OPTICAL MATERIALS
·
2020
·
IF 2.779
Lu Chen,Fang Lei,Haohong Chen,Luqiao Yin,Ding Zhou,Ying Shi,Teng Ma
A novel Eu 3+ doped SiO 2 -B 2 O 3 -Al 2 O 3 -CaO-Na 2 O based borosilicate glass was designed to embed YAG: Ce for PiGs. It exhibits intense Eu 3+ red emission with the average decay times of 6.8 μs and has the thermal conductivity of about 0.9 W/(mK) (five times that of silicone resin). The doped Eu 3+ is over 10 mol% without any concentration quenching. The PiGs show variable decay time of Ce 3+ , which results from the tunable interface defects explained by the pseudo-solid solubility mechanism. The color of the WLEDs changes from cool white (6004 K) to warm white (3799 K) with more yellow emission added. The obtained maximal luminous efficiency and color rendering index are about 68 lm/W and 78, respectively. The anti-thermal quenching power of the PiGs is three times that of PiS based on the luminescent reduction. The PiG system can be used potentially for resin-free high power WLEDs.
研究领域:
材料科学
·
光学工程
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DOI
高影响因子
Journal of Veterinary Science
·
2017
·Vol. 18
·期 4
·431-437
·
IF 1.164
Ling Zhang,Li Zhao,Yonghong Liu,Junfeng Liu,Xianqiang Li
A comparative in vivo pharmacokinetic (PK) study of tilmicosin (TIL) was conducted in 6 crossbred healthy pigs and 6 crossbred pigs infected with Haemophilus ( H .) parasuis following oral administration of a single 40 mg/kg dose. The infected model was established by intranasal inoculation and confirmed by clinical signs, blood biochemistry, and microscopic examinations. Plasma TIL concentrations were determined by a validated high-performance liquid chromatography method with ultraviolet detection at 285 nm. PK parameters were calculated by using WinNonlin software. After TIL administration, the main PK parameters of TIL in healthy and H. parasuis -infected pigs were as follows: Area under the concentration-time curve, maximal drug concentration, half-life of the absorption phase, half-life of the distribution phase, and half-life of the elimination phase were 34.86 ± 9.69 vs . 28.73 ± 6.18 µg · h/mL, 1.77 ± 0.33 vs . 1.67 ± 0.28 µg/mL, 2.27 ± 0.45 vs . 2.24 ± 0.44 h, 5.35 ± 1.40 vs . 4.61 ± 0.35 h, and 43.53 ± 8.17 vs . 42.05 ± 9.36 h, respectively. These results of this exploratory study suggest that there were no significant differences between the PK profiles of TIL in the healthy and H. parasuis -infected pigs.
研究领域:
兽医学
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药理学
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DOI
PubMed
高影响因子
Biomolecules
·
2019
·Vol. 9
·期 9
·486
·
IF 4.694
Xuan Li,Mingyu Mao,Yanan Zhang,Kaifan Yu,Weiyun Zhu
Succinate is a metabolic intermediate of the tricarboxylic acid (TCA) cycle in all aerobic organisms, and is also a vital microbial metabolite in the gut. Although succinate is known to regulate intestinal metabolism and immune function, its role in the protection of the intestinal epithelial barrier function and inflammation is poorly characterized. In this study, we evaluated the effects of succinate on intestinal epithelial barrier function and inflammation in pigs. Twenty-four growing pigs were distributed into three groups (n= 8) and received either a basal diet (control group) or the same diet supplemented with 0.1% succinate or 1% succinate. The diet supplemented with 1% succinate led to alterations in the intestinal morphology. We confirmed in vitro that 5 mM succinate treatment modulated intestinal epithelial permeability by increased transepithelial electrical resistance (TEER) in intestinal porcine epithelial cell (IPEC)-J2 cells. Furthermore, succinate treatment increased the abundance of tight junction proteins claudin-1, zona occluden (ZO)-1, and ZO-2 in the jejunum in vivo and in vitro. In addition, dietary succinate supplementation promoted the expression of inflammatory cytokines interleukin (IL)-25, IL-10, IL-8, and IL-18 in the jejunum. Taken together, these data identify a novel role of succinate in the modulation of intestinal epithelial barrier function, which may be a nutritional target to improve gut health in animals.Keywords:succinate;intestinal epithelial barrier;intestinal inflammation;pigs
