Journal of Environmental Sciences
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2019
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IF 3.556
Meiqian Wang,Hua Yin,Hui Peng,Mi Feng,Guining Lu,Zhi Dang
The degradation of 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) by Pycnoporus sanguineus was investigated in order to explore the impact of the heavy metal Cu 2+ on BDE-47 decomposition and the subsequent formation of metabolites, as well as to further elucidate the degradation mechanism of BDE-47. An increase in degradation rate from 18.63% to 49.76% in the first four days and its stabilization at (51.26 ± 0.08)% in the following days of BDE-47 incubation were observed. The presence of Cu 2+ at 1 and 2 mg/L was found to promote the degradation rate to 56.41% and 60.79%, respectively, whereas higher level of Cu 2+ (≥ 5 mg/L) inhibited the removal of BDE-47. The similar concentration effects of Cu 2+ was also found on contents of fungal protein and amounts of metabolites. Both intracellular and extracellular enzymes played certain roles in BDE-47 transportation with the best degradation rate at 27.90% and 27.67% on the fourth and third day, individually. During the degradation of BDE-47, four types of hydroxylated polybrominated diphenyl ethers (OH-PBDEs), i.e., 6′-OH-BDE-47, 5′-OH-BDE-47, 4′-OH-BDE-17, 2′-OH-BDE-28, and two bromophenols, i.e., 2,4-DBP and 4-BP were detected and considered as degradation products . These metabolites were further removed by P. sanguineus at rates of 22.42%, 23.01%, 27.04%, 27.96%, 64.21%, and 40.62%, respectively.
研究领域:
环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
BIOTECHNOLOGY PROGRESS
·
2017
·Vol. 33
·期 5
·1314-1322
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IF 1.986
Chaohong Shi,Nengwu Zhu,Naixin Kang,Pingxiao Wu,Xiaoping Zhang,Yanhong Zhang
Biorecovery is emerging as a promising process to retrieve gold from secondary resources. The present study aimed to explore the uptake pattern of Pycnoporus sanguineus biomass for gold, identify the effective functional groups in gold recovery process, and thus further intensify the process via microbial surface modification. Results showed that P. sanguineus biomass could effectively recover gold with the formation of highly crystal AuNPs without any exogeneous electron donor. Under the conditions of various initial gold concentrations (1.0, 2.0, and 3.0 mM), biomass dosage of 2.0 g/L, solution pH value of 4.0, and incubation temperature of 30°C, the uptake equilibrium established after 4, 8, and 12 h, respectively. The uptake process could be well described by pseudo-second order kinetics model (R 2 = 0.9988) and Langmuir isotherm model (R 2 = 0.9958). The maximum uptake capacity of P. sanguineus reached as high as 358.69 mg/g. Further analysis indicated that amino, carboxyl and hydroxyl groups positively contributed to the uptake process. Among them, amino group significantly favored the uptake of gold during recovery process. When P. sanguineus biomass was modified by introduction of amino group, the gold uptake process was successfully intensified by shortening the uptake period and enhancing the uptake capacity. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1314–1322, 2017
研究领域:
环境生物学
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生物技术
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DOI
PubMed
高影响因子
Nanoscale Research Letters
·
2015
·Vol. 10
·期 1
·1-8
·
IF 2.779
Shi Chaohong,Zhu Nengwu,Cao Yanlan,Wu Pingxiao
The development of green procedure for the synthesis of gold nanoparticles (AuNPs) has gained great interest in the field of nanotechnology. Biological synthetic routes are considered to be environmentally benign and cost-effective. In the present study, the feasibility of AuNPs’ synthesis via intracellular protein extract (IPE) of Pycnoporus sanguineus was explored. The characteristics of generated particles of formation, crystalline nature, and morphology and dimension were analyzed by UV-vis spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM), respectively. UV-vis spectra exhibited strong absorption peaks in 520 to 560 nm, indicating the formation of AuNPs. XRD analysis revealed that the formed AuNPs were purely crystalline in nature. TEM observation showed that AuNPs with various shapes including spherical, pseudo-spherical, triangular, truncated triangular, pentagonal, and hexagonal, ranging from several to several hundred nanometers, were synthesized under different conditions. The average size of AuNPs decreased from 61.47 to 29.30 nm as the IPE addition increased from 10 to 80 mL. When the initial gold ion concentration changed from 0.5 to 2.0 mM, the average size rose from 25.88 to 51.99 nm. As in the case of solution pH, the average size was 84.29 nm with solution pH of 2.0, which diminished to 6.07 nm with solution pH of 12.0. Fourier transform infrared (FTIR) analysis implied that the functional groups including hydroxyl, amine, and carboxyl were involved in the reduction of gold ions and stabilization of AuNPs. The catalysis results showed that 0.019 mg of AuNPs with average size of 6.07 nm could catalyze the complete degradation of 12.5 μmol of 4-nitroaniline within 6 min and the degradation rate increased drastically with the addition of AuNPs. All the results suggested that the IPE of P. sanguineus could be potentially applied for the eco-friendly synthesis of AuNPs.
研究领域:
纳米技术
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环境科学
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生物学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2017
·
IF 3.743
Mi Feng,Hua Yin,Hui Peng,Xintong Liu,Pingping Yang,Guining Lu,Zhi Dang
Simultaneous TBBPA removal and Cr(VI) reduction by Pycnoporus sanguineus together with the effect of these co-existed pollutants on the fungal cellular characteristics were investigated in this study, aiming at illuminating the mechanism involved in the interactions between contaminants and microbial cells. The results revealed that Cr(VI) reduction and TBBPA removal declined from 92.5%, 75.4–30.6%, 44.8% when Cr(VI) concentration increased from 5 to 40 mg/L, respectively. The removal efficiencies for Cr(VI) and TBBPA reached 61.4% and 94% separately under the optimum concentration of TBBPA at 10 mg/L. Subsequent analyses indicated that the negative effect of Cr(VI) of high concentrations on Cr(VI) reduction and TBBPA removal was mainly attributed to the inhibition of fungal growth, intracellular proteins synthesis , cell viability and ATP enzyme activity . Compared with the moderate impact of TBBPA, the cell membrane of P. sanguineus was impaired severely and the surface morphology and intracellular structure changed dramatically in the presence of high concentration of Cr(VI) (above 10 mg/L). This study also suggested that high level of TBBPA (15 and 20 mg/L) promoted the synthesis of intracellular proteins and improved ATP enzyme activity within the first 48 h of the reaction for enhancing the transportation and transformation of TBBPA.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）