Clinical Oral Investigations
·
2024
·Vol. 28
·期 10
·1-14
·
IF 3.1
Liu Runze,Liu Pei,Luo Yi,Fan Wei,Fan Bing
Objectives The mannose phosphotransferase system (Man-PTS) plays crucial roles in the adaptive metabolic activity of Enterococcus faecalis ( E. faecalis ) in adverse environments. The aim of this study was to evaluate the role of Man-PTS in the alkaline resistance of E. faecalis against calcium hydroxide (CH) and the effect of metformin (Met) on the alkaline resistance of E. faecalis to CH. Materials and methods The regulatory role of Man-PTS EII in the alkaline resistance of E. faecalis was firstly investigated using a wild-type highly alkaline-resistant E. faecalis XS 003, standard ATCC 29212 and Man-PTS EIID gene deficient (△ mptD ) and overexpressing (+ mptD ) strains of E. faecalis . RNA sequencing of Met-treated E. faecalis was performed to further validate the effect of Met on Man-PTS. The effect of Met on CH resistance of E. faecalis was verified by evaluating the survival, membrane potential and permeability, intracellular pH and ATP, and the expression of Man-PTS EII and membrane transporter-related genes of E. faecalis . The effect of Met on the ability of CH to remove E. faecalis biofilm on the dentin surface was also tested. The in vivo therapeutic effect of Met plus CH (CHM) was further investigated in a rat apical periodontitis model induced by E. faecalis XS 003. Results Man-PTS EII significantly promoted the survival ability of E. faecalis in CH and enhanced its resistance to CH. The inhibition of Man-PTS EII by Met resulted in reduced alkaline resistance of E. faecalis in the presence of CH, while also enhancing the antimicrobial properties of CH against E. faecalis biofilm on dentin. Additionally, Met plus CH showed the synergistically promoted intra-canal E. faecalis infection control and healing of periapical lesion in rats. Conclusions Met could significantly reduce the alkaline resistance of E. faecalis against CH through the modulation of Man-PTS EII, and improved the antibacterial effect of CH against E. faecalis infection both in vitro and in vivo. Clinical relevance Met could significantly enhance the ability of CH to control E. faecalis infection through reducing the alkaline resistance of E. faecalis .
研究领域:
微生物学
·
药理学
·
牙科学
·
传染病学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yaozhi Zhang,Zhijia Wang,ShuChang Yao,Xiaoyu Lin,Xiang Zhang,Xinru Tan,Liuyang Zhang,Ran Xu,Yihang Zhao,Chen Zhao,Fuhao Chu,Wenguang Jing,Xuemei Huang,Penglong Wang
The global spread of Staphylococcus aureus ( S. aureus ) not only causes significant economic losses but also poses a serious threat to public health. Consequently, there is an urgent need for multidimensional strategies to develop antimicrobial dressings to combat bacterial infections. In response, we have developed a plant polysaccharide antibacterial hydrogel formed through the self-assembly of edible Kudzu powder (KP) and the natural star molecule berberine (BBR). Rheological tests show that natural polysaccharide KP-BBR hydrogel (BBR@KP) exhibits excellent injectability and adhesion. And the degradation of the hydrogel exceeded 90 % within 3 days. The synergistic effect of these two ingredients enhances the antibacterial activity of BBR and can increase the MIC of BBR to 0.05 mM. Specifically, KP promotes the affinity of the Phosphoenolpyruvate Phosphotransferase System (PTS) of S. aureus , enabling KP, with its bioadhesive properties, to adhere to the bacterial surface and continuously release BBR. Subsequently, BBR effectively exerts its antibacterial effect by inhibiting the synthesis of histidine and isoleucine. Furthermore, the BBR@KP hydrogel exhibits negligible cytotoxicity and hemolytic toxicity, underscoring its favorable biosafety profile. This synergistic natural antibacterial hydrogel, formulated through a green and straightforward methodology, not only holds promise for broad clinical applications but also provides novel perspectives for the utilization and development of plant polysaccharides in the biomedical field.
