BIOFOULING
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2024
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IF 2.6
Li-Jun He,Zheng-Hui Qiu,Shao-Xia Ma,Rong-Chang Zeng,Cun-Guo Lin
This work investigated the effect of Desulfovibrio caledoniensis (D. caledoniensis) and Pseudomonas aeruginosa (P. aeruginosa) on the microbiologically influenced corrosion (MIC) behaviour of 70Cu-30Ni alloy using surface analysis and electrochemical techniques. The results demonstrated that the mixed medium containing D. caledoniensis and P. aeruginosa further accelerated the MIC of 70Cu-30Ni alloy compared to the single species medium. The addition of exogenous pyocyanin (PYO) to the D. caledoniensis medium increased the maximum pit depth on 70Cu-30Ni alloy from 5.40 μm to 6.59 μm, and the corrosion current density (icorr) increased by one order of magnitude. From the perspective of bioenergetics and extracellular electron transfer (EET), the comprehensive MIC mechanism of 70Cu-30Ni alloy induced by D. caledoniensis and P. aeruginosa was proposed.
研究领域:
微生物学
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材料科学
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腐蚀工程
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PubMed
高影响因子
CATALYSIS LETTERS
·
2020
·Vol. 150
·期 7
·1841-1848
·
IF 2.482
Wu Ting-Ting,Zhao Xiang-Jie,Yang Rong-Ling,Bilal Muhammad,Wang Zhao-Yu,Luo Hong-Zhen,Xu Ning-Ning,Nie Ze-Kun
In this study, lyophilized Pseudomonas aeruginosa based whole-cell biocatalyst was used in the highly efficient and regioselective acylation of helicid with fatty acid vinyl esters in organic solvents. P. aeruginosa whole-cell catalyst showed high biomass and biocatalytic performance using 0.1% (w/v) soybean oil and 0.2% (w/v) tryptone as the carbon and nitrogen source, respectively. The biomass (0.72 g/L) cultivated for 36 h under the optimized conditions was 2-folds higher than that obtained by the use of the initial conditions (0.36 g/L). In addition, the optimized medium was found to be suitable for P. aeruginosa cultured in 3.5-L scale fermentation with high biomass (0.71 g/L) obtained at 24 h. Besides, a high substrate conversion efficiency (ranged from 92 to 99%) and excellent regioselectivity (99%) was achieved when P. aeruginosa whole-cell biocatalyst was applied to synthesize an array of 6′-esters of helicid. The reaction rate varies with varying acyl donors because of their differential interactions with the catalytic site of the enzyme from the whole cells of P. aeruginosa . Taken together, P. aeruginosa whole-cell catalyst obtained using optimal culture medium manifest a promising and highly-regioselective alternative to purified enzymes for the regioselective formation of 6′-esters derivatives of helicid in non-aqueous solvents. Graphic Abstract
研究领域:
生物化学
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酶工程
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工业微生物学
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2019
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IF 6.044
Maruthupandy Muthuchamy,Rajivgandhi Govindan,Kadaikunnan Shine,Veeramani Thangasamy,Naiyf S. Alharbi,Muneeswaran Thillaichidambaram,Jamal M. Khaled,Jun-Li Wen,Khalid F. Alanzi
In this study graphene/chitosan nanoparticles (GR/CS NCs) were developed. The homogenous combination of GR and CS was confirmed by FTIR spectroscopy. The combination of CS with GR sheets reduced the XRD intensity of the GR peak in GR/CS NCs, while TEM images revealed the immobile CS coating of GR sheets. Further, the anti-biofilm activity of GR/CS NCs was tested. The tests showed that the formation of biofilm by Pseudomonas aeruginosa and Klebsiella pneumoniae was inhibited at 40□g/mL GR/CS NCs up to 94 and 92 %, respectively. The intracellular and cell surface damage of the bacteria was observed by CLSM and SEM. Also, GR/CS NCs produced a toxic effect of 90 % on Artemia franciscana at 70□g/mL upon 24 h incubation. The recorded properties of the synthesized GR/CS NCs qualify them as potential agents against multi-drug resistant bacteria.
