JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Wanxian Wang,Yanfang Liu,Dian Liu,Han Zhou,Yan Li,Wenjie Yuan,Suowen Xu,Jixia Wang,Xinmiao Liang,Jianping Weng
Pueraria lobata (Willd.) Ohwi, known as kudzu and used as a “longevity powder” in China, is an edible plant which is rich in flavonoids and believed to be useful for regulating blood sugar and treating diabetes, although the modes of action are unknown. Here, a total of 53 flavonoids including 6 novel compounds were isolated from kudzu using multidimensional preparative liquid chromatography. The flavonoid components were found to lower blood sugar levels, promote urine sugar levels in mice, and reduce the urine volume. Molecular docking and in vitro assays suggested that the antidiabetic effect of kudzu was attributed to at least three targets: sodium-dependent glucose transporter 2 (SGLT2), protein tyrosine phosphatase-1B (PTP1B), and alpha-glucosidase (AG). This study suggests a possible mechanism for the antidiabetic effect that may involve the synergistic action of multiple active compounds from kudzu.
研究领域:
药理学
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生物化学
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植物科学
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产品关联
DOI
PubMed
高影响因子
Materials Today Communications
·
2024
·
IF 3.7
Doudou Ma,Chuanhui Zhang,Yilong Wei,Li Ma,Xiaoliang Zhao,Weijie Zhang
Type 2 diabetes (T2D) is a chronic metabolic disorder characterised by hyperglycaemia, insulin resistance, and relative insulin deficiency. Insulin resistance results in patients requiring increased insulin injections and its limited bioavailability. The present subject matter aims to use Pueraria lobata polysaccharides, which can alleviate insulin resistance and have hypoglycaemic potency, as a medicinal excipient to prepare a hydrogel with excellent slow-release properties, which can be used to load insulin for injection or as a diabetic wound dressing material. Pueraria lobata polysaccharides (PLR) were extracted by hydroalcohol precipitation, and oxidised Pueraria lobata polysaccharides (O-PLR) were successfully prepared by sodium periodate oxidation and verified by structural characterisation. Subsequently, double crosslinked hydrogels were prepared using oxidised Pueraria lobata polysaccharides, chitosan and cellulose acetoacetate, and several of their properties were investigated in detail. The experimental results showed that the O-PLR double-crosslinked hydrogels possessed good rheological properties and injectability, as well as excellent self-healing and antimicrobial activities; moreover, the O-PLR hydrogels exhibited significant hypoglycemic activity, which is expected to be an important material for the treatment of Type 2 diabetes mellitus. Overall, this study provides a reliable theoretical and experimental basis for the development of hydrogels with hypoglycaemic, pro-healing and antibacterial functions.
研究领域:
药学
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生物材料学
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糖尿病研究
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2023
·Vol. 24
·期 7
·6247
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IF 5.6
Shifan Zhao,Hualei Xue,Yijiong Tao,Kai Chen,Xiao Li,Mi Wang
A novel water-soluble acidic heteropolysaccharide, called PPL-1, was purified fromPueraria lobata. PPL-1 had an average molecular weight of 35 Kad, and it was composed of glucose, arabinose, galactose and galacturonic acid (6.3:0.8:0.8:2.1). In accordance with methylation and nuclear magnetic resonance analyses, PPL-1 primarily consisted of (1→2)-linked α-Araf, (1→4)-linked α-Glcp, (1→)-linked β-Glcp, (1→6)-linked α-Glcp, (1→3,6)-linked α-Galp, (1→)-linked β-GalpA and (1→4)-linked α-GalpA. In terms of bioactivities, PPL-1 exhibited remarkable scavenging ability towards DPPH (1,1-Diphenyl-2-picrylhydrazyl) radicals and moderate activity by enhancing the proliferation rate of RAW 264.7 cells by approximately 30% along with the secretion of NO. This work demonstrates that PPL-1 can be a potential source of immunoenhancers and antioxidants.Keywords:Pueraria lobata;polysaccharide;structure;bioactivity
研究领域:
生物化学
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药理学
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免疫学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
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IF 5.162
Rong Chen,Bo Liu,Xiaoyang Wang,Kai Chen,Keyu Zhang,Lifang Zhang,Chenzhong Fei,Chunmei Wang,Yingchun Liu,Feiqun Xue,Feng Gu,Mi Wang
Polysaccharide was derived from Pueraria lobata (PPL) which was considered as one of the traditional Chinese medicinal and edible herbs. In the present study, PPL was administered in equal doses (12.5 mg/kg) to both normal mice and antibiotic-associated diarrhea (AAD) mice for two weeks, and was evaluated in terms of body weight, organ indices, gut structure, gut microbiota and short chain fatty acids. The results showed that normal mice treated with PPL not only reduced the isovaleric acid concentration ( P < 0.05), but also significantly increased the abundance of beneficial bacteria, involving Oscillospira and Anaerotruncus ( P < 0.05). In addition, PPL could relieve colonic pathological changes and gut microbiota dysbiosis caused by AAD. It indicated that PPL was a potential functional food ingredient by modulating gut microbiota.
