BIOSENSORS & BIOELECTRONICS
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2014
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IF 6.451
Liuliu Gao,Junlin He,Wailan Xu,Jing Zhang,Junmin Hui,Yanlei Guo,Wenjuan Li,Chao Yu
α2,6-Sialylated glycans are crucial molecular targets for cancer diagnosis and clinical research. In this work, a novel ultrasensitive electrochemical biosensor was fabricated based on a graphite oxide (GO), Prussian blue (PB), and PTC-NH 2 (an ammonolysis product of 3,4,9,10-perylenetetracarboxylic dianhydride) nanocomposite for the selective detection of α2,6-sialylated glycans. To increase the sensitivity of the electrochemical biosensor, gold nanoparticles (GNPs) were immobilized on a GO–PB–PTC-NH 2 modified glassy carbon electrode (GCE). Sambucus nigra agglutinins (SNAs), which specifically bind with α2,6-sialylated glycans, were covalently immobilized on GNPs for the sensitive detection of α2,6-sialylated glycans in serum. This proposed method can be applied to human serum, and it worked well over a broad linear range (0.1 pg mL −1 –500 ng mL −1 ) with detection limits of 0.03 pg mL −1 . Moreover, recovery of the spiked samples ranged from 100.2% to 105.0%, suggesting that this excellent electrochemical biosensor can be used for the practical detection of α2,6-sialylated glycans.
研究领域:
生物医学工程
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分析化学
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癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
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IF 7.46
Jia Ding,Fei Liu,Chunling Qi,Yunlei Zhou,Huanshun Yin,Shiyun Ai
A novel photoelectrochemical (PEC) biosensor was constructed for DNA hydroxymethylation detection based on Bi 4 NbO 8 Cl/PTC-NH 2 heterojunction and Fe-MIL-88NH 2 , where Bi 4 NbO 8 Cl/PTC-NH 2 was employed as photoactive material and Fe-MIL-88NH 2 was adopted as artificial hydrogen peroxide mimic enzyme. Based on the structure of hydroxymethylated cytosine in DNA sequence, 5-hydroxymethyl-2’-deoxycytidine (5hmdC) was employed as detected target molecule for the first time using PEC technique. After Bi 4 NbO 8 Cl/PTC-NH 2 heterojunction was modified on the substrate electrode, thioglycolic acid (TGA) was captured by forming amide bond, where the sulfhydryl group (-SH) of TGA was employed as the specific recognition of 5hmdC. Under M. HhaI catalysis, the −CH 2 OH of 5hmdC reacted selectively with the –SH. Finally, Fe-MIL-88NH 2 was modified on the electrode based on the crosslinker of 4-formylphenylboronic acid (FPBA), where FPBA possessed two active groups of formyl and boronic acid group, reacting with glycosyl of 5hmdC and amino of Fe-MIL-88NH 2 . Under the catalysis effect of Fe-MIL-88NH 2 , the insoluble substance was produced on the surface of electrodes, resulting in distinct lessening of PEC response. Under optimal conditions, the photocurrent shows a linear relationship with the logarithm value of 5hmdC concentration from 0.01-100 nM with a low detection limit of 6.48 pM. More importantly, this method can be applied to investigate the influence of exogenous phytohormone on 5hmdC content in the genomic DNA of rice seedling roots and leaves.
研究领域:
材料科学
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分析化学
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生物传感器
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生物化学
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纳米技术
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电化学
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基因组学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
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2015
·Vol. 5
·期 28
·22289-22293
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IF 3.84
Qiyi Lu,Juanjuan Zhang,Yuanya Wu,Ruo Yuan,Shihong Chen
The cathodic electrochemiluminescence (ECL) behavior of the ammonolysis product of 3,4,9,10-perylenetetracarboxylic dianhydride (PTC–NH2) in aqueous solution with K2S2O8 as the co-reactant has been, for the first time reported, in this paper. The possible mechanisms have been investigated. Based on the fact that dopamine (DA) can efficiently quench the ECL intensity of PTC–NH2, DA was chosen as a template to investigate the application of PTC–NH2–S2O82− system in the sensor field. Under the optimal conditions, we achieved the detection of DA with the detection limit of 1.6 × 10−9 M. Due to the excellent ECL behavior, PTC–NH2 could be a new class of promising material for the construction of ECL sensors.
研究领域:
分析化学
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生物传感
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2015
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IF 2.729
Wailan Xu,Junlin He,Liuliu Gao,Jing Zhang,Junmin Hui,Yanlei Guo,Wenjuan Li,Chao Yu
A simple process in which a graphene oxide (GO)–Prussian Blue (PB)-3,4,9,10-perylenetetracarboxylic dianhydride derivative (PTC-NH 2 ) nanocomposite film is spread onto a glassy carbon electrode (GCE) surface is described. Glucose oxidase (GOD) was adsorbed on the modified glass carbon electrode via the specific structure of hollow Pt nanospheres. This process was sufficient to prevent GOD from leaching away, and to improve the sensitivity of our sensor. To characterise the sensors, several techniques were employed, including transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, cyclic voltammetry (CV), electric impedance spectroscopy (EIS) and chronoamperometry. The resulting amperometric glucose biosensor exhibited a fast response time (within 5 s) and a good linear calibration range from 0.01 ± 0.06 mM to 5.23 ± 0.04 mM with a lower limit of detection of 3.3 μM ( S / N = 3). Possible interferents, including ascorbic acid, l-cysteine, dopamine and ethanol, showed almost negligible electrochemical responses, indicating the high specificity of the proposed biosensor for glucose detection. The improved performance of the proposed electrode for detecting glucose in human serum was ascribed to the high surface-to-volume ratio and the excellent conductivity of the hollow Pt nanospheres. This skilful strategy of incorporating the GO/PB/PTC-NH 2 nanocomposite film with hollow Pt nanospheres enhances the performance of the electrochemical sensor, which holds promising for application in bioassay analysis.
研究领域:
生物传感技术
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材料科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
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