Frontiers in Plant Science
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2022
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IF 6.627
Zheng Xiaowen,Yin Minzhen,Chu Shanshan,Yang Mei,Yang Zhengyang,Zhu Yuejian,Huang Luqi,Peng Huasheng
Paeonia lactiflora Pall. is a world-famous ornamental plant, whose roots have been used as an important traditional Chinese medicine, Shaoyao, to treat diseases for more than 1,000 years. Because of the excellent curative effect of Shaoyao, its quality has attracted wide attention, however, there is a lack of comprehensive research on the different influencing factors of quality of Shaoyao. In this study, ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS) and high-performance liquid chromatography with diode-array detection (HPLC-DAD) were utilized to systematically analyze the Shaoyao of different ages, diameters and roots with “pockmarks.” 60 metabolites were detected and identified from Shaoyao using the UPLC-Q/TOF-MS, of which 20 potential quality markers of dissected roots with and without “pockmarks” were selected for the first time using the orthogonal partial least squares discriminant analysis (OPLS-DA) and the variable importance for projection (VIP) plot. Then, a selective and accurate HPLC-DAD quantitative assay has been developed for the simultaneous determination of 11 bioactive components in Shaoyao. The results showed that the total content of five monoterpene glycosides including oxypaeoniflorin, albiflorin, paeoniflorin, lactiflorin, and benzoylpaeoniflorin and six phenols including gallic acid, catechin, methyl gallate, ethyl gallate, apiopaeonoside and benzoic acid in the 3-year-old Shaoyao was higher than that of 4-year-old and 5-year-old Shaoyao. In Shaoyao of the same age, the total content of five monoterpene glycosides and six phenols decreased with an increase in diameter. In addition, regardless of whether it is a whole or a divided root, the contents of five monoterpene glycosides and six phenols in Shaoyao with “pockmarks” were higher than those of Shaoyao without “pockmarks.” In summary, this work has explored several factors that might affect the quality of Shaoyao, and provide a guide for more comprehensive quality evaluation in its further production, processing, and rational utilization.
研究领域:
生药学
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分析化学
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中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Zhenyu Zhang,Aoyu Ren,Yuchen Liu,Minzhen Yin,Yongjun Feng,Yaowu Liu,Huasheng Peng,Shanshan Chu
Paeonia lactiflora Pall. is an economically significant plant widely used in medicine and ornamentation, with a long history of cultivation. The prevalent cultivars of medicinal P. lactiflora in China display distinct morphological traits. Nevertheless, a systematic elucidation of their quality attributes remains to be achieved. This study employed ultra-performance liquid chromatography-quadrupole-orbitrap mass spectrometry (UPLC-Q-Orbitrap-MS) and ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-QQQ-MS/MS), combined with statistical analysis to investigate the metabolites of P. lactiflora roots in 17 medicinal cultivars from five major production regions in China. Significant morphological differences were observed in petal morphology, flower color, carpel features, as well as stem and leaf characteristics. In total, 167 compounds were identified or putatively characterized, including 101 phenolics, 36 terpenoids, 14 organic acids, eight amino acids, and eight other compounds (glycosides, polyols, lipids, and carbohydrates). The DD-SIMCA model established based on one-class classification successfully distinguished monopetalous red-flowered medicinal P. lactiflora from other types and differentiated herbs from Dao-di regions from those of non-Dao-di regions. Monopetalous red-flowered cultivars, representing an abundant germplasm resource, exhibited relatively high levels of terpenoids and phenolic compounds. This study has addressed the issue of compositional differences among different cultivars, screened out high-quality medicinal cultivars of P. lactiflora , and provided a theoretical basis for germplasm breeding work. Meanwhile, these findings offer a scientific rationale for the traditional view that MR cultivars are superior in traditional medicinal applications.
