Renjie Li,Junyuan Li,Jiangtao Shi,Yongyue Zhang,Yuxin Sun,Yuxi Chen,Zhipeng Liu
The water immersion ofPterocarpuswood produces strong blue fluorescence, which comes from the extract. The fluorescence contained in the extract is of interest for the identification ofPterocarpuswood. We conducted an investigation into the extraction solution ofPterocarpuswood and analyzed the mechanism of fluorescence in this species. Possible species of the fluorescent molecules are discussed based on the mixture. Liquid chromatography mass spectrometry (LC-MS) is used for an analysis of the extract, the obtained substances that may be fluorescent inPterocarpuswood. In addition, the change in the fluorescence intensity with changes in the pH and concentration in the extract is also studied. The results show that the fluorescent molecule is quenched by aggregation (Aggregation-Caused Quenching; ACQ) and is unstable in over-acidic and over-alkaline conditions (especially acidic).Keywords:Pterocarpuswood;extract solution;fluorescence intensity;conjugate structure
Renjie Li,Haizhe Zhang,Weiqi Leng,Zhipeng Liu,Jiangtao Shi
At present, excessive Fe 3+ in daily water has become a threat to human health. Among the conventional detection methods for Fe 3+ , fluorescent probes have been applied on a large scale due to their simplicity and efficiency. However, the currently available fluorescent probes are difficult to synthesize, costly and environmentally unfriendly, limiting their applications. In this work, a fluorescent extract of Pterocarpus wood was successfully obtained, and the structure of some coumarin-based molecules in this extract was determined by 2D-NMR. Subsequently, the intensity of this fluorescence was optimized using response surface methodology (RSM), resulting in a high-intensity fluorescent probe. The probe was sensitive to the concentrations of Fe 3+ and MnO 4 − , and could efficiently detects Fe 3+ in the range of 2.7 μM–8.0 μM, with LOD and LOQ reaching 1.06 μM and 3.20 μM, respectively. Moreover, based on the strong complexation property of EDTA on Fe 3+ , this work designed the "switch-on" fluorescent probes . The experiment shows that both static and dynamic quenching exist in this system. The mechanism of complexation and oxidation of fluorescent molecules by the quencher is interpreted in the quenching reaction. In addition, the fluorescent probe has a high yield and low cost, it also performs well in actual water sample tests. This method is expected to be developed as a new way on Fe 3+ detection.
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