SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Jing Wang,Tingting Li,Haigang Li,Gongke Li,Shixin Wu,Liansheng Ling
AuNPs-DNA probes remain a challenge to recognize the double-stranded DNA (dsDNA) from routine PCR product despite its success in the recognition of the single-stranded (ssDNA) DNA of asymmetry-PCR product. Here, a universal colorimetric PCR biosensor is established with oligonucleotide modified AuNPs by recognizing the dsDNA of PCR product based on triplex DNA formation with the assistance of a triplex DNA intercalator Ru(phen) 2 dppx 2+ . The strategy was applied to detect different length dsDNA, including short dsDNA 1 (49 bp) and long dsDNA 2 (321 bp), both of which had the good linear relationship between the absorbance and the logarithm of the concentration of the targets. Moreover, the limit of detection (LOD) for short and long length dsDNA was 0.5 pM and 0.05 fg, lower than those of the previously reported methods, respectively. This strategy also displayed high selectivity against dsDNA 1 and dsDNA 2. These results demonstrate a potential strategy to detect all length of dsDNA, providing new opportunities in the life science, diagnosis, and medicine.
研究领域:
生物化学
·
分子诊断
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
Energy Storage Materials
·
2024
·
IF 18.9
Dengxu Wu,Jian Peng,Zhiwen Jiang,Lei Zhu,Yujing Wu,Chang Xu,Zhixuan Wang,Ming Yang,Hong Li,Liquan Chen,Fan Wu
Lithium-metal batteries with solid electrolytes (SEs) have emerged as promising electrochemical energy storage devices due to high energy density and safety. However , inherent challenges of deleterious lithium dendrite growth and poor interfacial stability hinder their practical application. Herein , a new type of room-temperature liquidous Li metal arene complex—Li-Phenanthrene (Phen)-Ether is explored to enable a 3D LiSi@Li-Phen-Ether (3D LSLL) anode with low cost, high safety and high ion/electron-conductivity. Symmetric cells with sulfide SE and 3D LSLL anode deliver an ultra-high critical current density (> 13 mA cm −2 ) and long cycle life (> 1000 h, 0.25 mA cm −2 ). Moreover, full battery (LFP/sulfide SE/3D LSLL) can operate at low-external pressure (0.5 MPa) and ambient temperature by introducing a flexible cathode/sulfide interlayer with high ion conductivity (2 mS cm −1 ). This unprecedented battery configuration demonstrates high-rate (2C) performance and long cycle life (over 300 cycles), which exceeds preciously-reported sulfide SE/lithium batteries at low stack pressures, and may open up a promising route for high-energy-density, cost-effective and safe rechargeable lithium batteries.
研究领域:
能量存储
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材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
NANOTECHNOLOGY
·
2022
·Vol. 33
·期 21
·215502
·
IF 3.953
Fang-hui Wu,Guo-qiang Wen,Xiang-rui Luo,Xu-dong Xu,Zi Liu,Wen-bin Sun,Yan-shang Kang,Zhengquan Yan
To improve the sensitivity for electro-chemiluminescent (ECL) detection of chloramphenicol (CAP), a common broad-spectrum antibiotic, boron nitride quantum dots (BNQDs) were prepared with excellent photoelectric property and low toxicity. After its structure and electrochemical property were investigated in detail, it was noted that the ECL signal of Ru(Phen)32+ could be strengthened by the proposed BNQDs, which was further activated by ten's times in the presence of CAP. Under the optimized conditions, there was an excellent linear relationship between ΔECL and lgcCAP in a wide linear range from 1.0 × 10−10 to 1.0 × 10−6 mol l−1 CAP. The detection limit was super-low to be 3.3 × 10−11 mol l−1 (S/N = 3). When applied for CAP detection in real pharmaceutical and food samples, the recoveries were between 97.8% and 105.7% with R.S.D. less than 3.3%. A possible CAP-activated ECL mechanism of BNQDs-Ru(phen)32+ was also proposed. This work will offer a great potential for efficient monitoring of CAP pollution and clinical diagnosing of CAP-related diseases in future.
