Frontiers in Microbiology
·
2022
·
IF 6.064
Li Xiaomei,Chen Fei,Xiong Yi,Guo Linna,Xu Jingjing,Lin Yanli,Ni Kuikui,Yang Fuyu
The aim of this study was to investigate the influence of Perilla frutescens, alone or in combination with Lactobacillus plantarum a214 or citric acid, on forage oat silage quality, bacterial and fungal microbiological profile during ensiling and aerobic exposure. With the exception of Perilla frutescens, all additives could improve silage quality of forage oat based on lower ammonia-nitrogen content and higher residual of water soluble carbohydrates during anaerobic fermentation compared to control silage, especially in Perilla frutescens combined with citric acid (CAPF). Lactobacillus was the dominant bacteria in all silages, while CAPF group increased the relative abundance of Lactobacillus lindneri and Lactobacillus brevis compared to control silage. The application of Perilla frutescens suppressed the relative abundance of yeasts such as Pichia fermentans and Wickerhamomyces anomalus in response to aerobic exposure, especially in CAPF treatment, leading to high acetic acids and lower dry matter loss, as well as good aerobic stability. Therefore, Perilla frutescens, alone or in combination with citric acid, has potential to improve aerobic stability of forage oat silage by shifting bacterial and fungal community composition, and can be used as new additive to prepare high-quality silage for animal production.
研究领域:
微生物学
·
动物营养学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2021
·Vol. 13
·期 2
·880-890
·
IF 5.396
Yana Zhao,Huizhen Li,Zhijun Zhang,Zhiqing Ren,Fuhan Yang
Polyphenols exhibit potential functional activities, especially rosmarinic acid (RosA) and caffeic acid (CafA). In this study, two different methods, ultrasonic-assisted ethanol extraction (60%) and ultrasound-assisted cellulase (≥15 000 Ug−1, 2%) hydrolysis, were used for the extraction of the total phenolics from 44 species of Perilla frutescens. The Folin–Ciocalteu method of detection showed that the content of the total phenolics extracted by cellulase hydrolysis was the highest and attained up to 28.00 mgGAE per gextracts for ZB1. Continuously, the extracts were purified using XDA-8 macroporous resin and medium-pressure liquid chromatography (MPLC), and the content of the total phenolics improved to 66.62 mgGAE per gextract. A high-performance liquid chromatography (HPLC) assay showed that the total polyphenols were mainly composed of gallic acid, caffeic acid, rosmarinic acid, luteolin and apigenin. Besides, a sequential XDA-8 macroporous resin combined with high-speed counter-current chromatography (HSCCC)/MPLC system was established for the simultaneous isolation and preparation of RosA (purity 98.29%) and CafA (purity 97.01%) from the extracts. Furthermore, the antibacterial activities of the total polyphenols were evaluated by the disc diffusion method and scanning electron microscopy (SEM) observation. The results verified that the total polyphenols had effective antibacterial activity on three kinds of bacteria including E. coli, S. aureus, and B. subtilis in a concentration-dependent manner. All of these results demonstrated that the ultrasound-assisted cellulase hydrolysis extraction of the total polyphenols and the proposed three-step separation of RosA and CafA gave high yields and good purity, and they exhibited effective antibacterial ability.
