1.618
Longjie ZHONG,Jiadi SUN,Ying GAN,Shuqi ZHOU,Zijian WAN,Quchao ZOU,Kaiqi SU,Ping WANG
Saxitoxin (STX) is one of the paralytic shellfish poisons (PSP) that endanger people’s health. It is necessary to develop methods for the on-site rapid detection for STX in order to prevent safety accidents. An enzyme-linked immunosorbent assay (ELISA) is timesaving and effective, but it is not suitable for large-scale in-field tests due to the expensiveness of commercial ELISA kits and the bulkiness of a microtiter plate reader (MTPR). In this study, a portable smartphone-based colorimetric analyzer (SBCA) with a cost-effictive enhanced gold nanoparticle-based ELISA (EGNB-ELISA) was proposed for STX detection. In a bicinchoninic acid (BCA) protein assay (R2 = 0.9939) and a glucose assay (R2 = 0.9937), SBCA was shown to be in good agreement with MTPR. EGNB-ELISA had a 12.5-fold lower detection limit (0.4 ng/mL) and a lower detection range (1 – 50 ng/mL, Y = 0.4037X + 0.3564, R2 = 0.9797) than the classical ELISA. The recovery rate ranged over 89.1 – 112.2%. The whole detection system, combining both homemade SBCA and ENGB-ELISA, is expected to satisfy the needs of on-site STX sample tests to guarantee seafood safety.
研究领域:
食品安全
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分析化学
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纳米技术
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环境科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）