研究领域:
微生物学
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纳米医学
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药理学
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DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2024
·
IF 4
Liu Chang,Cui Chenxuan,Tan Xiaoxin,Miao Junjie,Wang Wei,Ren Han,Wu Hua,Zheng Cuiying,Ren Huan,Kang Weijun
The emergence of multidrug-resistant Pseudomonas aeruginosa isolates is a growing concern for public health, necessitating new therapeutic strategies. Gallium nitrate [Ga(NO3)3], a medication for cancer-related hypercalcemia, has attracted great attention due to its ability to inhibit P. aeruginosa growth and biofilm formation by disrupting iron metabolism. However, the antibacterial efficacy of Ga(NO3)3 is not always satisfactory. It is imperative to investigate the factors that affect the bactericidal effects of Ga(NO3)3 and to identify new ways to enhance its efficacy. This study focused on the impact of pH on P. aeruginosa resistance to Ga(NO3)3, along with the underlying mechanism. The results indicate that acidic conditions could increase the effectiveness of Ga(NO3)3 against P. aeruginosa by promoting the production of pyochelin and gallium uptake. Subsequently, using glutamic acid, a clinically compatible acidic amino acid, the pH was significantly lowered and enhanced the bactericidal and inhibitory efficacy of Ga(NO3)3 against biofilm formation by P. aeruginosa, including a reference strain PA14 and several multidrug-resistant clinical isolates. Furthermore, we used an abscess mouse model to evaluate this combination in vivo; the results show that the combination of glutamic acid and Ga(NO3)3 significantly improved P. aeruginosa clearance. Overall, the present study demonstrates that acidic conditions can increase the sensitivity of P. aeruginosa to Ga(NO3)3. Combining glutamic acid and Ga(NO3)3 is a potential strategy for the treatment of P. aeruginosa infections.
研究领域:
微生物学
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感染与免疫
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药理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2016
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IF 3.994
Y. Long,Z. Li,Q. Bi,C. Deng,Z. Chen,S. Bhattachayya,C. Li
Considering outburst of various infectious diseases globally, nanoparticle assisted targeted drug delivery has emerged as a promising strategy that can enhance the therapeutic efficacy and minimize the undesirable side effects of an antimicrobial agents. Molecular imprinting is a newly developed strategy that can synthesize a drug carrier with highly stable ligand-like ‘cavity’, may serve as a new platform of ligand-free targeted drug delivery systems. In this study, we use the amphiphilic lipopolysaccharides, derived from Pseudomonas aeruginosa as imprinting template and obtained an evenly distributed sub-40 nm polymeric nanoparticles by using inverse emulsion method. These molecularly imprinted nanoparticles (MIPNPs) showed specific binding to the lipopolysaccharide as determined by fluorescence polarization and microscale thermophoresis. MIPNPs showed selective recognition of target bacteria as detected by flow cytometry. Additionally, MIPNPs exhibited the in vivo targeting capabilities in both the keratitis model and meningitis model. Moreover, the photosensitizer methylene blue-loaded MIPNPs presented significantly strong inhibition of bacterial Growth, compared to non-imprinted controls for in vitro model of the photodynamic therapy. Our study shows an attempt to design a magic bullet by molecular imprinting that may provide a novel approach to generate synthetic carrier for targeting pathogen and treatment for a variety of infectious human diseases.
研究领域:
微生物学
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药剂学
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感染病学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2026
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IF 5.4
YaTong Zhang,Xuelin Sun,HuiBo Li,DanDan Li,Caiyuan Yu,Bin Zhao,YingChen Zhou,Yi Zhun Zhu,Rongsheng Zhao
Introduction Antibiotic resistance has become a global health crisis, driving the urgent need for novel antibacterial strategies. Antimicrobial peptides (AMPs) have emerged as promising therapeutic candidates due to their broad-spectrum activity and low propensity for resistance development. However, conventional discovery approaches remain time-consuming and inefficient for large-scale screening. Methods In this study, we developed an integrated prediction framework that combines deep learning with classical machine learning methods to enable rapid and accurate identification of AMPs. The model was applied to screen over 250,000 artificially generated peptide sequences. Candidate peptides were selected based on physicochemical descriptors and activity-associated features, followed by solid-phase synthesis and minimum inhibitory concentration assays against Pseudomonas aeruginosa. Mechanistic investigations were conducted using scanning electron microscopy to visualize membrane damage, molecular dynamics simulations to probe peptide-membrane interactions, and transcriptomic profiling to assess bacterial stress responses and metabolic pathway alterations. Results Ten promising antibacterial candidates were successfully identified and experimentally validated. All selected peptides exhibited measurable activity against P. aeruginosa, with several showing potent inhibitory effects. Microscopic and simulation analyses confirmed that the peptides exert their antibacterial effects primarily through membrane disruption. Transcriptomic data further revealed that these peptides interfere with key metabolic pathways and activate bacterial stress response systems. Discussion Our findings demonstrate that the integrated deep learning-machine learning pipeline offers an efficient and reliable approach for large-scale AMP discovery. The mechanistic insights gained from this study not only validate the predicted candidates but also provide a foundation for rational optimization of peptide-based therapeutics. This comprehensive strategy holds promise for accelerating the development of next-generation alternatives to conventional antibiotics.
