FITOTERAPIA
·
2019
·
IF 2.431
Fei He,Xiaobao Li,Jin-Hai Yu,Xiaoyong Zhang,Xuhua Nong,Guangying Chen,Kongkai Zhu,Yin-Yin Wang,Jie Bao,Hua Zhang
Five new funicone derivatives, pinophilones A–E ( 1a/1b and 2 – 4 ), along with 18 biosynthetically related known analogues ( 5 – 22 ), were obtained from the culture of a mangrove sediment-derived fungus Penicillium pinophilum SCAU037. Their structures were established by analysis of 1D/2D NMR and MS data, while the absolute configurations of the new compounds were determined by ECD calculation based on time-dependent density functional theory (TD-DFT). The dihydrofuran moiety in 1a and 1b was first reported for funicone derivatives. Compound 22 exhibited antibacterial activity against Mycobacterium smegmatis and Staphylococcus aureus with IC 50 of 23.5 and 2.6 μM, respectively, while 16 , 18 and 22 showed significant α-glucosidase inhibitory activity with IC 50 of 51.9, 78.4 and 33.8 μM, respectively.
研究领域:
微生物学
·
天然产物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2016
·
IF 4.052
Shuzhen Yang,Jie Zhou,Dongmei Li,Chunyu Shang,Litao Peng,Siyi Pan
To evaluate the structure-activity relationship of 5,7-dihydroxyflavonoids against P. italicum , we tested the antifungal activity of 23 selected 5,7-dihydroxyflavonoids against spore germination of P. italicum , and the effects of hydroxyl group, hydrogenation, methylation and glycosylation on the antifungal activity are explored. C-4′-OH and C-3-OH are active groups for the 5,7-dihydroxyflavonoids against P. italicum. We find that hydrogenation of the C2/C3 bond decreases the antifungal activity of 5,7-dihydroxyflavonoids. Antifungal activity of 5,7-dihydroxyflavonoids against P. italicum was affected by the conjugation site of glycosylation and the class of sugar moiety. The correlation between antifungal activity and the inhibition of respiration of 5,7-dihydroxyflavonoids was further evaluated. We find no significant relationship among the IC50 of 5,7-dihydroxyflavonoids on spore germination and on respiration. Some 5,7-dihydroxyflavonoids even enhance the respiration of P. italicum . This indicate respiration is not the only target for 5,7-dihydroxyflavonoids against P. italicum .
研究领域:
植物化学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2025
·
IF 1.9
Zehao Li,Zixuan Wang,Lulu Zhang,Feng Yingjie,Jinchu Yang,Haoliang Li,Weimin Song,Qiuling Wang,Yongfeng Yang,Zhenzhen Huang,Yongming Xu,Yufeng Fu,Meizhou Ding,Gang Fan,Jingnan Ren,Aiqun Yu,Jian Feng,Guijie Li,Linhua Huang,Yujiao Cheng
α-Terpineol, an abundant oxygenated monoterpene compound, shows a varied range of beneficial bioactivities. This study focused on the efficient extraction and purification of α-terpineol from the biotransformation of limonene using Penicillium digitatum DSM 62840 mutant (PdTP1-overexpressed OE2 strain). The α-terpineol was primarily distributed in the supernatant, with minimal association with the biomass. And the optimal extraction was achieved using ethyl acetate as the extractant, which can directly obtain the highest recovery of α-terpineol without sequential extractions. The equilibrium of mass transfer was quickly reached (≤20 s in vortex). Additionally, the purification method of α-terpineol using column chromatography was described, further improving the purity of product to 96.86% in gas chromatography. Structural identification of purified α-terpineol was confirmed using gas chromatography-mass spectrometry, Fourier-transform infrared spectroscopy, and nuclear magnetic resonance. This simplified and efficient strategy for the extraction and purification of α-terpineol not only provides a solid theoretical basis for the final step in the relevant study of microbial synthesis of α-terpineol but also is of great significance for its industrial application.
