Frontiers in Plant Science
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2022
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IF 6.627
Zhao Lu,Song Zhongbang,Wang Bingwu,Gao Yulong,Shi Junli,Sui Xueyi,Chen Xuejun,Zhang Yihan,Li Yongping
Proanthocyanidins (PAs) are important phenolic compounds and PA biosynthesis is regulated by a ternary MBW complex consisting of a R2R3-MYB regulator, a bHLH factor and a WDR protein. In this study, a tobacco R2R3-MYB factor NtMYB330 was characterized as the PA-specific regulator in which the PA biosynthesis was promoted in the flowers of NtMYB330-overexpressing lines while decreased in the flowers of ntmyb330 mutants. NtMYB330 can interact with flavonoid-related bHLH partner NtAn1b and WDR protein NtAn11-1, and the NtMYB330-NtAn1b complex is required to achieve strong transcriptional activation of the PA-related structural genes NtDFR1, NtANS1, NtLAR1 and NtANR1. Our data reveal that NtMYB330 regulates PA biosynthesis in seeds and affects seed germination, in which NtMYB330-overexpressing lines showed higher PA accumulations in seed coats and inhibited germination, while ntmyb330 mutants had reduced seed coat PAs and improved germination. NtMYB330 affects seed germination possibly through two mechanisms: modulating seed coat PAs to affect coat-imposed dormancy. In addition, NtMYB330 regulates the expressions of abscisic acid (ABA) and gibberellin acid (GA) signaling-related genes, affecting ABA-GA crosstalk and seed germination. This study reveals that NtMYB330 specifically regulates PA biosynthesis via formation of the MBW complex in tobacco flowers and affects germination through adjustment of PA concentrations and ABA/GA signaling in tobacco seeds.
研究领域:
植物分子生物学
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转录调控
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种子生理学
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DOI
PubMed
高影响因子
PLANT MOLECULAR BIOLOGY REPORTER
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2025
·Vol. 44
·期 1
·21
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IF 1.4
Fan Huiyan,Zhou Jingzhi,Ying Yiling,Tang Kaijie,Han Jiayi,Wang Qiuying,Kai Guoyin
Background (TF) R2R3-MYB transcription factors (TFs) play crucial roles in regulating plant secondary metabolism, which is vital for growth, development, and stress responses. Salvia miltiorrhiza , a medicinal plant, is known for its bioactive compounds such as salvianolic acids. Understanding the R2R3-MYB TF family in S. miltiorrhiza could provide insights into the regulation of secondary metabolism and contribute to quality improvement in this important medicinal plant. Methods and Results We conducted a comprehensive analysis of the R2R3-MYB TF family in S. miltiorrhiza , identifying 431 MYB-like TFs, including 149 R2R3-MYB TFs. Phylogenetic analysis classified these TFs into 27 distinct groups. Chromosomal localization analysis revealed their widespread distribution across all chromosomes. Structural analysis showed that most R2R3-SmMYB genes contain untranslated regions (UTRs) and conserved motifs, with motif5 potentially playing a key role in differentiation. Interspecific collinearity analysis demonstrated the highest collinearity between S. miltiorrhiza and Salvia baicalensis , with 12 intraspecific collinear genes exhibiting interspecific collinearity with multiple species. Promoter analysis identified abundant light- and hormone-responsive elements, particularly MeJA-responsive elements. Expression profiling highlighted SmMYB50 as highly responsive to MeJA, ABA, and YE induction, promoting salvianolic acid accumulation. Transgenic validation confirmed that SmMYB50 activates key biosynthetic genes such as SmCYP98A14 , crucial for SalB synthesis. Conclusions Our findings provide valuable insights into the functional roles of R2R3-SmMYB-TFs in regulating secondary metabolism in S. miltiorrhiza . SmMYB50 was identified as a key regulator, offering new directions for future research on metabolic pathways and quality improvement in this important medicinal plant.
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高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
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2023
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IF 6.5
Junjie Yang,Jin Xu,Yingting Zhang,Jiebing Cui,Hailiang Hu,Jinyu Xue,Lijuan Zhu
As the most abundant renewable energy source , wood comprises the secondary cell wall (SCW). SCW biosynthesis involves lignin and cellulose deposition. Increasing studies have illustrated that R2R3-MYB transcription factors (TFs) play pivotal roles in affecting lignin accumulation and SCW formation. Nevertheless, the regulatory roles of R2R3-MYBs are still unresolved in Cryptomeria fortunei Hooibrenk cambium and wood formation. To dissect the potentials of CfMYBs , we successfully cloned and intensively studied the functions of CfMYB4 and CfMYB5 in SCW formation and abiotic stress response. They both contained the conserved MYB domain capable of forming a special structure that could bind to the core motifs of downstream genes. The phylogenetic tree implied that two CfMYBs clustered into different evolutionary branches. They were predominantly expressed in the stem and were localized to the nucleus. Furthermore, CfMYB4 functioned as an activator to enhance lignin and cellulose accumulation, and increase the SCW thickness by elevating the expression levels of SCW-related genes. By contrast, CfMYB5 negatively regulated lignin and cellulose biosynthesis, and decreased SCW formation by reducing the expression of SCW biosynthetic genes. Our data not only highlight the regulatory functions of CfMYBs in lignin deposition but also provide critical insights into the development of strategies for the genetic improvement of Cryptomeria fortunei wood biomass.