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DOI
PubMed
高影响因子
Food Science & Nutrition
·
2021
·Vol. 9
·期 7
·3873-3884
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IF 2.863
Mingcan Qin,Xiaoyi Ma,Shihui Fan,Hangjie Wu,Wanli Yan,Xiaopeng Tian,Jing Lu,Mingsheng Lyu,Shujun Wang
In the present study, a DNAzyme was screened in vitro through the use of a DNA library and crude extracellular mixture (CEM) of Pseudomonas aeruginosa . Following eight rounds of selection, a DNAzyme termed PAE-1 was obtained, which displayed high rates of cleavage with strong specificity. A fluorescent biosensor was designed for the detection of P. aeruginosa in combination with the DNAzyme. A detection limit as low as 1.2 cfu/ml was observed. Using proteases and filtration, it was determined that the target was a protein with a molecular weight of 10 kDa–50 kDa. The DNAzyme was combined with a polystyrene board to construct a simple indicator plate sensor which produced a color that identified the target within 10 min. The results were reliable when tap water and food samples were tested. The present study provides a novel experimental strategy for the development of sensors based on a DNAzyme to rapidly detect P. aeruginosa in the field.
研究领域:
分子生物学
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微生物学
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生物传感技术
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DOI
PubMed
高影响因子
RSC Chemical Biology
·
2023
·Vol. 4
·期 10
·774-784
·
IF 4.1
Xiaojun He,Bingjie Han,Runming Wang,Yu Guo,Richard Y T Kao,Hongyan Li,Hongzhe Sun,Wei Xia
The opportunistic pathogen Pseudomonas aeruginosa (P. aeruginosa) causes infections that are difficult to treat, which is due to the bacterial natural resistance to antibiotics. The bacterium is also able to form a biofilm that protects the bacterium from clearance by the human immune system and leads to chronic infection. Herein, we synthesized and characterized a novel gallium compound that interferes with both the iron metabolism and quorum sensing system of P. aeruginosa to achieve a significant bactericidal activity. The compound could substantially reduce the secretion of bacterial virulence factors as well as eliminate biofilm formation. Integrative omics analysis indicates that this compound can significantly disturb the gene transcription and metabolism of P. aeruginosa. The effectiveness of the gallium compound was further validated in mammalian cell and murine skin infection models. Our study offers a new strategy to design new gallium-based antimicrobials to combat P. aeruginosa infection.
研究领域:
微生物学
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药理学
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感染病学
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DOI
PubMed
高影响因子
WATER RESEARCH
·
2024
·
IF 11.4
Tianyu Gao,Ying Li,Ke Dai,Fangang Meng
In natural or engineered anaerobic environments, iron oxidation-driven microbial denitrification plays a critical role in the water or wastewater treatment. Herein, we report a previously unidentified metallic iron (Fe 0 )-dependent denitrification mode driven by the electro-syntrophic interaction between electroactive microorganism and denitrifier. In a model denitrifying consortium of Shewanella oneidensis and Pseudomonas aeruginosa , we find that P. aeruginosa can accept electrons for nitrate reduction via the constructed electron transfer system of Fe 0 – S. oneidensis – P. aeruginosa . In the electro-syntrophic consortium, the membrane-bound CymA–OmcA–MtrC protein complexes of S. oneidensis drive the generation, transfer and consumption of electrons, thus enabling modulation of microbial metabolic activity. Specially, using Fe 0 as the sole electron donor, S. oneidensis can act as a bio-engine to harvest electrons and conserve energy from Fe 0 biocorrosion. Electrons released by S. oneidensis are utilized by P. aeruginosa for accomplishing microbial denitrification. Metatranscriptomics analysis demonstrated that the direct electron cross-feeding process facilitates the expression of genes encoding for denitrification enzymes, intracellular electron transfer proteins, and quorum sensing of P. aeruginosa . The Fe 0 -dependent electronic syntrophy in this work could provide a metabolic window for the growth of denitrifiers that is a new insight into nitrate removal or global nitrogen cycle.
