JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Tianyi Zhang,Changwei Gong,Jian Pu,Anchun Peng,Jizhi Yang,Xuegui Wang
Solenopsis invicta, an extremely destructive invasive species, has rapidly spread in China, with queens exhibiting chemical tolerance. In this study, bioassays were conducted on S. invicta colonies collected in Nanchong, revealing that the LC50 value of flonicamid for queens (3.91 mg/L) was significantly higher than that for workers (1.07 mg/L). Comparative analysis of transcriptomes of workers and queens treated with flonicamid revealed that differentially expressed genes (DEGs) were significantly enriched in the metabolism of xenobiotics by cytochrome P450 and drug metabolism by cytochrome P450 pathways. Based on the screening of transcriptome data, CYP6AQ83 might be involved in the detoxification metabolism of flonicamid in queens. After RNA interference, the sensitivity of queens to flonicamid was significantly increased by 30% in the treatment of the dsCYP6AQ83 group. Furthermore, heterologous overexpression of CYP6AQ83 in Drosophila melanogaster also significantly enhanced the tolerance against flonicamid. These results indicated that the overexpression of CYP6AQ83 in the queen enhances the tolerance against flonicamid.
研究领域:
昆虫学
·
分子生物学
·
害虫防治
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2014
·
IF 3.916
Jingfen Han,Jia Cai,Wuyinga Borjihan,Tsogzolmaa Ganbold,Tariq M. Rana,Huricha Baigude
RNA interference (RNAi) down-regulates gene expression post-transcriptionally, which is a therapeutically significant phenomenon that could potentially reduce the level of disease related proteins that are undruggable by conventional small molecular approaches. However, clinical application of small interference RNAs (siRNAs) requires design of potent siRNA sequences and development of safe and efficient delivery systems. To create a biocompatible siRNA delivery agent, we chemically modified natural polysaccharide curdlan in a regioselective manner to introduce amino group in the glucose units. The resulting 6-amino-curdlan (6AC) is water soluble and forms nanoparticles upon complexing with siRNAs. The novel curdlan-based nanoparticles efficiently delivered siRNAs to human cancer cells and mouse primary cells, and reduced 70–90% of target mRNA level. Moreover, 6AC nanoparticles delivered siRNA targeting eGFP to mouse embryonic stem (mES) cells stably expressing eGFP, and produced substantial reductions of GFP protein level. The novel curdlan-based nanoparticle is a promising vehicle for delivery of short RNAs to knock down endogenous mRNAs.
研究领域:
生物技术
·
分子生物学
·
药物递送
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CELLULAR PHYSIOLOGY
·
2021
·Vol. 236
·期 8
·5921-5936
·
IF 6.384
Sijia Liang,Jing Luo,Muna Alariqi,Zhongping Xu,Aoli Wang,Muhammad Naeem Zafar,Jun Ren,Fuqiu Wang,Xuefei Liu,Yanfeng Xin,Haonan Xu,Weifeng Guo,Yanqin Wang,Weihua Ma,Lizhen Chen,Keith Lindsey,Xianlong Zhang,Shuangxia Jin
Plant bugs (Miridae species) have become major agricultural pests that cause increasing and severe economic damage. Plant-mediated RNA interference (RNAi) is emerging as an eco-friendly, efficient, and reliable strategy for pest management. In this study, we isolated and characterized a lethal gene of Apolygus lucorum and named it Apolygus lucorum LIM ( AlLIM ), which produced A. lucorum mortality rates ranging from 38% to 81%. Downregulation of the AlLIM gene expression in A. lucorum by injection of a double-stranded RNA (dsRNA) led to muscle structural disorganization that resulted in metamorphosis deficiency and increased mortality. Then we constructed a plant expression vector that enabled transgenic cotton to highly and stably express dsRNA of AlLIM ( dsAlLIM ) by Agrobacterium -mediated genetic transformation. In the field bioassay, dsAlLIM transgenic cotton was protected from A. lucorum damage with high efficiency, with almost no detectable yield loss. Therefore, our study successfully provides a promising genetically modified strategy to overpower A. lucorum attack.
