BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Xianzhen Song,Xiaojian Li,Dong Wei,Rui Feng,Tao Yan,Yaoguang Wang,Xiang Ren,Bin Du,Hongmin Ma,Qin Wei
In this work, 3,4,9,10-perylenetetracar-boxylic acid (PTCA) as luminophor was grafted on the surface of graphene oxide (PTCA-GO) directly. GO exhibited large specific surface area and excellent electrical conductivity which can immobilize large amounts of PTCA to improve the electrochemiluminescence (ECL) efficiency. Moreover, gold nanoparticles (Au NPs) were anchored on the surface of PTCA-GO to immobilize primary antibodies (Ab 1 ) via Au-NH 2 bond and enhance the electron transport of PTCA-GO. CuS was used as a novel co-reaction accelerator in PTCA-K 2 S 2 O 8 system to label secondary antibodies (Ab 2 ), which can react with the coreactant (K 2 S 2 O 8 ) to produce more SO 4 •- . SiO 2 nanospheres with large specific surface area were used to load a mass of CuS and Au NPs, which can directly combine with Ab 2 and accelerate the ECL emission remarkably. Therefore, a novel sandwich-type ECL immunosensor was fabricated successfully for amyloid-β protein (Aβ) detection. Under the optimal condition, a wide detection range from 50 fg/mL to 25 ng/mL and a low detection limit of 18 fg/mL (S/N = 3) were obtained. Featuring favorable specificity, stability and reproducibility, the strategy can be a powerful analytical tool in sensitive trace detection of biomolecules in clinical analysis.
研究领域:
生物分析化学
·
医学检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Wen Zhang,Yaqin Chai,Ruo Yuan,Jing Han,Shihong Chen
This work described a two-step conjugate synthesis of a porous nanostructured materials (PTCA–Cys) composed of 3,4,9,10-perylenetetracarboxylic acid (PTCA) and l -cysteine (Cys) and then the sensor prepared by the deposited gold nanocrystals (DpAu) immobilized in this nanostructure film was developed. The interesting PTCA–Cys layer not only could provide SH-functionalized interface but also easily forms a stable thin film on the electrode surface with the porous nanostructure , thus, it is very beneficial to electrode modification. Furthermore, with the DpAu film onto PTCA–Cys modified electrode , the proposed sensors exhibited the excellent catalytic activity and the high selectivity toward the oxidation of AA , DA and UA . Moreover, scanning electron microscopy, cyclic voltammetry and different pulse voltammetry were employed to characterize the sensors. Under the optimal conditions, the linear response range for simultaneous detection of AA, DA, and UA were 20–700 μM, 2–402 μM and 0.4–252.4 μM, respectively, and the detection limits were 6.40 μM, 0.67 μM and 0.12 μM (S/N = 3). More importance, the proposed method offers promise for simple, rapid and cost-effective analysis of small biomolecules .
研究领域:
生物传感器
·
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Jia Ding,Lanlan Gao,Lulu Cao,Yunlei Zhou,Hao Wang,Jingrui Duan,Huanshun Yin,Jianchao Sun,Shiyun Ai
DNA hydroxymethylation is an important epigenetic modification, which plays crucial roles in many biological processes, such as gene expression and cell differentiation. Herein, a novel photoelectrochemical immunosensor was built for detecting 5-hydroxymethylcytosine (5hmC), where the perovskite Bi 4 TaO 8 Cl sensitized by 3,4,9,10-perylenetetracarboxylicacid (PTCA) was implemented as photoactive material, anti-5hmC antibody was served as target recognition reagent, ZrO 2 was used for connecting more modifications, and ferrocene was adopted as signal amplification unit. The prepared nanomaterials of Bi 4 TaO 8 Cl, PTCA and ZrO 2 were well characterized. The possible mechanism for the improved PEC response of Bi 4 TaO 8 Cl sensitized by PTCA was investigated carefully, indicated that the matched energy band facilitated the migration of photogenerated electron. In addition, the possible mechanism for signal amplification caused by ferrocene was investigated, showing that ferrocene acted as electron donor to accelerate electron transfer. Under the optimal condition, the biosensor manifested wide detection range from 0.02 to 100 nM with the low detection limit of 5.2 pM (S/N = 3). Moreover, the applicability of the biosensor was demonstrated by investigating the effect of heavy metal Cd 2+ and perfluorooctanoic acid on 5hmC content in the leaves and roots of wheat seedlings, as well as the effect of ALV-J on 5hmC content in chicken serum.
