Probiotics and Antimicrobial Proteins
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2023
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IF 4.9
Shen Yong,Bai Xue,Wang Jiaxi,Zhou Xiran,Meng Rizeng,Guo Na
Methicillin-resistant Staphylococcus aureus (MRSA) is a notorious pathogen with biofilm-forming and drug-resistant properties that make it difficult to eradicate. In this study, the inhibition of MRSA (ATCC 43300) by Starmerella bacillaris CC-PT4 (CGMCC No. 23573) was evaluated. The results showed that the inhibition of MRSA growth and biofilm was caused by S. bacillaris CC-PT4 cell-free supernatant (CFS). The CFS of S. bacillaris CC PT4 at different times can effectively inhibit the formation of MRSA biofilm, remove the preformed biofilm, and down-regulate the related genes that promote the formation of biofilm. Afterwards, untargeted metabolomics was performed to analyze the CFS of S. bacillaris CC-PT4. Several molecules with antibacterial and inhibitory biofilm effects from the CFS were found, one of which, 2-amino-1-phenylethanol (APE), has not been reported to have antiMRSA ability before. In this study, molecular docking analysis and in vitro experiments were used to verify the function of APE to inhibit MRSA. These results indicate that S. bacillaris CC-PT4 CFS can effectively inhibit MRSA which has potential application value in controlling MRSA.
研究领域:
微生物学
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医学
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药理学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Mingyue Chang,Moru Zhang,Haihong Hu,Shucai Liang
A novel polyethyleneimine (PEI)-based polymeric nanosensor (named PEIMP) was developed for specific fluorescence enhanced sensing of Pt 4+ ion in aqueous media. The sensor was fabricated via “one-pot” three-component reaction using ortho -phthalaldehyde (OPA), PEI and mercaptopurine as raw materials, by which the formation of isoindole fluorophore and its chemical grafting onto PEI chain were achieved simultaneously. The morphology, size and structure of PEIMP have been characterized by various techniques. In buffered aqueous solution (pH 7.0), PEIMP had the ability to specifically bind with Pt 4+ producing notable increase in fluorescence emission at 463 nm (excited at 395 nm). Based on investigations on the sensing mechanism, the fluorescence turn-on response towards Pt 4+ was attributed to the binding of Pt 4+ with purine group in PEIMP resulting in the inhibition of photoinduced electron transfer from purine to isoindole fluorophore. Under the optimal conditions (pH 7.0, incubated at 37 ℃ for 20 min) the detection of Pt 4+ could be achieved with the linear range of 0.1–10 μM and the detection limit of 80 nM. The sensor had the advantages of low-cost raw materials, simple and environmental-friendly synthesis and analytical detection procedures. What's more, it could selectively and sensitively detect Pt 4+ without the effects from common transition metal ions (Pb 2+ , Fe 3+ , Cr 3+ , Al 3+ , Ag + , Co 2+ , Hg 2+ , Cd 2+ , Cu 2+ , Mg 2+ , Ni 2+ , Mn 2+ , Zn 2+ ), especially precious metal ions of Pt 2+ and Pd 2+ . The proposed method had been successfully applied to quantify Pt 4+ in wastewater and urine samples, and also proved to be potential for monitoring Pt 4+ in biological systems.
研究领域:
分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Nano Materials Science
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2026
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IF 20.5
Zhiyu Pei,Szymon Sobczak,Shuailing Ma,Tian Cui,Laihui Luo,Peng Du
Pt 4+ -doped Cs 2 ZrCl 6 double perovskites were synthesized through a rapid room-temperature synthetic approach. Excited by 365 nm, the obtained compounds exhibit intense red photoluminescence, originating from self-trapped exciton emission. In-situ high-pressure absorption spectroscopy was employed to analyze the pressure-driven evolution of the optical band gap. Moreover, high-pressure X-ray diffraction and Raman spectroscopy experiments indicate a pressure-induced phase transition at around 24 GPa. With compression from 0.27 GPa to 41.56 GPa, the synthesized samples not only exhibit a distinct spectral blue shift but also show enhanced luminescence intensity, leading to the multicolor emission and high-pressure zero-quenching luminescence behavior. Furthermore, the occurrence of the pressure-induced phase transition is visible from the changes in the full width at half maximum (FWHM) of the emission band, which first decreases and then increases with compression, reaching a minimum value at 24.32 GPa. Such strong changes of the emission band centroid and FWHM were employed as the manometric parameters, with maximum absolute sensitivities of 5.35 nm GPa −1 and 2.19 nm GPa −1 , respectively. Additionally, the developed compounds exhibit colorimetric pressure sensing with a maximum relative sensitivity of 2.32% GPa −1 . These findings highlight that the Pt 4+ -doped Cs 2 ZrCl 6 double perovskites hold great potential for multi-parameter, wide-operating range, and high-sensitivity visual pressure sensors.
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DOI
高影响因子
ACS Biomaterials Science & Engineering
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2024
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IF 5.4
Chang Liu,Zhengjiang Cao,Li Li,Qingyin Li,Chunle Zhang,Yunbing Wang,Linhua Li,Ping Fu
Sepsis is a severe and complex systemic infection that can result in multiple organ dysfunction. Sepsis-associated acute kidney injury (SAKI), caused by inflammatory response, oxidative stress, and cellular apoptosis, is a common complication that seriously impacts patient survival rates. Herein, a potent and novel metal-polyphenol nanomicelle can be efficiently self-assembled with Pt4+ and honokiol (HK) by the chelation, π–π conjugation, hydrophobic action, and the surfactant properties of Tween-80. These nanomicelles not only enhance drug bioavailability (encapsulation rates: Pt─49%, HK─70%) and reduce drug toxicity (safety dose: <20 μg/g) but also improve targeting toward damaged renal tissues. Furthermore, Pt4+ and HK in the nanomicelles exert a synergistic physiological effect by scavenging free radicals to alleviate oxidative damage, inhibiting macrophage activation and the release of inflammatory factors to regulate inflammation, and displaying broad-spectrum antimicrobial activity to control infection. These actions collectively protect renal tissue and restore its functionality. Here, we constructed metal-polyphenol nanomicelles (Pt/HK-NMs) via ingenious and efficient self-assembly, providing a new strategy to compensate for deficiencies in the hemodialysis and antibiotic treatment of SAKI.
研究领域:
药学
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纳米技术
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感染病学
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肾脏病学
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产品关联
DOI
PubMed
高影响因子
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