研究领域:
植物化学
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天然产物研究
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药理学
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产品关联
DOI
PubMed
高影响因子
Pharmaceuticals
·
2023
·Vol. 16
·期 12
·1655
·
IF 4.6
Isoo Youn,Sujin Han,Hee Jin Jung,Sang Gyun Noh,Hae Young Chung,Yean Kyoung Koo,Sunhye Shin,Eun Kyoung Seo
Perilla frutensevar.acuta(Lamiaceae) has been used to treat indigestion, asthma, and allergies in traditional medicine. In this study, luteolin 7-O-diglucuronide (1), apigenin 7-O-diglucuronide (2), and rosmarinic acid (3) were isolated from the leaves ofP. frutescensvar.acutathrough various chromatographic purification techniques. Several approaches were used to investigate the anti-inflammatory activity of the constituents (1–3) and their working mechanisms. In silico docking simulation demonstrated that1–3would work as a PPAR-α/δ/γ agonist, and in vitro PPAR-α/δ/γ transcriptional assay showed that thePerillawater extract (PWE) and3increased PPAR-α luciferase activity (1.71 and 1.61 times of the control (PPAR-α + PPRE,p< 0.001)). In the NF-κB luciferase assay,1suppressed NF-κB activity the most (56.83% at 5 µM; 74.96% at 10 µM; 79.86% at 50 µM). In addition,1and2inhibited the mRNA expression of NF-κB target genes, includingIl6,Mcp1, andTnfa, at 50 µM, and3suppressed the genes at the mRNA level in a dose-dependent manner. We report that1and2exert anti-inflammatory effects through NF-κB inhibition, and the PPAR-α/NF-κB signaling pathway is related to the anti-inflammatory activity of3.Keywords:Perilla frutescensvar.acuta;anti-inflammation;peroxisome proliferator-activated receptor;nuclear factor kappa B;flavonoid diglucuronide
研究领域:
药理学
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植物化学
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分子生物学
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DOI
PubMed
高影响因子
Rhizosphere
·
2025
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IF 3.5
Gen Li,Simiao Wang,Yijin Wang,Yibang Zhang,Qing Dang,Yashuo Tian,Longfei Li,Lijuan Wang,Li Han
Perilla frutescens is a medicinal plant that bioactive compounds such as perillaldehyde and luteolin, but cadmium (Cd) contamination threatens its safe cultivation and medicinal quality. While dark septate endophytes (DSEs) are known to increase plant stress tolerance, their strain-specific roles in regulating Cd uptake and secondary metabolite biosynthesis remain poorly understood. This study aimed to investigate three DSE strains, DSE6 ( Paraphoma radicina ), DSE7 ( P. chlamydocopiosa ), and MD3-1 ( Acrocalymma aquatica ), to determine their effects on growth, physiological traits, Cd accumulation, and key metabolite production in P. frutescens under 0, 1, 5, and 10 mg/kg Cd stress. DSE6 and DSE7 significantly reduced root Cd content and improved photosynthetic pigments, antioxidant activity, and biomass under moderate Cd levels. Notably, MD3-1 did not reduce Cd uptake but increased luteolin content by 22.67 % under high Cd stress, suggesting a decoupling between Cd accumulation and metabolite production. All three strains increased perillaldehyde content, with DSE7 showing the greatest increase (262 %) at 5 mg/kg Cd. These findings demonstrate that DSEs regulate Cd tolerance and medicinal quality via distinct mechanisms, and that internal metabolic tolerance may sustain bioactive compound synthesis under heavy metal stress. This highlights their potential as microbial agents for cultivating medicinal plants in contaminated soils.
研究领域:
植物学
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植物生理学
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微生物学
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环境毒理学
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植物化学
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产品关联
DOI
高影响因子
Heliyon
·
2024
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IF 3.4
Yanbo Wang,Yana Zhao,Yurong Guo,Wanyu Han,Zhijun Zhang,Tianyu Hou,Huizhen Li,He Li,Qinqin Wang
Perilla frutescens L. essential oil (PLEO) has antibacterial and antioxidant properties, which can effectively maintain the quality of fruits and extend their shelf life. In this study, sodium alginate and chitosan were used as wall materials, and PLEO microcapsule powder was used as the core materials to prepare PLEO hydrogel beads. The best results were obtained by using 2%w/v sodium alginate and 1.5%w/v chitosan as wall materials, with a core-to-wall ratio of 2:1 and homogenized for 15 min producing PLEO hydrogel beads with encapsulation efficiency of 82.61 %. For strawberries preservation, PLEO hydrogel beads preservation group had a better effect after 5 d of storage, showing a lower decay rate (15.71 %), better maintaining the hardness of 1.75 kg/cm 2 , and a weight loss of 3.29 %. Furthermore, organic acids and total phenols were retained more in this group, the number of microorganisms was significantly reduced, and sensory qualities were improved, especially taste and color. This study provides important insights into the application of natural preservatives in the food industry and promotes sustainable practices in food preservation.
