CHEMISTRY & BIODIVERSITY
·
2024
·Vol. 21
·期 8
·e202400596
·
IF 2.3
Xiao-liang Zhao,Zheng-lei Guo,Kai-li Qi,Qing-song Zhou,Yan-mei Liu,Li-xia Xiong,Jing Zhang,Ji Zhang,Wei-jie Zhang
Three polysaccharides, PTC, PTH, and PTB, were extracted from Pinellia ternata using three different extraction conditions: room temperature water, hot water, and 2 % Na 2 CO 3 solution. PTC and PTH were composed of rhamnose, glucose, galactose, mannose, glucuronic acid, galacturonic acid, and arabinose, which combine to form complex structures. PTB was composed solely of glucose and rhamnose. Further analysis indicated that PTC and PTB exhibited triple-helix structures. PTC showed the highest scavenging capacity against DPPH, superoxide anion, and hydroxyl radicals, with half maximal inhibitory concentrations (IC 50 ) of 1004.1, 1584.1, and 1584.1 μg/mL, respectively. Additionally, PTC, PTH, and PTB were subjected to sulfation, phosphorylation, and selenization, resulting in the production of nine derivates. The distinctive absorptive bands of these derivates were determined through infrared spectroscopy. Selenized and sulfated derivates have shown significant antitumor and immunoenhancing properties. Our findings revealed that at 400 μg/mL, the inhibition rate of selenated PTB on HeLa cells was 54.2 % and that on HepG2 cells was 43.1 %. Additionally, selenized PTC displayed significant immunoenhancing activity, with a proliferation rate of 63.7 % at 400 μg/mL in RAW264.7 cells. These results provide valuable evidence supporting the consideration of polysaccharides from Pinellia ternata as a potential candidate for the development of antineoplastic drugs. Graphical
研究领域:
植物化学
·
药理学
·
天然产物研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2020
·
IF 4.872
Guangjing Ma,Meide Zhang,Jilei Xu,Wuxian Zhou,Liwen Cao
Heat stress has been a major environmental factor limiting the growth and development of Pinellia ternata which is an important Chinese traditional medicine. It has been reported that spermidine (SPD) and melatonin (MLT) play pivotal roles in modulating heat stress response (HSR). However, the roles of SPD and MLT in HSR of P. ternata , and the potential mechanism is still unknown. Here, exogenous SPD and MLT treatments alleviated heat-induced damages in P. ternata , which was supported by the increased chlorophyll content, OJIP curve, and relative water content, and the decreased malondialdehyde and electrolyte leakage. Then, RNA sequencing between CK (control) and Heat (1 h of heat treatment) was conducted to analyze how genes were in response to short-term heat stress in P. ternata . A total of 14,243 (7870 up- and 6373 down-regulated) unigenes were differentially expressed after 1 h of heat treatment. Bioinformatics analysis revealed heat-responsive genes mainly included heat shock proteins ( HSPs ), ribosomal proteins, ROS-scavenging enzymes, genes involved in calcium signaling, hormone signaling transduction, photosynthesis, pathogen resistance, and transcription factors such as heat stress transcription factors ( HSFs ), NACs , WRKYs , and bZIPs . Among them, PtABI5 , PtNAC042 , PtZIP17 , PtSOD1 , PtHSF30 , PtHSFB2b , PtERF095 , PtWRKY75 , PtGST1 , PtHSP23.2 , PtHSP70 , and PtLHC1 were significantly regulated by SPD or MLT treatment with same or different trends under heat stress condition, indicating that exogenous application of MLT and SPD might enhance heat tolerance in P. ternata through regulating these genes but may with different regulatory patterns. These findings contributed to the identification of potential genes involved in short-term HSR and the improved thermotolerance by MLT and SPD in P. ternata , which provided important clues for improving thermotolerance of P. ternata .