研究领域:
植物生物学
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分子遗传学
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林学
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PubMed
高影响因子
BMC PLANT BIOLOGY
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2023
·Vol. 23
·期 1
·1-16
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IF 5.3
Zhang Yanan,Wang Di,Li Hui,Bai Hongtong,Sun Meiyu,Shi Lei
Background Lavender (genus Lavandula, family Lamiaceae) is an aromatic plant widely grown as an ornamental plant. The chemical composition of lavender is characterized by monoterpenoids, sesquiterpenoids, and other compounds, which are primarily synthesized and stored in epidermal secretory structures called glandular trichomes (GTs). Volatile organic compounds (VOCs) are responsible for the aroma characteristics of plant oil that drive consumer preference. Aroma is usually regarded as a characteristic trait for the classification of aromatic plants. Interestingly, VOCs are synthesized and stored in GTs. Lamiaceae species such as purple perilla, peppermint, basil, thyme, and oregano usually possess two types of GTs: peltate glandular trichomes (PGTs) and capitate glandular trichomes (CGTs). But the development process of PGTs in lavender has been reported in only a few studies to date.Results In this study, we identified and quantified the VOCs in four lavender cultivars by headspace-solid phase micro extraction-gas chromatography mass spectrometry (HS–SPME–GC–MS). A total of 66 VOCs were identified in these four cultivars, the most prominent of which were linalyl acetate and linalool, and flowers were the main site of accumulation of these VOCs. Here, we examined the developmental process of PGTs, including the formation of their base, body, and apex. The apex cells contained secretory cavities, which produced VOCs. Based on the reference genome sequence of the lavender cultivar ‘Jingxun 2’, several R2R3-MYB subfamily genes related to GT formation were identified. These results will guide the engineering of GTs and molecular breeding of lavender for improving the VOC content.Conclusion sIn this study, we identified the VOCs in four lavender cultivars. We analyzed the formation of GTs, and compared the number and diameter size of PGTs among four lavender cultivars. Additionally, we identified four candidate genes belonging to the R2R3-MYB family.
研究领域:
植物学
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分子生物学
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芳香植物研究
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PubMed
高影响因子
Plants-Basel
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2025
·Vol. 14
·期 22
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IF 4.1
Dong Li,Xumin Wang,Yujiao Song,Jianchao Sun,Shuhan Yu,Bowei Zhu,Xin Liu,Guodong Zhao,Tongsheng Zhao,Limin Wang
Seed oil content and fatty acid (FA) composition collectively determine the quality and economic value ofBrassica napus. Little is known about the role of R2R3-MYB transcription factors (TFs) in regulating FA biosynthesis inB. napus. Here,BnaC04.MYB89was found to be expressed primarily in developing seeds. Overexpression ofBnaC04.MYB89consistently decreased FA levels, as evidenced by its effect in both theArabidopsis thaliana myb89-1mutant andB. napusseeds. RNA-seq of developing seeds at 30 DAP (days after pollination) revealed marked suppression of FA biosynthetic genes inBnaC04.MYB89-overexpressing plants compared to the K407 control. ChIP (Chromatin immunoprecipitation) analysis revealed that BnaC04.MYB89 directly inhibited the expression ofBnaA03.BCCP1andBnaC03.HDwhile indirectly regulating that ofBnaA09.BADC1,BnaA03.BADC3,BnaA03.MOD1, andBnaA08.FAT8, thereby reducing seed FA accumulation. Collectively, these results elucidate the role for BnaC04.MYB89 and provide new insights into the transcriptional regulatory network controlling seed oil accumulation inB. napus.