研究领域:
微生物学
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天然产物化学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
FeiFei Wang,Taizhi Shen,Changrong Cao,Shuo Shan,Juan Sun,Ruiyu Zhu
Penicillium expansum is a major pathogen critical for the blue mold decay and patulin production in various fruits and vegetables, particularly apples, creating an urgent need to identify safer and more effective compounds for its management. In this study, two pathogenic factors of P. expansum , PeBgl1 and PeSCP, were first time targeted for virtual screening with a database (a combination of TCM Database @ Taiwan and food ingredients database) containing 20,219 molecules, and octyl gallate (OG) was identified as a common candidate inhibitor with high binding energy. Therefore, we investigated the inhibitory impacts of OG against P. expansum and patulin production in vitro and in apples. It’s revealed that OG significantly inhibited the spore germination and mycelia growth by inducing oxidative stress and compromising cell membrane integrity, as well as restrained patulin biosynthesis via suppressing the expression level of patulin synthesis genes including patE, patL , and patK. Molecular docking and dynamics simulation indicated that OG could affect the stability and function of PeBgl1 and PeSCP mainly via Van der Waals forces and hydrogen bonds. qRT-PCR analysis further demonstrated that OG directly inhibited the expression of genes encoding for PeBgl1 and PeSCP. Finally, in vivo application of OG on apples demonstrated that OG can effectively trigger the defensive enzyme system of apples and control the blue mold decay caused by P. expansum . Overall, this study emphasizes the potential of OG as a promising natural compound for the management of blue mold decay and patulin production in the post-harvest fruits and vegetables.
研究领域:
植物病理学
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食品安全
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
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IF 6.056
Xianghua Wang,Maorun Fu,Xiaoqian Qu,Jingjing Liu,Jianwen Bu,Shaoran Feng,Handong Zhao,Wenxiao Jiao,Fei Sun
(E)-2-Hexenal is one of the most prevalent and abundant volatile substances in fruits. Many researchers have attempted fumigation with (E)-2-hexenal to control fungal diseases with poor or no results; the key is the inability to maintain an effective concentration for a long time. Therefore, a new starch-based antifungal coating with (E)-2-hexenal microcapsules was prepared. The obtained results showed that the microcapsule embedding rate reached 78.95% by orthogonal optimization experiments and that the (E)-2-hexenal-based coating was effective at reducing the lesion diameter of apple fruit during storage at 25 °C after Penicillium expansum infection, and in other fruits; it also achieved a good antifungal effect. Moreover, the activities of the main defense-related enzymes and the expression of key genes involved in the salicylic acid (SA) signaling pathway, such as CHI, GLU, PAL , NPR1 , and PR1 , were enhanced by (E)-2-hexenal-based coating treatment. These findings suggest that the (E)-2-hexenal-based coating has the dual effect of direct antifungal and induction of apple system resistance. E2H-based coating is a promising approach to control postharvest Penicillium expansum in fruit.
研究领域:
植物病理学
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食品科学
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Marine Drugs
·
2020
·Vol. 18
·期 3
·164
·
IF 4.073
Zhongbin Cheng,Wan Liu,Runzhu Fan,Shouye Han,Yuanli Li,Xiaoyun Cui,Jia Zhang,Yinan Wu,Xin Lv,Yun Zhang,Zhuhua Luo,Siti Aisyah Alias,Wei Xu,Qin Li
A chemical study of the ethyl acetate (EtOAc) extract from the deep-sea-derived fungusPenicilliumthomiiYPGA3 led to the isolation of a new austalide meroterpenoid (1) and seven known analogues (2−8), two new labdane-type diterpenoids (9and10) and a known derivative (11). The structures of new compounds1,9, and10were determined by comprehensive analyses via nuclear magnetic resonance (NMR) and mass spectroscopy (MS) data. The absolute configurations of1,9, and10were determined by comparisons of experimental electronic circular dichroism (ECD) with the calculated ECD spectra. Compound1represented the third example of austalides bearing a hydroxyl group at C-5 instead of the conserved methoxy in other known analogues. To our knowledge, diterpenoids belonging to the labdane-type were discovered from species ofPenicilliumfor the first time. Compound1showed cytotoxicity toward MDA-MB-468 cells with an IC50value of 38.9 μM. Compounds2and11exhibited inhibition against α-glucosidase with IC50values of 910 and 525 μM, respectively, being more active than the positive control acarbose (1.33 mM).Keywords:Penicillium thomiiYPGA3;deep-sea-derived fungus;austalide meroterpenoid;labdane-type diterpenoid;bioactivities
研究领域:
化学
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药理学
·
海洋生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Jianhao Tong,Hanxin Wu,Xiaohan Jiang,Jing Wang,Jingli Pang,Haonan Zhang,Ziming Xin,Jiyan Shi
The exploration of microbial resources to reduce Pb accumulation in rice attracted great attention. In this study, we found Penicillium oxalicum SL2, a Pb-tolerant strain with good capability of dissolving phosphorus and stabilizing Pb in soil, was able to colonize on the root surface of rice seedlings without additional carbon sources, and promoted the secretion of metabolites related to amino acid metabolism , organic acid metabolism, signal transduction and other pathways in rhizosphere exudates, in which the secretion of oxalate increased by 47.7 %. However, P. oxalicum SL2 increased Fe(II) proportion and Fe availability on the root surface, resulting in iron plaque content decrease. Moreover, by converting root surface Pb from Pb-Fe state to PbC 2 O 4 and Pb-P compounds, P. oxalicum SL2 increased Pb intercept capacity of iron plaque by 118.0 %. Furthermore, P. oxalicum SL2 regulated element distribution on the root surface, and reduced the relative content of Pb on the maturation zone of root tip , which was conducive to reducing Pb uptake by apoplastic pathway and the risk of Pb accumulation in root system . Our findings further revealed the interaction between P. oxalicum SL2 and rice root, providing a theoretical basis for the development and application of microbial agents in Pb-contaminated farmland.
