INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Siqing Xiong,Pengcheng Tao,Yuanguo Yu,Wenbing Wu,Yongxin Li,Gang Chen,Jinping Si,Huqing Yang
This study aimed to investigate the effects of raw Polygonatum cyrtonema Hua polysaccharides (RPCPs) and “zhi” P. cyrtonema Hua polysaccharides (ZPCPs) on the gluten structure, in vitro digestion, and shelf life of fresh wet noodles (FWN). The results demonstrated that incorporating PCPs improved the cooking and sensory qualities of FWN. Moreover, the shelf life of FWN was extended by 6 days with 1.5 % RPCPs (w/w) compared with the control FWN. Furthermore, incorporating 1.5 % ZPCPs led to a 1.2- and 0.2-fold increase in the disulfide bond and α-helix content, respectively, compared with the control FWN. This resulted in enhanced gluten structure, improved springiness and viscidity, and reduced cooking loss by 14.47 %–52.19 %. The scanning electron microscopy analysis revealed that the starch particles were entrapped by PCPs, leading to higher gelatinization temperature and lower setback value of FWN, thereby reducing the starch digestion ratio to 55.50 %. In summary, the findings suggested that FWN containing PCPs can extend shelf life, improve taste, and slow starch digestion staple.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Junyin Zhang,Hulan Chen,Lan Luo,Zhipeng Zhou,Yingxiang Wang,Tianyu Gao,Lian Yang,Teng Peng,Mingyi Wu
Polygonatum cyrtonema is a known tonic herb in Chinese Materia Medica , extensively consumed in China, but the structure and activity of its polysaccharide components remain to be clarified. Herein, two new polysaccharides (a fructan and a galactan) were purified from the dried and the processed P. cyrtonema rhizome, respectively. Structural analysis suggested that the fructan consisted of a (2 → 6) linked β- d -Fru f residues backbone with an internal α- d -Glc p residue and two (2 → 1) linked β- d -Fru f residues branches, and that the galactan was a (1 → 4)-β- d -galactan branched with a single β- d -galactose at C-6 at about every nine residues in its main chain. The bioactive assay showed that the fructan and the galactan remarkably promoted growth of Bifidobacterium and Lactobacillus strains, indicating that they possess prebiotic activity. These findings may help expand the application of the polysaccharides from the tonic herb P. cyrtonema as functional ingredients in food products .
研究领域:
药用植物学
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食品科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Baolei Fan,Guoliang Wei,Xiaofeng Gan,Tingting Li,Zengyi Qu,Sheng Xu,Chao Liu,Chunqi Qian
In this study, we reported the various contents of Polygonatum cyrtonema by extracting polysaccharides (PCPs) using steaming and shining procedures, thus providing scientific basis for the processing of Polygonatum cyrtonema and content analysis of polysaccharides. We established the characteristic fingerprint to identify and analyze polysaccharide component changes in distillated and processed Polygonatum cyrtonema by PMP precolumn derivatization and HPLC-MS/MS technology. We also classified two kinds of solutions by comparing the composition difference of 10 polysaccharide components in the samples with principal component and cluster analysis. There was a huge difference between them and seven monosaccharides. Processed PCPs mainly consisted of galactose along with mannose, followed by glucose. Meanwhile, we found that the results of cluster analysis and principal component analysis were the same. The polysaccharide components extracted from processed Polygonatum cyrtonema and distillate were mainly galactose and mannose. The results of cluster analysis and principal component analysis were the same. The distilled liquid can be divided into 5 categories, and the processed dry extract of Polygonatum cyrtonema can be divided into 4 categories. The changing trend of polysaccharide components of Polygonatum cyrtonema and distilled liquid was revealed during the multiple cycles of steaming and sun drying. The similarity and difference of polysaccharide components in the extracted liquid and the distilled liquid of each batch were shown.