研究领域:
植物化学
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药用植物学
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中药学
✓ 已验证
DOI
高影响因子
Journal of Pharmaceutical Analysis
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2025
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IF 8.9
Jiaming YUAN,Zhuoping ZHENG,Zhongkang WANG,Hao TIAN,Lingling XI,Jacques CROMMEN,Tingting ZHANG,Jincai WANG,Zhengjin JIANG
Catechol-O-methyltransferase (COMT) inhibition is a critical therapeutic strategy for Parkinson’s disease (PD), yet clinical inhibitors face limitations in bioavailability and hepatotoxicity, driving demand for novel natural scaffolds. In this study, we developed an integrated analytical platform by coupling high-resolution bioassay profiling (HRBP) and affinity-based ligand fishing system to effectively characterize bioactive compounds targeting COMT in Paeonia lactiflora Pall. (the most frequently used core herb in tradition Chinese medicine prescriptions for PD treatment). Parallel High-performance liquid chromatography with diode-array detection and tandem mass spectrometry (HPLC-DAD-MS/MS) coupled with nanofractionation enabled real-time bioactivity mapping via 384-well COMT inhibition assays, while semi-preparative liquid chromatography was employed to further identify co-eluted components. HRBP and immobilized COMT ligand fishing identified 16 and 21 candidates, respectively, with 5 overlapping bioactive markers. Notably, the potent inhibitors galloylpaeoniflorin (IC 50 = 16.2 ± 3.4 μM) and 1,2,3,4,6-O-pentagalloylglucose (IC 50 = 3.1 ± 0.5 μM) exhibited comparable potency to the positive control morin (IC 50 = 10.1 ± 0.7 μM). Molecular docking results further revealed the critical interactions and binding sites between the active compounds and COMT. The validated platform demonstrates significant potential for rapid discovery of plant-derived enzyme inhibitors, bridging advanced separation, bioactivity screening, and mechanistic validation in neurodegenerative therapeutic development.
研究领域:
神经药理学
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天然药物化学
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酶抑制剂开发
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代谢组学
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分子互作研究
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中药现代化
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药物筛选
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
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2022
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IF 5.988
Sun Jia-Yi,Liu Yi-Tong,Jiang Sheng-Nan,Guo Peng-Mei,Wu Xin-Yu,Yu Jia
For thousands of years, the roots of Paeonia lactiflora Pall (PLP) has been considered by traditional Chinese medicine as a drug that can improve mental or emotional disorders, including depression, anxiety and affective disorders. Unfortunately, the research on the mechanism of action and active ingredients of this beneficial drug is not comprehensive. This study focused on the activity of essential oil from PLP (EOP), systematically studied the antidepressant effect of EOP for the first time, and discussed the potential mechanism of its antidepressant effect. In this study, we used a mouse model of corticosterone (CORT)-induced depression, and found that EOP had a significant antidepressant effect on the symptoms of CORT-induced depression in mice, and significantly down-regulated the levels of CRH, ACTH and cortisol in the brain tissues of mice. In addition, we found that EOP treatment alleviated CORT-induced hippocampal neuron injury in mice In vitro experiments. It was also found that EOP could inhibit CORT-induced apoptosis and improve the proliferation ability and cell viability of PC12 cells. Further, with the help of network analysis, it was revealed that PI3K-Akt might be one of the main signaling pathways of EOP against CORT-induced hippocampal neuron apoptosis. In this study, we also found that EOP up-regulated the phosphorylation of PI3K and Akt in CORT-induced mouse hippocampal neurons and PC12 cells, and promoted the nuclear transcription of Nrf2 in CORT-induced PC12 cells. In conclusion, with the integrated approach, we demonstrated that EOP exerted anti-apoptotic effects on hippocampal neurons through PI3K/Akt/Nrf2 signaling pathway.
研究领域:
药理学
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神经科学
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传统中药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2020
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IF 3.594
Xue Zhang,Jian Yang,Chao Wang,Yakun Sun,Zhaosheng Liu,Yanping Huang,Haji Akber Aisa
1,2,3,4,6-Penta-O-galloyl- β - d -glucose ( β -PGG) is one of active component of polyphenol tannic acid in the rhizome of radix paeoniae alba , and isolating and purifying β -PGG from crude extract of Paeonia lactiflora Pall is rather challenge work. In this paper, to reduce the embedding of imprinted sites and increase the binding capacity, β -PGG imprinted monoliths were synthesized using a strategy of combination of hydrophilic macromonomer with metal ions as pivot. With a small side chain of oligoethylene glycol, oligo (ethyleneglycol) methyl ether methacrylate (OEG) was chosen as the hydrophilic macromonomer. 4-Vinylpyridine (4-VP) and ethyleneglycol dimethacrylate was used as functional monomer and crosslinker, respectively, and Ni 2+ was adopted as metallic pivot. Polymerization parameters, including the ratio of functional monomer/template, ratios of 4-VP to Ni 2+ , and ratios of OEG to 4-VP on affinity of the resulting molecularly imprinted polymer (MIP) were systematically investigated. The structure and morphology of the obtained monolithic MIP was studied by field emission scanning electron microscopy and multipoint BET measurement. The greatest imprinting factor can be achieved to 68, which gave rise to excellent selectivity of the resulting MIP. When using the MIP as SPE absorbent, the mean recoveries for β -PGG were 74.1% with purity of 98.35%. The results demonstrated that the combination of metal ion pivot with hydrophilic macromonomer is effective approach to the balance of rigidity of imprinting cavities and polymer flexibility and increase the selectivity of the resulting MIP.
研究领域:
中药化学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）