研究领域:
生物化学
·
分析化学
·
材料科学
·
环境科学
·
分子生物学
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 7
·1-10
·
IF 5.833
Li Jingxian,Shan Xueling,Jiang Ding,Wang Wenchang,Xu Fangmin,Chen Zhidong
The combination of localized surface plasmon resonance (LSPR) and electrochemiluminescence (ECL) can be an effective way to amplify the signal intensity. In this work, an ECL aptasensor with 3,4,9,10-perylenetetracarboxylic acid–decorated cobalt phosphate (denoted as PTCA/CoP) as the ECL emitter and Au nanoparticles (NPs) as plasma was proposed for diclofenac assay. The prepared PTCA/CoP with special 1D/2D structure exhibited good ability and excellent ECL performance. The diclofenac aptamer acted as a bridge to link the PTCA/CoP and Au NPs; thus, the ECL performance of PTCA/CoP was greatly improved due to the plasma effect of Au NPs. Besides, it was found that the ECL signal of the aptasensor was obviously quenched by the introduction of diclofenac, which might be due to the transformation from the LSPR process to the resonance energy transform (RET) process. Under optimal conditions, the difference of ECL intensity was negatively correlated with the concentration of diclofenac in the range 0.1 pM to 10 μM with a low detection limit of 0.072 pM at the potential of −1.8 V vs. Ag/AgCl ( S / N = 3). The aptasensor was proved to be suitable for the detection of diclofenac in real samples, suggesting its great practicability. Graphical abstract
研究领域:
分析化学
·
纳米技术
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Zhi Luo,Yusheng Wu,Dongmiao Qin,Shuo Meng,Chujie Zeng,Biyang Deng
A luminescent reagent, 3,4,9,10-perylenetetracarboxylic acid (PTCA), was linked to a metal–organic framework (MOF) Fe(III)-MIL-88B-NH 2 through an amide reaction to form a [email protected] (III)-MIL-88B-NH 2 complex, and then gold nanoparticles (AuNPs) were grown in situ. Thus, the [email protected] (III)-MIL-88B-NH 2 @Au electrochemiluminescence (ECL) probe was obtained. The MOF of 2-aminoterephthalic acid with large specific surface area, pore sizes, and structures, which served both as the carrier and a co-reaction promoter, promoted the conversion of more S 2 O 8 2- into SO 4 •- , further enhancing the ECL signal of PTCA. An electrochemiluminescence resonance energy transfer (ECL-RET) platform was designed for the ultrasensitive and highly selective detection of cystatin C (CysC). The developed ECL-RET biosensor presented a linear range from 0.01 pg/mL to 100 ng/mL and a detection limit of 2.2 fg/mL (S/N = 3). Moreover, all the results showed that the developed ECL-RET biosensor broadened the application in the fields of biosensing and medical diagnosis.
研究领域:
分析化学
·
纳米技术
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Han Zhang,Fumei Zuo,Xingrong Tan,Shenghao Xu,Ruo Yuan,Shihong Chen
An electrochemiluminescencent (ECL) biosensor was designed for the determination of insulin using a novel ECL resonance energy transfer (ECL-RET) strategy. In this strategy, carboxyl poly(9,9-dioctyfluorenyl-2,7-diyl) dots (PFO dots) were worked as ECL donor and 3,4,9,10-perylenetetracar-boxylic acid (PTCA) exploited as ECL acceptor, and hydrogen peroxide (H 2 O 2 ) employed as the coreactant. The ECL donor and ECL acceptor were separately labeled with primary antibody (Ab 1 ) and secondary antibody (Ab 2 ), forming a sensing interface to the analyte target , insulin. In this expected sandwich-type ECL biosensor, PFO dots acted as sensing platform and PTCA employed as labels to quench the ECL emission of PFO dots. During the determination process, ECL signal of PFO dots was decreased in a gradual way by the increase of insulin concentration, and the quenching mechanism was also investigated. Under the optimal experimental conditions, the constructed biosensor exhibited an excellent performance, including a wide linear range from 1.0 × 10 −5 ng/mL to 1.0 × 10 2 ng/mL, low detection limit of 3.0 × 10 −6 ng/mL, good stability and selectivity for the detection of insulin. This pair of PFO-PTCA, as a new donor-acceptor pair in ECL-RET system, would provide a promising platform for bioanalysis in ECL field.
