Waste and Biomass Valorization
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2025
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IF 2.8
Zhou Caixue,Dai Shuxia,Yin Li,Zhuo Yu,Zhao Nannan,Li Dehai,Sun Changyan
Purpose In this paper, we use Pinus pumila (Pall.) Pinecone to extract essential oil and improve its utilization. Methods The study used hydrodistillation to extract essential oil from Pinus pumila (Pall.) pinecone. Inhibition analysis of E. coli using extracted essential oils was evaluated to reveal the inhibitory activity by assessing growth curves, conductivity, nucleic acids, proteins, enzyme activities, and protein synthesis. Results The primary constituents, as shown by GC-MS chemical composition study, were α-pinitol, β-pinene and limonene. The results of the bacteriostatic activity indicated that the inhibitory zone (DIZ) had a diameter of 14.93 ± 0.14 mm, the minimum bactericidal concentration (MBC) was 16.0 µL/mL, and the minimum inhibitory concentration (MIC) was 8.0 µL/mL. Sweeping electron microscopy and laser confocal also demonstrated the disruption of cell wall and cellular enzymes, analyzed by CATase and ATPase activities, which showed a decrease in enzyme activity, and SDS-PAGE analysis showed its ability to inhibit protein synthesis, which leads to cellular damage. Conclusion The present study emphasizes the sustainable use of Pinus pumila (Pall.) Pinecone for economic opening of food preservation in addition to inhibition of spoilage bacteria.
研究领域:
植物学
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微生物学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Hua Zhang,Zhao-Yuan Feng,Zhen-Zhou Li,Yi-Sen Wang,Zhe-Xuan Mu,Xiao-Hong Lv,Zhen-Yu Wang,Zi-Luan Fan
In this study, a polysaccharide component was extracted from Pinus pumila using biomimetic-microwave assisted alkali tandem extraction and then purified by DEAE-52 and Sephadex G-200. A new purified polysaccharide (PSP c-a) was determined through in vitro tracking. The structural features were characterized using a HPGP, FTIR, IC and NMR spectroscopy. The analysis showed that there may be →3, 6)-α-Galp (1 → 3, 4)-α-Glcp (1 → 5)-α-Manp (1→ in the backbone of PSP c-a, →3, 4)-α-Glcp (1→ and →3, 4)-α-Glcp (1 → α-Arap (1→ in the branch of PSP c-a. To improve the effects of PSP c-a on osteoblast MC3T3-E1, PSP c-a was biologically and chemically modified by α-glucosidase together with Sr. Finally, a new thermostable complex Sr-PSP c-a-1 was synthesized. The effects of PSP c-a and Sr-PSP c-a-1 on osteoblasts MC3T3-E1 were compared. It was found that both were found to have a positive effect on MC3T3-E1, and Sr-PSP c-a-1 was more effective than PSP c-a. Therefore, Sr-PSP c-a-1 should be a potential candidate for promoting skeletal tissue regeneration.
研究领域:
生物化学
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材料科学
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医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2016
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IF 3.926
Fengli Chen,Qiang Zhang,Huiyan Gu,Lei Yang
In this study, a novel approach involving homogenate-circulating ultrasound in combination with aqueous enzymatic extraction (H-CUAEE) was developed for extraction of kernel oil from Pinus pumila . Following comparison of enzyme types and concentrations, an enzyme mixture consisting of cellulase, pectinase and hemicellulase (1:1:1, w/w/w) at a concentration of 2.5% was selected and applied for effective oil extraction and release. Several variables potentially influencing extraction yields, namely, homogenization time, incubation temperature, incubation time, mark-space ratio of ultrasound irradiation, ultrasound irradiation power, liquid-solid ratio, pH and stirring rate, were optimized by Plackett-Burman design. Among the eight variables, incubation temperature, incubation time and liquid-solid ratio were statistically significant and were further optimized by Box-Behnken design to predict optimum extraction conditions and ascertain operability ranges for maximum extraction yield. Under optimum operating conditions, extraction yields of P. pumila kernel oil were 31.89 ± 1.12% with a Δ5-unsaturated polymethylene interrupted fatty acid content of 20.07% and an unsaturated fatty acid content of 93.47%. Our study results indicate that the proposed H-CUAEE process has enormous potential for efficient and environmentally friendly extraction of edible oils.
研究领域:
食品科学
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化学工程
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生物技术
✓ 已验证
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2021
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IF 5.645
Xiaojin Peng,Xinyu Yang,Huiyan Gu,Lei Yang,Hongfei Gao
A solvent-free microwave-assisted method and the screw extrusion of raw materials are combined and successfully applied to obtain the essential oil of fresh needles of Pinus pumila (Pall.) Regel. Single-factor screening and the Box-Behnken design method were applied to optimize the extraction of essential oils, and the yield of essential oil under the optimal conditions was 12.00 ± 0.60 mL/kg, which was highly consistent with the results predicted by the Box-Behnken design model. The process developed offers advantages in terms of the yield of essential oil and a reduced extraction time (31 min in comparison to 4 h for conventional hydrodistillation). The microstructures, chemical structures and thermal stability of needle samples before and after extraction were also investigated. The main components found in the essential oil extracted from the fresh needles of P . pumila were (+)-3-carene (22.75 %), δ-cadinene (12.12 %), terpinolene (12.11 %) and β-bisabolene (9.12 %). Furthermore, the semi-inhibition concentrations (IC 50 ) of acetylcholinesterase inhibition activity of the essential oil, terpinolene, (−)- trans -caryophyllene and (+)-3-carene were determined to be 0.76 ± 0.04, 0.55 ± 0.03, 0.45 ± 0.02 and 0.64 ± 0.03 mg/mL, respectively. In general, P . pumila needle essential oil showed notable inhibitory ability to acetylcholinesterase, and the components terpinolene, (−)- trans -caryophyllene, and (+)-3-carene play major roles in the process of essential oils inhibiting acetylcholinesterase. Additionally, the acetylcholinesterase inhibition activities of all the tested samples were mostly related to the corresponding concentrations. The acetylcholinesterase inhibition activity of kojic acid was also determined in parallel experiments as a positive control.
研究领域:
植物化学
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药理学
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生物技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）