研究领域:
微生物学
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环境科学
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农业科学
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2023
·
IF 3.8
Zhenhui Wang,Xueqian He,Ling Yang,Zhanlin Li,Zhenzhen Zhang
A one-pot/two-stage precursor-directed biosynthesis approach was used to introduce selected sulfanilamide derivatives into the talaroenamine pathway in a Yellow River wetland-derived fungus. During this approach, precursors p -methylaniline and sulfanilamide derivatives were added to the medium in two stages, improving the substrate acceptance of biosynthetic intermediate cyclohexanedione to form diverse talaroenamines. Ten new sulfa-containing talaroenamine derivatives, sulfarulumines A-J ( 1 – 10 ), were isolated and structurally characterized. Compound 2 displayed acetylcholinesterase (AChE) inhibitory effect with an IC 50 value of 4.9 µM. Molecular docking study suggested that 2 exerted inhibitory activity by binding to an active site of the enzyme.
研究领域:
真菌学
·
药物化学
·
生物合成
✓ 已验证
DOI
高影响因子
BIOLOGICAL CONTROL
·
2023
·
IF 4.2
Yu Ding,Fengjuan Liu,Jin Yang,Yingying Fan,Lvjian Yu,Zenghao Li,Na Jiang,Jing An,Ziwei Jiao,Cheng Wang
Penicillium expansum is the main causal organism of apple rot and causes huge economic losses during apple storage. Patulin (PAT) produced by P. expansum , occurs widely in apples and their products because of its water-solubility and thermal stability . In the present study, we isolated bacterial strains with antagonistic activity against P. expansum to effectively control the growth of P. expansum and production of PAT. We isolated a bacterium named YL-RY0310, which inhibited the growth of P. expansum and production of PAT in apples by 77.4% and 48.4%, respectively. Strain YL-RY0310 was 96% homologous to Bacillus mojavensis using morphological and 16S rRNA gene sequencing analyses. In addition, DNA amplification analysis using Bacillus -specific primers showed that the genes responsible for synthesis of lipopeptides of surfactin, iturins and fengycin are expressed. Oxford cup agar diffusion test showed that its crude lipopeptides could effectively inhibit the growth of P. expansum . Furthermore, strain YL-RY0310 could significantly degrade PAT; the degradation efficiencies of the intracellular and extracellular metabolites of the strain were 62.6% and 56.9%, respectively. Therefore, B. mojavensis YL-RY0310 could be used as a biocontrol agent to inhibit the growth of P. expansum and control PAT contamination in apples. This study provides a theoretical basis for a novel biological control strategy and degradation of mycotoxin.