研究领域:
环境微生物学
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生物地球化学
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废水处理
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微生物生态学
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DOI
PubMed
高影响因子
iScience
·
2025
·
IF 4.1
Jie Liu,Youzhi Li,Kairuo Yan,Jinghua Han,Ziqi Wu,Ying Li
Pseudomonas aeruginosa ( P. aeruginosa ) biofilms pose substantial challenges in clinical settings due to their resistance to conventional antibiotic treatments. This study investigated the influence of reductive stress on the formation and eradication of P. aeruginosa biofilms. A series of redox-active compounds were employed to assess their impact on both biofilm development and disruption. Among them, hydrazine (HZ) showed potent activity. Mechanistic studies revealed that these compounds suppressed pyocyanin production and impaired bacterial metabolic activity. A structure–activity relationship analysis highlighted hydroxylamine (HA) as a promising candidate, owing to its favorable balance between efficacy and cytotoxicity. Notably, quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis of HA-treated P. aeruginosa suggested inhibition of matrix biosynthesis, quorum sensing, and oxidative stress defenses. This study provides insights into biofilm management by targeting the redox environment, offering potential strategies for the development of redox-based therapies to combat biofilm-associated infections.
研究领域:
微生物学
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传染病学
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生物膜研究
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氧化还原生物学
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抗菌治疗
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产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Wang Meng,Deng Zifeng,Li Yanmei,Xu Keyong,Ma Yi,Yang Shang-Tian,Wang Jufang
Pseudomonas aeruginosa (P. aeruginosa), an opportunistic pathogen, is often associated with difficulties in treating hospital-acquired infections. Biofilms formed by P. aeruginosa significantly improve its resistance to antimicrobial agents, thereby, posing a great challenge to the combat of P. aeruginosa infection. Antimicrobial peptides (AMPs) have recently emerged as promising antibiofilm agents and increasingly attracting the attention of scientists worldwide. However, current knowledge of their antibiofilm behavior is limited and their underlying mechanism remains unclear. In this study, a novel AMP, named PEW300, with three-point mutations (E9H, D17K, and T33A) from Cecropin A was used to investigate its antibiofilm property and antibiofilm pathway against P. aeruginosa. PEW300 displayed strong antibacterial and antibiofilm activity against P. aeruginosa with no significant hemolysis or cytotoxicity to mouse erythrocyte and human embryonic kidney 293 cells. Besides, the antibiofilm pathway results showed that PEW300 preferentially dispersed the mature biofilm, leading to the biofilm-encapsulated bacteria exposure and death. Meanwhile, we also found that the extracellular DNA was a critical target of PEW300 against the mature biofilm of P. aeruginosa. In addition, multiple actions of PEW300 including destroying the cell membrane integrity, inducing high levels of intracellular reactive oxygen species, and interacting with genomic DNA were adopted to exert its antibacterial activity. Moreover, PEW300 could dramatically reduce the virulence of P. aeruginosa. Taken together, PEW300 might be served as a promising antibiofilm candidate to combat P. aeruginosa biofilms.
研究领域:
微生物学
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分子生物学
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传染病学
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药学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2020
·
IF 7.1
Yujie Gao,Yu Chen,Miaomiao Li,Lizhen Jia,Lianbin Zhang,Jintao Zhu
Identification or detection of pathogenic microbes is one of the most important aspects to prevent and/or treat bacterial infection. In this study, we report a facile yet versatile strategy for preparation of gelatin-based photonic hydrogel sensors for visual detection of Pseudomonas aeruginosa ( P. aeruginosa ). The photonic hydrogels are generated by in-situ photopolymerization of acrylamide (AM), N , N ’-methylenebis-(acrylamide) (BIS), and gelatin methacrylate (GelMA) with the self-assembled one-dimensional (1D) chain-like structures of carbon-encapsulated Fe 3 O 4 nanoparticles (Fe 3 O 4 @C NPs). In the presence of P. aeruginosa , the hydrolysis of gelatin triggered by gelatinase reduces the crosslinking density of photonic hydrogel, expands the hydrogel in volume, increases the lattice spacing of the colloidal particles, and red-shifts the reflection spectra of the photonic hydrogel. The optical appearance variation of the photonic hydrogel, resulting from the reflection peak shift, can be observed by naked eyes. Besides, we show that the photonic hydrogels exhibit negligible appearance changes with the presence of Escherichia coli ( E. coli ), indicating their potential for distinguishing various bacteria by a simple structural color variation. This finding provides a proof-of-concept for a colorimetric sensor for visual detection of P. aeruginosa and demonstrates the great potential of the photonic hydrogels in visual detection of pathogens.
研究领域:
生物医学工程
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微生物学
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材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）