研究领域:
分析化学
·
环境科学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Bofan Li,Xiaoming Zhou,Hongxing Liu,Huaping Deng,Ru Huang,Da Xing
Antibiotic resistance, the ability of some bacteria to resist antibiotic drugs, has been a major global health burden due to the extensive use of antibiotic agents. Antibiotic resistance is encoded via particular genes; hence the specific detection of these genes is necessary for diagnosis and treatment of antibiotic resistant cases. Conventional methods for monitoring antibiotic resistance genes require the sample to be transported to a central laboratory for tedious and sophisticated tests, which is grueling and time-consuming. We developed a paper-based chip, integrated with loop-mediated isothermal amplification (LAMP) and the “light switch” molecule [Ru(phen)2dppz]2+, to conduct turn-on fluorescent detection of antibiotic resistance genes. In this assay, the amplification reagents can be embedded into test spots of the chip in advance, thus simplifying the detection procedure. [Ru(phen)2dppz]2+ was applied to intercalate into amplicons for product analysis, enabling this assay to be operated in a wash-free format. The paper-based detection device exhibited a limit of detection (LOD) as few as 100 copies for antibiotic resistance genes. Meanwhile, it could detect antibiotic resistance genes from various bacteria. Noticeably, the approach can be applied to other genes besides antibiotic resistance genes by simply changing the LAMP primers. Therefore, this paper-based chip has the potential for point-of-care (POC) applications to detect various gene samples, especially in resource-limited conditions.
研究领域:
生物技术
·
分子生物学
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医学诊断
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2022
·
IF 3.841
Yingling Zhong,Yuandong Wu,Dajiang Mei,Shaoguo Wen,Thomas Doert
Three 1,10-phenantroline zinc halide complexes were successfully synthesized under solvothermal method and structurally characterized by single-crystal X-ray diffraction analysis. The three title complexes Zn(1,10-phen)I 2 ( 1 ), Zn(1,10-phen) 2 I 2 ( 2 ) and Zn 2 (1,10-phen) 2 C 2 O 4 Br 2 ( 3 ), crystallize in the triclinic space group P 1 ¯ (No.2) with different unit cell parameters, are isolated neutral molecules. The extra stretching force from another 1,10-phen ligand in 2 lead to the Zn−N bond lengths longer than the corresponding in 1 . Meanwhile, the molecules of Zn(1,10-phen)I 2 and Zn(1,10-phen) 2 I 2 further connect each other via the π-π interactions between the adjacent phenanthrolines to form a pseudo-two-dimensional layer which parallel to b-axis. Their face-to-face distance of π-π stacking interactions between the adjoining 1,10-phen rings is 3.7264 Å and 3.4435 Å, respectively. The zinc bromide complex 3 contains C−H···O intramolecular and C−H···Br (2-D) interlayer hydrogen bonding, which contributed to the connection between the parallel zig-zag molecules. The distance of phenanthroline rings in intramolecular and interlayer is 2.6071 Å and 3.8125 Å. The luminescent emissions of free ligand 1,10-phen and its zinc halide complexes were measured in various solvents. These zinc halide complexes show the obvious luminescent phenomenon with grass-green, yellow-green and light yellow in Dimethyl sulfoxide (DMSO), respectively (the maximum emission wavelength is 530 nm, 539 nm and 496 nm). The maximum emission wavelength for 2 has a little red-shifted compared with 1 . It can be concluded that the number of ligand and the electron density on coordinated Zn atoms influence the emission wavelength through charge transfer from ligand to the metal. The emission wavelengths of 3 are also slightly red-shifted compared with the free 1,10-phen ligand, which is attributed to the coordination effects of the 1,10-phen ligands to the Zn cations and [C 2 O 4 ] 2− group.