研究领域:
食品科学
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植物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
·
2020
·Vol. 31
·期 1
·25-32
·
IF 1.399
Meiqin,Zhu Jingjing,Fan Rongrong,Wang Yujie,Lv Zhaojun,Han Yongming,Peng Jun,Zheng Xinyu,Lin Ruiyu
High specific surface area activated carbon prepared by Perilla frutescens (PFHSAAC) was modified on the surface of the glassy carbon electrode (GCE) by drop-coating method to construct a PFHSAAC/GCE. The electrochemical properties of the modified electrode were investigated in detail by some electrochemical methods such as cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Experiment results show that the modified electrode has excellent redox activity and distinction capability toward the two isomers of catechol (CC) and hydroquinone (HQ). The separation of oxidation peak potential for HQ and CC of DPV is 112.8 mV. Under the optimal conditions, the oxidation peak currents of HQ and CC show good linear relationships with their concentrations in the ranges of 1.0 × 10-6-2.0 × 10-4 and 1.0 × 10-6-1.5 × 10-4 mol L-1, respectively. The limits of detection for HQ and CC are 3.57 × 10-7 and 4.23 × 10-7 mol L-1, respectively. The PFHSAAC/GCE exhibits good stability, repeatability and sensitivity. The developed method was used to determine HQ and CC in water samples with best results.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Tingting Zhang,Hualin Wang,Dongxiu Qi,Li Xia,Linlin Li,Xingjiang Li,Shaotong Jiang
A colorimetric cellulose acetate (CA) membrane incorporated with Perilla frutescens (L.) Britt. anthocyanins (PFA) and chamomile essential oil (CO) is developed via electrospinning technique for food freshness monitoring and shelf-life extending. The moieties of PFA and CO are well-dispersed in fiber matrix by hydrogen bonds and their incorporation increases the fiber size but with no obvious influence on the fiber morphology at incorporation levels. The presence of CO enhances membrane hydrophobicity . The target membrane of CA-PFA6-CO15 (PFA6%, CO15%) has a wide color change range of pH 2–12 which is high sensitive and reversible towards external pH-stimuli. The membrane has good antibacterial activity against E. coli and S. aureus besides antioxidant activity . The release of bioactive moieties is predominantly controlled by Fickian diffusion. The target membrane can simultaneously monitor pork freshness in real-time and double the shelf-life at 25 °C, indicating its potential application in active and intelligent food packaging.
研究领域:
食品科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Plants-Basel
·
2026
·Vol. 15
·期 7
·1044
·
IF 4.7
Wei Wei,Bin Wang,Zhaoyuan Li,Yang Liu,Hongliang Ji,Zhou Wu,Guangyao Ma,Yuxuan Sun,Tiantian Zhang,Yanbing Liu,Longfeng Feng,Yue Jin,Tingting Wang,Qiuling Wang,Zhihui Gao,Jianhe Wei
Perilla frutescens(L.) Britt. (P. frutescens) is an important medicinal and aromatic plant, whose leaf color and chemotype strongly influence its medicinal quality and economic value. All the previously discovered perillene (PL)-typeP. frutescensare double-sided green, and whether the PL-type trait is tightly linked with the green-leaf trait in genetics remains to be clarified. This study aimed to address this question and attempt to create purple-leaf PL-type germplasm through perillaldehyde (PA) × PL hybridization. Three parallel experiments were conducted using purple-leaf PA-typeP. frutescensas male parents and green-leaf PL-typeP. frutescensas female parents. Chemotypes were identified by gas chromatography (GC). Association analyses between leaf color and chemotype were performed in segregating F2populations. Genes involved in leaf color formation and PL biosynthesis were mapped onto the published Hoko-3 reference genome to provide genomic evidence for the genetic relationship between the two traits. All F1individuals were uniformly PA-type. The three F2populations exhibited distinct leaf color–chemotype association patterns: Z01 (n= 118) showed a strong association (Fisher’s exactp= 9.13 × 10−10; φ = 0.564), Z02 (n= 117) showed no detectable association (p= 0.9; φ = 0.012), and Z03 (n= 88) showed a moderate association (p= 0.00669; φ = 0.289). Importantly, purple-leaf PL-type recombinants were obtained in F2populations and stably maintained through subsequent generations (F3–F5), demonstrating that the PL-type trait is not tightly linked with the green-leaf trait inP. frutescens.Genomic mapping genes related to leaf color and PL biosynthesis are distributed across multiple chromosomes and usually present as multiple loci, which is consistent with the pattern of incomplete linkage. The PL-type trait is recessive and not genetically tightly linked to the green-leaf traits inP. frutescens. The successful creation of a purple-leaf PL-type germplasm breaks the historical phenotypic constraint and provides a novel material for further dissection of the molecular mechanisms regulating secondary metabolism and organ coloration inP. frutescens.
