MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 8
·1-10
·
IF 6.408
Zhao Tingting,Yan Weizhen,Dong Fengqi,Hu Xinlong,Xu Yanli,Wang Zhenyu,Shen Yating,Wang Wanrong,Zhao Ye,Wei Wenmei
COVID-19 necessitates the development of reliable and convenient diagnostic tools. In this work, a facile 3D-printed smartphone platform was constructed that achieved reliable visual detection of SARS-CoV-2 by eliminating the effect of ambient light and fixing the camera position relative to the sample. The oligonucleotide probe is modified with orange-red-emitting TAMRA working as an internal standard and green-emitting FAM serving as a sensitive sensing agent. Under 365-nm UV excitation, the emission wavelengths of TAMRA and FAM are 580 nm and 518 nm, respectively. When the probes interact with the targets, the green fluorescence gradually restores while the orange-red fluorescence remains stable. Thus, a striking color transition from orange-red to green could be observed by the naked eye. The detection limit of SARS-CoV-2 nucleic acid is 0.23 nM, and the entire process of color change could be completed in 25 min. Furthermore, the RGB value analysis of the sample solution was conducted using a smartphone for reliable and reproducible discrimination of SARS-CoV-2. The proposed smartphone platform might establish a general method for visual detection of SARS-CoV-2 nucleic acid as well as other virus-related diseases. Graphical abstract
研究领域:
生物医学工程
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分子生物学
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诊断工具
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病毒学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Ming-Qin He,Hong-Wei Li,Yuqing Wu
Nitrite is a commonly used food preservative and a water contaminant that has garnered significant attention due to its harmful effects on human health. Developing a simple and sensitive method for determining nitrite levels is crucial for safeguarding public health. In this paper, we present a novel ratiometric fluorescent nanosensor (CDs@UiO-66-NH 2 ), created by combining orange-red-emitting CDs with blue-emitting UiO-66-NH 2 . This ratiometric probe detects nitrite ions (NO 2 − ) based on the diazotization reaction between the amino group in UiO-66-NH 2 and the target NO 2 − , where the blue emission of UiO-66-NH 2 is quenched but the orange-red emission of CDs remains stable. The probe demonstrated a detection range of 0.5–20 μM with a limit of detection (LOD) of 0.157 μM for NO 2 − . Due to the probe’s distinct color changes in response to NO 2 − , RGB values can be easily read using a smartphone, enabling ultrasensitive visual detection of NO 2 − with an LOD of 0.76 μM. This sensor was successfully applied to detect NO 2 − in environmental water samples. Finally, a smartphone-based RGB reading method using CDs@UiO-66-NH 2 for visual quantitative detection of NO 2 − was proposed, broadening the application of CDs@UiO-66-NH 2 in environmental protection.
研究领域:
分析化学
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环境科学
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材料科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 21
·3388
·
IF 5.561
Xijuan Tu,Yunmin Tao,Jiaxu Chen,Chunping Du,Qian Jin,Yuchang He,Ji Yang,Shaokang Huang,Wenbin Chen
In the present study, a lanthanide fluorescence sensor array was developed for the discrimination of honey’s botanical origin. Dipicolinic acid (DPA) was used as the antenna ligand for sensitizing the fluorescence of Tb3+and Eu3+to prepare the DPA-Tb3+/Eu3+complex. This lanthanide fluorescence sensor showed a cross-reactive response to the major constituents of honey, which led to the result that different classes of honey solution exhibited distinct quenching effects on the fluorescence of the DPA-Tb3+/Eu3+complex. Furthermore, a fluorescence sensor array composed of ten sensors was constructed by adjusting the pH and the component of the DPA-Tb3+/Eu3+complex to show multivariate responses towards honey. The visual fluorescence image of the sensor array was recorded by using a smartphone under excitation with portable UV lamp. Results indicated that the pattern of the visual image was related with the botanical origin. After extracting the RGB value of each sensor in 96-well plate, the ratio of R/G was used for principal component analysis (PCA). The results showed that three classes of honey (astragalus, logan, and litchi) were well distinguished. Moreover, the value of principal component 1 (PC1) showed good linearity with the composition of mixing honey and could be used for semi-quantitative analysis. The proposed lanthanide fluorescence sensor array presents a visual and portable method for the discrimination of a honey’s origin without the use of analytical instruments, and might provide a novel and simple strategy for the measurement of food origin.Keywords:honey;botanical origin;lanthanide complex;fluorescence;sensor array
研究领域:
食品科学
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分析化学
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光谱学
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材料科学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Wen-Jie Wu,Qi Zhao,Rui Zhou,Ya-Chuan Liang,Wen-Bo Zhao,Chong-Xin Shan
An europium functionalized ZnO quantum dots (QDs) ratiometric fluorescent nanoprobe is designed to establish a real time, on-site visual, and highly sensitive probe method for tetracycline (TC). The yellow-emitting ZnO QDs serves as the internal reference, while the Eu 3+ chelated on the surface of ZnO QDs is used as the signal reporting unit. This nanoprobe exhibits rapid response, excellent selectivity, and high sensitivity with a detection limit of 4 nM in detecting the levels of TC. In addition, fluorescence of the nanoprobe can change from yellow to red as the concentration of TC increases. Thus, naked eye detection of TC was realized using the test paper processed by nanoprobe, followed by RGB value analysis function on the mobile phone APP.