研究领域:
生物传感
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·Vol. 98
·期 33
·24420-24429
·
IF 7.3
Fan Jinghan,Sun Jiameng,Yu Xi,Nie Zongxiu
Micro/nanoplastics are widely detected in environmental
and biological
systems. However, methods for their direct mass spectrometric detection in vivo remain underdeveloped, limiting the simultaneous
analysis of their tissue distribution and associated metabolic responses.
In this study, we developed a perylene-3,4,9,10-tetracarboxylic acid
(PTCA)-labeling strategy for polystyrene (PS) nanospheres and established
a direct laser desorption/ionization mass spectrometry imaging (LDI-MSI)
approach for in vivo tracking. On the same tissue
section, PS–PTCA was first imaged by LDI-MSI, followed by matrix
application to obtain complementary metabolomic information. In an
intravenous model, PS–PTCA showed lung-dominant distribution
and time-dependent metabolic responses, with acute purine perturbation
followed by shifts in amino acid and nitrogen metabolism. Under an
inhalation-relevant exposure setting established by intratracheal
instillation, PS–PTCA was confined to the lungs and persisted
for weeks, accompanied by sustained metabolic alterations. This work
provides a practical MSI framework for directly mapping in vivo PS biodistribution, together with associated metabolic
responses.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2013
·
IF 4.387
Hongbo Li,Jing Li,Qin Xu,Zhanjun Yang,Xiaoya Hu
A novel visible light sensitized photoelectrochemical sensing platform was constructed based on the perylene-3,4,9,10-tetracarboxylic acid/titanium dioxide (PTCA/TiO 2 ) heterojunction as the photoelectric beacon. PTCA was synthesized via facile steps of hydrolysis and neutralization reaction, and then the PTCA/TiO 2 heterojunction was easily prepared by coating PTCA on nano-TiO 2 surface. The resulting photoelectric beacon was characterized by transmission electron microscope, scanning electron microscopy, X-ray diffractometry, FTIR spectroscopy, and ultraviolet and visible spectrophotometer. Using parathion-methyl as a model, after a simple hydrolyzation process, p-nitrophenol as the hydrolysate of parathion-methyl could be obtained, the fabricated derivative photoelectrochemical sensor showed good performances with a rapid response, instrument simple and portable, low detection limit (0.08 nmol L −1 ) at a signal-to-noise ratio of 3, and good selectivity against other pesticides and possible interferences. It had been successfully applied to the detection of parathion-methyl in green vegetables and the results agreed well with that by GC–MS. This strategy not only extends the application of PTCA, but also presents a simple, economic and novel methodology for photoelectrochemical sensing.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 2
·1-11
·
IF 5.833
Li Jingxian,Jiang Ding,Shan Xueling,Wang Wenchang,Ou Guofu,Jin Haozhe,Chen Zhidong
A novel aptasensor has been fabricated based on the resonance energy transform (RET) system from MoS 2 QDs-PATP/PTCA (donor) to NH 2 -UiO-66 (acceptor). The electrochemiluminescence (ECL) signal of PTCA was greatly amplified due to the decoration of MoS 2 QDs-PATP, and the NH 2 -UiO-66 was utilized to label the signal probe DNA (pDNA), which hybridizes with the exposed aptamer anchored on the surface of MoS 2 QDs-PATP/PTCA. With the target acetamiprid, the specific binding of acetamiprid to aptamer causes the connection between the donor and the acceptor to be interrupted and produce an “on” ECL signal. Thus, an “off-on” ECL sensing platform for sensitive and selective acetamiprid assay was designed. Under the optimal condition, the ECL signal of the aptasensor was found to be linearly related to the logarithm of the acetamiprid concentration ranging from 0.1 fM to 0.1 μM with a detection limit of 0.064 fM. More importantly, the recovery rate of the ECL aptasensor was calculated to be 98.7 ~ 106% with a RSD lower 5.1% for the residual acetamiprid assay in real food samples, which indicated that the aptasensor has high potential for practical applications. Graphical abstract