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PubMed
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
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2021
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IF 4.27
Yu-Feng Xie,Rong-Xiang Zhang,Li-Jun Qin,La-la Song,De-Gang Zhao,Zhong-min Xia
MYB proteins play a crucial role in plant growth and development and stress responses. In this study, 160 members of the MYB gene family from the pepper genome database were used to analyze gene structures, chromosome localization, collinearity, genetic affinity and expression in response to heavy metals . The results identified R2R3-MYB members and further phylogenetically classified them into 35 subgroups based on highly conserved gene structures and motifs. Collinearity analysis showed that segmental duplication events played a crucial role in the functional expansion of the CaMYB gene family by intraspecific collinearity, and at least 12 pairs of CaMYB genes existed between species prior to the differentiation between monocots and dicots . Moreover, the upstream CaMYB genes were mainly localized to the phytohormone elements ABRE and transcription factor elements MYB and MYC. Further analysis revealed that MYB transcription factors were closely associated with a variety of abiotic stress-related proteins (e.g., MAC-complex and SKIP). Under the stress of five metal ions, Cd 2+ , Cu 2+ , Pb 2+ , Zn 2+ , and Fe 3+ , the expression levels of some CaMYB family genes were upregulated. Of these genes, pairing homologous 1 ( PH-1 ), PH-13 , and PH-15 in the roots of Capsicum annuum were upregulated to the greatest extent, indicating that these three MYB family members are particularly sensitive to these five metals. This study provides a theoretical reference for the analysis of the molecular regulatory mechanism of MYB family genes in mediating the response to heavy metals in plants. This study reveals the mode of interaction between MYB and a variety of abiotic stress proteins and clarifies the biological functions of CaMYB family members in the regulation of heavy metal stress.
研究领域:
植物学
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遗传学
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环境科学
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DOI
PubMed
高影响因子
Journal of Advanced Research
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2020
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IF 6.992
Xiaolong Hao,Zhongqiang Pu,Gang Cao,Dawei You,Yang Zhou,Changping Deng,Min Shi,Shivraj Hariram Nile,Yao Wang,Wei Zhou,Guoyin Kai
Salvia miltiorrhiza Bunge is an herb rich in bioactive tanshinone and salvianolic acid compounds. It is primarily used as an effective medicine for treating cardiovascular and cerebrovascular diseases. Liposoluble tanshinones and water-soluble phenolic acids are a series of terpenoids and phenolic compounds, respectively. However, the regulation mechanism for the simultaneous promotion of tanshinone and salvianolic acid biosynthesis remains unclear. This study identified a R2R3-MYB subgroup 20 transcription factor (TF), SmMYB98 , which was predominantly expressed in S. miltiorrhiza lateral roots. The accumulation of major bioactive metabolites, tanshinones, and salvianolic acids, was improved in SmMYB98 overexpression (OE) hairy root lines, but reduced in SmMYB98 knockout (KO) lines. The qRT-PCR analysis revealed that the transcriptional expression levels of tanshinone and salvianolic acid biosynthesis genes were upregulated by SmMYB98-OE and downregulated by SmMYB98-KO . Dual-Luciferase (Dual-LUC) assays demonstrated that SmMYB98 significantly activated the transcription of SmGGPPS1 , SmPAL1 , and SmRAS1 . These results suggest that SmMYB98-OE can promote tanshinone and salvianolic acid production. The present findings illustrate the exploitation of R2R3-MYB in terpenoid and phenolic biosynthesis, as well as provide a feasible strategy for improving tanshinone and salvianolic acid contents by MYB proteins in S. miltiorrhiza .
研究领域:
植物生物技术
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生药学
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分子生物学
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转录调控
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DOI
PubMed
高影响因子
Crop Journal
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2025
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IF 5.6
Yunlong Pang,Shanshan Li,Qiang Yan,Pingping Zhang,Yu Lu,Cunyuan Zhao,Tao Li,Hailiang Zhao,Shanyi Sun,Tingting Zhou,Xiaoqian Wang,Huaqiang Zhu,Yong Li,Lingrang Kong,Guihua Bai,Shubing Liu,Yuye Wu
Wheat grains contain various bioactive substances, of which, condensed tannins (CT) are polymeric flavan-3-ols that accumulate in wheat seed coat influencing the end-use quality and nutritional value. However, the genetic architecture underlying CT biosynthesis in wheat grain remains unclear. Here, we studied the deposition and genetic regulation of CT in wheat grains, and found that CT deposited specifically in the testa layer of red-grained wheat as catechin- and epicatechin-formed polymers. Genome-wide association study identified 22 genetic loci affecting CT content, one of which, TaTAN , a single dominant gene controlling CT presence, was mapped to chromosome 3A in a segregation population. Further pan-genome analysis, transcriptome profiling and ethyl methanesulfonate induced mutants sequencing revealed a R2R3-MYB transcription factor, TaMYB10-3A , as the causal gene . Three loss-of-function alleles in TaMYB10-3A caused by large fragment inversion-deletion and insertion were identified which abolish both CT deposition and red pigmentation, demonstrating the pleiotropic effect of TaMYB10-3A on CT presence and grain color. TaMYB10-3A directly trans -activates core flavonoid genes such as chalcone synthase and dihydroflavonol 4-reductase to initiate CT biosynthesis. Our investigation provides a comprehensive understanding of CT presence in wheat grains and lays a solid foundation for manipulating CT metabolites to improve wheat grain end-use quality and nutrition values in wheat.
研究领域:
作物遗传学
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植物生物化学
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分子生物学
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功能基因组学
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代谢调控
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谷物品质改良
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植物次生代谢
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高影响因子
危险品化学品经营许可证（带存储）