研究领域:
植物保护
·
分子生物学
·
农业科学
✓ 已验证
DOI
PubMed
高影响因子
Insect Science
·
2015
·Vol. 24
·期 4
·543-558
·
IF 2.144
Qin-Hui Zhang,Zhong-Nan Wu,Jing-Jiang Zhou,Yong-Jun Du
Olfaction is primarily mediated by highly specified olfactory receptors (ORs). Here, we cloned and identified an olfactory receptor, named SlituOR1 (Genbank no. JN835269), from Spodoptera litura and found evidence that it is a candidate pheromone receptor. It exhibited male-biased expression in the antennae, where it was localized at the base of sensilla trichoidea, the antennal sensilla mainly responsive to pheromones in moths. Conserved orthologues of this receptor, found among known pheromone receptors within the Lepidoptera, and SlituOR1 were placed among a clade of candidate pheromone receptors in a phylogeny tree of insect OR gene sequences. SlituOR1 showed differential expression in S. litura populations attracted to traps baited with different ratios of the two sex pheromone components (9 Z ,11 E )-tetradecadienyl acetate ( Z 9 E 11-14:OAc) and (9 Z ,12 E )-tetradecadienyl acetate ( Z 9 E 12-14:OAc). Knocking down of SlituOR1 by RNA interference reduced the electroantennogram (EAG) response to Z 9 E 11-14:OAc, and this result is consistent with the field trapping experiment. We infer that variation in transcription levels of olfactory receptors may modulate sex pheromone perception in male moths and could provide some of the flexibility required to maintain the functionality of communication with females when a population is adapting to a new niche and reproductive isolation becomes an advantage.
研究领域:
昆虫学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2023
·
IF 5.6
Shengnan Zhai,Hang Liu,Xianchun Xia,Haosheng Li,Xinyou Cao,Zhonghu He,Wujun Ma,Cheng Liu,Jianmin Song,Aifeng Liu,Jingjuan Zhang,Jianjun Liu
Polyphenol oxidase (PPO) activity is a major cause of the undesirable brown color of wheat-based products. Ppo1 , a major gene for PPO activity, was cloned based on sequence homology in previous studies; however, its function and regulation mechanism remain unclear. In this study, the function and genetic regulation of Ppo1 were analyzed using RNA interference (RNAi) and Targeting Induced Local Lesions IN Genomes (TILLING) technology, and superior mutants were identified. Compared with the control, the level of Ppo1 transcript in RNAi transgenic lines was drastically decreased by 15.5%–60.9% during grain development, and PPO activity was significantly reduced by 12.9%–20.4%, confirming the role of Ppo1 in PPO activity. Thirty-two Ppo1 mutants were identified in the ethyl methanesulfonate (EMS)-mutagenized population, including eight missense mutations, 16 synonymous mutations, and eight intron mutations. The expression of Ppo1 was reduced significantly by 6.7%–37.1% and 10.1%–54.4% in mutants M092141 (G311S) and M091098 (G299R), respectively, in which PPO activity was decreased by 29.7% and 28.8%, respectively, indicating that mutation sites of two mutants have important effects on PPO1 function. Sequence and structure analysis revealed that the two sites were highly conserved among 74 plant species, where the frequency of glycine was 94.6% and 100%, respectively, and adjacent to the entrance of the hydrophobic pocket of the active site. The M092141 and M091098 mutants can be used as important germplasms to develop wheat cultivars with low grain PPO activity. This study provided important insights into the molecular mechanism of Ppo1 and the genetic improvement of wheat PPO activity.
研究领域:
植物学
·
遗传学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
GENETICA
·
2024
·Vol. 153
·期 1
·1-11
·
IF 1.3
Gao Shanshan,Cao Yizhuo,Miao Wenbo,Li Dongyu,Zhou Can,Zhang Kunpeng
Eucalyptol is one of the major insecticidal active ingredients in a variety of plant essential oils, and has good killing and avoidance effects on Tribolium castaneum . The presence of detoxifying enzymes glutathione S-transferase (GST) in T. castaneum makes it resistant to a variety of insecticides. However, whether GST is involved in regulating the sensitivity of eucalyptol by T. castaneum is not well understood. In our previous study, a glutathione S-transferase, TcGSTu1 , was significantly up-regulated in RNA sequencing data when T. castaneum was exposed to eucalyptol. Therefore, in this study, the role of TcGSTu1 in the regulating the sensitivity of T. castaneum to eucalyptol was studied. The enzyme activities of GST and the transcription levels of TcGSTu1 were significantly increased following stimulation with eucalyptol. When using RNA interference technology knockdown TcGSTu1 heightens the sensitivity of T. castaneum to eucalyptol, demonstrating a link between TcGSTu1 and eucalyptol detoxification metabolism. Furthermore, TcGSTu1 is expressed in all developmental stages of T. castaneum , with higher expression levels observed particularly in the late egg stage. There was significant expression of TcGSTu1 in various tissues of different organisms, including larval head, fat body, and adult head. This observation indicated a possible connection between high TcGSTu1 expression and eucalyptol detoxification. The present findings suggest that TcGSTu1 may be involved in regulating the sensitivity and response of T. castaneum to treatment with eucalyptol, providing new research insight into pest control.