研究领域:
营养学
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免疫学
·
动物科学
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DOI
PubMed
高影响因子
Bioengineering-Basel
·
2025
·Vol. 12
·期 11
·1164
·
IF 3.7
Liping Liu,Xinhai Mo,Run Guo,Fang Chen,Fan Ding,Gang Zhao,Bo Zhang
Background: Acute cholecystitis (AC) is characterized by gallbladder inflammation and is commonly accompanied by disordered gallbladder motility. Although laparoscopic cholecystectomy is the standard treatment, it carries procedure-related risks. Low-intensity pulsed ultrasound (LIPUS), a safe and noninvasive modality widely applied for muscle repair, may offer therapeutic benefits for AC-associated motility dysfunction. Methods: In vivo, LIPUS (0.5 W/cm2) was applied for 15 min daily to acute cholecystitis guinea pigs over three consecutive days, starting 24 h after reversing common bile duct ligation (CBDL) surgery. In vitro, LIPUS (0.5 W/cm2) was delivered for 5 min to isolated gallbladder muscle strips and for 30 s to isolated interstitial cells of Cajal (ICCs). Gallbladder function and histology were assessed in vitro and in vivo using immunofluorescence, Western blotting, calcium imaging, muscle strip contractility testing, and related molecular methods. Results: LIPUS increased intracellular Ca2+by activating the Ano1 channel in ICCs, thereby enhancing gallbladder smooth muscle contractility. At 72 h, the LIPUS 72 h (+) group showed a 71.3% increase in gallbladder muscle tone (p= 0.0001) and a 40.7% reduction in inflammation scores (p= 0.0001) compared with the LIPUS 72 h (−) group. Conclusions: LIPUS alleviates gallbladder contractile dysfunction in acute cholecystitis by acting on ICCs through mechanisms involving the promotion of ICC recovery and a reduction in inflammation.
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DOI
PubMed
高影响因子
PLoS One
·
2021
·Vol. 16
·期 4
·e0250423
·
IF 3.24
Xuejing Li,Yan Hui,Bingfeng Leng,Junli Ren,Yanni Song,Lianqiang Che,Xi Peng,Baojia Huang,Songling Liu,Lin Li,Dennis Sandris Nielsen,Yong Li,Xiaoshuang Dai,Shancen Zhao
The tight association between malnutrition and gut microbiota (GM) dysbiosis enables microbiota-targeting intervention to be a promising strategy. Thus, we used a malnourished pig model to investigate the host response and GM alterations under different diet supplementation strategies. Pigs at age of 4 weeks were fed with pure maize diet to induce malnutrition symptoms, and followed by continuous feeding with maize (Maize, n = 8) or re-feeding using either corn-soy-blend (CSB+, n = 10) or millet-soy-blend based (MSB+, n = 10) supplementary food for 3 weeks. Meanwhile, 8 pigs were fed on a standard formulated ration as control (Ref). The effect of nutritional supplementation was assessed by the growth status, blood chemistry, gastrointestinal pathology, mucosal microbiota composition and colon production of short-chain fatty acids. Compared with purely maize-fed pigs, both CSB+ and MSB+ elevated the concentrations of total protein and globulin in blood. These pigs still showed most malnutrition symptoms after the food intervention period. MSB+ had superior influence on the GM development, exhibiting better performance in both structural and functional aspects. MSB+ pigs were colonized by less Proteobacteria but more Bacteroidetes , Firmicutes and Lachnospira spp. Pearson’s correlation analysis indicated a strong correlation between the abundance of mucosal e.g., Faecalibacterium and Lachnospira spp. and body weight, crown-rump length and total serum protein. In conclusion, the malnutrition symptoms were accompanied by an aberrant GM, and millet-based nutritional supplementation showed promising potentials to restore the reduced GM diversity implicated in pig malnutrition.
