Frontiers in Microbiology
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2025
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IF 4.5
Ting-Bin Hao,Peng-Yu Lai,Zhan Shu,Ran Liang,Zhi-Yun Chen,Ren-Long Huang,Yang Lu,Adili Alimujiang
Water scarcity is an escalating environmental concern. The model diatom, Phaeodactylum tricornutum, holds promise as a potential cell factory for the production of high-value natural compounds. However, its dependence on saline water cultivation restricts its use in areas facing water shortages. Although numerous studies have delved into the metabolic mechanisms of plants under water stress, there is a limited understanding when it comes to microalgae. In our study, we employed polyethylene glycol (PEG) to simulate water scarcity conditions, and assessed a range of parameters to elucidate the metabolic responses of P. tricornutum. Water stress induced the generation of reactive oxygen species (ROS), curtailed the photosynthetic growth rate, and amplified lipid content. Our insights shed light on the physiology of P. tricornutum when subjected to water stress, setting the stage for potential applications of microalgae biotechnology in regions grappling with water scarcity.
研究领域:
藻类生物学
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代谢组学
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DOI
PubMed
高影响因子
MARINE POLLUTION BULLETIN
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2019
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IF 3.782
Fang Dong,Xiaoshan Zhu,Wei Qian,Pu Wang,Jiangxin Wang
Ocean acidification (OA) and heavy metals are common stress factors for marine ecosystems subject to anthropogenic impacts. OA coupled with the heavy metal is likely to affect marine species. This study investigated the single and combined effects of OA (1500 ppm) and cadmium (Cd; 0.4, 1.2 mg/L) on the marine diatom Phaeodactylum tricornutum under 7 d exposure. The results clearly indicated that either OA or Cd stress (1.2 mg/L) alone inhibited the growth of P. tricornutum . However, under the combined OA-Cd stress, the growth inhibition disappeared, and the intracellular oxidative damage was mitigated. These results indicated a significantly enhanced tolerance of P. tricornutum to Cd while under OA conditions, which could be beneficial to the survival of this diatom. This study will ultimately help us understand the responses of marine organisms to multiple stressors and have broad implications for the potential ecological risks of Cd under future OA conditions.
研究领域:
海洋生物学
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环境科学
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DOI
PubMed
高影响因子
Biotechnology for Biofuels
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2018
·Vol. 11
·期 1
·1-14
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IF 5.497
Wang Xiang,Dong Hong-Po,Wei Wei,Balamurugan Srinivasan,Yang Wei-Dong,Liu Jie-Sheng,Li Hong-Ye
Background Metabolic engineering has emerged as a potential strategy for improving microalgal lipid content through targeted changes to lipid metabolic networks. However, the intricate nature of lipogenesis has impeded metabolic engineering. Therefore, it is very important to identify the crucial metabolic nodes and develop strategies to exploit multiple genes for transgenesis. In an attempt to unravel the microalgal triacylglycerol (TAG) pathway, we overexpressed two key lipogenic genes, glycerol-3-phosphate acyltransferase (GPAT1) and lysophosphatidic acid acyltransferase (LPAT1), in oleaginous Phaeodactylum tricornutum and determined their roles in microalgal lipogenesis. Results Engineered P. tricornutum strains showed enhanced growth and photosynthetic efficiency compared with that of the wild-type during the growth phase of the cultivation period. However, both the cell types reached stationary phase on day 7. Overexpression of GPAT1 and LPAT1 increased the TAG content by 2.3-fold under nitrogen-replete conditions without compromising cell growth, and they also orchestrated the expression of other key genes involved in TAG synthesis. The transgenic expression of GPAT1 and LPAT1 influenced the expression of malic enzyme and glucose 6-phosphate dehydrogenase, which enhanced the levels of lipogenic NADPH in the transgenic lines. In addition, GPAT1 and LPAT1 preferred C16 over C18 at the sn-2 position of the glycerol backbone. Conclusion Overexpression of GPAT1 together with LPAT1 significantly enhanced lipid content without affecting growth and photosynthetic efficiency, and they orchestrated the expression of other key photosynthetic and lipogenic genes. The lipid profile for elevated fatty acid content (C16-CoA) demonstrated the involvement of the prokaryotic TAG pathway in marine diatoms. The results suggested that engineering dual metabolic nodes should be possible in microalgal lipid metabolism. This study also provides the first demonstration of the role of the prokaryotic TAG biosynthetic pathway in lipid overproduction and indicates that the fatty acid profile can be tailored to improve lipid production.