研究领域:
药理学
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微生物学
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营养学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Wei Ding,Rui Wu,Jie Guo,Xianwu Zhou,Shima Liu,Jian He,Ke Song
Resveratrol is a naturally occurring polyphenol with pronounced bioactivity. Its cardioprotective, anti-inflammatory, and antioxidant properties confer broad potential for disease prevention and therapy. However, its poor aqueous solubility and limited stability constrain practical applications. In this study, Pickering emulsions stabilized by octenyl succinic anhydride (OSA)-modified starch nanoparticles (OSA-SNPs) were developed to enhance the physicochemical stability and bioavailability of resveratrol. SNPs were prepared from Pueraria lobata starch by antisolvent precipitation using ethanol and were subsequently modified with OSA to yield OSA-SNPs. Starch-based Pickering emulsions stabilized with OSA-SNPs were formulated for resveratrol encapsulation. The encapsulation efficiency, droplet size, and emulsion stability were characterized. Stable emulsions with an encapsulation efficiency of 92.16 % were obtained. Compared with surfactant-stabilized systems, the starch-based Pickering emulsions exhibited superior resistance to Ostwald ripening and improved biocompatibility. In vitro simulated gastrointestinal digestion studies demonstrated a sustained-release profile of resveratrol over time. These findings indicate that OSA-modified Pueraria lobata starch nanoparticles are a promising green stabilizer for delivery systems of poorly soluble and labile bioactive compounds, offering a practical strategy for their application.
研究领域:
食品科学与技术
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纳米技术
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药物递送
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胶体化学
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功能食品
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产品关联
DOI
PubMed
高影响因子
Food & Function
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2020
·Vol. 11
·期 8
·7104-7114
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IF 4.171
Kai Qian,Ting Tan,Hui Ouyang,Shi-Lin Yang,Wei-Feng Zhu,Rong-Hua Liu,Quan Wen,Yu-Lin Feng
A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification. The average molecular weight of PLP-1 was 16.2 kDa. Monosaccharide composition analysis showed that PLP-1 was composed of glucose as a glucan. The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-D-Glcp(1→ and →4)-β-D-Glcp(1→ with a molar ratio of 7.0 : 1.0. Moreover, the hypoglycemic activity of PLP-1 was investigated by palmitic acid and high glucose induced insulin resistant HepG2 cells. The results elucidated that PLP-1 could decrease the glucose concentration by up-regulating the expression of PI3K and AKT, and down-regulating the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells. Therefore, PLP-1 could serve as a dietary supplement to ameliorate insulin resistance for diabetic patients.