研究领域:
植物学
·
分子生物学
·
药用植物学
✓ 已验证
DOI
高影响因子
Journal of Biobased Materials and Bioenergy
·
2017
·Vol. 11
·期 4
·282-290
·
IF 0.975
Zhang Panpan,Yuan Bo,Xu Mengke,Cao Xiaoying,Xue Lingwei,Jiang Jihong
This study aims to investigate the plant growth promoting bacteria (PGPB) in the medicinal plant Pinellia ternata (P. ternata), and evaluate the effect of PGPB on the growth and alkaloid content of P. ternata. Bacteria were isolated from P. ternate, and 16S
rRNA gene sequencing of partial isolates was carried out. 23 kinds of representative bacteria were classified into 7 genera: Acinetobacter, Bacillus, Enterobacter, Pseudomonas, Kosakonia, Rhizobium and Stenotrophomonas. The results of the plant growth promotion screening showed that
23 strains could produce different levels of Indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylicacid deaminase (ACC), biofilm and certain enzymes. Potential PGPB were selected to evaluate their abilities to promote the growth of wheat. The results indicated that strain PtB01 (Rhizobium
nepotum) and PtB24 (Bacillus methylotrophicus) significantly promoted the growth of the wheat seedlings. These two strains and their co-culture group were inoculated on P. ternata aseptic seedlings. It was found that these strains increased shoot length by 194%, root length
by 307%, leaf area by 232%, fresh weight by 231%, dry weight by 165%, and the number of fibrous roots by 200%. We also designed a novel organic silica hybrid monolithic column through silicon dioxide modification and tested the influence of two strains on the alkaloid in P. ternata
aseptic seedlings. The result showed that the content of alkaloid was increased by 140% after inoculation PGPB in P. ternata. PGPB from the medicinal plant P. ternata promoted the growth of the host plant and influenced the alkaloid content of P. ternata.
研究领域:
微生物学
·
生药学
·
分析化学
·
植物科学
✓ 已验证
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Yukun Lin,Kunjing Liu,Jiahui Chen,Tingjie Li,Zijin Sun,Yifan Fan,Peng Zou,Yanting Liu,Chunyu Wang,Chongyang Ma,Xueqian Wang,Fang Lu,Fafeng Cheng,Changming Zhai
A B S T R A C T Ethnopharmacological relevance Pinellia ternata (Ban-xia) and Coptidis Rhizoma (Huang-lian) form a classic herb pair (BXHL) in traditional Chinese medicine that has demonstrated clinical efficacy against Helicobacter pylori ( H. pylori ) infection. However, its pharmacological mechanisms remain insufficiently defined. Aim of the study This study aimed to (1) evaluate the anti- H. pylori colonization effects of BXHL in vitro and in vivo; (2) elucidate its mechanism through the NLRP3-mediated pyroptosis pathway; and (3) assess its influence on gastrointestinal microbiota and identify potential active components. Materials and methods BXHL composition was characterized by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). Anti- H. pylori activity was examined using GES-1 cell co-culture and infected mouse models. Gene expression and signaling pathways were analyzed by RNA-seq, ELISA, RT-qPCR, Western blotting and IHC. Histopathology, bacterial load assays, and ultrastructural observation assessed anti-colonization efficacy. Microbiota alterations were analyzed via 16S rRNA sequencing and molecular docking evaluated interactions between BXHL compounds and NLRP3 targets. Results BXHL significantly reduced H. pylori adhesion, colonization and restored NLRP3-mediated pyroptosis suppressed by infection. Transcriptomic and molecular analyses confirmed reactivation of the NLRP3 signaling cascade. BXHL preserved gut microbial diversity, unlike triple therapy, and exhibited moderate direct antibacterial effects. Conclusion BXHL inhibits H. pylori colonization through suppressing bacterial adhesion factors and activating NLRP3-mediated pyroptosis, while causing no disruption to the gut microbiota. These findings position BXHL as a safe and effective adjunctive therapy, which provides a novel strategy to improve H. pylori eradication that complements conventional antibiotics.
研究领域:
民族药理学
·
微生物学
·
中医中药
·
分子生物学
·
胃肠病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Nanoelectronics and Optoelectronics
·
2022
·Vol. 17
·期 1
·22-29
·
IF 0.697
Meng Lifen,Lv Jingsong,Li Qianzhu,Tan Ye,Xia Zhi,Wu Haizhi
In this study, trace amounts of heavy metal ions copper, lead, mercury and cobalt in Pinellia ternata samples were detected by ionic liquid ultrasonic assisted dispersion liquid–liquid microextraction, combined with high performance liquid chromatography-ultraviolet detector.
The method used sodium diethyldithiocarbamate as complexing agent and phosphoric acid ionic liquid Trihexyl(tetradecyl) phosphine bis[(2,4,4-trimethyl)] amyl phosphonate (cyclophosphate IL104) as extractant. Methanol: 0.2% phosphoric acid (60:40) was used as mobile phase for separation and
UV detector was used for detection, the flow rate was 1 mL/min and the injection volume was 10 μL. In this paper, the effects of sample pH, complexing agent concentration, ultrasonic time, centrifugation time and speed and ionic strength were optimized. Under the optimal conditions
(50 μL ionic liquid cyclic phosphine IL104, pH = 5, 0.5% sodium diethyldithiocarbamate, ultrasonic time of 30 min, centrifugation speed of 4000 r/min, 0.1 g NaCl), the recovery of spiked standard was 89.0~98.2%. High performance liquid chromatography had the characteristics of low
detection limit, accurate and reliable determination results, high sensitivity, strong specificity and good reproducibility.
