FOOD RESEARCH INTERNATIONAL
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2022
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IF 7.425
Qingqing Yu,Zhiyu Xiong,Tong Shi,Li Yuan,Yulong Bao,Ruichang Gao
In order to elucidate the gelling mechanism of Premna microphylla turcz (PMT) induced by plant ash (PA), PA was fractionated into supernatant (PA-S) and precipitation (PA-P) and added to the PMT suspension, respectively. The effects of different concentrations (1–9%) and fractions (PA, PA-S, PA-P) of PA suspension on the gel properties were studied. Results showed that the electrical conductivity, content of monovalent cations, pH were higher in PA-S than PA-P. Both the PA-S and PA-P fractions induced the gelation of PMT (except for the low concentration at 1% for PA-S), and the PA-P-PMT gels showed much higher gel strength and hardness than PA-S-PMT gels. With increased concentration, the gel strength increased in PA-P-PMT, but decreased in PA-S-PMT. A hypothesis for the gelation of PMT induced by PA was proposed: the divalent cations in PA bind to carboxyl group in pectin and form gels; when higher content of PA is added, a higher pH leads to extensive dissociation of carboxyl groups thereby increases the electrostatic repulsion between pectin chains, which ultimately weakens the gelling forces. This study can provide theoretical support for further optimization of the traditional processing of PMT gels.
研究领域:
食品科学
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植物学
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DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
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2024
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IF 7
Weiqi Fei,Liyuan Rong,Xin Qi,Xianxiang Chen,Yi Luo,Huiliang Wen,Jianhua Xie
The aim of this study was to investigate the effects of Premna microphylla turcz polysaccharide (PMP) on the rheological, gelling, and structural properties of mung bean starch (MBS) and their potential interaction mechanism. Results showed that the addition of PMP significantly improved the pasting properties, rheological properties, water holding capacity, and thermostability of MBS. The texture tests showed a decrease in hardness, gumminess and chewiness, indicating the retrogradation of MBS was inhibited. Scanning electron microscopy (SEM) suggested the MBS-PMP composite gels expressed a denser microstructure with obvious folds and tears. Moreover, the results of X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and interaction force tests revealed the main forces between MBS and PMP were hydrogen bonds and hydrophobic interactions to form composite gels with great gelling properties. These results facilitate the practical application of MBS and PMP, and provide some references for understanding the interaction mechanism between starch and polysaccharide.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Zeng Dong,Ziqing Du,Xingyue Wu,Kefeng Zhai,Zhaojun Wei,Marwan M.A. Rashed
The current study sought to fabricate pectin nano-films from Premna microphylla Turcz (PMTP) leaves using a combination of ZnO-carboxymethyl cellulose. The rheological and physical properties of fabricated nano-ZnO films were studied. Spectroscopy FT-IR, microscopic study (SEM), thermogravimetry (TG), and XRD were applied to characterize the fabricated film. The antibacterial activity of the nanofilm was determined using the antibacterial circle method. The findings showed that the addition of PMTP can reduce the nanofilm color, water solubility/hydrophilicity, air permeability, and ultraviolet light permeability of the nanofilm. Treatment CPN0.5 achieved the optimized Tensile strength (TS) of 4.50 Mpa, significant differences compared to CPN2 (3.99 Mpa) and CPN1 (3.65 Mpa). In addition, treatment CPN1 achieved the lowest WVP value (29.35) compared to the highest value (41.62) achieved by CPN0.5 treatment with no significant differences with CPN3 (29.7) and CPN1 (30.98) treatments. Elongation (E%) at break was the best for each CP10 (74.9) and CPN0.5 (73.03). Moreover, ZnO can enhance the nanofilm activity and the nanofilm water swelling ratio. Furthermore, adding ZnO to the nano-formula improved the antibacterial activity of the fabricated film against Staphylococcus aureus . In sum, nanofilms fabricated of PMTP and ZnO possess promising prospects as antibacterial agents in packaging applications.
研究领域:
材料科学
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生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Bingmin Wu,Yong Zhu,Yao Liu,Yu Wang,Yuhe Dong,Jihang Chen,Tian Zhong
Bioactive polysaccharide composite gels provide strategy for improving the defects of chitosan (CS) gel. The aim of this study was to characterize the structure of Premna microphylla Turcz polysaccharide (PMTP) and investigate the improvement of different PMTP concentrations (4, 6, 8, 10, and 12 wt%) on the PMTP/CS composite gels' properties. PMTP is an acidic heteropolysaccharide with →4)-α-D-GalpA-(1→ and →4)-α-D-Galpa-6-Ome-(1→ as the backbone structure. And PMTP transformed the gel's framework from the lamellar structure of CS gel to 3D porous network constructed by connected nanofibers. This resulted in the increase of surface area from 11.28 to 89.72 m 2 /g and reduction of pore size from 5.76 to 0.52 μm. Moreover, the mechanical properties of PMTP/CS composite gel was significantly improved 17.9 times higher than that of CS gel. Accordingly, the water holding, swelling, rheology and thermal stability of PMTP/CS gel were further improved. Study on gelation mechanism proved that the formation of composite gel's 3D network was mainly dominated by electrostatic interaction. Finally, Caco-2 cell assay in vitro confirmed the excellent cytocompatibility of PMTP/CS gel. In conclusion, this work provides a scientific reference for the design of bioactive polysaccharide composite gel for food engineering field.
研究领域:
食品科学
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生物材料学
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植物化学
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2025
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IF 12.5
Yuanyuan Chen,Qian Zhou,Yue Zhou,Jinlan Huang,Renjie Niu,Jian Ming,Mingfu Wang
Oral delivery of natural antioxidants represents a promising therapeutic strategy for ulcerative colitis (UC), yet their therapeutic efficacy is hindered by instability and poor accumulation at inflamed sites. To address this, we developed Galectin-3 (Gal-3)-targeted nanoparticles (ZDP-NPs) by encapsulating diosmetin within zein complexes modified with a galactose- and rhamnogalacturonan-I (RG-I)-rich pectin (PMTP, Mw: 228.8 kDa, DM: 34.21 %, RG-I content: 43 %) extracted from Premna microphylla Turcz leaves. Surface plasmon resonance (SPR) analysis confirmed a strong binding affinity between PMTP and Gal-3 (KD = 2.83 μM). ZDP-NPs exhibited uniform spherical morphology (~170 nm), high drug loading (87.9 %) and colloidal stability. Under simulated gastrointestinal conditions, ZDP-NPs showed reduced Dio release (45.6 %) compared to PMTP-free nanoparticles (ZD-NPs, 75.6 %). In vitro studies showed that PMTP surface modification significantly enhanced nanoparticle uptake by LPS-induced macrophages via Gal-3-mediated endocytosis. In vivo colitis experiment of mice revealed that ZDP-NPs selectively accumulated in inflamed colons via Gal-3 targeting, prolonged retention, and significantly alleviated colonic damage by reducing oxidative stress, suppressing pro-inflammatory cytokines, and restoring epithelial barrier integrity. Biocompatibility assays confirmed minimal toxicity of ZDP-NPs. This study presented a food-derived pectin-based nanoplatform for precise colitis intervention through Gal-3 targeting, offering a multifaceted strategy to break the inflammation-oxidative stress cycle.
研究领域:
纳米医学
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药理学
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胃肠病学
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生物化学
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炎症性肠病研究
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植物来源药物
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产品关联
DOI
PubMed
高影响因子