研究领域:
药理学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
ACS Applied Bio Materials
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2026
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IF 5.6
Yaxin He,Xin Xie,Yanghui Chen,Wenchen Tao,Junjie Yu,Meng Huang,Yan Lin,Juan Chen,Jian Liu,Xian Zhang
Adipose tissue metabolic dysfunction plays a central role in the development of obesity and related metabolic disease. Luteolin is a natural flavonoid with antioxidant, anti-inflammatory, antidiabetic, and antiobesity activities, while it is limited by poor solubility, bioavailability, and stability. This study developed a whey protein isolate (WPI)–Pueraria lobata amylopectin (PLA) composite gel, characterized by a dense 3D network and enhanced mechanical strength. The WPI-PLA gel efficiently encapsulated luteolin in a stable amorphous form and exhibited uniform particle size and excellent colloidal stability. This encapsulation improved the light, storage, and thermal stabilities of luteolin; enhanced its antioxidant activity due to improved dispersion; and enabled its preferential intestinal release. Encapsulated luteolin promoted the expression of thermogenic genes UCP1, Pgc-1α, Prdm16, and Cidea and protein levels of UCP1 and catalase in brown-differentiated C3H10T1/2 cells and inhibited the expression of lipopolysaccharide-induced inflammatory cytokines Il-6, Tnf-α, and Il-1β in RAW264.7 macrophages. In differentiated white 3T3-L1 adipocytes, WPI-LU-PLA restored insulin sensitivity impaired by macrophage-conditioned medium. This reversal was indicated by the enhanced expression of the insulin sensitivity-related genes GLUT4 and Irs1, along with increases in GLUT4 and p-Akt protein levels. In conclusion, the WPI-PLA composite gel enhances the stability, bioaccessibility, and bioactivity of luteolin, suggesting potential for functional foods targeting metabolic dysfunction in adipose tissues.
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DOI
PubMed
高影响因子
Food & Function
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2018
·Vol. 9
·期 5
·2644-2652
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IF 3.289
Can Xu,Ningbo Qin,Chunyan Yan,Shumei Wang
The root of Pueraria lobata is considered to be a medicinal and edible herb for the treatment of diabetes, and it has a long history of application in China. To explore the constituents responsible for the anti-hyperglycemic activities of P. lobata, a water-soluble polysaccharide (PL70-1-1) was isolated and purified by using a DEAE-Cellulose 52 anion exchange column and a Sephacryl S-100 gel filtration column. Its molecular weight (2584 Da) was determined by high performance gel permeation chromatography (HPGPC). Its structure was deduced by Fourier transform-infrared spectroscopy (FT-IR), monosaccharide composition analysis, gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). It was deduced that PL70-1-1 was a glucan, and its main chain consisted of (1→)-linked β-D-glucose, (1→4)-linked α-D-glucose, (1→4, 6)-linked β-D-glucose, and (1→3)-linked α-D-glucose, and the branch chain consisted of (1→)-linked β-D-glucose. The results of scanning electron microscopy showed that PL70-1-1 had a needle-like shape, and the surface had a scaly texture. The Congo red experiment showed that PL70-1-1 did not have a triple-helix structure. In addition, PL70 and PL70-1 displayed selective inhibitory effects on α-amylase and α-glucosidase in vitro. PL70 had remarkable α-glucosidase inhibitory activity. However, PL70-1-1 exhibited outstanding α-amylase inhibitory activity, with an IC50 of 3.945 μM in vitro. This indicated that its activity was 417 times higher than the positive control acarbose. PL70-1-1 may be beneficial as an α-amylase inhibitor, reducing the postprandial blood glucose level and treating type 2 diabetes.
研究领域:
药用植物学
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生物化学
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代谢疾病研究
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产品关联
DOI
PubMed
高影响因子
Foods
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2021
·Vol. 10
·期 6
·1320
·
IF 4.35
Junping Liu,Jiayan Zhang,Tao Liao,Lei Zhou,Liqiang Zou,Yafei Liu,Li Zhang,Wei Liu
The thermal inactivation kinetics of kudzu (Pueraria lobata) polyphenol oxidase (PPO) were investigated in model and food systems. PPO in kudzu tissue (tPPO) showed a higher thermostability than that of PPO in crude extract (cPPO) and purification fractions (pPPO). The PPO inactivation rate constant (k) increased with an increase in temperature, and tPPO showed the lowestkvalue, followed by that of cPPO and pPPO at the same temperature, indicating that PPO in the food system was more resistant to thermal treatment. Food constituents (pectin, starch, sucrose, and bovine serum albumin) in the food system decreased the activity of PPO but increased the thermostability of PPO, among which pectin exhibited the strongest protective effect against thermal inactivation, and the influence of sucrose was much slighter than that of other macromolecules. Fluorescence emission spectra indicated that pPPO exhibited stronger interactions with pectin than sucrose, and pPPO with pectin showed a more stable conformation under thermal treatment.Keywords:polyphenol oxidase;kudzu;thermostability;thermal inactivation kinetics;food constituents;conformation
