Journal of Materials Chemistry B
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2025
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IF 5.7
XIAOXIAO YUAN,Xiaoyi Zhang,YIFAN YANG,YAONAN WANG,SHURUI ZHAO,QIQI FENG,Jianhui Wu,MING ZHAO,Shiqi Peng
Here a novel anti-osteoporosis agent UA-RGDS, namely RGDS (Arg-Gly-Asp-Ser) conjugated UA (ursolic acid), was presented. In ultrapure water UA-RGDS existed as the tetramer. In the serum of OVX mice (ovariectomized mice), and at 10-4 M of concentration the self-assemble led UA-RGDS to the formation of nano-particles of 16 nm-156 nm in height, the size of suitably transporting in the blood circulation. In vivo bio-distribution supported that UA-RGDS only distributed in the femur of OVX mice, while null distribution been found in the blood and the other organs. These observations suggested that UA-RGDS was capable of targeting the femur, thereby blocked bone loss. ELISA experiment explored that as anti-osteoporosis agent UA-RGDS down regulated the expressions of IL-1β in the serum OVX mice. Thus UA-RGDS provided a linker for bone loss and down regulation of the expressions of IL-1β, thereby made serum IL-1β to be a biomarker for menopause women clinically.
研究领域:
药理学
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分子生物学
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纳米医学
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DOI
PubMed
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
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2016
·Vol. 18
·期 12
·1-16
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IF 2.101
Li Zhenzhen,Zhang Qiyi,Huang Huaying,Ren Changjing,Pan Yujin,Wang Qing,Zhao Qiang
In the experiments, high-quality, water-soluble and near-infrared (NIR)-emitting CdSeTe and CdSeTe/CdS quantum dots (QDs) were successfully prepared. The average size of CdSeTe⁄CdS QDs was 7.68 nm and CdSeTe QDs was 4.33 nm. Arginine-glycine-aspartic-serine acid (RGDS) peptides were linked to CdSeTe/CdS QDs by N-(3-(dimethylamino)propyl)-N′-ehtylcarbodiimide hydrochloride (EDC) and N′-hydroxysuccinimide (NHS). The prepared RGDS-tagged NIR CdSeTe/CdS QDs (denoted as RGDS-CdSeTe/CdS) had an average diameter of 24.83 nm and were used for cancer cell immunofluorescence imaging. The characteristics of RGDS-conjugated CdSeTe/CdS such as morphology, structure, spectra, stability, cytotoxicity, and near-infrared microscopic imaging were investigated in detail. HepG2 cells were incubated with the novel fluorescent probe (RGDS-CdSeTe/CdS), which realized immunofluorescence targeting and imaging. The results reported here open up new perspectives for integrin-targeted near-infrared imaging and may aid in tumor detection including imaging-guided surgery.
研究领域:
生物医学工程
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纳米医学
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肿瘤学
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DOI
高影响因子
ELECTROCHIMICA ACTA
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2014
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IF 4.086
Xiaobo Wang,Jing Ju,Juan Li,Jiying Li,Qiuhui Qian,Chun Mao,Jian Shen
It is highly desirable to develop simple and rapid method to detect cancer cells. In this case, a simple method for electrochemical detecting cell numbers based cytosensor was investigated. Thioctic acid (TA) monolayer, assembled on a high conductive Au/ITO substrate to stable immobilized of Arg-Gly-Asp-Ser tetrapeptide (RGDS) by chemical bonding, was first used for construction of electrochemical cytosensor. Moreover the cytotoxicity assay also showed no cytotoxicity of TA modified electrode . The proposed cytosensor showed good performance for detection of cancer cells with a broad detection range of 3.0 × 10 2 to 1.0 × 10 7 cells/mL, and a low detection limit of 300 cells/mL. The results were obtained that can be attributed to the combination of the advantages of high conductive and biocompatible property of the modified electrode we suggested. This work demonstrated a simple strategy to prepare an electrochemical cytosensor with the property of low cost, good biocompatible, broad detection range, low detection limit, good repeatability which may have potential large-scale application for improving cancer diagnosis and treatment.