研究领域:
生物医学工程
·
药学
·
微生物学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Xiaolan Yang,Jitao Li,Qiulan Li,Dezhi Yang,Yaling Yang
The amorphous Cu-containing phosphomolybdate (Cu-PTs) composite with high peroxidase (POD)-like activity at neutral conditions was explored as biosensors for raloxifene (RAF) detection. The strong attraction between negatively charged Cu-PTs and positively charged substrates 3,3′,5,5′-tetramethylbenzidine (TMB), as well as the acceleration of the conversion of active Cu + /Cu 2+ by the Cu/W bimetallic redox couples were demonstrated to play significant roles in POD-like activity in physiological environment. When RAF is presence, it can bind to the surface of Cu-PTs and changes the chemical signal on the material surface, leading to the decreased POD-like activity. Based on this, a colorimetric method was established for the sensitive assay of RAF with a lower limit of detection (LOD) of 0.025 mg/L and good recovery from 90.13% to 108.9%. This work paves a new way to design a POD-like colorimetric protocol for tracing RAF in pharmaceutical products and environmental samples.
研究领域:
分析化学
·
生物传感
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Qiyilan Guang,Baoyu Huang,Jun Yu,Maryam Bonyani,Mohammad Moaddeli,Mansour Kanani,Ali Mirzaei,Hyoun Woo Kim,Sang Sub Kim,Xiaogan Li
In this paper, platinum sulfide decorated WS 2 microflakes (PtS/WS 2 ) heterostructure nanocomposites were prepared by an in-situ chemical reduction process. The chemiresistive-type gas sensor based on the synthesized PtS/WS 2 heterostructure nanocomposites exhibited a significantly improved sensing properties compared to that of the pure WS 2 microflakes at different concentration of NH 3 from 1 ppm to 10 ppm at room temperature . As the concentrations of PtS nanoparticles decorated on the surface of WS 2 microflakes increased, the response of the PtS/WS 2 heterostructure sensor to ammonia firstly increased when the concentration of PtS increased up to 1 wt%, whereas above 1%, the response decreased instead. Moreover, it was found that the response speeds of the PtS-decorated WS 2 were significantly improved compared to that pure WS 2 . The sensors also exhibited excellent selectivity to formaldehyde, toluene, acetone , methanol and ethanol at room temperature. The sensing mechanisms were studied by using the in-situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS). The enhanced sensing properties could be explained by “electronic sensitization” effect induced by the PtS/WS 2 heterostructure, being confirmed by density functional theory (DFT) calculations.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL RESEARCH
·
2022
·
IF 8.431
Jingqiu Sun,Xian Zhang,You Wu,Chengzhi Hu
Selective separation of nitrate over chloride is crucial for eutrophication mitigation and nitrogen resource recovery but remains a challenge due to their similar ionic radius and the same valence. Herein, a polypyrrole membrane electrode (PME) was fabricated by polymerization of pyrrole (Py) and p-toluenesulfonate (pTS), which was used as a working electrode in redox transistor electrodialysis . The anions in the source solution were first incorporated into the PME at reduction potentials and then released to receiving solution at oxidation potentials. Pulse widths and potentials were optimized to maximize the ion separation performance of PME, resulting in the improvement of NO 3 − /Cl − separation factor up to 6.93. The ion distributions in various depths of PME indicated that both NO 3 − and Cl − were incorporated into PME at negative potentials. Then, NO 3 − was preferentially released from PME at positive potentials, but most Cl − was retained. This was ascribed to the high binding energy between Cl − and PPy/pTS structure, which was 51.4% higher than that between NO 3 − and PPy/pTS structure. Therefore, the higher transport rate of NO 3 − in comparison with Cl − was achieved, leading to a high NO 3 − selectivity over Cl − . This work provides a promising avenue for the selective separation of nitrate over chloride, which may contribute to nitrogen resource recycling and reuse.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL PAPERS
