FOOD CONTROL
·
2020
·
IF 4.258
Xuefei Shao,Baocai Xu,Hui Zhou,Conggui Chen,Peijun Li
The mechanism of decreasing N- nitrosodimethylamine (NDMA) by Lactobacillus pentosus R3 isolated from Chinese dry sausage was assessed in a model system. The model system was devised using a DeMan-Rogosa and Sharp-ferrous sulfate broth supplement with NDMA or its precursors. The results showed that inoculating L. pentosus R3 could significantly decrease the content of NDMA ( P < 0.05). The NDMA precursors (dimethylamine and putrescine) were found to be decreased by L. pentosus R3 ( P < 0.05). Nitrite was degraded to nitrosonium cation by acidolysis, which may promote the formation of NDMA. Direct degradation of NDMA by L. pentosus R3 was also observed. The strain exhibited antioxidant activities , including Fe 2+ chelating activity, which may be responsible for inhibiting the formation reaction and decreasing the precursors of NDMA. Thus, L. pentosus R3 decreased the content of NDMA possibly by three ways: inhibition of the formation reaction, decrease in precursors (DMA and putrescine) and direct degradation. It is interesting to understand the mechanism of decreasing NDMA by L. pentosus R3 to improve the safety of fermented meat products .
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Chenyang Zhang,Zhizhen Wang,Long Yuan,Ruixia Ti,Huarui Wu,Hongming Yuan
Research on double perovskites R 2 NiMnO 6 with small R 3+ cations encounters challenges in following aspects: Firstly, stabilizing significant structural distortions is relatively difficult, and minimizing defects such as mixed oxidation states and antisite disorder also poses challenges owing to thermodynamic considerations. Secondly, the theoretically predicted ↑↑↓↓ spin alignment, arising from competition between nearest-neighboring ferromagnetic (NN FM) and next-nearest-neighboring antiferromagnetic (NNN AFM) interactions, remains controversial. In this study, double perovskite Lu 2-x Y x NiMnO 6 samples were synthesized via a hydrothermal method. Compared to other synthesis methods, the hydrothermal method promotes the stabilization of Ni 2+ and Mn 4+ ions, and stabilizes structural distortion for R 2 NiMnO 6 with small R 3+ cations. High angle annular dark field scanning transmission electron microscopy (HAADF-STEM) images revealed a rock-salt arrangement of Ni 2+ and Mn 4+ ions. By the variation in the R 3+ ionic radius, the magnetic interactions were continuously tuned. Compared to R MnO 3 and R 2 CoMnO 6 , the less pronounced deviation from the expected trend in the magnetic ordering temperature with the R 3+ ionic radius highlighted the significant role of orbital ordering on enhancing the relative strength of NNN AFM with respect to NN FM interactions.
研究领域:
材料科学
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固体物理
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无机化学
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纳米技术
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凝聚态物理
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产品关联
DOI
高影响因子
ANNALS OF MICROBIOLOGY
·
2017
·Vol. 68
·期 1
·27-34
·
IF 1.122
Xiao Yaqing,Li Peijun,Xu Mei,Wang Wu,Chen Conggui
The objective of this study was to evaluate the ability of five strains of meat-borne bacteria to decrease N-nitrosodimethylamine (NDMA) and N-nitrosodiethylamine (NDEA) and to elucidate the mechanism in Mann-Rogosa-Sharp (MRS) broth. Lactobacillus pentosus R3 was found to be the most effective in decreasing the concentration of the two N-nitrosamines (NAs) in MRS broth, with a rate of 22.05% for NDMA and 23.31% for NDEA. The concentration of the two NAs could not be reduced by either extracellular metabolites or intracellular extracts of Lb. pentosus R3 (P > 0.05), and proteinaceous substances in the cell debris were found to be responsible for the decrease. These were surface-layer proteins (SLPs) located on the cell wall. Therefore, the decrease in NDMA and NDEA by Lb. pentosus R3 is associated with its SLPs. Lb. pentosus R3 may be developed as a starter culture in the production of fermented foods with lower NAs.
研究领域:
食品科学
·
微生物学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2025
·
IF 6.8
Qiang Li,Cunrui Li,Yuanmin Chang,Mingzhi Su,Rilei Yu,Zhangjian Huang,Yue-Wei Guo,Xin Jin
The inhibition of Rho-associated coiled-coil kinase (ROCK) has emerged as a promising strategy for reducing intraocular pressure (IOP) and treating glaucoma. Here, we report the synthesis and evaluation of novel ROCK inhibitors, D25 and R3, which were designed to optimize selectivity, efficacy, and ocular bioavailability. D25 potently inhibited ROCK1/2 with IC50 values of 47.2 nM and 33.8 nM, respectively, surpassing Netarsudil. Compound R3 had weaker ROCK inhibition but demonstrated favorable lipophilicity (logP) and good selectivity to ROCKs, which enhances its potential and safety for ocular delivery. In human trabecular meshwork (HTM) cells, R3 showed lower cytotoxicity than Netarsudil and effectively mitigated oxidative damage, enhanced cellular integrity, and reduced inflammatory cytokine secretion. In rabbit models, D25 significantly lowered IOP, outperforming (S)-Netarsudil. R3 exhibited weaker IOP-lowering efficacy but better selectivity. D25 is a promising glaucoma treatment candidate, with R3 as a safer alternative for further optimization.
