ENVIRONMENTAL POLLUTION
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2019
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IF 5.714
Wanling Cai,Xiulan Weng,Zuliang Chen
Antibiotics in water and soil are persistent, bioaccumulative and toxic to aquatic organisms and human health. To address it, as one of the new technologies , green synthesized magnetic Fe 3 O 4 nanoparticles by Excoecaria cochinchinensis extract used to remove rifampicin (RIF) was investigated in this study. Results showed the adsorption efficiency of RIF reached 98.4% and the maximum adsorption capacity is 84.8 mg/g when 20 mL of RIF at a concentration of 20 M was adsorbed by 10 mg Fe 3 O 4 at a temperature of 303 K. The morphology of the green Fe 3 O 4 characterized by SEM demonstrated the dimensions ranging from 20 to 30 nm. The N 2 adsorption/desorption isotherms revealed that the surface area of Fe 3 O 4 was 111.8 m 2 /g. In addition, adsorption studies indicated that the kinetics fitted the pseudo second-order and isothermal adsorption conformed to the Langmuir isotherm. Furthermore, due to their magnetic properties, the Fe 3 O 4 nanoparticles were easily separated and reused and the mechanism for removing RIF occurred through adsorption rather than chemical redox reaction. Finally, the reusability of Fe 3 O 4 for adsorption of RIF showed that the removal efficiency decreased to 61.5% after five cycles.
研究领域:
环境科学
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材料科学
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生物学
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DOI
PubMed
高影响因子
Applied Biological Chemistry
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2024
·Vol. 67
·期 1
·1-11
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IF 2.3
Yu Ziqing,He Wen,Shi Weiwu
All chronic renal disorders eventually lead to renal interstitial fibrosis (RIF). Chronic inflammation and pro-fibrotic substances are familiar companions of the fibrotic process. The Sulforaphane (Sul) molecule is particularly useful in protecting the liver from oxidative damage. To investigate the Sul effects on fibrosis markers and inflammatory proteins in the kidney of NRK52E cell line and rats and clarify the mechanism of TGF-β/Smad signaling pathway in a rat model of RIF were developed in the present study. Sul (50, 100, and 200 ng/ml) remarkably reduced the gene expressions of tumor necrosis factor (TNF-α), interleukin-6 (IL-6), interleukin (IL)-1β, collagen 3 (COL3A1), collagen 1 (COL1A1), and α-smooth muscle actin (α-SMA) in fibrotic NRK52E cells compared with those in cells inspired by transforming growth factor-α (TGF-α). Histopathological investigations showed that Sul administration retained renal tissue structure and decreased kidney tissue fibrosis in rats subjected to unilateral ureteral blockage (UUO). The expression level of TNF-α, IL-6, IL-1β, COL3A1, COL1A1, and α-SMA in the rats’ kidneys exposed to UUO was also suppressed by the treatment of Sul. In the present study, western blot analysis showed that Sul upregulated the expressions of fibrotic NRK52E cells Smad7 and rat model UUO groups while simultaneously decreasing the stimulation of Smad2/3 and the expressions of cyclooxygenase-2, NF-κB, Smad4, activator protein-1, and high-mobility group protein B1. Ultimately, Sul’s ability to inhibit the TGF-β/Smad pathway and the development of inflammation factors may mitigate RIF.
研究领域:
医学
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药理学
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分子生物学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Shumei Zhang,Shaoyun Shan,Heng Zhang,Xin Gao,Xiaoning Tang,Keli Chen
Wound healing is a challenged and complicated process due to the bacterial infections and frequent replacement in healing process. Hydrogels with properties of visibility and biocompatibility provided convenient and effective treatment during the wound healing process. Bamboo parenchyma cells have a great potential utilized on cellulosic materials fabrication for their high specific surface area and accessibility of chemical reagents. Herein, we present a simple and facile manufacture of transparent wound dressing from bamboo parenchymal cellulose via dissolution in DMAc/LiCl system. Rifampicin (RIF) was loaded on the hydrogel through immersion method. The result exhibited that the maximum drug loading efficiency of cellulose hydrogels was 82.13%. Hydrogel loaded RIF (HLR) showed that the inhibition zones against Gram-negative and Gram-positive bacteria were 19.11 mm and 36.93 mm, respectively. It was observed that the wound was healed more than 60% at 11th day in murine wound models. Meanwhile, RIF provided an exceptionally antibacterial property to hydrogels and promoted proliferation of epidermis cells in wound. As a result of observations, HLR demonstrating potential application in visual wound dressing materials for their excellent transparency, antibacterial effect, wound healing, and biocompatibility.