研究领域:
中药分析
·
环境毒理学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 11
·6149
·
IF 4.9
Lan Mou,Lang Zhang,Yujie Qiu,Mingchen Liu,Lijuan Wu,Xu Mo,Ji Chen,Fan Liu,Rui Li,Chen Liu,Mengliang Tian
Pinellia ternatais a medicinal plant that has important pharmacological value, and the bulbils serve as the primary reproductive organ; however, the mechanisms underlying bulbil initiation remain unclear. Here, we characterized bulbil development via histological, transcriptomic, and targeted metabolomic analyses to unearth the intricate relationship between hormones, genes, and bulbil development. The results show that the bulbils initiate growth from the leaf axillary meristem (AM). In this stage, jasmonic acid (JA), abscisic acid (ABA), isopentenyl adenosine (IPA), and salicylic acid (SA) were highly enriched, while indole-3-acetic acid (IAA), zeatin, methyl jasmonate (MeJA), and 5-dexoxystrigol (5-DS) were notably decreased. Through OPLS-DA analysis, SA has emerged as the most crucial factor in initiating and positively regulating bulbil formation. Furthermore, a strong association between IPA and SA was observed during bulbil initiation. The transcriptional changes inIPT(Isopentenyltransferase),CRE1(Cytokinin Response 1),A-ARR(Type-A Arabidopsis Response Regulator),B-ARR(Type-B Arabidopsis Response Regulator),AUX1(Auxin Resistant 1),ARF(Auxin Response Factor),AUX/IAA(Auxin/Indole-3-acetic acid),GH3(Gretchen Hagen 3),SAUR(Small Auxin Up RNA),GA2ox(Gibberellin 2-oxidase),GA20ox (Gibberellin 20-oxidase),AOS(Allene oxide synthase),AOC(Allene oxide cyclase),OPR(Oxophytodienoate Reductase),JMT(JA carboxy l Methyltransferase),COI1(Coronatine Insensitive 1),JAZ(Jasmonate ZIM-domain),MYC2(Myelocytomatosis 2),D27(DWARF27),SMAX(Suppressor of MAX2),PAL(Phenylalanine Ammonia-Lyase),ICS(Isochorismate Synthase),NPR1(Non-expressor of Pathogenesis-related Genes1),TGA(TGACG Sequence-specific Binding),PR-1(Pathogenesis-related),MCSU(Molybdenium Cofactor Sulfurase),PP2C(Protein Phosphatase 2C), and SnRK(Sucrose Non-fermenting-related Protein Kinase 2) were highly correlated with hormone concentrations, indicating that bulbil initiation is coordinately controlled by multiple phytohormones. Notably, eight TFs (transcription factors) that regulate AM initiation have been identified as pivotal regulators of bulbil formation. Among these,WUS(WUSCHEL),CLV(CLAVATA),ATH1(Arabidopsis Thaliana Homeobox Gene 1), andRAX(Regulator of Axillary meristems) have been observed to exhibit elevated expression levels. Conversely,LEAFYdemonstrated contrasting expression patterns. The intricate expression profiles of these TFs are closely associated with the upregulated expression ofKNOX(KNOTTED-like homeobox), suggesting a intricate regulatory network underlying the complex process of bulbil initiation. This study offers a profound understanding of the bulbil initiation process and could potentially aid in refining molecular breeding techniques specific toP. ternata.
研究领域:
植物生物学
·
分子遗传学
·
药理学
·
转录组学
·
激素调控
✓ 已验证
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Zhi Xia,Yu Zhang,Qianzhu Li,Haijun Du,Guofeng Gui,Gaoyu Zhao
The rapid electrochemical determination of ferulic acid (FA) was assiduously scrutinized by cyclic voltammetry (CV) using graphene oxide sheets (GOs) and multi-walled carbon nanotubes (MWCNTs) nanocomposites modified glassy carbon electrode. The synergistic out-come of GOs and MWCNTs resulted in superior electrocatalysis performance. Under optimal conditions, the peak current was proportional to FA concentration in the range of 0.24 μM to 32 μM, and 88 μM to 1.23 mM, with a relative low detection limit of 0.08 μM. Besides, it was successfully applied to detect FA in Pinellia ternate with recovery values in the range of 102.8%-113.2%, which made it a potential alternative tool for the quantitative detection of FA in pharmaceutical analysis and quality evaluation of traditional Chinese medicine.