研究领域:
分析化学
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纳米技术
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环境科学
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生物技术
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Lingling Qin,Jie Cao,Dan Lin,Shihao Xu,Yucheng Li,Changlong Jiang
Utilization of organic reactions can offer more counterparts to design organic fluorescent probes and expand the probe libraries. Herein, a Strecker-like reaction was induced to prepare fluorescent probe for enzyme-free and visual quantitative monitoring of carbamates. Carbamate pesticides (CPs) are representative pesticides whose residues in water bodies and agricultural and sideline products seriously endanger human health. It is particularly important to develop a real-time/on-site sensing method to monitor the residue levels of CPs. The bottleneck of traditional detection methods is that only one of the pesticides can be selectively detected, so the full-spectrum detection of CPs can improve the practical application value. Based on the principle of Strecker-like reaction induced by 2, 3-naphthalenedialdehyde (NDA) and sulfite, we constructed an enzyme-free ratiometric fluorescent probe with CdTe quantum dots (CdTe QDs) as the background fluorescence for full-spectrum visual recognition of CPs. After addi-tion of CPs, isoindole with green fluorescence is generated by nucleophilic condensation reaction, and this fluorescent probe provides a distinct color variation from red to green, which shows a good linear relationship to CPs residues concentrations in the range of 0.1–4.5 μM with a sensitive limit of detection (LOD) of 18.6 nM. In addition, we developed a novel enzyme-free ratiometric fluorescence intelligent detection platform combined with a smartphone, which can visually quantitatively detect CPs in water, apple, and cabbage by identifying the RGB values of the fluorescent probe solution and the corresponding paper-based sensor with a sensitive limit of detection (LOD) of 34.5 nM. The present sensing strategy realizes the rapid visual and quantitative detection of CPs residues in food and the environment, and provides an effective way to utilization of organic reactions for trace hazardous substances detection.
研究领域:
分析化学
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环境科学
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食品安全
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有机化学
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传感器与执行器
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Ting Zhao,Xiaochen Liang,Xinjie Guo,Xingjie Yang,Jialiang Guo,Xia Zhou,Xueqin Huang,Wenqian Zhang,Yiqing Wang,Zhaowei Liu,Zhengjin Jiang,Hongke Zhou,Haibo Zhou
A simple, sensitive method for pesticide distinguishment based on a colorimetric sensor array using diverse gold nanoparticles (AuNPs) at room temperature is presented in this study. Acetylcholinesterase (AChE) hydrolysis ability was influenced by different pesticides and produced different concentrations of thiocholine by hydrolyzing acetylthiocholine iodide (ATCh). Thiocholine could be easily linked to the AuNPs through an Au − S covalent bond, and AuNPs underwent aggregation, resulting in a visible color change due to alteration of surface plasmon resonance properties. Based on these results, we successfully distinguished eight pesticides (glyphosate, thiram, imidacloprid, tribenuron methyl, nicosulfuron, thifensulfuron methyl, dichlorprop, and fenoprop) utilizing five different AuNPs by colorimetric assay. The limit of detection (LOD) of this visual method for all pesticides was less than 1.5 × 10 -7 M, which was more sensitive than the U.S. Environmental Protection Agency regulations specify (1.18 ∼ 3.91 × 10 -6 M). This method was further improved by combining a portable smartphone device with a color picking application using (color name AR) and RGB (red, green, blue) values. The method was successfully applied to pesticide residue distinguishment in real samples by linear discriminant analysis (LDA).
研究领域:
分析化学
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环境科学
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纳米技术
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食品安全
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jing Gu,Lu Zhang,Yangrui Si,Shuang Li,Jianzu Shen,Ruohan Xu,Tingxuan Yan,Mingfu Ye,Daojin Li,Qiang Wang,Changfang Zhang,Shuangshou Wang
Herein, an on-screen direct-reading solution was initiated for real-time monitoring of adsorption, degradation and recycle of dyes in water by integrating colorimetry with WeChat applet, in which MCM-41 composites were served as an all-rounder. Thanks to electrostatic interaction and mesoporous structures, MCM-41 showed superb binding performance toward dyes with the maximum extraction capacity up to 1.18 mg/mg (basic blue 26) and fast binding equilibrium within 1 min. Colorimetric cards with colors mapping to the dosage gradients of dyes were prepared by dyes-extracted MCM-41 or dye solutions, then RGB values of colorimetric cards were picked and embedded into a lab-made WeChat applet to build underlying digital colorimetric matrixes. Direct-reading analysis could be realized by automatic alignment of color information with inbuilt database of the applet in both online and offline ways with high sensitivity, in which the signal could still be readout even the dosage variations of dyes as small as 0.1 μg/mL. As proof-of-concept assays, this solution was finally applied to real-timely monitor the adsorption, degradation and recycle of dyes in water environment including industrial wastewater, aquaculture pond water and river water. Fine consistence between direct-reading results and those of colorimetry as well as instrumental technique like spectrometry assured high reliability of this strategy.