研究领域:
动物营养
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微生物学
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胃肠病学
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营养科学
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DOI
PubMed
高影响因子
iScience
·
2023
·Vol. 26
·期 5
·106558-106558
·
IF 5.8
Xuelian Zhang,Ming Li,Wenjie Li,Linxiu Yue,Taiyuan Zhang,Qi Tang,Naidan Zhang,Xianyong Lan,Chuanying Pan
Chlorpyrifos (CPF) has been shown to have male reproductive toxicity in mice and rat. However, the association of CPF and male reproduction in pigs remains unknown. Therefore, this study attempts to investigate the damage of CPF on male reproduction in pigs and its potential molecular mechanisms. First, ST cells and porcine sperms were treated with CPF and then cell proliferation, apoptosis, motility of sperm, and oxidative stress levels were examined, respectively. Meanwhile, RNA sequencing was performed on ST cells before and after the treatment of CPF. The results of experiments in vitro showed that CPF had broad-spectrum toxic effects on ST cells and porcine sperms. The RNA-sequencing data and WB results indicated that CPF may regulate cell survival through the PI3K-AKT pathway. In conclusion, this study may lay the foundation for improving male fertility in pigs and provide theoretical information for human infertility.
研究领域:
生殖毒理学
·
分子生物学
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动物科学
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DOI
PubMed
高影响因子
REPRODUCTION IN DOMESTIC ANIMALS
·
2019
·Vol. 54
·期 6
·864-872
·
IF 1.638
Dan Song,Guoyun Wu,Quanwei Wei,Fangxiong Shi
Contents Bisphenol A (BPA) is a chemical of high production volume that is used widely in many industries and is known as a xenooestrogen and anti-thyroid hormone endocrine disrupter. There is little information regarding the effects of BPA in the presence of thyroid hormone on porcine granulosa cell development. Thus, the primary granulosa cells were treated with thyroxine (T4, 10 nM), BPA (10 µM) or T4 plus BPA; we subsequently evaluated the effects of T4 or BPA on 17β-estradiol synthesis, cellular proliferation and apoptosis. Our data showed that BPA significantly increased the accumulation of 17β-estradiol and promoted granulosa cell proliferation, whereas T4 significantly decreased 17β-estradiol and had no effect on cellular proliferation. In addition, it was noteworthy that T4 treatment induced apoptosis in porcine granulosa cells and BPA co-incubation attenuated T4-induced apoptosis as shown from flow cytometric assay analysis. We hypothesized that BPA attenuates T4-induced apoptosis by regulating 17β-estradiol accumulation and oestrogen receptor-mediated signalling pathways. In conclusion, our results demonstrated that T4 affected 17β-estradiol accumulation and induced cellular apoptosis, but did not affect granulosa cell proliferation. Exposure to BPA increased 17β-estradiol accumulation, promoted granulosa cell proliferation and attenuated T4-induced apoptosis in porcine granulosa cells in vitro.