研究领域:
中药学
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分析化学
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植物化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2024
·
IF 4.9
Shuanglin Deng,Fang Wang,Meng Xiong,Bangxing Han,Hui Yan
The rapid, accurate, and on-site quantitative evaluation of the 3 components (total polysaccharide, total flavonoid, and total saponin) of Polygonatum cyrtonema Hua (PCH) is of great significance to ensure the value of its medicine and food, for this purpose, a handheld near-infrared (NIR) spectrometer was used. The raw NIR spectra were preprocessed by seven methods and their combinations, then three kinds of wavelength selection algorithms, the competitive adaptive reweighted sampling (CARS), random frog (RF) sampling, and Monte Carlo uninformative variable elimination (MCUVE) were applied, and the characteristic wavelengths for total polysaccharide, total saponin, and total flavonoid in PCH were obtained, respectively. Subsequently, the partial least squares (PLS) regression models were developed. The results showed that the combination of first derivative and standard normal variate was the optimal pretreatment method, and CARS had the highest performance of the wavelength selection for total polysaccharide and total flavonoid, while RF was the highest for total saponin. With these selected wavelengths, 3 PLS prediction models were built, and their performances were validated with unknown samples, the results showed that the determination coefficient of the prediction set (R 2 P ) of total polysaccharide, total saponin, and total flavonoid were 0.980, 0.948, and 0.942, respectively, and the corresponding root mean square error of prediction set (RMSEP) were 0.162, 0.142, and 0.052. Furthermore, the residual prediction deviations (RPDs) of the three components were all larger than 3, demonstrating the prediction performance of the models was high. Therefore, the three active functional components in PCH could be rapidly determined by a handheld NIR spectrometer, which would be beneficial for ensuring the quality of PCH.
研究领域:
中药分析
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光谱学
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产品关联
DOI
高影响因子
CURRENT ISSUES IN MOLECULAR BIOLOGY
·
2026
·Vol. 48
·期 5
·494
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IF 4.1
Daoyuan Li,Jie Wang,Naifu Chen,Naidong Chen
To evaluate the immune-enhancing effects of Polygonatum cyrtonema polysaccharides in vivo, an immunodeficiency zebrafish model was established by microinjecting vinorelbine tartrate into the caudal vein. Effects of the polysaccharides (500, 1000 and 2000 μg/mL) on neutrophil counts were assessed in Tg (mpx:GFP) zebrafish. Transcriptome sequencing was employed to investigate the immunomodulatory effects of the polysaccharides. The results revealed a dose-dependent increase in neutrophil counts following treatment with the polysaccharides. Transcriptomic profiling identified 1286 DEGs across the three comparison groups. GO and KEGG enrichment analyses indicated that the polysaccharides could modulate immune-related pathways in the zebrafish model. Two enriched KEGG pathways, including the MAPK signaling and the mTOR signaling pathway, were utilized to analyze immune-related gene expression. To validate RNA-seq data, qRT-PCR was performed on selected DEGs, including il1b , crk , fgf10b , atp6v1aa , and eif4e1c . The results confirmed that the expression patterns of these genes were consistent with the RNA-seq data. Within the tested concentrations (500, 1000 and 2000 μg/mL), the polysaccharides exhibited a dose-dependent immunostimulatory effect, with the highest immunostimulatory response observed at 2000 μg/mL. The molecular level primarily involves the enhancement of neutrophil function through the modulation of multiple immune-related pathways. These findings provide a theoretical basis for the potential application of Polygonatum cyrtonema polysaccharides as a natural immunomodulatory agent.
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DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL SCIENCES
·
2024
·
IF 3.7
Dong Liu,Tingting Hou,Chunye Geng,Lu Song,Xuefeng Hou,Yanjun Chen,Fang Wang,Wei Wang,Bangxing Han,Leilei Gao
Poor stability and difficult uptake of natural polysaccharides have been the main problems in their application. The purpose of this study was to optimize the preparation conditions of Polygonatum cyrtonema Hua polysaccharides liposomes (PCPL) and to investigate the immune enhancement activity of PCPL in vitro and in vivo, with a view to discovering new ways of natural polysaccharide application. The optimal preparation conditions of PCPL were as follows: the adding amount of Tween 80 of 0.5 %, the ultrasound time of 2 min and the ultrasound times of once. Under these conditions, the entrapment efficiency, drug loading rate and particle size of PCPL were 38.033 %±0.050, 2.172 %±0.003 and 146 nm, which indicated that PCPL with small particle size could be prepared by the reverse-phase evaporation method. Furthermore, PCPL promoted proliferation, phagocytosis, and secretion of nitric oxide and related cytokines in RAW264.7 cells. Moreover, PCPL improved spleen and thymus indices, increased the number or proportion of red blood cells, platelets, and lymphocytes in the blood, and ameliorated spleen and thymus atrophy in immunosuppressed mice. This study provides a new idea for applying Polygonatum cyrtonema Hua polysaccharides (PCP) and references for studying other polysaccharides.