·
2025
·
IF 2.5
Zhao Jinwen,Wang Le,Liu Xiaolan,Zhu Yanqin,Yin Qinhong
Nanozymes have been valuable tools in colorimetric analysis due to their ease of production, long-term stability, and adaptable enzymatic activity, enabling them to induce color changes in substrates. This study presents a straightforward enzyme-based colorimetric sensor for morphine ( MOR ) detection. The sensor utilized the laccase-like activity of a self-synthesized Cu, Bi-phosphotungstic acid ( Cu, Bi-PTs ) nanozyme, which was loaded with phosphotungstic acid, copper, and bismuth through a one-pot microwave method. This nanozyme was capable of catalyzing the coupling reaction of 2,4-dichlorophenol ( 2,4-DP ) and 4-aminoantipyrine ( 4-AP ), leading to the conversion of colorless phenolic compounds into red-colored products. The method demonstrated a detection limit of 1.88 μg/mL for MOR and a linear dynamic range from 3.333 to 400 μg/mL. The calibration curve, given by the equation y = 0.0015x + 0.4081, exhibited excellent linearity (R 2 = 0.9914), confirming the method's reliability for quantitative analysis. The developed colorimetric method was successfully applied to real samples, showing relative standard deviations (RSD) between 1.4 and 4.3% for MOR detection. These results demonstrated that the method was sensitive, cost-effective, and rapid, making it suitable for the efficient determination of MOR .
研究领域:
纳米技术
·
分析化学
·
生物传感器
·
环境与药物分析
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2021
·
IF 4.24
Bo Zhang,Li Chen,Jie-Yi Jin,Na Zhong,Xue Cai,Shu-Ping Zou,Hai-Yan Zhou,Zhi-Qiang Liu,Yu-Guo Zheng
: As the key precursor of coenzyme A synthesis in vivo , D-pantothenic acid plays an important role in maintaining biological function, which is widely applied in the food , pharmaceutical, and cosmetic industries. At present, it was mainly produced by chemical methods, with serious environmental pollution, therefore, it is urgent to develop an economical, practical, and environmentally friendly biological fermentation method based on microbial chassis cells. Herein, based on our previously developed D-pantothenic acid producing strains, we obtained higher yielding D-pantothenic acid producing strains by systematic metabolic engineering . First, according to the results of CRISPRi experiments, lpd were overexpressed, disrupted the non-PTS (non-phosphoenolpyruvate-pyruvate transport system) as well as enhanced the PTS (phosphoenolpyruvate-pyruvate transport system) allowing precursor accumulation. Subsequently, based on the analysis of metabolic intermediates , the pyruvate bypass pathway was blocked as well as the (R)-pantothenic acid pathway was further enhanced, increasing the titer of D-pantothenic acid to 4.60 g/L. Finally, the organic acid and branched-chain amino acid synthesis pathways were blocked, and in order to accelerate the synthesis of acetolactate and the output of D-pantothenic acid, heterologous acetolactate synthase and pantothenate transfer protein were introduced, which significantly increased the yield of D-pantothenic acid, reach to 6.33 g/L. The final strain in a 5 L bioreactor produced 32.32 g/L D-pantothenic acid with a productivity of 2.33 g/L/h and the high-yielding strain provides an effective platform for biological fermentation to produce D-pantothenic acid.