研究领域:
药理学
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眼科
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2019
·Vol. 20
·期 3
·487
·
IF 4.183
Xuexia Li,Xiubo Du,Jiazuan Ni
Alzheimer’s disease (AD) is a neurodegenerative disease with high morbidity that has received extensive attention. However, its pathogenesis has not yet been completely elucidated. It is mainly related to β-amyloid protein deposition, the hyperphosphorylation of tau protein, and the loss of neurons. The main function of tau is to assemble tubulin into stable microtubules. Under pathological conditions, tau is hyperphosphorylated, which is the major component of neurofibrillary tangles (NFT) in AD. There is considerable evidence showing that the dyshomeostasis of Zn2+is closely related to the development of AD. Herein, by using the third repeat unit of the microtubule-binding domain of tau (tau-R3), we investigated the effect of Zn2+on the aggregation and neurotoxicity of tau. Experimental results showed that tau-R3 probably bound Zn2+via its Cys residue with moderate affinity (association constant (Ka) = 6.82 ± 0.29 × 104M−1). Zn2+accelerated tau-R3 aggregation and promoted tau-R3 to form short fibrils and oligomers. Compared with tau-R3, Zn2+-tau-R3 aggregates were more toxic to Neuro-2A (N2A) cells and induced N2A cells to produce higher levels of reactive oxygen species (ROS). The dendrites and axons of Zn2+-tau-R3-treated neurons became fewer and shorter, resulting in a large number of neuronal deaths. In addition, both tau-R3 and Zn2+-tau-R3 aggregates were found to be taken up by N2A cells, and more Zn2+-tau-R3 entered the cells compared with tau-R3. Our data demonstrated that Zn2+can aggravate tau-R3 aggregation and neurotoxicity, providing clues to understand the relationship between Zn2+dyshomeostasis and the etiology of Alzheimer’s disease.Keywords:tau-R3;Zn2+;aggregation;neurotoxicity
研究领域:
神经科学
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分子生物学
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病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Inorganic Chemistry Frontiers
·
2024
·Vol. 11
·期 17
·5648-5656
·
IF 6.1
Yuhan Hu,Xi Xu,Ruixi Wang,Jingyun Han,Shunsong Zhang,Shuhui Zhan,Jingyu Guo,Li-Ming Wu,Ling Chen
Tin(II)-containing phosphates are promising candidates for designing and studying materials with enhanced birefringence. Herein, we present a novel compound [Sn3OF]PO4 that exhibits significant birefringence (Δnobv. = 0.117 at 546 nm), a five-fold improvement compared to that of the related compound [Sn3F3]PO4 discovered nearly 40 years ago. The remarkable enhancement in birefringence is primarily attributed to the anisotropic [SnO3F]5− structure building unit. By simple regulation of the F/O ratio, the undesirable R3 symmetry in [Sn3F3]PO4 is disrupted. This regulation also realigns the lone pairs, resulting in substantial optical anisotropy.
研究领域:
材料科学
·
光学
✓ 已验证
DOI
高影响因子
Frontiers in Cell and Developmental Biology
·
2021
·
IF 6.684
Song Yi-Sheng,Dai Ming-Zhu,Zhu Chen-Xia,Huang Yan-Feng,Liu Jing,Zhang Cheng-Da,Xie Feng,Peng Yi,Zhang Yong,Li Chun-Qi,Zhang Li-Jiang
The zebrafish as an alternative animal model for developmental toxicity testing has been extensively investigated, but its assay protocol was not harmonized yet. This study has validated and optimized the zebrafish developmental toxicity assay previously reported by multiple inter-laboratory studies in the United States and Europe. In this study, using this classical protocol, of 31 ICH-positive compounds, 23 compounds (74.2%) were teratogenic in zebrafish, five had false-negative results, and three were neither teratogenic nor non-teratogenic according to the protocol standard; of 14 ICH-negative compounds, 12 compounds (85.7%) were non-teratogenic in zebrafish and two had false-positive results. After we added an additional TI value in the zebrafish treated with testing compounds at 2 dpf along with the original 5 dpf, proposed a new category as the uncategorized compounds for those TI values smaller than the cutoff both at 2 dpf and 5 dpf but inducing toxic phenotypes, refined the testing concentration ranges, and optimized the TI cut-off value from ≥ 10 to ≥ 3 for compounds with refined testing concentrations, this optimized zebrafish developmental assay reached 90.3% sensitivity (28/31 positive compounds were teratogenic in zebrafish) and 88.9% (40/45) overall predictability. Our results from this study strongly support the use of zebrafish as an alternative in vivo method for screening and assessing the teratogenicity of candidate drugs for regulatory acceptance.
研究领域:
毒理学
·
药理学
·
发育生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2019
·
IF 2.291
Peixi Hao,Linwen Jiang,Hanlei Zhao,Jiangtao Li,Shiwen Qiu,Hongbing Chen
The MFeO 3 : R 3+ (M = La, Gd; R = Eu, Er, Ho) nanocrystalline powders were successfully synthesized by the sol–gel combustion method using glycine-based gel at a low ignition temperature. The as-burnt products present honeycomb-like structure with many irregular continuous pores due to the releasing of a large amount of gas during the combustion reaction process. The lattice parameters and cell volumes of MFeO 3 :R 3+ (M = La, Gd; R = Eu, Er, Ho) nanocrystalline powders are compared in detail. The magnetic properties of all the samples were studied systematically. The results show that the doping of rare-earth ions enhanced greatly the coercivity of GdFeO 3 and LaFeO 3 as a result of the enhancement of magnetic anisotropy . The influence of doped rare-earth ions on the magnetic properties was summarized. The distinction of magnetism of MFeO 3 :R 3+ (M = La, Gd; R = Eu, Er, Ho) were explained in detail by the intrinsic magnetism of doped rare-earth ions. This work is significant for the application of rare-earth elements in other magnetic materials.
研究领域:
材料科学
·
磁学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）