研究领域:
生物医学工程
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癌症诊断
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DOI
高影响因子
Biomolecules
·
2023
·Vol. 13
·期 5
·793
·
IF 5.5
Haiyan Liu,Yang Hu,Xiuping Wu,Rong Hu,Yingyu Liu
Bacterial cellulose (BC) has been widely used in tissue engineering due to its unique spatial structure and suitable biological properties. In this study, a small biologically active Arginine-Glycine-Aspartic acid-Serine (RGDS) tetrapeptide was incorporated on the porous BC surface followed by a low-energy CO2laser etching operation. As a result, different micropatterns were established on the BC surface with RGDS only anchored on the raised platform surface of the micropatterned BC (MPBC). Material characterization showed that all micropatterned structures exhibited platforms with a width of ~150 μm and grooves with a width of ~100 μm and a depth of ~300 μm, which displayed distinct hydrophilic and hydrophobic properties. The resulting RGDS-MPBC could hold the material integrity, as well as the microstructure morphology under a humid environment. In-vitro and in-vivo assays on cell migration, collagen deposition, and histological analysis revealed that micropatterns led to significant impacts on wound healing progress compared to the BC without surface-engineered micropatterns. Specifically, the basket-woven micropattern etched on the BC surface exhibited the optimal wound healing outcome with the presence of fewer macrophages and the least scar formation. This study further addresses the potential of adopting surface micropatterning strategies to promote skin wounds towards scar-free outcomes.Keywords:bacterial cellulose;lithography;micropatterns optimization;scar-free
研究领域:
组织工程
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生物医学工程
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材料科学
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DOI
PubMed
高影响因子
International Journal of Nanomedicine
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2024
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IF 6.6
Zixia Wu,Weibo Xia,Liling Ou,Ling Zheng,Bingying Hou,Tonghe Pan,Wenjie Sun,Leo H Koole,Yongqing Shao,Lei Qi
Purpose Patients afflicted with dry eye disease (DED) experience significant discomfort. The underlying cause of DED is the excessive accumulation of ROS on the ocular surface. Here, we investigated the nitrogen doped-graphene quantum dots (NGQDs), known for their ROS-scavenging capabilities, as a treatment for DED.Methods NGQDs were prepared by using citric acid and urea as precursors through hydrothermal method. The antioxidant abilities of NGQDs were evaluated through: scavenging the ROS both extracellular and intracellular, regulating the nuclear factor-erythroid 2-related factor (Nrf2) antioxidant pathway of human corneal epithelial cells (HCECs) and their transcription of inflammation related genes. Furthermore, NGQDs were modified by Arg-Gly-Asp-Ser (RGDS) peptides to obtain RGDS@NGQDs. In vivo, both the NGQDs and RGDS@NGQDs were suspended in 0.1% Pluronic F127 (w/v) and delivered as eye drops in the scopolamine hydrobromide-induced DED mouse model. Preclinical efficacy was compared to the healthy and DPBS treated DED mice.Results These NGQDs demonstrated pronounced antioxidant properties, efficiently neutralizing free radicals and activating the intracellular Nrf2 pathway. In vitro studies revealed that treatment of H2O2-exposed HCECs with NGQDs induced a preservation in cell viability. Additionally, there was a reduction in the transcription of inflammation-associated genes. To prolong the corneal residence time of NGQDs, they were further modified with RGDS peptides and suspended in 0.1% Pluronic F127 (w/v) to create RGDS@NGQDs F127 eye drops. RGDS@NGQDs exhibited superior intracellular antioxidant activity even at low concentrations (10 μg/mL). Subsequent in vivo studies revealed that RGDS@NGQDs F127 eye drops notably mitigated the symptoms of DED mouse model, primarily by reducing ocular ROS levels.Conclusion Our findings underscore the enhanced antioxidant benefits achieved by modifying GQDs through nitrogen doping and RGDS peptide tethering. Importantly, in a mouse model, our novel eye drops formulation effectively ameliorated DED symptoms, thereby representing a novel therapeutic pathway for DED management.