研究领域:
生物技术
·
代谢工程
·
微生物学
·
发酵技术
✓ 已验证
产品关联
DOI
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
·
2025
·
IF 3.7
Wenna Wang,Peng Zhao,Li Cai,Ting Zhao,Yingying Guo,Kexi Sun,Zuju Shu,Lijun Zhang,Zhongbo Li
Developing simple and reliable sensors for food freshness is a key goal in intelligent packaging. In this study, a ratiometric fluorescent nanofibre film was successfully developed for real-time visual monitoring of shrimp freshness. The film was constructed by embedding an amine-responsive fluorophore, fluorescein isothiocyanate (FITC) and a stable reference fluorophore, Eu (DBM) 3 Bpy, into an electrospun polyvinyl alcohol (PVA) matrix. The sensing principle relies on the pH-driven enhancement of FITC's green emission (530 nm) in the presence of biogenic amines, whereas the Eu-complex provides a constant red reference signal (610 nm). This dual-emission system creates a clear fluorescence colour change from orange to yellow-green as shrimp spoils. The sensor exhibited high selectivity and a rapid response, detecting spoilage within 6 h at 25°C. The maximum colour difference (ΔE) reached 64.78, allowing for unambiguous visual detection. This robust and easy-to-fabricate nanofibre film holds great promise for practical applications in food safety and intelligent packaging technology.
研究领域:
食品科学与技术
·
传感器与检测技术
·
智能包装
·
纳米材料
·
荧光传感
✓ 已验证
产品关联
DOI
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
·
2025
·
IF 3.7
Ran Feng,Hao-Nan Wei,Yan Liu,Han Tao,Bao-Cai Xu,Bao-Qiu Yang,Bao Zhang
There is an urgent need in the food packaging sector to develop smart packaging systems to cope with various scenarios, such as pH-indicating smart packaging systems. This study aims to explore the effect of incorporation of carbon quantum dots (CDs) into a corn starch/polyvinyl alcohol matrix to develop pH-mediated fluorescent sensing smart films. CDs with an average particle size of 5.496 nm were successfully prepared using a hydrothermal method incorporating citric acid and urea as raw materials, which are rich in hydrophilic groups, especially nitrogen-containing groups, and exhibit pH fluorescence response characteristics. The CDs presented hydrogen bonding–dominated interactions with the film while having no significant effect on the microstructure and crystal structure. They were uniformly dispersed in the films, and the loading of CDs endowed the films with UV-shielding ability, excellent mechanical properties and thermal stability. The pH-mediated fluorescence sensing function of the film was verified by simulating pH changes in a high-protein food storage environment using acetic acid and ammonia, showing a 62.91% increase in fluorescence intensity from pH 3.5 to 8.5. This work provided an idea for developing a new smart packaging system.
研究领域:
食品科学与技术
·
材料科学
·
智能包装
·
纳米材料
·
传感器与生物传感器
✓ 已验证
产品关联
DOI
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
·
2025
·
IF 3.7
Yiyong Zeng,Haixia Sui,Xinyu Liu,Haiyue Hou,Yujie Li,Muhammad Qamer Abbas,Yan Song,Daoyuan Yang,Zhenxia Du
The full chain of prepared dishes involves hot filling into the package and high-temperature steaming with packaging, which may lead to more migrants from the package into the prepared dishes due to the high temperature. This study focused on the safety of the prepared dishes packaging. First, migration tests were designed to fully simulate the entire contact process between polyethylene terephthalate/aluminium foil/polyamide/retort cast polypropylene (PET/Al/PA/RCPP) composite films packaging pouches and prepared dishes throughout the full chain, based on GB 31604.1-2023. A comparison was made with room temperature simulation tests to investigate the effects of high temperature on migrants. Gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) were used for the non-target screening of the migrants. A total of 17 non-volatile compounds were identified in the full-chain simulation, while only seven were detected in the room temperature simulation. Especially, the oligomers of inner polyamide 6 (PA 6) migrated through the RCPP layer into the simulants. Principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were employed to visualize differences in migrants between the full-chain and room temperature simulation. Based on the quantitative analysis, risk assessment was conducted. Ethylene glycol adipate cyclic oligomers exceeded the toxicological concern threshold under full-chain simulation, necessitating more refined risk assessment approaches. These results confirm that high temperature promotes the migration of substances from the packaging, suggesting that prepared dishes should avoid hot filling in the future. If conditions allow, consumers had better not heat them in their packaging to minimize exposure to migrants.