研究领域:
药学
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免疫学
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生物技术
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2026
·Vol. 31
·期 19
·3542
·
IF 5.1
Xueqing Geng,Xingchi Zhang,Yun Wang,Ting Yao,Zhuoting Gan
Polygonatum cyrtonemaHua in Jiuhua Mountain (JHPCH) is a nationally recognized geographically indicated medicinal plant known for its bioactive polysaccharides with diverse pharmacological activities. This study isolated a homogeneous polysaccharide JPCP-70-0 from JHPCH via hot water extraction, ethanol precipitation, and chromatographic purification. Structural characterization by GC-MS, FT-IR, and NMR identified it as a neutral fructan (2.88 × 104Da) with Glc/Fru (6.65%/93.35%), a backbone of →1)-β-D-Fruf-(2→, and side chains of →6)-β-D-Fruf-(2→). Using Caenorhabditis elegans as a model, we evaluated JPCP-70-0’s effects on nematode growth. Results showed concentration-dependent regulation: low concentrations promoted growth, locomotion, feeding, and reproduction, while high concentrations induced developmental inhibition and reduced activity. This work provides a theoretical basis for the rational development of JHPCH resources.
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DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 9
·2076
·
IF 4.2
Ruiding Wen,Lu Luo,Runcheng Zhang,Xudong Zhou,Wei Wang,Limin Gong
A neutralPolygonatumcyrtonemapolysaccharide (NPCP) was isolated and purified fromPolygonatumcyrtonemaby various chromatographic techniques, including DEAE-52 and Sephadex-G100 chromatography. The structure of NPCP was characterized by HPLC, HPGPC, GC-MS, FT-IR, NMR, and SEM. Results showed that NPCP is composed of glucose (55.4%) and galactose (44.6%) with a molecular weight of 3.2 kDa, and the sugar chain of NPCP was →1)-α-D-Glc-(4→1)-β-D-Gal-(3→. In vitro bioactivity experiments demonstrated that NPCP significantly enhanced macrophages proliferation and phagocytosis while inhibiting the M1 polarization induced by LPS as well as the M2 polarization induced by IL-4 and IL-13 in macrophages. Additionally, NPCP suppressed the secretion of IL-6 and TNF-α in both M1 and M2 cells but promoted the secretion of IL-10. These results suggest that NPCP could serve as an immunomodulatory agent with potential applications in anti-inflammatory therapy.
研究领域:
药理学
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免疫学
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生物化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Ling Li,Kiran Thakur,Bu-Yan Liao,Jian-Guo Zhang,Zhao-Jun Wei
We examined the antioxidant and antimicrobial attributes of Polygonatum cyrtonema Hua polysaccharides (HBSS, CHSS, DASS, CASS). The UV spectra and FT-IR results showed the purity and acidic nature of polysaccharides with β -configurations. DASS (78.585% hydroxyl radical scavenging, 92.794% ABTS radical scavenging, 75.648% DPPH radical scavenging activity, 0.582 reducing power and 98.721% Fe 2+ chelation at 5 mg/mL) possessed the best antioxidant potential; while, HBSS (21.113% hydroxyl radical scavenging, 14.412% ABTS radical scavenging, 28.005% DPPH radical scavenging activity, 0.285 reducing power and 48.969% Fe 2+ chelation at 5 mg/mL) was observed with the lowest antioxidant action. The bacterial inhibition effect of four PCHPs on Gram-positive organisms ( B. subtilis and S. aureus with the maximum inhibitory diameters of 18.9 and 15.8 mm, respectively) was better than that of Gram-negative bacteria ( E. coli and S. typhimurium with the maximum inhibitory diameters of 10.9 and 8.4 mm, respectively). The bacterial sensitivity was observed in the following order: B. subtilis > S. aureus > E. coli > S. typhimurium . The trend of antibacterial action of PCHPs was in following order: DASS > CHSS > CASS > HBSS. DASS can be considered as natural anti-oxidant as well as spoilage inhibitors to enhance the nutritive and functional values of various foods.