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Wei-Jia Zeng,Ni Liao,Yan-Mei Lei,Jing Zhao,Ya-Qin Chai,Ruo Yuan,Ying Zhuo
In this work, hemin was firstly used as electrochemically regenerable co-reaction accelerator for signal amplification to develop an ultrasensitive aptasensor for Aflatoxin M1 (AFM1) detection. Initially, the perylenetetracarboxylic acid (PTCA) was directly employed as luminophore to construct the ECL sensing nano-platform by combining Au nanoparticles (Au NPs) for immobilizing thiol-terminated hairpin probe (H1). Then with the help of hairpin H2, H3, the AFM1-catalyzed hairpin assembly (CHA) was executed to produce the H1-H3 duplex, which could further initiate the hybridization chain reaction (HCR) to generate dendritic DNA polymers consisting of G-rich sequence for capturing large quantities of hemin on the electrode surface . Herein, hemin as electrochemically regenerable co-reaction accelerator could interact with the co-reactant (S 2 O 8 2− ) to obviously improve the luminous efficiency of the PTCA. Therefore, a strong and stable ECL signal was achieved by the employment of hemin as electrochemically regenerable co-reaction accelerator. The proposed aptasensor determined AFM1 down to 0.09 pg mL −1 within a linear range of 0.4 pg mL −1 to 400 ng mL −1 . With excellent sensitivity and stability, the strategy provided an efficient and simple method for the trace detection of biomolecules in clinical analysis.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2015
·Vol. 62
·期 8
·739-746
·
IF 0.648
Feng Li,Yaqin Chai,Ruo Yuan,Xin Li,Yuqiao Yang
In this study, a nanocomposite of 3, 4, 9, 10-perylenetetracarboxylic acid and L-cysteine (PTCA-Cys) with satisfactory water-solubility and film-forming ability was prepared and worked as substrate for modifying the glassy carbon electrode. Then, gold nanoparticles (AuNPs) were immobilized to achieve a PTCA-Cys-AuNPs modified electrode which provided more reaction positions on the sensor. Scanning electron microscopy, transmission electron microscopy, cyclic voltammetry and different pulse voltammetry were employed to characterize the assembly process of the sensor. The constructed sensor displayed desirable sensitivity, selectivity and stability towards the simultaneous detection of ascorbic acid (AA), dopamine (DA) and uric acid (UA). Under the optimal experimental conditions, the oxidation peaks of AA, DA and UA appeared at 64, 240 and 376 mV, respectively. The corresponding linear response ranges were 3.2–435, 0.04–100 and 0.80–297 μM, and the detection limits were 1.1, 0.010 and 0.27 μM (S/N=3), respectively.
研究领域:
电分析化学
·
纳米材料
·
生物传感器
·
环境与生物医学分析
✓ 已验证
DOI
高影响因子
ANALYST
·
2020
·Vol. 145
·期 23
·7616-7622
·
IF 3.978
Jingxian Li,Xueling Shan,Ding Jiang,Yuru Wang,Wenchang Wang,Zhidong Chen
In this work, a solid-state electrochemiluminescence (ECL) sensor based on resonance energy transfer (RET) was proposed using MoS2QDs@g-C3N4 as a donor and NH2-SiO2@PTCA as an acceptor. Herein, MoS2QDs could significantly facilitate the stability and efficiency of the ECL of g-C3N4. PTCA provided a large platform to anchor NH2-SiO2 nanoparticles. The prepared MoS2QDs@g-C3N4 exhibited good spectral overlap with the UV–vis absorption spectrum of NH2-SiO2@PTCA. Based on this, we designed an “off–on” ECL sensing strategy for sensitive and selective detection of glutathione (GSH). Under the best conditions, the linear range of the sensor for GSH detection was from 0.001 to 100 μM with a detection limit of 0.63 nM (S/N = 3). More importantly, GSH in commercial samples can be detected using the proposed sensor, which indicated its superior detection capabilities and potential application value in commercial medicines.
研究领域:
分析化学
·
生物传感
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）