研究领域:
内分泌学
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生殖生物学
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毒理学
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DOI
PubMed
高影响因子
eLife
·
2023
·
IF 7.7
Zhao-Bo Luo,Shengzhong Han,Xi-Jun Yin,Hongye Liu,Junxia Wang,Meifu Xuan,Chunyun Hao,Danqi Wang,Yize Liu,Shuangyan Chang,Dongxu Li,Kai Gao,Huiling Li,Biaohu Quan,Lin-Hu Quan,Jin-Dan Kang
The host genome may influence the composition of the intestinal microbiota, and the intestinal microbiota has a significant effect on muscle growth and development. In this study, we found that the deletion of the myostatin (MSTN) gene positively regulates the expression of the intestinal tight junction-related genes TJP1 and OCLN through the myosin light-chain kinase/myosin light chain pathway. The intestinal structure of MSTN−/− pigs differed from wild-type, including by the presence of a thicker muscularis and longer plicae. Together, these changes affect the structure of intestinal microbiota. Mice transplanted with the intestinal microbiota of MSTN−/− pigs had myofibers with larger cross-sectional areas and higher fast-twitch glycolytic muscle mass. Microbes responsible for the production of short-chain fatty acids (SCFAs) were enriched in both the MSTN−/− pigs and recipient mice, and SCFAs levels were elevated in the colon contents. We also demonstrated that valeric acid stimulates type IIb myofiber growth by activating the Akt/mTOR pathway via G protein-coupled receptor 43 and ameliorates dexamethasone-induced muscle atrophy. This is the first study to identify the MSTN gene-gut microbiota-SCFA axis and its regulatory role in fast-twitch glycolytic muscle growth.
研究领域:
遗传学
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微生物学
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肌肉生物学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2022
·
IF 3.549
Quancheng Liu,Libo Huang,Zhiwei Cui,Bin Qiao,Fuchang Li,Chunyang Wang
Fumonisin B1 (FB1) has the highest natural contamination rate among all fumonisin analogs and can inhibit food intake and weight gain of pigs. Under laboratory conditions, carboxylesterase FumDSB has a high FB1 degradation rate and excellent pH and thermal stability. The present study sought to estimate the effects of FumDSB on growing pigs from the perspective of a brain–intestinal axis. Twenty-four growing pigs of similar weight were divided into Control, FB1 (5 mg FB1/kg feed), and FumDSB (5 mg FB1/kg and 0.1% FumDSB in the feed) groups. After 42 days of feeding, hypothalamus and jejunum samples were collected for quantitative real-time fluorescence, western blotting, and immunohistochemistry. The results showed that FB1 consumption can destruct the tissue structure of hypothalamus and jejunum, affect the expression and distribution of several appetite-related neuropeptides and inflammatory cytokines, thereby inducing neuroinflammatory responses and affecting food intake and weight gain. However, these anorexia effects and inflammatory responses are alleviated when FumDSB is added to the feed. In short, FumDSB can alleviate the inflammatory response induced by FB1 in growing pigs.
研究领域:
动物科学
·
毒理学
·
营养学
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DOI
PubMed
高影响因子
Frontiers in Immunology
·
2026
·
IF 7
Yiling Zhang,Xianghong He,Qian Zhao,Heping Li,Rui Qin,Weifang Zuo,Bo Han
ObjectiveGrape seed proanthocyanidins (GSP) is a kind of plant polyphenols with a wide variety of biological activities. In the present study, we evaluated whether GSP can alleviate intestinal injury in weaned pigs injected with diquat.MethodsA total of 32 pigs were randomly assigned to four groups (n = 8): (1) nonchallenged control; (2) control + 50 mg/kg GSP; (3) diquat-treated control and (4) diquat + 50 mg/kg GSP. On the morning of day 15, the pigs were injected with diquat (10 mg per kg body weight) or saline, and were then killed to obtain the serum and intestinal segments 7 days later. ResultsThe results showed that GSP addition decreased (P < 0.05) the urea concentration and increased (P < 0.05) the total superoxide dismutase activity in serum of diquat-challenged pigs. GSP increased (P < 0.05) the ileal villus height and duodenal and ileal villus height to crypt depth ratio in diquat-challenged pigs. GSP supplementation not only down-regulated (P < 0.05) the cysteinyl aspartic acid-protease-3 (Caspase-3), Caspase-8 and Caspase-9 expression levels but also up-regulated (P < 0.05) the expression levels of Claudin-1 and zonula occludens-1 in the small intestine. Furthermore, GSP supplementation increased (P < 0.05) the cecal Lactobacillus, Bifidobacterium and Bacillus populations and colonic Bacillus population in diquat-challenged pigs.ConclusionThese findings suggest that GSP can attenuate diquat-induced intestinal mucosa disruption, which was linked to enhancement of the antioxidant capacity and improvement in intestinal microbiota.