研究领域:
微生物学
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生物技术
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代谢工程
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植物科学
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DOI
PubMed
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
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2025
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IF 1.6
Yuan Yijun,Li Jiawei,Zhang Sinan,Dong Ceyu,Tang Shun,Tu Zimeng,Liu Nana
Nuclear power stands as a linchpin in decarbonization efforts, primarily due to its dense energy output per unit and the virtual absence of emissions when operational. Exploiting the vast uranium reserves in the ocean is crucial for overcoming land resource scarcity and providing a sustainable, long-term solution. This research aimed to evaluate the adsorptive capacity of a novel marine-derived diatom species, Phaeodactylum tricornutum , for low-concentration uranium in simulated seawater. Biosorption results across varying experimental conditions demonstrate that Phaeodactylum tricornutum exhibits outstanding uranium adsorption capacity ( q max = 13.67 mg g −1 ). Additionally, Phaeodactylum tricornutum sustained exceptional uranium (U(VI)) selectivity and demonstrated favorable reusability in aqueous solution systems. A chemisorption process featuring uniform monolayer adsorption was corroborated by the pseudo-second-order and Langmuir model fittings. Thermodynamic analysis revealed that the adsorption process was spontaneous, endothermic, and characterized by increased randomness. The dominant adsorption mechanism was identified as coordination between uranyl ions and hydroxyl, carboxyl and amino groups. Owing to its abundant sources, easy accessibility, eco-friendliness and recyclability, the marine diatom Phaeodactylum tricornutum demonstrates significant promise for application in seawater uranium extraction.
研究领域:
海洋生物技术
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环境科学
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核能
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生物吸附
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水体化学
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DOI
高影响因子
iScience
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2024
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IF 4.6
Yixi Su,Jiwei Chen,Jingyan Hu,Cheng Qian,Jiahao Ma,Sigurður Brynjolfsson,Weiqi Fu
A major obstacle to exploiting industrial flue gas for microalgae cultivation is the unfavorable acidic environment. We previously identified three upregulated genes in the low-pH-adapted model diatom Phaeodactylum tricornutum : ferredoxin (PtFDX), cation/proton antiporter (PtCPA), and HCO 3 − transporter (PtSCL4-2). Here, we individually overexpressed these genes in P. tricornutum to investigate their respective roles in resisting acidic stress (pH 5.0). The genetic modifications enabled positive growths of transgenic strains under acidic stress that completely inhibited the growth of the wild-type strain. Physiological studies indicated improved photosynthesis and reduced oxidative stress in the transgenic strains. Transcriptomes of the PtCPA- and PtSCL4-2-overexpressing transgenics showed widespread upregulation of various transmembrane transporters, which could help counteract excessive external protons. This work highlights ion/electron carrier genes’ role in enhancing diatom resistance to acidic stress, providing insights into phytoplankton adaptation to ocean acidification and a strategy for biological carbon capture and industrial flue gas CO 2 utilization.
研究领域:
植物学
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遗传学
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环境科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2024
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IF 9.7
Hui-Ying Xu,Ruo-Yu Li,Zi-Qiang Yang,Jia-Fan Qiu,Yu-Bin Su,Carol Sze Ki Lin,Wei-Dong Yang,Hong-Ye Li,Jian-Wei Zheng,Xiang Wang
In this study, the biochemical response of Phaeodactylum tricornutum to varying concentrations of inorganic selenium (Se) was investigated. It was observed that, when combined with fulvic acid, P. tricornutum exhibited enhanced uptake and biotransformation of inorganic Se, as well as increased microalgal lipid biosynthesis. Notably, when subjected to moderate (5 and 10 mg/L) and high (20 and 40 mg/L) concentrations of selenite under fulvic acid treatment, there was a discernible redirection of carbon flux towards lipogenesis and protein biosynthesis from carbohydrates. In addition, the key parameters of microalgae-based biofuels aligned with the necessary criteria outlined in biofuel regulations. Furthermore, the Se removal capabilities of P. tricornutum , assisted by fulvic acid, were coupled with the accumulation of substantial amounts of organic Se, specifically SeCys. These findings present a viable and successful approach to establish a microalgae-based system for Se uptake and biotransformation.