研究领域:
化学
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材料科学
·
晶体学
✓ 已验证
产品关联
DOI
高影响因子
INTEGRATED FERROELECTRICS
·
2021
·
IF 0.836
Peisong Tang,Xuezhi Wei,Liqin Zhang,Ruipeng Hou,Yifan Zhou
Using Eu2O3 as source of tritium, anhydrous ethanol as solvent system, three β-diketone compounds of 2-thienyl trifluoroacetone (TTA), acetylacetone (Hacac), 1-(4-Methoxyphenyl)- 3-(4-tert-butylphenyl)-1,3-propanedione (Avobenzone) as first ligand and 1,10-phenanthroline (Phen) as second ligand, the three kinds europium (III) ternary complexes were prepared by precipitation method at 60 °C. The complexes were characterized by fluorescence spectroscopy (FS), Fourier transform infrared spectroscopy (FT-IR), thermogravimetry and differential scanning calorimetry (TG-DSC) and color coordinates. The results show that three red light-emitting europium (III) ternary complexes can emit strong red characteristic fluorescence. Thereinto, Eu(TTA)3Phen has the best luminescent performance. The excitation spectrum of Eu(TTA)3Phen is 395 nm, the characteristic emission peak is at 612 nm, and the color coordinate is (0.66, 0.34). Eu(TTA)3Phen show good thermal stability which is decomposed more than 200 °C.
研究领域:
材料科学
·
光学
✓ 已验证
DOI
高影响因子
DYES AND PIGMENTS
·
2023
·
IF 4.5
Runze Jia,Xianju Zhou,Jian Wu,Li Li,Yongjie Wang,Guotao Xiang,Xiantong Tang,Zhiyu Yang,Huajing Gao
Stimuli-responsive smart luminescent materials have attracted more and more attention recently. A series of lanthanide organic complexes, [Ln 2 (BPDC) 3 (Phen) 2 (DMF) 2 ]·5H 2 O (Ln: lanthanide = Eu, Tb, Eu x Tb 1−x , H 2 BPDC = 2,2′-Bipyridine-5,5′-dicarboxylic acid, H 2 Phen = 1,10-phenanthroline, DMF = N,N-dimethylformamide), were synthesized and characterized to be iso-structural. The complexes demonstrate selective and significant response to Fe 3+ ions, leading to fast and accurate measuring of Fe 3+ ions. Interestingly, the complexes exhibit diverse luminescent response to two isomers 2, 6-pyridine dicarboxylic acid (DPA) and 3, 5-pyridine dicarboxylic acid (H 2 PDC). The green emission due to Tb 3+ ions is enhanced by DPA upon excitation of 280 nm, which is ascribed to the absorption of DPA. Whilst it is quenched by DPA with the excitation of the ligands (339 nm). However, H 2 PDC quenches both the green and red emissions regardless of the excitation wavelength. The reason is that DPA provides more possibilities of the ligand exchange with the complex due to the suitable position of the carboxylic acid functional groups on the pyridine ring than H 2 PDC. Simultaneously, the luminescent response of the complex to DPA enables emission color tunability, which allows the complex to recognize and measure DPA/H 2 PDC isomers visually and easily. Moreover, the complex exhibits a wide-range (110–360 K) temperature sensing with high Sr = 3.92 %K −1 at 180 K. The multi-stimuli-response of the complexes makes them potential applicants for multi-functional sensing materials.
研究领域:
化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS A-PHYSICAL
·
2021
·
IF 3.407
Xiaoying Pan,Jun Hu,Jialiang Li,Yufei Zhai,Song Li,Min Wang,Takashiro Tsukamoto,Shuji Tanaka
The temperature dependent photoluminescence of temperature sensitive paint (TSP) makes it an attractive candidate for infrared (IR) thermal imaging, but its low luminescence intensity and poor photostability hinder its extensive use in infrared detectors . Herein, a novel complex luminophore of AgNPs/[Ru(phen) 3 ]Cl 2 /PVDF was designed to fabricate a thermal imaging device with improved sensitivity and signal-to-noise ratio. It is found that the localized surface plasmon resonance (LSPR) of silver nanoparticles (AgNPs) could enhance the local intensity of the excitation light , consequently an increase in the luminescence intensity of neighboring luminophore can be achieved. Thus, not only the fluorescence intensity of the obtained sensor array is enhanced, but the temperature sensitivity is also improved to − 4.5%/K at 20 °C, which increased by 25% compared to that of the blank film without AgNPs addition. Furthermore, the detector array is used for IR thermal imaging, which can successfully obtain the thermal images of observation object at room temperature . The minimal detectable temperature is as small as about 313 K.