研究领域:
药学
·
分析化学
·
传统中药研究
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.5
Mingxiao Feng,Zhenjie Chen,Qian Yu,Chunyan Yan,Dawei Zhang
Pinellia ternata (Thunb.) Breit. has been used in China for more than two thousand years to treat cough and vomiting. P. ternata is rich in polysaccharides; however, current research on the bioactivity of polysaccharides from P. ternata is relatively limited. In particular, there are no reports on their application in the treatment of osteoporosis. In this study, two β -glucan-like polysaccharides were isolated from P. ternata . The primary structures of PTTP50–1-3 and PTTP50–1-4 were comprehensively analyzed using high performance liquid chromatography, gas chromatography–mass spectrometry, and nuclear magnetic resonance. The results indicated that they possess a similar backbone structure. Notably, compared with PTTP50–1-3, PTTP50–1-4 exhibits characteristics of a higher degree of branching, a higher β -glucan content, and a lower molecular weight. In vitro experiments demonstrated that both PTTP50–1-3 and PTTP50–1-4 could increase the alkaline phosphatase activity of MC3T3-E1 cells. In addition, PTTP50–1-4 was able to enhance the mineralization of MC3T3-E1 cells by upregulating the expression of Runx2 , Osx , Bmp2 , Ocn , Opn , and Bsp mRNA. These findings suggest that P. ternata polysaccharides with a β -glucan-like structure may possess strong anti-osteoporotic potential, which provides a theoretical foundation for further research and development of P. ternata polysaccharides in the prevention and treatment of osteoporosis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2026
·
IF 3.6
Yangyi Huang,Jinfei Li,Yue Lu,Jie Cao,Xinzhi Wang,Hao Wu,Min Shen,Hongli Yu
A lectin from Pinellia ternata tubers (PTL, 12 kDa) with potent pro-inflammatory activity binds to the plasma membrane and translocates into the cytoplasm. However, its cellular entry route and inflammatory toxicity mechanisms remain unclear. Here, confocal microscopy showed that fluorescein isothiocyanate (FITC)-labeled PTL (50 μg/mL) accumulated on the cell surface and was endocytosed by RAW264.7 cells time-dependently. Pharmacological inhibition revealed that PTL uptake was mediated by the mannose receptor C-type 2 (MRC2) and predominantly via clathrin-mediated endocytosis. After endocytosis, PTL translocated to mitochondria with marked co-localization, inducing mitochondrial damage characterized by membrane potential dissipation, calcium overload, and excessive mitochondrial reactive oxygen species (mtROS) generation. These alterations promoted intracellular ROS accumulation and amplified inflammatory responses. Co-immunoprecipitation with mass spectrometry identified ATP synthase subunit beta (ATP5B) as a mitochondrial interacting protein of PTL. Their interaction was validated by molecular docking, co-localization, and microscale thermophoresis (MST). ATP5B knockdown markedly attenuated PTL (50 μg/mL)-induced ROS production, inflammatory cytokine expression, and mitochondrial injury, indicating ATP5B is a critical mediator of PTL's inflammatory toxicity. Collectively, these findings demonstrate that PTL induces mitochondrial damage and pro-inflammatory responses in RAW264.7 cells through MRC2-mediated, clathrin-dependent endocytosis and subsequent ATP5B interaction. This study establishes a mechanistic link between lectin endocytosis, mitochondrial targeting, and inflammatory toxicity, providing new insight into plant lectin-mediated immunotoxicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Mingxiao Feng,Qianyu Wang,Minzhen Lei,Dawei Zhang,Qian Yu,Chunyan Yan
Neuroinflammation is closely linked to Alzheimer's disease, where the deposition of amyloid-beta activates microglia, triggering multiple processes that lead to neuronal damage and cognitive decline. However, studies have shown that water extract of Pinellia ternata can improve cognitive function and reduce inflammatory response in the brain injury of mice. Herein, a water-soluble polysaccharide, PTTBP-2-2. with a molecular weight of 11.7 kDa, consisting of D-Glc, D-Gal, D-Xyl, and L-Ara, was isolated from P. ternata . Methylation analysis, partial acid hydrolysis, and nuclear magnetic resonance revealed that the of PTTBP-2-2 backbone consisted of →4)- α -D-Glc p -(1→, →4,6)- α -D-Glc p -(1→, and →6)- α -D-Gal p -(1 → residues, with side chains substituted at O -2, O -3, and O -6 positions of →4)- α -D-Glc p -(1→. In vitro experiments demonstrated that PTTBP-2-2 significantly reduced nitrous oxide levels in BV2 microglia cells and suppressed the expression of pro-inflammatory genes, including COX2 , iNOS , TNF-α , IL-1β , and IL-6 . These factors are key mediators of neuroinflammation. Mechanistic investigations revealed that the anti-neuroinflammatory activity of PTTBP-2-2 was mediated through the regulation of NF-κB and Nrf2/HO-1 signaling pathways. Overall, PTTBP-2-2 shows potential as a therapeutic agent for the treatment of neuroinflammation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 18