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产品关联
DOI
高影响因子
Plant Methods
·
2023
·Vol. 19
·期 1
·1-17
·
IF 5.1
Jiang Zheng,Zhou Peina,Shao Yongfang,Zhang Qianqian,Yue Wei,Qu Cheng,Wu Qinan
Background Glandular trichomes, often referred to as “phytochemical factories”, plays a crucial role in plant growth and metabolism. As the site for secretion and storage, the development of glandular trichomes is related to the dynamic biosynthesis of specialised metabolites. The study aims to explore the relationship between spatial phenotype and dynamic metabolism of glandular trichomes, and establish a novel approach for the exploration and study of the regulatory mechanism governing the development of glandular trichomes. Results In this study, we proposed a technical route based on the relative deviation value to distinguish the peltate glandular trichomes (PGTs) from the background tissues and extract their spatial phenotype. By defining glandular trichome developmental stages based on the leaf vein growth axis, we found that young PGTs were densely distributed near the proximal end of growth axis of the leaf veins, where perillaketone, a primary metabolite of PGTs, is predominantly accumulated. Conversely, mature PGTs are typically found near the distal end of the mid-vein growth axis and the lateral end of the secondary vein growth axis, where the accumulation rate of isoegomaketone and egomaketone exceeds that of perillaketone in PGTs. We further identified spatial phenotypic parameters, L sum and d, as independent variables to construct a linear regression model that illustrates the relationship between the spatial phenotypes and metabolite content of PGTs, including perillaketone (R 2 = 0.698), egomaketone (R 2 = 0.593), isoegomaketone (R 2 = 0.662) and the sum of the amount (R 2 = 0.773). Conclusions This model proved that the development of PGTs was correlated with the growth of the entire leaf, and the development stage of PGTs can be identifined by spatial phenotypes based on the leaf veins. In conclusion, the findings of this study enhance our understanding of correlation between spatial phenotype and development of glandular trichomes and offer a new approach to explore and study the regulatory mechanism of glandular trichome development.
研究领域:
植物学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Chunte Feng,Hongfei Gao,Yang Yang,Xinyu Yang,Hao Tian,Huiyan Gu,Lei Yang
In this study, an acid-resistant magnetic boron-modified molecularly imprinted polymer (Fe 3 O 4 @NH 2 @BA@Lyz-MIPs) was prepared by the phase transition of lysozyme and used for the selective recognition and separation of rosmarinic acid from Perilla frutescens leaf extract. The synthesized materials were characterized by thermogravimetric analysis (TG), infrared spectroscopy (FT-IR), electron microscopy (SEM), and vibrating sample magnetometry (VSM). The adsorption and desorption experiments, adsorption kinetics experiments, and adsorption thermodynamics experiments revelated that the Fe 3 O 4 @NH 2 @BA@Lyz-MIPs exhibited a better adsorption capacity for rosmarinic acid (6.31 mg/g). Furthermore, Fe 3 O 4 @NH 2 @BA@Lyz-MIPs still exhibit a good adsorption performance after 8 adsorption-desorption cycles. The adsorption capacity decreased by 8.12 %, and the desorption performance decreased to 78.84 %. Stability experiments showed that the prepared imprinted layer was not destroyed by the eluent and could protect the internal magnetic core of the material. In summary, the prepared molecularly imprinted polymer Fe 3 O 4 @NH 2 @BA@Lyz-MIPs has good selective adsorption and high acid stability and can be swiftly retrieved from solution with the aid of an applied magnetic field, which provides a rapid and environmentally friendly strategy for the separation of rosmarinic acid from a complicated matrix.
