SENSORS AND ACTUATORS B-CHEMICAL
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2024
·
IF 8
Gongle Shen,Linling Zhong,Yuanhang Bi,Yifan Liu,Jiangqi Zhao,Xin Wen,Yifei Zhu,Lei Feng,Li Geng,Fan Yu,Changjun Hou,Xianfeng Wang
Considering the vital role of sulfide ions (S 2- ) in food safety, environmental pollution and public health, accurate and efficient detection of S 2- is of great importance. In this study, Er-MOFs with the structure of four-pointed star was successfully designed and synthesized using Er(III) as the metal center and TCPP as the ligand. It is found that host-guest effects occur between Er-MOFs and S 2- , blocking the ligand-to-metal charge transfer (LMCT) effect and O-H vibration. This progress leads to the fluorescence signal-on of Er-MOFs and the production of a brown color. In view of this, a Er-MOFs-based sensor was constructed for rapid and on-site monitoring of S 2- in water, which shows the triple-signal outputs including fluorescence, absorbance and smartphone-based RGB analysis with high sensitivity and selectivity. More importantly, the successful measurement of S 2- in real water samples with satisfactory recoveries from 96.26 % to 104.03 %, demonstrating the practicality and effectiveness of Er-MOFs for S 2- recognition. Overall, this assay exhibits the promising applications for S 2- measurement in the fields of environmental pollution and food safety.
研究领域:
材料科学
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环境科学
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食品安全
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Jing Feng,Sheng Yao,Xiaohan Mei,Jiacheng Xie,Xujie Wang,Huitian Zhi,Ruhui Shang,Yingchao Yang,Lei Hu,Zhengquan Yan
To conveniently monitor and remove toxic Hg 2+ and S 2− in food samples, Au Ag quantum-dot nanozyme (Au-Ag@FA QDs) was identified. The strong synergistic effect among folic acid (FA), Ag and Au endowed Au-Ag@FA QDs with superior peroxidase-mimic activity to accelerate 3,3′,5,5′-tetramethylbenzidine (TMB) redox with K m / V max of 0.28 mmol·L −1 /6.40 × 10 −8 mol·L −1 ·s −1 . Trace Hg 2+ or S 2− could selectively alter its peroxidase-like activity and inherent fluorescence performance, with obvious hyperchromic/“turn-on” fluorescent effect for Hg 2+ or hypochromic/“turn-off” one for S 2− . And red-green-blue (RGB) values analyzed from smartphones changed regularly with c Hg 2+ or c S 2− . Under the optimized conditions, Au-Ag@FA QDs were successfully applied for multi-mode UV–vis, fluorescent, and RGB monitoring of Hg 2+ or S 2− in food samples. The detection limits were 7.38 × 10 −7 /5.74 × 10 −8 /4.93 × 10 −6 g·L −1 and 1.99 × 10 −6 /4.48 × 10 −8 /5.79 × 10 −6 g·L −1 respectively. Importantly, over 93.8 % Hg 2+ or 90.5 % S 2− could be removed by simple centrifugal-separation after detection. The selective recognition mechanisms to Hg 2+ and S 2− were also proposed.
研究领域:
生物化学
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食品安全
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2020
·Vol. 13
·期 6
·809-816
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IF 2.596
Rui Li,Yanan Sun,Lihua Jin,Xiaohong Qiao,Cong Li,Yehua Shen
With the rapid development of point-of-care (POC) technologies, development of sensitive method featured with fast analysis and affordable devices has become an emerging requirement for practical applications. In this study, we introduced a smartphone-based RGB analysis system for the sensitive detection of acid phosphatase (ACP) enzyme activity. In the presence of ACP, L-ascorbic acid 2-phosphate (AAP) can be converted into ascorbic acid (AA), which can reduce Ag+ to Ag0 and format the Au@Ag core–shell nanostructure. This morphology change of the Au@Ag core–shell would trigger a significant color variation (pink to yellow). A good linear relationship between the RGB model parameter and the concentration of ACP could be obtained with a detection limit of 0.1 U L−1. Moreover, this sensing strategy is suitable for the detection of ACP in practical serum samples. Thus, this simple but powerful protocol has great potential application for on-site detection of ACP in future complex biological samples.
研究领域:
生物医学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 36
·3598-3604
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IF 3.532
Wenxiu Huang,Lizhen Zhao,Ruidi Shen,Gongke Li,Liansheng Ling
A highly sensitive and selective RGB color analysis for the detection of formaldehyde (FA) was developed by using a DNA functionalized gold nanoparticle (AuNPs-DNA) probe. When complementary oligonucleotides (oligo 2 and oligo 3) and a silver ion (Ag+) were added to the AuNPs-DNA solution, triplex DNA was formed, resulting in the aggregation of AuNPs, and accompanied by a solution color change from red to purple. With the addition of formaldehyde, it reacted with Ag+, decreased the stability of triplex DNA between AuNPs–DNA, induced the dispersion of AuNPs, and the color of AuNPs recovered to red. Therefore, the formaldehyde concentration could be estimated with the RGB (red, green, blue) values of the AuNP solution by using a smartphone application (APP). The R value of the system was proportional to the concentration of formaldehyde within the range of 0.23–4.50 mg L−1, with a detection limit of 0.14 mg L−1. The method has been successfully applied to detect the residues of formaldehyde in vegetable samples and has the potential of the on-site determination of formaldehyde.