研究领域:
食品科学
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酶学
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植物生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Junping Liu,Junmin Huang,Kaibo Yu,Xiaoyong Chang,Yangyang Li,Xiaowei Chen,Shengfeng Peng,Lei Zhou,Wei Liu
This study investigated the mechanism of browning inhibition by heat treatment (70 °C for 5 min) by analyzing its effects on polyphenol oxidase (PPO) activity, browning index, antioxidant activity, metabolite and gene expression profiles using physiological, metabolomic and transcriptomic approaches. Heat treatment inhibited PPO activity and delayed the browning of Pueraria lobata , while increasing free radical scavenging activity and total flavonoid content. Metabolomic analyses revealed that catechins, caffeic acid, tyrosine and coumaric acid might be the major metabolites associated with the browning of Pueraria lobata , whereas flavonoid and isoflavonoid metabolites might be beneficial for delaying browning. Multi-omics analysis revealed that the tyrosine metabolism and isoflavone biosynthesis pathways were enriched and played crucial regulatory roles in browning. This study provides a practical strategy for improving quality in post-harvest processing and deep processing of Pueraria lobata . Future research need focus on the spatial variation analysis of these browning-related metabolites in Pueraria lobata .
研究领域:
植物学
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食品科学与技术
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生物化学
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分子生物学
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采后生理学
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产品关联
DOI
PubMed
高影响因子
STARCH-STARKE
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2020
·
IF 2.226
Yuxue Zheng,Weixuan Sun,Wenhan Yang,Shiguo Chen,Donghong Liu,Jinhu Tian,Xingqian Ye
The study describes the effects of xanthan gum (0.5–2.0%) on the in vitro digestibility and rheological and pasting properties of kudzu ( Pueraria lobata ) starch pastes. The flow behavior index ( n ) shows a decrease in trends (from 0.43 to 0.31) as the xanthan gum concentration increases, indicating pseudoplastic behavior. Dynamic viscoelasticity analysis indicate that the elastic ( G ′) and viscous ( G ″) moduli increase in the presence of xanthan gum, suggesting a strong interaction between xanthan gum and kudzu starch in the composite system. All concentrations of xanthan gum result in increased elasticity ( G ′ > G ″). Pasting properties reveal that the peak and final viscosity of the xanthan gum–kudzu starch dispersion decreases with the increasing concentration of xanthan gum, highlighting the importance of xanthan gum in controlling overall viscosity. The addition of xanthan gum also decreases the hydrolysis of kudzu starch during in vitro digestion.
研究领域:
食品科学
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生物物理学
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营养学
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DOI
高影响因子
BIOLOGICAL & PHARMACEUTICAL BULLETIN
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2013
·
IF 1.849
Zhao-Bo Li,Chang-Fu Li,Jia Li,Yan-Sheng Zhang
As part of the efforts to understand isoflavonoid metabolism in Pueraria lobata at the molecular level, the cDNAs encoding two divergent 4-coumarate:coenzyme A ligases (4CLs, designated Pl4CL1 and Pl4CL2, respectively) were isolated from P. lobata roots. Sequence analysis revealed that Pl4CL1 had an N-terminal extension of twenty-one amino acid residues compared to Pl4CL2. Phylogenetic analysis showed that Pl4CL1 and Pl4CL2 fell into angiosperm Class II and Class I, respectively. Through in vitro biochemical assays, both Pl4CLs were found to have the capacity to utilize 4-coumarate and trans-cinnamate as substrates, while neither of them could convert sinapate. Pl4CL2 had a broader substrate specificity than Pl4CL1. The affinity of Pl4CL1 for 4-coumarate was 2.6-fold higher than that of Pl4CL2 (with the Km values of 3.5 µM and 9.1 µM, respectively). Combining the dataset including gene expression profiles, metabolites measurements, and biochemical properties, our results indicated that Pl4CL1, just as other angiosperm Class II 4CLs, might play a role in isoflavone biosynthesis in P. lobata, while Pl4CL2 belongs to angiosperm Class I, and may function as a housekeeping enzyme concerning lignification.
研究领域:
植物生物化学
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分子生物学
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植物生理学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）