研究领域:
分析化学
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材料科学
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药学
·
生物技术
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Kai-lin Chen,Dong-dong Xie,Man-ping Luo,Bing Liu,Yang Li,Yu-jie Zhao,Xiao-xiao Zhao,Jia-mei Pei,Yong-gang Ding,Zai-Ping Feng,Bei Wang,Xin-guo Zhang
Amid growing concerns regarding gout and hyperuricemia associated with high-protein and purine-rich diets, the need for effective prevention and management strategies with minimal side effects has become increasingly critical. This study evaluates the potential of three commonly consumed plant-based functional foods, Chrysanthemum morifolium, Perilla frutescens, and Sophora japonica, inhibiting xanthine oxidase (XO) and adenosine deaminase (ADA), key enzymes in uric acid metabolism. Results from hyperuricemia model mice indicate that this blend significantly reduces serum uric acid levels, mirroring the efficacy of conventional prevention and management strategies such as allopurinol but with fewer adverse effects. Liquid chromatography–mass spectrometry (LC-MS) analysis confirms that flavonoids are the primary bioactive agents, exhibiting a strong affinity for XO. These findings highlight the viability of integrating plant-based functional foods into comprehensive gout management strategies, underscoring their role in enhancing patient health through dietary innovation.
研究领域:
药理学
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营养学
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食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2021
·Vol. 10
·期 2
·315
·
IF 4.35
Zhenxing Wang,Zongcai Tu,Xing Xie,Hao Cui,Kin Weng Kong,Lu Zhang
This study aims to evaluate the bioactive components, in vitro bioactivities, and in vivo hypoglycemic effect ofP. frutescensleaf, which is a traditional medicine-food homology plant.P. frutescensmethanol crude extract and its fractions (petroleum ether, chloroform, ethyl acetate, n-butanol fractions, and aqueous phase residue) were prepared by ultrasound-enzyme assisted extraction and liquid–liquid extraction. Among the samples, the ethyl acetate fraction possessed the high total phenolic (440.48 μg GAE/mg DE) and flavonoid content (455.22 μg RE/mg DE), the best antioxidant activity (the DPPH radical, ABTS radical, and superoxide anion scavenging activity, and ferric reducing antioxidant power were 1.71, 1.14, 2.40, 1.29, and 2.4 times higher than that of control Vc, respectively), the most powerful α-glucosidase inhibitory ability with the IC50value of 190.03 μg/mL which was 2.2-folds higher than control acarbose, the strongest proliferative inhibitory ability against MCF-7 and HepG2 cell with the IC50values of 37.92 and 13.43 μg/mL, which were considerable with control cisplatin, as well as certain inhibition abilities on acetylcholinesterase and tyrosinase. HPLC analysis showed that the luteolin, rosmarinic acid, rutin, and catechin were the dominant components of the ethyl acetate fraction. Animal experiments further demonstrated that the ethyl acetate fraction could significantly decrease the serum glucose level, food, and water intake of streptozotocin-induced diabetic SD rats, increase the body weight, modulate their serum levels of TC, TG, HDL-C, and LDL-C, improve the histopathology and glycogen accumulation in liver and intestinal tissue. Taken together,P. frutescensleaf exhibits excellent hypoglycemic activity in vitro and in vivo, and could be exploited as a source of natural antidiabetic agent.Keywords:P. frutescensleaf;phytochemical composition;antioxidant activity;enzyme inhibitory;anticancer ability;antidiabetic activity
研究领域:
药理学
·
生物化学
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医学
·
植物科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）