研究领域:
环境监测
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水处理
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分析化学
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纳米材料
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传感器
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信息技术
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污染控制
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产品关联
DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2020
·Vol. 167
·期 14
·147520
·
IF 3.721
Xinyi Wang,Liubing Kong,Shuqi Zhou,Tao Liang,Hao Wan,Ping Wang
Photoluminescence (PL) sensors based on quantum dots (QDs) are difficult to achieve exact detection in sample waters. In this paper, polyvinyl alcohol (PVA) based CdTe/ZnS/CdS QDs and fluorescein isothiocyanate (FITC) fluorophores were used to build a ratiometric PL sensor. Combining with the homemade smartphone-based PL E-eye, the Cd2+ exact detection can be achieved. PVA was used to connect QDs and FITC fluorophores without unnecessary ligand exchange and purification. For QDs fluorophore, ethylenediaminetetraaceticacid (EDTA) was used on the surface to induce the specifically Cd2+ recognition site. For FITC fluorophore, the PL remains unchanged in this experiment. Thus, the ratio of two fluorophores can be used to provide a built-in correction. The PL changes with the increase of Cd2+ concentrations could be displayed as a visual color change on the smartphone. Further quantitative analysis could be carried out by the RGB value of the picture through the App in less than 1 min The ratio of R/G is linear to Cd2+ concentration in the range of 1–2000 μg l−1 with a low LOD of 0.057 μg l−1 (S/N). Compared with traditional analysis methods, the PL ratiometric method with PL E-eye is portable, rapid, visible and highly selective especially in discriminate Cd2+ from Zn2+.
研究领域:
环境科学
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分析化学
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纳米技术
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光学传感
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DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Rui Li,Haoyu Feng,Shancan Wang,Di Zhuang,Youhong Wen,Jie Zhu
A double-layer film was developed with tannic acid (TA) co-pigmented purple potato anthocyanin extract (PAE)-agar as the inner layer, and K-carrageenan-oregano essential oil Pickering emulsion (OPE)/silver nanoparticles (TA-AgNPs) as the outer layer. Molecular docking and FT-IR results elucidated that intermolecular hydrogen bond was the main interaction between components in the agar-carrageenan matrix, with TA and PAE contributing to intensified anthocyanin color through π-π stacking. The incorporation of OPE/TA-AgNPs enhanced the film's hydrophobicity (WCA > 100 ° ) and UV–vis barrier (close to 0% at 200-320 nm, effectively impeding UVA, UVB, and UVC) properties and exhibited outstanding antioxidant (DPPH scavenging rate > 88%) and antimicrobial activities. This film showed a significant color change in the pH range of 2–12 (from pink to yellow) and a considerable sensitivity to volatile amines within 2 min. The films effectively alleviated beef spoilage (extending the shelf life of beef for 1d) and reflected the freshness of beef during storage. Additionally, the digital color information of the film was obtained by a smartphone combined with RGB values analysis to quantify the freshness of beef rapidly. Therefore, this study expands the application of food packaging films with freshness preservation and monitoring in the field of animal-derived food.
研究领域:
食品科学
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材料科学
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生物技术
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包装工程
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Qian Zhao,Xinhui Xie,Liye Chen,Jiahao Wu,Ziping Jiang,Xingbo Shi
Aflatoxin B1 (AFB1), one of the most toxic mycotoxins, is commonly found in various food. This study develops a dual-mode biosensor integrating smartphone analysis and dark-field microscopy (DFM) observation for AFB1 detection based on the disintegration of core-shell Ag@Au nanoparticles (Ag@Au NPs). Employing a sandwich assay format, AFB1 aptamer assembled Ag@Au NPs are anchored on 96-well plate and disintegrated by nitric acid. Thereafter, the Ag core is etched, releasing Ag + that serve as colorimetric signal regulators upon the introduction of the glutathione-manganese dioxide nanosheets-3,3′,5,5′-tetramethylbenzidine substrate. The color signal is analyzed using a smartphone based on red-green-blue value analysis. Furthermore, the collapsed Au shell releases individual Au NPs that serve as dark-field scattering nanoprobes, producing a high-intensity green dark-field scattering signal under DFM observation. The detection limit (3.67 fg/mL) is 199/8174/2180-fold lower than those of colorimetric signal, commercial enzyme-linked immunosorbent assay kit, and high-performance liquid chromatography, respectively. Overall, smartphone analysis enables on-site preliminary screening without sophisticated instrumentation. DFM observation allows the ultra-sensitive quantitative detection in a laboratory. The proposed dual-mode biosensor will not only reduce false-positive/negative rate through the bidirectional signal readout, but also offer a practical, stable, and ultrasensitive strategy for AFB1 detection in food.
研究领域:
食品科学
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分析化学
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纳米技术
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生物技术
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显微镜技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）