JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2020
·Vol. 101
·期 7
·2779-2787
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IF 2.614
Wenbi Guan,Yingxin You,Jialing Li,Jiaying Hong,Haiying Wu,Yanna Rao
BACKGROUND Penicillic acid (PA) is produced by Aspergillus spp. and Penicillium spp., which are common postharvest and storage fungi of fruits. PA can be of concern for human health because of its toxicity and high fruit consumption by the population. However, no data on PA occurrence in various fruits have yet been reported. A quick, easy, cheap, effective, rugged and safe (QuEChERS) approach for PA determination in various fruits was developed and applied to explore PA incidence in fruits. RESULTS The modified QuEChERS procedure with extraction by ethyl acetate and purification by multi-walled carbon nanotubes (MWCNTs), primary secondary amine (PSA) and octadecyl silane (C18) was established to determine PA in various fruits by high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS). The average recoveries were 72.9–102.2% and relative standard deviations (RSDs) were 1.3–7.9%. A total of 161 fruits samples, including kiwi, apple, peach, grape and mandarin/orange, were collected in southern China. The incidence of PA in fruits was 14.9% and the levels of PA contamination were 0.200–0.596 μg kg −1 . Our results suggested that orange/mandarin, grape and kiwi were favorable matrices for Aspergillus spp. and Penicillium spp. to produce PA, rather than peach and apple. CONCLUSION To the best of our knowledge, this is the first report about PA contamination in various fruits in China. Our study emphasizes the necessity of the current established method, which could be used for continuous monitoring of PA and reducing the health risk to Chinese consumers. © 2020 Society of Chemical Industry
研究领域:
食品安全
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分析化学
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微生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2020
·
IF 6.306
Sumei Ling,Xiulan Li,Qiang Zhao,Rongzhi Wang,Tao Tan,Shihua Wang
The high content of Penicillic acid (PA) in the feed pose threat to human health and cause serious losses to economic wealth through the enrichment effect of the food chain. The reliable and rapidly detection of PA is of significant importance to ensure food safety. In this study, indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic test strips (ICTS) were established for PA determination based on anti-PA mAb secreted by 4H9 cell line. The linear range of ic-ELISA detection was 0.12–1.95 μg/mL, and the limit of detection (LOD) was 0.03 μg/mL. Then, conventional gold nanospheres (AuNS) with the average diameter of 20 nm were synthetized and AuNS-based strip was developed for rapidly detection of PA. The visual LOD (vLOD) of the AuNS-based strip was 3.9 μg/mL and the assay time of visual evaluation was less than 10 min without any instrument. To enhance the signal sensitivity of the ICTS, the larger size (about 85 nm) of gold nanoflowers (AuNFs) was prepared in our work, and was used as higher signal reporter to establish the AuNF-based strip for PA determination. Fortunately, the vLOD of AuNF-based strip was 0.97 μg/mL, which was approximately 4-fold lower than that of traditional AuNS-based strip. In summary, the rapid and sensitive immunoassays established in this study could be applied to detect and analyze the contamination of PA toxin in real food samples.
研究领域:
食品安全
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免疫学
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分析化学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2025
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IF 4
Meilin Li,Cuilin Huang,Liya Xiong,Xiao Yang,Charles A. Powell,Ronghui Wen,Muqing Zhang
Sugarcane leaf scald, caused by Xanthomonas albilineans , is a significant bacterial disease that poses a substantial threat to global sugar production. The quorum-sensing (QS) system in X. albilineans presents a promising target for anti-virulence strategies. Here, we demonstrate that penicillic acid exhibits potent antibacterial activity against X. albilineans , with a minimum inhibitory concentration (MIC) of 6 μg/mL and an effective concentration for 50 % inhibition (EC₅₀) of 3.703 μg/mL. Transcriptome analysis, molecular docking, and surface plasmon resonance (SPR) confirmed that penicillic acid competitively binds the sensory histidine kinase RpfC, disrupting DSF-mediated QS signaling. At sub-inhibitory concentrations (½ MIC), penicillic acid significantly suppressed twitching, swimming, and swarming motility by downregulating genes associated with type IV pili, flagellar assembly, and chemotaxis. At full MIC, direct binding of penicillic acid to the FlgE and CheW proteins further impaired motility. These findings elucidate the molecular mechanism of penicillic acid and support its potential as a quorum-sensing inhibitor for biocontrol of sugarcane leaf scald.
