MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Jinru Zhou,Yanbo Wang,Lei Zheng,Huan Li
Seafood allergy represents a critical public health challenge, necessitating the implementation of sensitive, precise, and quantitative detection methods for seafood allergens to guarantee accurate food labeling. Parvalbumin (PV) and tropomyosin (TM) are recognized as the primary allergens in fish and shellfish. Herein, we designed a fluorescence sensing system for the simultaneous detection of PV and TM that employs aptamer-initiated catalytic hairpin assembly (CHA). In this strategy, we utilized four hairpin dimers (H1, H2, H3, and H4) as foundational elements to create the sensing scaffolds. The CHA-based fluorescent aptasensor demonstrated an extensive linear range, ranging from 0.001 μg/mL to 100 μg/mL, with detection limits of 0.29 μg/mL for PV and 0.27 μg/mL for TM. This biosensor demonstrates exceptional selectivity and stability, in addition to good accuracy. Importantly, this biosensor has been effectively utilized to quantify PV and TM in four different commercial food products, achieving satisfactory accuracy. With these advantages, our proposed CHA-based aptasensor can offer a universal tool for sensitive and precise detection of food allergens in commercial foods throughout the supply chain.
研究领域:
生物化学
·
食品安全检测
·
分子诊断
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DOI
高影响因子
JOURNAL OF NEUROSCIENCE
·
2024
·
IF 4.4
Mengting Liu,Yixiao Gao,Fengyuan Xin,Ying Hu,Tao Wang,Fenghua Xie,Chengjun Shao,Tianyu Li,Ningyu Wang,Kexin Yuan
The inferior colliculus (IC) represents a crucial relay station in the auditory pathway, located in the midbrain's tectum and primarily projecting to the thalamus. Despite the identification of distinct cell classes based on various biomarkers in the IC, their specific contributions to the organization of auditory tectothalamic pathways have remained poorly understood. In this study, we demonstrate that IC neurons expressing parvalbumin (ICPV+) or somatostatin (ICSOM+) represent two minimally overlapping cell classes throughout the three IC subdivisions in mice of both sexes. Strikingly, regardless of their location within the IC, these neurons predominantly project to the primary and secondary auditory thalamic nuclei, respectively. Cell class-specific input tracing suggested that ICPV+ neurons primarily receive auditory inputs, whereas ICSOM+ neurons receive significantly more inputs from the periaqueductal gray (PAG) and the superior colliculus (SC), which are sensorimotor regions critically involved in innate behaviors. Furthermore, ICPV+ neurons exhibit significant heterogeneity in both intrinsic electrophysiological properties and presynaptic terminal size compared to ICSOM+ neurons. Notably, approximately one quarter of ICPV+ neurons are inhibitory neurons, whereas all ICSOM+ neurons are excitatory neurons. Collectively, our findings suggest that parvalbumin and somatostatin expression in the IC can serve as biomarkers for two functionally distinct, parallel tectothalamic pathways. This discovery suggests an alternative way to define tectothalamic pathways and highlights the potential usefulness of Cre mice in understanding the multifaceted roles of the IC at the circuit level.Significance Statement The inferior colliculus (IC) is a crucial relay station in the ascending auditory pathway. Our findings revealed that parvalbumin-expressing (PV+) and somatostatin-expressing (SOM+) IC neurons, which are present in all IC subdivisions, represent two minimally overlapping cell classes that have distinct input-output architectures and intrinsic electrophysiological properties, suggesting their distinct roles in auditory processing. Our results preliminarily uncovered how IC neurons expressing different biomarkers could contribute to the organization of the ascending auditory pathway, offered an alternative way to investigate the working mechanisms of the central auditory system, and suggested that IC neurons expressing other biomarkers should be examined as well.
