Inhibitory effects (IEs) of flavonoids from Rosa roxburghii and R. sterilis fruits against α-glucosidase were investigated. Crude extract of R. roxburghii fruits ( Rr CE) and R. sterilis fruits ( Rs CE) were extracted with ethanol. The n-butanol extraction of aqueous Rr CE dispersion ( Rr CE - B) showed the greatest inhibition of α-glucosidase. The most abundant three components in Rr CE - B were kaempferol hexose, rutin, and catechin (Cat). The predominant active compounds were characterized as flavonoids by UV–vis spectra. Combination analysis suggested that Rr CE was a favorable choice to combine with acarbose (Aca). After simulated gastric digestion, IEs of flavonoids decreased greatly, especially when sodium nitrite was supplemented in the simulated gastric fluid (SGF). The UV–vis spectra indicated that flavonoids were basically stable in the SGF without sodium nitrite, but were oxidized by the HNO 2 , a natural component in the gastric fluid. The results of this study will help food scientists design special foods for diabetic patients.
Jing-Yu He,Yuan-He Zhang,Na Ma,Xu-Lin Zhang,Meng-Hua Liu,Wei-Ming Fu
The functional and potential bioactive ingredients, including the elements, amino acids and phytochemicals, in Rosa roxburghii (RR) and R. sterilis (RS) fruits were analysed by ICP-OES and -MS, HPLC-UV, and LC-MS/MS, respectively, and were compared to elucidate the characteristics of their ingredients. The antioxidant activities of the ethanolic extracts of RR and RS fruits were evaluated using four different methods, including 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, hydroxyl radical scavenging activity, inhibition of β -carotene bleaching, and ferric reducing antioxidant power assays; the correlation between the ingredients and their antioxidant activities was assessed. Furthermore, principal component analysis was selected to differentiate between the intra- and interspecies variation of RR and RS fruits. The results showed that RR and RS fruits contained numerous nutritious and biological ingredients and exhibited antioxidant activities, but there were significant differences among the fruits. Nevertheless, RR and RS fruits are attractive materials to produce highly valuable, functional foods.
危险品化学品经营许可证（带存储）