研究领域:
生物化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
Journal of Pharmaceutical Analysis
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2024
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IF 6.1
Yue Liu,Taimei Cai,Sen Chen,Tao Wen,Hailong Peng
The overuse of antibiotics has led to the severe contamination of water bodies, posing a considerable hazard to human health. Therefore, the development of an accurate and rapid point-of-care testing (POCT) platform for the quantitative detection of antibiotics is necessary. In this study, Cerium oxide (CeO 2 ) and Ferrosoferric oxide (Fe 3 O 4 ) nanoparticles were simultaneously encapsulated into N-doped nanofibrous carbon microspheres to form of a novel nanozyme (CeFe-NCMzyme) with a porous structure, high surface area, and N-doped carbon material properties, leading to a considerable enhancement of the peroxidase (POD)-like activity compared with that of the CeO 2 or Fe 3 O 4 nanoparticles alone. The POD-like activity of CeFe-NCMzyme can be quenched using L-Cysteine (Cys) and subsequently restored by the addition of a quinolone antibiotic (norfloxacin, NOR). Therefore, CeFe-NCMzyme was used as a colorimetric sensor to detect NOR via an “On-Off” model of POD-like activity. The sensor possessed a wide linear range of 0.05-20.0 μM ( R 2 = 0.9910) with a detection limit of 35.70 nM. Furthermore, a smartphone-assisted POCT platform with CeFe-NCMzyme was fabricated for quantitative detection of NOR based on RGB analysis. With the use of the POCT platform, a linear range of 0.1-20.0 μM and a detection limit of 54.10 nM were obtained. The spiked recoveries in the water samples were ranged from 97.73% to 102.01%, and the sensor exhibited good accuracy and acceptable reliability. This study provides a portable POCT platform for the on-site and quantitative monitoring of quinolone antibiotics in real samples, particularly in resource-constrained settings.
研究领域:
环境科学
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分析化学
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生物技术
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即时诊断
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2025
·
IF 4.2
Haiyan Ma,Yinji Cui,He Gao,Ling Yan,Lan Liu,Longbin Xu
Hypochlorous acid (HClO), a key reactive oxygen species (ROS), plays a vital role in immune defense but is also implicated in the pathogenesis of inflammatory diseases. Excessive levels of HClO can cause environmental hazards by generating toxic chlorinated by-products in aquatic systems, compromising water quality and threatening both aquatic ecosystems and drinking water safety. In this study, we introduce a phenothiazine-based fluorescent probe (PHBF) engineered for rapid and selective detection of HClO. The probe exhibits an instantaneous fluorescence “turn-on” response within 5 seconds, with a detection limit of 48.54 nmol·L -1 , demonstrating its high sensitivity for real-time monitoring. For environmental applications, PHBF was integrated into an agarose gel matrix, resu-1ting in a portable gel-based sensing platform. This system enables on-site, real-time detection of HClO in water samples, utilizing smartphone-assisted RGB analysis for dual-mode fluorescence and colorimetric quantification. The RGB-based readout provides a cost-effective, user-friendly, and quantitative solution for environmental monitoring. Furthermore, the probe’s ability to image HClO in living cells confirms its biocompatibility and potential for biomedical applications. Overall, this study presents a multifunctional detection strategy for HClO, combining rapid fluorescence response, portable gel-based sensing, and smartphone-enabled RGB analysis. This approach offers a promising tool for real-time water quality assessment and biomedical research on oxidative stress.
研究领域:
分析化学
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环境科学
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生物化学
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材料科学
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生物医学工程
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产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2023
·
IF 9.9
Min Jia,Fupei Xu,Fei Zhai,Xiaoying Yu,Meixia Du
BPA aptamers functionalized cerium oxide nanoparticles encapsulated in zeolitic imidazolate framework-8 (CeO 2 @ZIF-8/Apt) were developed to fabricate an all-in-one portable platform for on-site quantitative detection of BPA. By combining biocomposites with a 3,3′,5,5′-tetramethylbenzidine (TMB)-based sodium alginate (SA) hydrogel and smartphone-based RGB analysis, highly sensitive and convenient monitoring of BPA was achieved. CeO 2 @ZIF-8 composites were constructed using a novel surfactant-modified concentration-controlled synthesis strategy. After being functionalized with BPA aptamers, CeO 2 @ZIF-8/Apt biocomposites were used as target-response colorimetric probes for target recognition and signal transduction. The oxidase-like activity of CeO 2 @ZIF-8 was effectively sealed by BPA aptamers and controllably released in a concentration-dependent manner through aptamer-BPA reactions. Utilizing SA hydrogels containing TMB in the caps, a one-step sample addition and one-pot detection can be conveniently achieved and reliably quantified by smartphone-based RGB analysis in an instrument-free way. The detection range of this portable detection platform is 50 pg/mL to 500 ng/mL with limit of detection calculated as 34.88 pg/mL, comparable to that of conventional detection in the solution system (4.57 pg/mL). The recoveries in tap water, apple juice, and milk ranged from 91.02 % and 106.75 %. This work contributes new insights into the design of all-in-one detection platforms for contaminants monitoring in resource-constrained regions.
