Optimization of ultrasound-assisted extraction of phenolic compounds from Pistacia lentiscus with the study of their antioxidant and anti-inflammatory potential
In recent years, significant advancements in the field of green chemistry have spurred the search for alternative solvents for the extraction of phenolic compounds. In this perspective, the objective of this study is to investigate the potential of lactic acid as an alternative solvent for extracting phenolic compounds from Pistacia lentiscus L. Utilizing ultrasound-assisted extraction methodology and response surface methodology, the aim is to optimize the extraction process. Additionally, the study encompasses phytochemical screening, evaluation of biological activity, and characterization of bioactive molecules using liquid and gas chromatography coupled with mass spectrometry. Optimization using the BBD revealed a TPC of approximately 130.17 mg Gallic acid equivalent/g dry weight, achieved at 63.5% lactic acid for 29.67 min at 44.44 °C. Furthermore, in vitro antioxidant tests showed that P.lentiscus extract exhibited potent antioxidant activity with high potential of 186.39 and 176.72 mmol trolox equivalent/100 g dry weight for DPPH• and ABTS•+, respectively. Moreover, P.lentiscus extract demonstrated strong anti-inflammatory activity, with IC 50 values of 0.027%, 0.15%, and 0.034% for NO•, COX and MPO inhibition, respectively. Phytochemical characterization also revealed the presence of organic acids and phenolic compounds, identified as novel molecules characterized for the first time in P.lentiscus leaves. These findings underscore the effectiveness of using lactic acid-based alternative extraction solvents for phenolic compounds, particularly in industrial and pharmaceutical applications.
危险品化学品经营许可证（带存储）