研究领域:
海洋生物学
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环境科学
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生物化学
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生物技术
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2018
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IF 3.974
Benben Du,Zhenyan Zhang,Wanyue Liu,Yizhi Ye,Tao Lu,Zhigao Zhou,Yan Li,Zhanyu Fu,Haifeng Qian
Azoxystrobin (AZ) is an effective broad-spectrum fungicide . Due to its extensive application, AZ is detectable in aquatic ecosystems and thus influences aquatic organisms. In this study, the acute toxicity (96 h) of AZ at concentrations of 1.0 mg/L and 5.0 mg/L on the diatom Phaeodactylum tricornutum were examined. At the tested concentrations, AZ significantly inhibited P. tricornutum growth and destroyed its cellular structure. Furthermore, the mechanisms of AZ-induced toxicity on P. tricornutum changed as the exposure time extended. Forty-eight hours after exposure, AZ inhibited P. tricornutum growth primarily via inducing oxidative stress , which increased the activity of two main antioxidant enzymes, superoxide dismutase and peroxidase, and inhibited energy metabolism. However, after 96 h of treatment, the decline in the photosynthetic capacity of P. tricornutum demonstrated that the photosystem was the main AZ target. The pigment content and expression levels of genes related to photosynthetic electron transfer reactions were also significantly decreased. The present study describes AZ toxicity in P. tricornutum and is very valuable for assessing the environmental risk of AZ.
研究领域:
环境科学
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毒理学
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水生生态学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Qiufeng Song,Changhong Liu,Ran Xu,Luyun Cai
Diatoms exhibit great potential for biofuels, high value-added production from wastewater and promoting carbon neutrality. Fucoxanthin is identified as the main carotenoid in diatoms, which is a photosynthetic pigment with a variety of biological activities and physiological functions. Light quality is essential for microalgal growth, photosynthesis and intracellular metabolism, and microalgal cells often respond to the effects of light to regulate their biochemical makeup. Therefore, goal of this research was to develop a strategy for altering light quality and intensity to maximize fucoxanthin generation, in line with the photoresponsive traits of Phaeodactylum tricornutum and to investigate the underlying mechanism based on metabolomics and transcriptomics. Different light qualities, culture strategies, light intensities and light ratios were explored and optimized, and the findings illustrated that the optimal fucoxanthin production was observed when under the red light during the initial phase, followed by a transition to the blue-green mixed light (60μmol m −2 s −1 ) conditions in the subsequent phase. Transcriptome and metabolome analyses showed that the light shift strategy provided sufficient energy and precursors for cell growth and fucoxanthin accumulation by enhancing the EMP pathway, TCA cycle, and light-harvesting complex in photosynthetic system. The present study provides a feasible strategy and mechanism to customize the light quality regulatory strategy to enhance biosynthesis of target bioactive compounds, and provides a theoretical basis for further investigation of the regulatory mechanism, which can provide a foundation for microalgal fucoxanthin production.
研究领域:
微藻生物学
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生物化学
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环境科学
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DOI
高影响因子
Frontiers in Microbiology
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2022
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IF 6.064
Li Bing,Wu Di,Li Yan,Shi Yan,Wang Chenlin,Sun Jiasi,Song Chunfeng
Antibiotic resistance is one of the most important environmental challenges. Microalgae has been considered as a promising green media for environmental purification. In this work, sulfadimethoxine (SDM) biodegradation potential of Chlorella sp. L38 and Phaeodactylum tricornutum MASCC-0025 is investigated. Experimental results indicated that the tested freshwater and marine microalgae strains presented stress response to SDM addition. For Chlorella sp. L38, it has a good adaptability to SDM condition via antioxidant enzyme secretion (SOD, MDA, and CAT up to 23.27 U/mg, 21.99 μmol/g, and 0.31 nmol/min/mg) with removal rate around 88%. P. tricornutum MASCC-0025 exhibited 100% removal of 0.5 mg/L SDM. With increasing salinity (adding a certain amount of NaCl) of cultivation media, the removal rate of SDM by microalgae increased. Although its adaptive process was slower than Chlorella sp. L38, the salinity advantage would facilitate enzyme accumulation. It indicated that microalgae could be used to remove SDM from freshwater and marine environment via suitable microalgae strain screening.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
Biotechnology for Biofuels
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2020
·Vol. 13
·期 1
·1-14
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IF 4.815
Wang Xiang,Liu Si-Fen,Li Ruo-Yu,Yang Wei-Dong,Liu Jie-Sheng,Lin Carol Sze Ki,Balamurugan Srinivasan,Li Hong-Ye
Background Despite the great potential of marine diatoms in biofuel sector, commercially viable biofuel production from native diatom strain is impractical. Targeted engineering of TAG pathway represents a promising approach; however, recruitment of potential candidate has been regarded as critical. Here, we identified a glycerol-3-phosphate acyltransferase 2 (GPAT2) isoform and overexpressed in Phaeodactylum tricornutum.Results GPAT2 overexpression did not impair growth and photosynthesis. GPAT2 overexpression reduced carbohydrates and protein content, however, lipid content were significantly increased. Specifically, TAG content was notably increased by 2.9-fold than phospho- and glyco-lipids. GPAT2 overexpression elicited the push-and-pull strategy by increasing the abundance of substrates for the subsequent metabolic enzymes, thereby increased the expression of LPAAT and DGAT. Besides, GPAT2-mediated lipid overproduction coordinated the expression of NADPH biosynthetic genes. GPAT2 altered the fatty acid profile in TAGs with C16:0 as the predominant fatty acid moieties. We further investigated the impact of GPAT2 on conferring abiotic stress, which exhibited enhanced tolerance to hyposaline (70%) and chilling (10 ºC) conditions via altered fatty acid saturation level.Conclusion sCollectively, our results exemplified the critical role of GPAT2 in hyperaccumulating TAGs with altered fatty acid profile, which in turn uphold resistance to abiotic stress conditions.