研究领域:
眼科
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材料科学
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药学
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细胞生物学
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DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
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2019
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IF 4.959
Yang Hu,Haiyan Liu,Xin Zhou,Haobo Pan,Xiuping Wu,Noureddine Abidi,Yongjun Zhu,Jinhui Wang
Bacterial cellulose (BC) is a bio-derived polymer, and it has been considered as an excellent candidate material for tissue engineering. In this study, a crossed groove/column micropattern was constructed on spongy, porous BC using low-energy CO 2 laser photolithography. Applying the targeted immobilization of a tetrapeptide consisting of Arginine-Glycine-Aspartic acid-Serine (H-Arg-Gly-Asp-Ser-OH, RGDS) as a fibronectin onto the column platform surface, the resulting micropatterned BC (RGDS-MPBC) exhibited dual affinities to fibroblasts and collagen. Material characterization of RGDS-MPBC revealed that the micropattern was built by the column part with size of ~100 × 100 μm wide and ~100 μm deep, and the groove part with size of ~150 μm wide. Hydrating the MPBC did not result in the collapse of the integrity of the micropattern, suggesting its potential application in a highly hydrated wound environment. Cell culture assays revealed that the RGDS-MPBC exhibited an improved cytotoxicity to mouse fibroblasts L929, as compared to the pristine BC. Meanwhile, it was observed that the RGDS-MPBC was able to guide the ordered aggregation of human skin fibroblast (HSF) cells on the column platform surface, and no HSF cells were found in the groove channels. Over time, it was found that a dense network of collagen was gradually established across the groove channels. Furthermore, the in-vivo animal study preliminarily demonstrated the scar-free healing potential of the micropatterned BC materials. Therefore, this RGDS-MPBC material exhibited its advantages in guiding cell migration and collagen distribution, which could present a prospect in the establishment of “basket-woven” organization of collagen in normal skin tissue against the formation of dense, parallel aggregation of collagen fibers in scar tissue toward scar-free wound healing outcome.
研究领域:
组织工程
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生物医用材料
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伤口愈合
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2021
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IF 9.229
Yuanqing Zhu,Rong Fan,Zhiyuan Zheng,Zhiqiang Zhu,Ting Si,Ronald X. Xu
We propose a compound interfacial shearing (CIS) process for versatile production of monodisperse Janus emulsions with controllable structural and topographic features. The process induces an active periodic force to decouple material and process parameters, enables independent control of compartmental features in Janus emulsions, and facilitates inline and on-demand generation of various geometric features for a large variety of process parameters and material properties. Janus emulsions of poly(ethylene glycol) diacrylate (PEGDA) with a controlled number of compartments are produced by CIS and photopolymerized to form micro-hydrogels with designated interfacial curvatures. PEGDA micro-hydrogels can be further modified to achieve anisotropy of surface or internal features by the content of an oily dispersed phase. MCF-7 human breast cancer cells are encapsulated in micro-hydrogels for cell proliferation with satisfactory viability. By modifying PEGDA micro-hydrogels with RGDS-conjugated polystyrene microspheres, we have demonstrated the controlled spatial adhesion of MCF-7 cells and human umbilical vein endothelial cells (HUVECs) on the substrates of different three-dimensional (3D) curvatures. Our pilot study suggests a simple and potentially scalable approach to produce 3D substrates with controllable structural and topographic features for 3D guided cell organization.
研究领域:
材料科学
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生物技术
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细胞生物学
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组织工程
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产品关联
DOI
PubMed
高影响因子
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