研究领域:
神经科学
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听觉系统
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细胞生物学
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神经环路
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产品关联
DOI
PubMed
高影响因子
Neuroscience Bulletin
·
2021
·Vol. 37
·期 9
·1325-1338
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IF 5.203
Zhou Xinyi,Wu Bifeng,Liu Wenhao,Xiao Qian,He Wei,Zhou Ying,Wei Pengfei,Zhang Xu,Liu Yue,Wang Jie,He Jufang,Zhang Zhigang,Li Weidong,Wang Liping,Tu Jie
A strong animal survival instinct is to approach objects and situations that are of benefit and to avoid risk. In humans, a large proportion of mental disorders are accompanied by impairments in risk avoidance. One of the most important genes involved in mental disorders is disrupted-in-schizophrenia-1 ( DISC1 ), and animal models in which this gene has some level of dysfunction show emotion-related impairments. However, it is not known whether DISC1 mouse models have an impairment in avoiding potential risks. In the present study, we used DISC1-N terminal truncation ( DISC1-N TM ) mice to investigate risk avoidance and found that these mice were impaired in risk avoidance on the elevated plus maze (EPM) and showed reduced social preference in a three-chamber social interaction test. Following EPM tests, c-Fos expression levels indicated that the nucleus accumbens (NAc) was associated with risk-avoidance behavior in DISC1-N TM mice. In addition, in vivo electrophysiological recordings following tamoxifen administration showed that the firing rates of fast-spiking neurons (FS) in the NAc were significantly lower in DISC1-N TM mice than in wild-type (WT) mice. In addition, in vitro patch clamp recording revealed that the frequency of action potentials stimulated by current injection was lower in parvalbumin (PV) neurons in the NAc of DISC1-N TM mice than in WT controls. The impairment of risk avoidance in DISC1-N TM mice was rescued using optogenetic tools that activated NAc PV neurons. Finally, inhibition of the activity of NAc PV neurons in PV-Cre mice mimicked the risk-avoidance impairment found in DISC1-N TM mice during tests on the elevated zero maze. Taken together, our findings confirm an impairment in risk avoidance in DISC1-N TM mice and suggest that reduced excitability of NAc PV neurons is responsible.
研究领域:
神经科学
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分子生物学
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行为遗传学
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精神病学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiangfei Hu,Hui Wang,Qiannan Jiang,Pingwei Wen,Yueming Hu,Zongcai Tu
The mechanism of PEF treatment (5–20 kV/cm for 0.5–2 h) changing spatial conformation and enhancing allergenicity of parvalbumin of grass carp was clarified, and molecular dynamics simulations (MDS) was utilized to explore hot-spot residues and epitopes of parvalbumin-IgE complex. The phenomenon revealed that the IgG and IgE binding capacity of parvalbumin maximally enhanced by 37.24 % and 27.63 % respectively, but subsequently decreased after 20 kV/cm exposure for 1 h. Atomic force microscope showed that the particle morphology of PEF-treated parvalbumin became more regular and homogeneous, with dissociation of aggregates due to decreased hydrophobic interaction. Furthermore, 5–20 kV/cm treatments promoted conformation unfolded and de-spiralization, rendering it easy for target antibodies to interact with related epitopes. MDS showed that the critical epitopes between parvalbumin and IgE were phenylalanine58, glutamate60–61, aspartate91, aspartate93-glycine96 and glutamate102 with ΔG < −1 kcal/mol, and hot-spot residues were phenylalanine, glutamate and aspartate with ΔΔG > 1 kcal/mol.
研究领域:
食品科学
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生物化学
·
免疫学
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生物物理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2019
·
IF 4.248
Yanbo Wang,Qinqin Qi,Jinru Zhou,Huan Li,Linglin Fu
Fish has been recognized as one of the “Big-Eight” allergenic food sources with parvalbumin (PV) being one of the major allergens. Here, we report a novel immunomagnetic beads-derived enzyme-linked immunosorbent assay (ELISA) method which employs antibody functionalized graphene oxide (GO) and gold nanoparticles (AuNPs) to amplify the detection signal of PV. The GO and AuNPs are modified with monoclonal antibody (mAb) and HRP-labeled secondary antibody respectively to detect the PV captured by amino-functionalized magnetic beads. With the amplification effect of the antibody modified GO and AuNPs, the proposed ELISA could selectively detect PV with a detection limit as low as 4.29 ng/mL when using pure PV. Moreover, the recovery rates are found to range from 89.82% to 115.37% in the spiked samples. The feasibility in real-world fish sample analysis further shows that the proposed immunomagnetic beads-derived ELISA is a highly promising approach for PV detection and pave a new way for food safety analysis.
研究领域:
食品科学
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纳米技术
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分析化学
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免疫学
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2021
·
IF 6.475
Mengke Zhang,Mengyin Li,Yan Zhao,Naifeng Xu,Lanlan Peng,Yuanfeng Wang,Xinlin Wei
In this study, a rapid sandwich immunochromatographic assay (ICA) was developed to detect parvalbumin (PV). Firstly, two optimum primary monoclonal antibody (mAb) against PV had been screened out: mAb1 was used as the capture antibody, and mAb2 conjugated to Fe 3 O 4 /Au nanoparticles (Fe 3 O 4 /AuNPs) that served as a detection reagent. Using this pair of mAbs, a sandwich ICA strip based on Fe 3 O 4 /AuNPs was developed. The results showed that the color intensity of test line positively correlated with the PV concentration in the standard or spiked sample. The limit of detection for qualitative (LOD) and quantitative detection (LOQ) were 2 ng/mL and 0.691 ng/mL, respectively. Besides, the detection time of this ICA strip was within 15 min. The recovery rates ranged from 104.0% to 117.4%, within an acceptable level (80–120%). Moreover, the developed assay also showed high cross reaction in different fish species. These results demonstrated that the established test strip has the potential to be used as a rapid screening tool for large scale determination of PV in foodstuffs.