研究领域:
环境科学
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分析化学
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纳米技术
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生物技术
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Xianghong Xie,Bangfeng Fu,Mingyu Zhang,Wentao Zhang,Zhenghua Xu,Shuwen Liu,Jianlong Wang
The implementation plan of extending the shelf life of food while ensuring food safety is playing an increasingly important role in promoting global solutions to food waste. Here, we have logically created a new eugenol nanoemulsion to sense spoilage and extend the shelf life. The nano lotion (Eu/SA) was loaded with sodium alginate nanoparticles as carrier, eugenol as antibacterial agent and antioxidant. Fluorescein isothiocyanate (FITC) was utilized concurrently as an indicator by simply mixed with Eu/SA nanoemulsion, then physically deposited on a commercial filter membrane to create smart fluorescent labels. Shrimp with and without the Eu/SA nanoemulsion coating had their freshness monitored using a non-contact tagging technique. And through smartphone integrated with RGB (red/green/blue) analysis for digitization, it was found that the RGB of uncoated shrimp was greater than that of treated shrimp. Additionally, without coating treatment, thiobarbituric acid (TBA), pH and viable bacterial count (TVC) of Shrimp all exceed the established. Eu/SA nanoemulsion offers a more appealing monitoring and preservation integration platform for the food safety industry.
研究领域:
食品科学
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纳米技术
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生物技术
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化学工程
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传感器与执行器
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ying Huang,Xiangrong Liu,Tiantian Shen,Wang Li,Jiali Ren,Haiyan Zhong
The detection techniques for glyphosate residues have garnered attention due to the potential toxicity associated. Herein, we introduced a fluorescent “turn-on” strategy, leveraging competitive coordination with iron, for glyphosate detection. We synthesized iron-based metal-organic frameworks (Fe-MOFs) at room temperature. Among them, 2,5-dihydroxyterephthalic acid (DOBDC) acted as a ligand to produce strong fluorescence, and iron ions acted as a quencher to quench the fluorescence of DOBDC. Another ligand, 2-nitroterephthalic acid, contained nitro quenching of the fluorescence, giving it a lower background. Glyphosate competed with the ligands for ions, weakening the interaction between ions and the ligands. This disruption restored the fluorescence emission at 535 nm, enabling glyphosate detection. To facilitate field application, Fe-MOFs were encapsulated within agarose to create a functional hydrogel. Glyphosate penetrated the hydrogel and resulting in fluorescence “turn-on”. Remarkably, this method facilitated visual detection through smartphone photography coupled with RGB (red/green/blue) analysis, offering a user-friendly and portable solution.
研究领域:
环境科学
·
分析化学
·
材料科学
·
传感器和检测技术
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产品关联
DOI
PubMed
高影响因子
Advanced Optical Materials
·
2023
·Vol. 11
·期 14
·2300010
·
IF 9
Yuanhua Zhang,Yan Qiu,Lan Xia,Long Yuan,Hailong Wu,Rui Shi,Anqi Chen,Kai Zhou,Mingxue Zhou,Kailian Zhang,Chunxiang Zhang,Zhining Xia,Qifeng Fu
The emerging visualization techniques have aroused wide interest for optical information detection and storage. However, they suffer from fairly limited optical information density and poor visual detection performance. Herein, an innovative surface patterning enhanced visualization (SPEV) approach is developed for ultrasensitive naked-eye detection and RGB-based high-density information storage. Through “ship covalently linked in bottle” method, a novel HP-UiO-66-NH 2 -based ratiometric fluorescent (RF) composite probe is fabricated, exhibiting remarkably enhanced CdTe QDs encapsulation efficiency and superior dual-sensing performance for F − and alkaline phosphatase (ALP). Then, the novel surface patterned CdTe QDs@HP-UiO-66-NH 2 hydrogel sensor is prepared, emitting multi-stimuli-responsive optical information with significantly improved abundance and dimensionality. The pseudo-color postprocessing of the fluorescence images is performed to convert the analyte-induced imperceptible color variations into easily perceptible color tonality change, resulting in ultra-high sensitivity and excellent accuracy for naked-eye quantitative detection of ALP and F − in human serum. Furthermore, the highly accurate pattern block-based RGB (PB-RGB) assay is demonstrated and the concept of RGB-based 3D patterning codes is presented for highly reliable extraction of quantitative color information and high-density information storage. The SPEV technique provides a universal route to realize instrument-free precise visual quantitative detection, high-density optical information storage, multi-stimuli-responsive encryption, and anti-counterfeiting.
研究领域:
光学传感
·
材料科学
·
纳米技术
·
生物医学工程
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信息存储
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化学传感器
·
荧光成像
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）