·10898
·
IF 6.208
Chenchen Guo,Ying Chen,Dengyun Wu,Yu Du,Mengyue Wang,Cunqi Liu,Jianzhou Chu,Xiaoqin Yao
Pinellia ternata(Thunb.) Druce is a traditional medicinal plant containing a variety of alkaloids, which are important active ingredients. Brassinolide (BR) is a plant hormone that regulates plant response to environmental stress and promotes the accumulation of secondary metabolites in plants. However, the regulatory mechanism of BR-induced alkaloid accumulation inP. ternatais not clear. In this study, we investigated the effects of BR and BR biosynthesis inhibitor (propiconazole, Pcz) treatments on alkaloid biosynthesis in the bulbil ofP. ternata. The results showed that total alkaloid content and bulbil yield was enhanced by 90.87% and 29.67% under BR treatment, respectively, compared to the control. We identified 818 (476 up-regulated and 342 down-regulated) and 697 (389 up-regulated and 308 down-regulated) DEGs in the BR-treated and Pcz-treated groups, respectively. Through this annotated data and the Kyoto encyclopedia of genes and genomes (KEGG), the expression patterns of unigenes involved in the ephedrine alkaloid, tropane, piperidine, pyridine alkaloid, indole alkaloid, and isoquinoline alkaloid biosynthesis were observed under BR and Pcz treatments. We identified 11, 8, 2, and 13 unigenes in the ephedrine alkaloid, tropane, piperidine, and pyridine alkaloid, indole alkaloid, and isoquinoline alkaloid biosynthesis, respectively. The expression levels of these unigenes were increased by BR treatment and were decreased by Pcz treatment, compared to the control. The results provided molecular insight into the study of the molecular mechanism of BR-promoted alkaloid biosynthesis.Keywords:alkaloid;brassinolide;ephedrine;secondary metabolism;RNA-seq
研究领域:
植物学
·
药理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2016
·
IF 3.055
Hui-hui Xie,Jian-ya Xu,Tong Xie,Xin Meng,Li-li Lin,Li-li He,Hao Wu,Jin-jun Shan,Shou-chuan Wang
Ethnopharmacological relevance Banxia (BX) is the root of Pinellia ternata (Thunb.) Berit. Its processed products, such as Jiang Banxia (JBX), have been clinically used in traditional Chinese medicine to treat vomiting, coughing , and inflammation. However, data for their safety for pregnant women are contradictory and confusing. Aim of the study To further explore the safety of BX, an ultra-performance liquid chromatography coupled with liquid chromatography–mass spectrometry (LC–MS) and gas chromatography-mass spectrometry (GC–MS) metabolomics approach was used to evaluate the metabolic perturbation in pregnant rats caused by BX and JBX. Materials and methods Placenta and amniotic fluid samples were collected from control Sprague-Dawley pregnant rats and exposed to BX suspension and JBX decoction (1.434 g/kg/day). Samples were analyzed using LC–MS and GC–MS. The acquired MS data of above samples were further subjected to multivariate data analysis, and the significantly altered metabolites were identified. The associated pathways were constructed using MetaboAnalyst 3.0. Results The weight and histopathology of the placenta from each group of rats had no definite difference. However, we found 20 differential endogenous metabolites that changed significantly in the placenta and amniotic fluid samples. The alterations of identified metabolites indicated a perturbation in glycerophospholipid metabolism, amino acid metabolism, and carbohydrate metabolism in pregnant rats exposed to BX and JBX. Conclusion In summary, this work suggested that oral administration of BX and JBX may induce disturbances in the intermediary metabolism in pregnant rats. This work contributes to further understanding the safety of BX and its processed products.
研究领域:
药理学
·
传统医学
·
代谢组学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）