研究领域:
材料科学
·
光学
·
热工程
·
纳米技术
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Zixuan Zhou,Yuhan Jiang,Xin Luo,Ying Zhang,Lei Wang,Yaguang Sun,Mingchang Zhu,Shuangyan Wu
The development of efficient, rapid, portable, and accurate analysis methods for detecting veterinary antibiotic residues in water matrices is highly demanded for environmental safety assessment. In this study, we have developed a sensor array (Cd-phen-PEDA) for the discrimination of various nitroimidazole antibiotics (NIIMs). The Cd-phen-PEDA sensor array exhibits high selectivity and sensitivity for the detection of metronidazole (MNZ), ornidazole (ONZ), and dimetridazole (DMZ). The limit of detection (LOD) for each antibiotic is 6.18 μM for MNZ, 4.80 μM for ONZ, and 5.40 μM for DMZ. The detection application of the Cd-phen-PEDA sensor array for nitroimidazole antibiotics (NIIMs) was successfully extended to real environmental samples. This demonstrates the practical applicability and reliability of the sensor in real-world scenarios, further enhancing its potential for environmental safety assessment. The Linear Discriminant Analysis (LDA) and hierarchical cluster analysis (HCA) results confirm the effectiveness of the Cd-phen-PEDA-based luminescent array in simultaneously identifying and detecting nitroimidazole antibiotics (NIIMs) in environmental samples. This dual analytical approach not only enhances the accuracy of detection but also provides a robust method for the discrimination and quantification of these antibiotics across various environmental matrices.
研究领域:
分析化学
·
环境科学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
POLYHEDRON
·
2025
·
IF 2.6
Keliang Wan,Lifeng Tan
Double-stranded RNA determines and regulates many biological processes in cells, plays a vital role in cell information transmission and gene regulation, and is a key target for the treatment of certain diseases. Here, we synthesized and characterized two novel ruthenium(II) complexes [Ru(phen) 2 ( o -CNPIP)] 2+ (Ru 1 ) and [Ru(phen) 2 ( o -CH 3 PIP)] 2+ (Ru 2 , phen = 1,10-phenanthroline, o -CNPIP = (2-(4-cyanodipyrido)-imidazo-[4,5- f ][1,10]-phenanthroline, o -CH 3 PIP = 2-(4-methyl)-imidazo-[4,5- f ][1,10]-phenanthroline). The interaction of the two Ru (II) complexes with double-stranded RNA poly(rA)•poly(rU) under dilute solution and molecular crowding conditions was studied by various spectroscopic methods and viscosity measurements. The results of UV–vis absorption spectroscopy and steady-state emission spectroscopy studies show that Ru 1 showed stronger binding and stabilizing ability to poly(rA)•poly(rU) and the binding affinity of Ru 1 and Ru 2 to poly(rA)•poly(rU) was weakened under molecular crowding conditions. Subsequently, the results of circular dichroism spectroscopy studies, thermal denaturation measurements and viscosity measurements also confirmed the results of our UV–vis absorption spectroscopy and steady-state emission spectroscopy studies. The results also showed that the two Ru(II) complexes bind, stabilize and perturb the structure of poly(rA)•poly(rU) by intercalation under dilute solution and molecular crowding conditions. In addition, it was found that the strength of these abilities was closely related to the binding affinity of the Ru(II) complexes to poly(rA)•poly(rU) by comparing the results of these studies. Furthermore, the thermodynamic parameters for the binding of Ru(II) complexes to poly(rA)•poly(rU) under the two conditions indicate that the binding process is spontaneous, is an enthalpy-driven process, and that hydrogen bonding serves as the dominant force. The purpose of this work is to explore the substituent effect of Ru(II) complexes and the effect of solution environment on the ability of Ru(II) complexes to bind and stabilize poly(rA)•poly(rU). Some insights resulting from this work are expected to facilitate the design of highly selective double-stranded RNA binders based on Ru(II) complexes. Interestingly, the phosphorescence intensity of Ru 2 after binding to poly(rA)•poly(rU) is significantly higher than that of Ru 1 under both solution conditions, with even stronger phosphorescence emission observed under molecular crowding conditions. This indicates that Ru 2 possesses the potential to serve as a nucleic acid luminescent probe for nucleic acid imaging in biological cells. To enhance its luminescent properties and specific binding capacity, further structural modification of Ru 2 is essential, which will be one of the key focuses of our future research efforts.