研究领域:
生物医学工程
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材料科学
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药学
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DOI
PubMed
高影响因子
Frontiers in Pharmacology
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2025
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IF 4.8
Donghan Bai,Zihao Zhang,Jingyue Gao,Qiaochu Wang,Remy Macdonald,Ziwen Xu,Shumin Chen,Nanxi Huang,Lu Luo
Background: Leukemia is a malignant hematologic disease that poses a serious threat to human health. Realgar-Indigo Naturalis formula (RIF), a traditional Chinese medicine formula, have demonstrated anti-tumor activity, but its mechanisms of action remain unclear.Objective: This study aimed to establish a zebrafish HL-60 tumor transplantation model to investigate the anti-leukemic effects of RIF and explore its underlying mechanisms.Methods: The zebrafish HL-60 tumor transplantation model was established, with RIF as the intervention drug and all-trans retinoic acid (ATRA) as the control. Anti-tumor efficacy was assessed via pharmacodynamic analysis. Transcriptomic analysis further elucidated gene expression profiles, differentially expressed genes, and relevant biological pathways.Results: RIF significantly reduced tumor cell fluorescence intensity, demonstrating anti-tumor efficacy. Additionally, it improved HL-60-induced liver damage in zebrafish, as evidenced by a reduction in fatty vacuolar degeneration in liver tissue. Transcriptomic analysis revealed that ATRA mainly affected the FoxO signaling pathway, PI3K-Akt signaling pathway, apoptosis, and complement and coagulation cascades in leukemia treatment. RIF primarily influenced the ubiquitin-proteasome system, ferroptosis, and glutathione metabolism. The combination of ATRA and RIF also affected autophagosome and lysosome pathways, in addition to the aforementioned pathways.Conclusion: RIF exhibit significant anti-tumor effects through modulation of multiple pathways. This study provides a theoretical foundation for the potential clinical application of RIF in leukemia treatment.
研究领域:
肿瘤学
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药理学
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中医学
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发育生物学
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毒理学
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基因组学
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DOI
PubMed
高影响因子
DENTAL MATERIALS
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2025
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IF 6.3
Yan Wang,Li Liu,Liyuan Zhou,Huan Cheng,Yifan Li,Anni Wu,Senjeet Sreekissoon,Xiao Chen,Peng Gao,Lei Lu,Zhennan Deng,Jinsong Liu
Postoperative infection significantly increases the risk of failure in titanium (Ti) dental implants. While the local application of antimicrobial agents can effectively mitigate these infections. Electrospinning has gained considerable attention for its ability to produce high-performance nanofibers suitable for localized drug release. Nevertheless, challenges such as weak interfacial bond with Ti surface limits its application as a surface coating strategy. In this study, we developed a novel electrospun coating composed of a plant polyphenol, epigallocatechin gallate (EGCG)-modified polycaprolactone (PCL) and gelatin (Gel), which enhanced its adhesion to the Ti surface through phenol-amine chemistry. This innovative approach significantly enhanced the interfacial bond strength between the nanofibers and the Ti surface, thereby improving the wear resistance of the coating. Additionally, the incorporation of rifampicin (Rif), a broad-spectrum antibiotic, into the EGCG-modified electrospun coating (Ti/NFs-0.5 %EG@Rif) enabled sustained Rif release over 28 days, providing potential long-term antibacterial activity. Both in vitro and in vivo studies demonstrated that the Ti/NFs-0.5 %EG@Rif coating exhibited significantly enhanced antibacterial and anti-inflammatory efficacy, along with excellent biocompatibility. In conclusion, the EGCG-modified electrospun coating represented a promising and cost-effective strategy for enhancing the functionality of Ti implants, with the potential to improve clinical outcomes in dental applications.
研究领域:
生物材料
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纳米技术
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牙科
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药剂学
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表面工程
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
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