研究领域:
食品包装安全
·
食品接触材料
·
分析化学
·
迁移研究
·
风险评估
✓ 已验证
产品关联
DOI
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
·
2026
·
IF 4.7
Wenting Gao,Bin Mu,Fangfang Yang,Yalong Li,Yu Tang,Zhihe Luo,Aiqin Wang
The potential of chitosan (CS) films for the preservation has been explored, and it is recognized as one of the promising approaches to enhancing the comprehensive capability of CS films for food control after incorporation of functional nanofillers. Herein, Rhodiola rosea extract/ZnO/palygorskite (RRE/ZnO/Pal) nanocomposites were facilely prepared and incorporated into CS matrix to obtain RRE/ZnO/Pal/CS (RZPC) composite films, in which ZnO/Pal nanocomposites were synthesized by a green phyto-mediated method followed by loading of R. rosea extract via a semi-dry grinding process to form organic/inorganic bioactive nanocomposites. The composite films exhibited significantly enhanced antioxidant, antibacterial, mechanical and UV-shielding properties compared with the pure CS film, as well as the maximum scavenging activities on hydroxyl radicals and 2,2-diphenyl-1-picrylhydrazine radicals of composite films were 92.85% ± 1.59% and 56.89% ± 0.56%, respectively. The antibacterial ratios of composite films containing 6.25% of RRE/ZnO/Pal nanocomposites were 94.92% ± 2.67% and 95.48% ± 2.08% for E. coli and S. aureus , respectively. In addition, the RZPC films effectively delayed browning and deterioration of bananas. This study provides a green method for construction of bioactive nanocomposites and new strategy to design multifunctional CS films for food preservation based on the synergistic effect of organic and inorganic components.
✓ 已验证
DOI
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
·
2026
·
IF 4.7
Yi-Qi Liu,Yan Zeng,Shao-Fu Zeng,Zhi-Wei Wang,Lei Zhu,Li-Li Jin,Gui-Qin Shang,Gui-Hua Liu,Chang-Ying Hu
The high-value recycling of waste plastic is a key challenge in achieving a circular economy. This study introduces an innovative semi-chemical process for producing recycled polyethylene terephthalate (rPET) materials by copolymerizing post-consumer PET with monomers (ethylene glycol [EG] and terephthalic acid [TPA]) followed by esterification and polycondensation, thus opening a new avenue for the high-value utilisation of rPET that is distinct from conventional physical and chemical recycling approaches. The microstructure and thermal properties of rPET during the semi-chemical process were characterized using scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and Fourier-transform infrared spectroscopy with attenuated total reflection (ATR-FTIR). To evaluate the safety of the final product as a food contact material, the variations in the types and concentrations of volatile, semivolatile and nonvolatile organic compounds in rPET during the semi-chemical process were analysed via headspace gas chromatography–mass spectrometry (HS-GC–MS), gas chromatography-quadrupole tandem time-of-flight mass spectrometry (GC-QTOF/MS) and ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS). The results indicate that the esterification stage, characterised by the formation of numerous ‘cyclic polyester oligomers’ from oligomers, exhibited the lowest thermal stability. With the progression of subsequent processes (e.g., precondensation), molecular chains grow, resulting in a denser structure and a significant improvement in thermal stability. Ultimately, the rPET and its product obtained via solid-state polycondensation (SSP) possessed a smooth and dense surface, with its thermal stability, melting behaviour and chemical structure being highly consistent with those of virgin PET (vPET). ATR-FTIR analysis further confirmed that the process did not alter the fundamental chemical backbone of PET. Following treatment with esterification and polycondensation reactions, both the residual amounts and the number of species of volatile, semivolatile and nonvolatile substances were effectively controlled. The resulting food-contact rPET material contained only three volatile substances (ethenylbenzene, nonanal, EG), one semivolatile substance (bis(2-hydroxyethyl) terephthalate) and one nonvolatile substance (line (TPA-EG) 2 ). This recycling process significantly enhances the safety of rPET, laying a theoretical foundation for its subsequent research and development in food-contact related fields.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）