研究领域:
免疫学
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食品科学
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纳米技术
·
过敏学
✓ 已验证
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 12
·期 1
·52
·
IF 5.561
Jingjing Tai,Dan Qiao,Xue Huang,Huang Hu,Wanzheng Li,Xinle Liang,Fuming Zhang,Yanbin Lu,Hong Zhang
This study was aimed to investigate the structural and immunological properties of parvalbumin from mandarin fish during the Maillard reaction. The microwave-assisted the Maillard reaction was optimized by orthogonal designed experiments. The results showed that the type of sugar and heating time had a significant effect on the Maillard reaction (p< 0.05). The SDS-PAGE analysis displayed that the molecular weight of parvalbumin in mandarin fish changed after being glycated with the Maillard reaction. The glycated parvalbumin was analyzed by Nano-LC-MS/MS and eleven glycation sites as well as five glycation groups were identified. By using the indirect competitive ELISA method, it was found that microwave heating gave a higher desensitization ability of mandarin fish parvalbumin than induction cooker did. In vitro gastric digestion experiments showed that microwave-heated parvalbumin was proved to be digested more easily than that cooked by induction cookers. The microwave-assisted Maillard reaction modified the structure of parvalbumin and reduced the immunoreactivity of parvalbumin of mandarin fish.Keywords:parvalbumin;Maillard reaction;glycation site;structural and immunological properties;in vitro
研究领域:
食品科学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Neuroscience Bulletin
·
2023
·
IF 5.6
Song Changgeng,Zhao Yan,Zhang Jiajia,Dong Ziyi,Kang Xin,Pan Yuqi,Du Jinle,Gao Yiting,Zhang Haifeng,Xi Ye,Ding Hui,Kuang Fang,Wang Wenting,Luo Ceng,Zhang Zhengping,Zhao Qinpeng,Yang Jiazhou,Jiang Wen,Wu Shengxi,Gao Fang
Parvalbumin interneurons belong to the major types of GABAergic interneurons. Although the distribution and pathological alterations of parvalbumin interneuron somata have been widely studied, the distribution and vulnerability of the neurites and fibers extending from parvalbumin interneurons have not been detailly interrogated. Through the Cre recombinase-reporter system, we visualized parvalbumin-positive fibers and thoroughly investigated their spatial distribution in the mouse brain. We found that parvalbumin fibers are widely distributed in the brain with specific morphological characteristics in different regions, among which the cortex and thalamus exhibited the most intense parvalbumin signals. In regions such as the striatum and optic tract, even long-range thick parvalbumin projections were detected. Furthermore, in mouse models of temporal lobe epilepsy and Parkinson’s disease, parvalbumin fibers suffered both massive and subtle morphological alterations. Our study provides an overview of parvalbumin fibers in the brain and emphasizes the potential pathological implications of parvalbumin fiber alterations.
研究领域:
神经科学
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神经解剖学
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病理学
·
神经疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Innovative Food Science & Emerging Technologies
·
2025
·
IF 6.8
Xiangfei Hu,Hui Wang,Qiannan Jiang,Pingwei Wen,Jie Cheng,Haibin Liu,Yueming Hu,Zongcai Tu
The mechanism of H 2 O 2 combined pulsed electric field (PEF) treatment stimulating oxidation and decreasing allergenicity of parvalbumin of grass carp and tropomyosin of king prawn was clarified. Compared to H 2 O 2 treatment alone, the IgG-binding capacity of parvalbumin and tropomyosin significantly decreased by 21.87 % and 81.18 % after H 2 O 2 combined PEF treatment, while the IgE binding capacity reduced by 63.42 % and 76.91 %. •OH caused by electrochemical reaction triggered oxidative degradation of protein and disrupted their allergenic epitopes, which resulted in the structural unfolding, hydrophobic quenching and prevented the recognition by related antibody. Molecular dynamics showed that H 2 O 2 combined PEF treatment promoted •OH interaction with surface residues than alone H 2 O 2 treatment and PEF treatment, where the H 2 O 2 treatment and H 2 O 2 combined PEF treatment caused significant structural alterations of tropomyosin, which greatly converted α-helix to random coil. Furthermore, density functional theory calculation revealed that hydrogen abstraction at C α rendered more stable intermediates where the spin density of the unpaired electron was lower, while alcohol formed by addition of •OH to the side chains was pretty stable but their phenyl and imidazolyl states were more preferred than the saturated side-chains-containing ones.
研究领域:
食品科学
·
生物化学
·
过敏研究
·
生物物理学
·
计算化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）