研究领域:
植物病理学
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微生物学
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生物化学
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分子生物学
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农学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
·
IF 4
Zuolong Yu,Beizhen Hu,Yao Chen,Chaoqun Huang,Chao Han,Yan Shen
A gas chromatography-tandem mass spectrometry (GC − MS/MS) method was established for accurately determining penicillic acid in cereals. The cereal samples were extracted using acidified acetonitrile, purified by solid-phase extraction, and derived using N,O-bis(trimethylsilyl) acetamide for GC − MS/MS analysis. Recovery experiments were conducted with low, medium, and high concentrations. The recovery rates were 72.1%–89.8%, the relative standard deviations were less than 8.6%, and the limit of quantification was 20 μg/kg. The established method was rapid, efficient, sensitive, and specific, meeting the requirements for the determination of penicillic acid in cereals. The method exhibits high sensitivity, accurate results, and stable recovery, making it suitable for detecting penicillic acid residues in cereals.
研究领域:
食品科学
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分析化学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2022
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IF 12.545
Yibo Yan,Long Jiang,Song Zhang,Xiantao Shen,Chuixiu Huang
Development of a specific “light-up” sensor for detection of psychoactive drug has been a great challenge in forensic analysis. To achieve this goal, an aggregation induced emission (AIE) functional monomer containing both phenylboronic group and double bond was synthesized for construction of molecularly imprinted polymers (MIPs) based fluorescent sensor. In this AIE-MIP sensor, the AIE fluorophore could vibrate freely in the absence of the target analyte (cathinone, CAT), while this vibration was restricted after the specific molecular recognition , leading to “light-up” character of the corresponding sensor. FT-IR and LC-MS characterizations proved the AIE monomer was successfully introduced onto AIE-MIPs. SEM analysis indicated the AIE-MIPs was ∼140 nm in diameter. Binding experiments indicated the AIE-MIPs owned high specificity towards CAT. Fluorescent studies confirmed that the “light-up” capability of the AIE-MIPs was highly selective. On this basis, the AIE-MIP sensor was applied in detecting CAT in forensic samples. The sensor reached a detection limit of 0.32 μmol L -1 and exhibited a linear range of 2–12 μmol L -1 . Compared to previously reported MIPs based electrochemical sensors and fluorescent sensors for measurement of CAT drug and its analogue, the present AIE-MIP sensor showed much higher sensitivity. To the best of our knowledge, this is the first time that an AIE functional monomer was synthesized for molecular imprinting , and also the first “light-up” AIE-MIP sensor to be reported. We believe that this versatile design of the specific “light-up” sensor can be used as a general protocol for construction of advanced sensor in various fields.
研究领域:
分析化学
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法医学
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材料科学
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
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2022
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IF 5.122
Juan Fu,Ziyang He,Xin Hu,Teng Guo,Ye Liang,Fengjie Deng,Meiying Liu,Yuanqing Wen,Xiaoyong Zhang,Yen Wei
Fluorescent materials with aggregation-induced emission (AIE) have standout as one of the most interesting candidates for biomedical applications. The development of new methods for fabrication of AIE-active materials will surely advance their applications in related fields. In this work, we reported a novel method for preparation of AIE-active luminescent polymer (Lys-PEG-TPE) through a three-component reaction involving carbon dioxide (CO 2 ) using lysine, Polyethylene glycol (PEG) and AIEgen as the reactants. On account of the introduction of PEG , Lys-PEG-TPE could well disperse in aqueous solution, while hydrophobic TPE will aggregate in the core of self-assembled nanoparticles , endowing the obvious enhancement of fluorescence intensity. Results from biological assays suggested that Lys-PEG-TPE exhibits almost no toxicity after incubation with HepG2 cells and its fluorescence can be clearly observed in cytoplasm after cell internalization. More importantly, the AIE feature of Lys-PEG-TPE could not only effectively solve the aggregation-induced quenching effect of traditional fluorescent molecules, the carboxyl groups of lysine could also be used for loading functional cationic ions (e.g., anticancer drugs or imaging agents) to fabricate multifunctional theranostic and imaging composites. Considering the benign reaction conditions, high atom economy, and well tunability of reactants of multicomponent reactions, this strategy should be of broad interesting for fabrication of multifunctional AIE-active materials for biomedical applications.
研究领域:
材料科学
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生物医学工程
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化学
✓ 已验证
产品关联
DOI
高影响因子
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