研究领域:
植物化学
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食品科学
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药理学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Yueyao Gui,Bing Zheng,Hairong Huang,Jianhua Xie,Yi Chen,Jialuo Shan,Xingxing Chang,Xiaobo Hu,Qiang Yu
Alcoholic liver disease (ALD) is characterized by inflammation and oxidative stress induced by excessive alcohol consumption. It is necessary to find a way to use natural active substances to alleviate the liver damage caused by alcohol. The object of this study was to investigate the beneficial effects of pure Polygomatum cyrtonema polysaccharide (PCP-80%) on ALD, and to explore its possible mechanism from the aspect of oxidative stress, inflammation and intestinal microbiota. The results demonstrated that PCP-80% effectively reduced serum biochemical index level and improved liver disease induced by acute alcohol exposure. Furthermore, PCP-80% showed promising outcomes in mitigating oxidative stress and liver inflammation associated with ALD. This might relate to the inhibition of NF-κB pathway and the activation of Keap1/HO-1 pathway. Additionally,PCP-80% enhanced the intestinal barrier and elevated the levels of propionic acid in the colon contents. It also partially reversed the alcohol-induced disturbances in the intestinal microbiome. Overall, PCP-80% exhibited significant therapeutic potential in alleviating acute alcoholic liver injury by reducing oxidative stress, inflammation, and relieving disturbances in the intestinal microbiome. These findings presented a novel approach for the treatment of acute alcoholic liver injury, offering prospects for improving disease prognosis.
研究领域:
药理学
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肝病学
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胃肠病学
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免疫学
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微生物学
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产品关联
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Yanyan Zhang,Shengjun Ma,Haixia Li,Peizi Liu,Keyi Chen,Wei Cai
Research on Polygonatum cyrtonema Hua has focused extensively on its main active components, yet antioxidant peptides remained less explored. This study optimized the preparation process of antioxidant peptides from P. cyrtonema via protein hydrolysis using response surface methodology. The peptide mixture was separated into four fractions using ultrafiltration and G-25 gel chromatography, based on antioxidant capacity. Notably, the F2 exhibited significantly higher Fe 3 ⁺ reducing power (17.17%) and ABTS· radical scavenging rate (30.1%) compared to other fractions at the same concentration. Characterization by Nano-LC-Q Exactive Plus quadrupole Orbitrap MS revealed that F2 contained 11,704 peptides, markedly more than the endogenous (2,454) and crude protein peptides (2,334). Bioinformatics and molecular docking identified three peptides—QFLPF, FVPF, and FPNLF—as having the highest antioxidant potential. These peptides increased cell viability in oxidative damage-induced HepG2 cells by 6.39%, 14.51%, and 9.98%, respectively, compared to the model group. This study lays the groundwork for future mechanistic investigations into the antioxidant peptides of P. cyrtonema.
研究领域:
药理学
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生物化学
·
分子生物学
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分析化学
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产品关联
DOI
高影响因子
Food & Function
·
2022
·Vol. 13
·期 24
·12883-12895
·
IF 6.317
Wei Liu,Taili Shao,Lei Tian,Zhengrui Ren,Lan Gao,Zhiyan Tang,Zheng Fang,Pingchuan Yuan,Chunyan Liu,Jikun Li,Guodong Wang,Jun Han
The chemical structure and pharmacological activity of Polygonatum cyrtonema Hua polysaccharides have garnered significant attention in recent years. In this study, a homogeneous polysaccharide, PCP1, was extracted from P. cyrtonema Hua rhizomes and purified. Monosaccharide composition analysis showed that PCP1 is primarily composed of fructose, glucose, and mannose. Chemical structure analysis showed that the main chain of PCP1 is composed mainly of →1)-β-D-Fruf-(2→ and →1,6)-β-D-Fruf-(2→, with small amounts of →6)-α-D-Glcp-(1→, →4)-β-D-Manp-(1→, and β-D-Glcp-(1→. The side chain is β-D-Fruf-(2→ linked at C-6 of →1,6)-β-D-Fruf-(2→. In vivo experiments showed that PCP1 mitigates liver pathological damage, improves abnormal lipid metabolism and oxidative stress, promotes the production of short-chain fatty acids, and balances the composition of the intestinal microbiota in non-alcoholic fatty liver disease (NAFLD) mice. Thus, PCP1 can be used as a natural ingredient in functional foods for the treatment of NAFLD.
研究领域:
药理学
·
生物化学
·
营养学
·
胃肠病学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）