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DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 32
·期 16
·2109332
·
IF 18.808
Xianfeng Xia,Xiayi Xu,Bin Wang,Di Zhou,Weili Zhang,Xian Xie,Huasheng Lai,Ju Xue,Aliza Rai,Zhuo Li,Xin Peng,Pengchao Zhao,Liming Bian,Philip Wai-Yan Chiu
Upper gastrointestinal (GI) hemorrhage is a common clinical emergency worldwide. Endoscopic hemostasis is the current first line treatment. Nevertheless, for patients with severe active bleeding and challenging anatomy, endoscopic management can be still difficult and usually requires a higher level of surgical expertise. A simple and effective method of endoscopic hemostasis is therefore in acute clinical demand. Herein a hemostatic hybrid hydrogel comprised of hyaluronic acid and polyethylene glycol stabilized by thiourea–catechol coupling and disulfide bonds is reported. The hybrid hydrogels exhibit enhanced mechanical properties, short gelation time, and good blood clotting ability compared with fibrin gels. The in vivo hemostatic efficacy is confirmed in a rat model of arterial bleeding, in which the rapid gelation of hybrid hydrogels after topical application effectively arrests blood loss with no rebleeding occurring during resuscitation when blood pressure approximately recovers to normal. A large animal study further demonstrates the efficacy of hybrid hydrogels to achieve endoscopic hemostasis against upper GI hemorrhage in pigs. Follow-up observations show that the hybrid hydrogels can remain adherent at the bleeding wound for 48 h, indicating the sustained hemostatic function in vivo. Collectively, this work demonstrates a promising hemostatic hydrogel for endoscopic hemostasis in upper GI hemorrhage.
研究领域:
生物材料
·
医学工程
·
临床医学
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DOI
高影响因子
ACTA OTO-LARYNGOLOGICA
·
2024
·
IF 1.2
Nishan Chen,Tongxiang Diao,Jilei Zhang,Lisheng Yu
Backgrounds Due to the therapeutic needs of inner ear diseases, a simple method of inner ear drug delivery has been under exploration. Postauricular administration, which involves injecting the drug through the middle of the retroauricular groove, is a potential novel approach, but its mechanisms remain unclear.Objectives To investigate threshold shifts in the auditory brainstem response (ABR), as well as the morphology and quantity of hair cells on the bilateral inner ear of guinea pigs after postauricular neomycin administration.Material and Methods Thirty healthy guinea pigs were divided randomly into two groups and administered either neomycin 200 mg/kg or saline 1 ml via daily postauricular injection for 10 days. Auditory threshold-shifts were examined by tests of the ABR. The quantity and morphology of hair cells in the organ of Corti were assessed by confocal laser scanning microscopy.Results The ABR results showed that at a frequency of 16 kHz, the threshold shift was 21.00 ± 14.49 dB in the neomycin injection side, 10.00 ± 16.67 dB in the contralateral side, and 0.50 ± 3.69 dB in the saline group. At a frequency of 24 kHz, the threshold shift was 37.00 ± 16.36 dB in the neomycin injection side, 14.50 ± 9.26 dB in the contralateral side, and 1.00 ± 2.11 dB in the saline group. Histopathological analysis revealed that the outer hair cells (OHCs) in the neomycin injection side suffered significant morphological and numerical loss, while the OHC loss in the contralateral ear was less severe than in the injection side but greater than in the saline group.Conclusions and Significance This study confirms that postauricular administration is a novel method of local drug delivery to the inner ear, which has practical value in future experimental modeling and the treatment of inner ear diseases.
研究领域:
耳科学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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