研究领域:
无机化学
·
生物无机化学
·
核酸化学
·
分子光谱学
·
生物物理化学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 57
·51591-51597
·
IF 3.289
Dongdong Lu,Fangyuan Gai,Zhen-An Qiao,Xue Wang,Tao Wang,Yunling Liu,Qisheng Huo
This work demonstrated that a series of ultrabright fluorescent silica nanoparticles have been synthesized and designed via the reverse microemulsion method to encapsulate (2,2′-bipyridine)2-(5-aminophenanthroline) Ru bis(hexafluorophosphate) (Rubpy-phen). Transmission electron microscopy (TEM), scanning electron microscopy (SEM), dynamic light scattering (DLS), inductively coupled plasma (ICP) and fluorescence spectra were used to study the morphology and properties of the nanoparticles. By introducing ethanol into the reaction system, the obtained uniform fluorescent nanoparticles showed monodispersity and controllable size. With an increase of nanoparticle size (from 50–180 nm), the relative brightness of the nanoparticles was greatly improved from 534 to 86 972 by comparison with free dye molecules. Due to the protection of the silica matrix, the fluorescence of high concentration dye-doped silica nanoparticles (Rubpy-phen NPs) is not quenched, which results in higher fluorescence intensity and relative brightness. Moreover, the silica matrix can also protect Rubpy-phen from leakage. Due to the high brightness and biocompatibility, Rubpy-phen NPs have potential applications in the biological field.
研究领域:
材料科学
·
生物学
·
纳米医学
✓ 已验证
DOI
高影响因子
INORGANIC CHEMISTRY
·
2022
·
IF 5.436
Feng Guo,Duo-Fu Li,Fang Gao,Kai Xu,Jun Zhang,Xiu-Guang Yi,Dong-Ping Li,Yong-Xiu Li
Luminescent lanthanide cages have many potential applications in guest recognition, sensing, magnetic resonance imaging (MRI), and bioimaging. However, these polynuclear lanthanide assemblies’ poor stability, dispersity, and luminescence properties have significantly constrained their practical applications. Furthermore, it is still a huge challenge to simultaneously synthesize and design lanthanide organic polyhedra with high stability and quantum yield. Herein, we demonstrate a simple and robust strategy to improve the rigidity, chemical stability, and luminescence of an Eu(III) tetrahedral cage by introducing the conjugated planar auxiliary phen ligand. The self-assembled tetrahedral cage, (Eu4L4)(phen)4 [L = (4,4′,4″-tris(4,4,4-trifluoro-1,3-dioxobutyl)-triphenylamine), phen = 1,10-phenanthroline], exhibited characteristic luminescence of Eu3+ ions with high quantum yield (41%) and long lifetime (131 μs) in toluene (1.0 × 10–6 M). Moreover, the Eu(III) cage was stable in water and even in an aqueous solution with a pH range of 1–14. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cellular imaging revealed that the Pluronic F127-coated hybrid material, (Eu4L4)(phen)4@F127, exhibited low cytotoxicity, good biocompatibility, and cellular imaging ability, which may inspire more insights into the development of lanthanide organic polyhedra (LOPs) for potential biomedical applications.
研究领域:
化学
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）