研究领域:
植物病理学
·
食品科学
·
微生物学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Ying-Zhi Peng,Xiao-Lu Zhu,Xiao-Xiao He,Yi-Hao Chen,Xiang Lv,Le-Yun Yang,Jing Li,Wei-Guo Zhao,Jun Wang,Shuai You
Glucose oxidases (GODs) induce the catalyzation from β- d -glucose to gluconic acid in an oxygen-consuming process, providing a potential antibiotic substitution strategy. However, the inadequate properties of existing GODs in parallel hinder the antimicrobial capacity for industrial applications. In this study, PaGOD (WT) from Penicillium amagasakiense was enzymatically improved through computer-aided design based on energy optimization. Two thermostable variants A263P and K424F were selected and combined to generate variant A263P/K424F, superior in both thermostability ( t 1/2 at 60 °C increased 2.6-fold) and catalytic efficiency (2.1-fold increase in catalytic efficiency), in comparison with the WT. The molecular dynamics simulations revealed the improved rigidity of A263P/K424F is attributed to the formation of hydrogen bonds within the flexible region and the newly-formed salt bridge Lys473: Asp477, following the increased ΔΔG. For improvement of antibacterial capacity, A263P/K424F impressively lower the half maximal inhibitory concentrations (IC 50 ) to 12 and 11 mg/L respectively for Escherichia coli and Staphylococcus aureus (86.4 % and 78.8 % lower than the WT, 65.7 % and 50 % lower than erythromycin). The results indicated that the antibacterial effects of GOD can be improved through in vitro molecular modification, which could be an effective strategy to address antibacterial requirements with antibiotic-free agents.
研究领域:
生物化学
·
分子生物学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 3.9
Jufen Yan,Pei Wang,Longxin Wang,Qi Jin,Abdallah S. Ali,Yongsheng He,Ying Wang,Yuxuan Sun,Aoqiang Li,Waly Adwy,Rasha H. Ahmed,Xinya Han
Synthetic dyes from textile, paper, leather, and plastic processing, etc. are the majority of the colored effluents and have a harmful impact on human health and environment. Dye biosorption through endophytic fungi will be a challenge. A new fungus isolated from blueberry pulp was identified morphologically and genetically as Penicillium janthinellum LM5. It was utilized for the biosorption of synthetic dyes by forming mycelium particles with efficient decolorization properties in the dye solution. The results of decolorization experiments exhibited that dye type, pH, temperature and time were the important factors and the best decolorization condition on Congo red was pH 6.0, 30 ℃ and 48 h. Moreover, LM5 can achieve 90% decolorization rate even under the high salt concentrations of 200 mg/mL. Based on spectral analysis , the decolorization of Congo red was mainly attributed to the biosorption of mycelium and its pellet structure. Additionally, the fungus adsorbed Congo red can be as an excellent electrode material with a long-cycle performance and stable capacitance property, being a good way for recycling. In summary, this research employed a novel dye treatment method with simultaneous culture and decolorization through an endophytic fungus LM5, being a promising biomaterial in the application of dye wastewater treatment .
研究领域:
环境科学
·
微生物学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2020
·
IF 6.513
Yuan Long,Wenqian Huang,Qingyan Wang,Guiyan Yang
Penicillium italicum ( P. italicum ) can cause significant economic loss of fruits and vegetables. Although garlic oil (GO) is an effective antimicrobial agent, the unstability and hydrophobicity limit its use as an environmentally friendly alternative to the conventional antibiotics against P. italicum . In this study, we focused on the fabrication and characterization of a functional GO nanoemulsion (NE) using ultrasonic technique and revealed the antifungal mechanism of the GO NE on P. italicum based on morphological, structural and molecular analyses. The optimal hydrophilic lipophilic balance (HLB) value determined for GO was 14 through the combination of Tween 80 and Span 80. Then the Box-Benhnken Design (BBD) was applied to produce the GO NE and the effects of different fabrication parameters on the particle size were evaluated. The optimal GO NE was selected with the GO concentration of 5.5%, the Smix concentration of 10%, the ultrasonic time of 5 min and the power of 50%. This GO NE had the smallest particle size of 52.27 nm, the best antifungal effect and the most stability. Furthermore, the antifungal mechanism of the GO NE on P. italicum was evaluated by extracellular conductivity, micro-Raman spectra, fluorescence imaging and scanning electron microscopy (SEM) imaging. The results presented that the GO NE retained the antifungal active ingredients. The fungal cell structure and morphology were malformed after treated with the GO NE and the lipids, nucleic acids and protein of P. italicum were destructed. Finally, the optimal GO NE was applied in vivo and P. italicum in citrus was successfully inhibited. It indicated that the optimal GO NE had the better antifungal activity against P. italicum than the pure GO. Besides, the minimum inhibitory concentration (MIC) of GO after preparing into the NE was changed from 3.7% to 0.01265% with about 300 times improvement of bioavailability. Therefore, the synthetic GO NE which promoted the bioavailability of GO was recommended as a promising alternative to inhibit P. italicum in vegetables and fruits.
研究领域:
食品科学
·
微生物学
·
植物病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）