研究领域:
海洋生物学
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合成生物学
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代谢工程
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DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
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2021
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IF 4.401
Xuehua Liu,Lijun Wang,Songcui Wu,Lu Zhou,Shan Gao,Xiujun Xie,Lepu Wang,Wenhui Gu,Guangce Wang
The formation of resting cells/spores in marine diatoms helps them to survive in environmental changes, whose sedimentation characteristics also drive enhanced carbon fluxes export to the deep ocean. However, the molecular mechanism underlying the transformation remains unclear. Based on the upregulation of ferritin during dormancy in microorganism and its' crucial role in coping with environmental stress, we constructed the ferritin overexpression and silence strains in model marine diatom, Phaeodactylum tricornutum (FTN-OE and FTN Si, respectively). The morphological transformation from fusiform to ovoid were observed among the FTN-OE strains but not the wide type and FTN-Si strains. These FTN-OE oval cells exhibited typical characteristics of resting cells and benthic adaptation, such as lower growth rates, low-light adaptation, silicified valves, the excretion of exopolymeric substances, upregulation of stress-resistant proteins and increased nitrate reserves. Furthermore, FTN-OE oval cells showed increased abscisic acid synthesis and signaling pathways in the transcriptome and proteome . And the abscisic acid signal was detected in FTN-OE oval cells exclusively (2.96 ng g −1 FW), but absent in the FTN Si, FTN-OE fusiform or WT cells. Overall, our results demonstrate that ferritin overexpression in marine diatom have a close relationship with morphological transformation from fusiform to ovoid morphotype , after which they enter a resting stage, which is accompanied by abscisic acid accumulation. This improves our understanding of the molecular processes in diatom resting cells/spores' formation and suggests that ferritin accumulation might improve the sequestration of photosynthetically fixed atmospheric CO 2 into the deep sea.
研究领域:
海洋生物学
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分子生物学
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DOI
高影响因子
Frontiers in Marine Science
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2026
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IF 3.4
Rui Shi,Wei Chen,Yujun Hu,Bingjian Chen,Xiaoxiao Yu,Hui Huang,Dan Wang,Shunxin Hu
IntroductionShort-chain chlorinated paraffins (SCCPs) have garnered increased attention due to higher persistence, bioaccumulation, long-distance transportation potential, and heightened toxicity to aquatic species and mammals.MethodsThis paper evaluated the toxic effects of SCCPs on P. tricornutum during a 96-hour acute exposure experiment, focusing on growth inhibition, photosynthetic parameters, and oxidative stress responses.ResultsResults showed that at the environmental concentration (0.001 mg/L), SCCPs exerted no significant effects on the growth and photosynthesis of P. tricornutum. However, higher concentrations (2, 4, and 8 mg/L) exposure resulted in a pronounced, dose-dependent growth inhibition, with inhibition rates reaching 37.43%, 53.25%, and 54.47% (p < 0.05), respectively. SCCPs stress disrupted the photosynthetic system, leading to reduction in the contents of chlorophyll a, chlorophyll c and carotenoids. Key photosynthetic pigments were depleted, and Photosystem II (PSII) efficiency (Fv/Fm, Y(II), rETRmax) was markedly suppressed. Furthermore, SCCPs exposure induced oxidative damage, as evidenced by elevated reactive oxygen species (ROS) and malondialdehyde (MDA) levels. Mitochondrial damage led to insufficient energy supply, resulting in decreased antioxidant enzyme activity.DiscussionCollectively, these findings establish that high concentrations of SCCPs inhibit algal growth and photosynthesis through a dual mechanism involving direct photosynthetic disruption and the induction of oxidative damage.
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DOI
高影响因子
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