研究领域:
昆虫学
·
分子生物学
·
害虫防治
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2025
·
IF 4.5
Qiang Tang,Jiayi Zhang,Jialuo Pang,Yingying Huang,Ying Guan,Yuanxun Gong,Qianli Tang,Kai Zhang,Xianjiu Liao
This study introduces an innovative electrochemiluminescence (ECL) biosensor for the highly sensitive and specific detection of Argonaute 2 (Ago2) activity. Ago2, a key enzyme in the RNA interference (RNAi) pathway, plays a crucial role in gene regulation, and its dysregulation is associated with diseases such as cancer and viral infections. The biosensor integrates hybridization chain reaction (HCR) amplification and the CRISPR/Cas12a system, leveraging a multi-stage signal amplification strategy. The detection mechanism begins with Ago2-mediated cleavage of a designed hairpin RNA (HP-RNA), releasing single-stranded RNA (ssRNA) that triggers HCR. This amplification step generates long DNA polymers, which serve as activators for the CRISPR/Cas12a system. Cas12a's collateral cleavage activity amplifies the signal further by cleaving a DNA reporter labeled with a ruthenium-based luminophore, enhancing the ECL output. This dual amplification strategy achieves exceptional sensitivity, with a detection limit of 0.126 aM. The biosensor demonstrates excellent specificity, distinguishing Ago2 from other Argonaute proteins, and maintains high reproducibility and stability, retaining 94 % of its signal after two weeks of storage. Real-world applicability was confirmed by accurately detecting Ago2 in spiked cell lysates, with recovery rates exceeding 100 %. The combination of HCR, CRISPR/Cas12a, and ECL establishes a robust platform for biomarker detection, offering superior sensitivity and adaptability for clinical diagnostics, disease monitoring, and therapeutic evaluation. This biosensor represents a significant advancement in the development of next-generation diagnostic tools.
研究领域:
生物传感技术
·
分子生物学
·
临床诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Mingyu Xia,Songsong Zhao,Zhiwei Sun,Yan Shi,Wenhai Lin,Zhiyuan Zhong,Fenghua Meng
Glioblastoma (GBM) is among the most aggressive brain tumors, presenting significant therapeutic challenges due to intrinsic and acquired resistance to treatment, alongside a highly immunosuppressive tumor microenvironment (TME). While temozolomide (TMZ) is the standard chemotherapeutic agent with the ability to penetrate the blood–brain barrier (BBB), its clinical efficacy is often limited. Here, we report a strategy employing Apolipoprotein E (ApoE) peptide-functionalized polymersomes loaded with small interfering RNA (siRNA) targeting signal transducer and activator of transcription 3 (Apstat3) to amplify the anti-GBM effects of TMZ and immunotherapy. Apstat3 demonstrated small, uniform particle sizes, stability in siRNA encapsulation, and effective downregulation of STAT3 and O⁶-methylguanine-DNA methyltransferase (MGMT) in GL261 cells, sensitizing these tumor cells to TMZ. The combinatorial approach not only significantly inhibited GBM cell proliferation, migration and invasion but also improved dendritic cells (DCs) maturation under TME-mimicking environment. In orthotopic GL261 mouse models, intravenous injection of Apstat3 co-administered with oral TMZ resulted in a twofold increase in median survival and reshaped the TME. Notably, combined treatment with anti-CTLA4 therapy tripled median survival to 64 days, achieving complete remission observed in 20 % of the mice. This siSTAT3 delivery strategy holds promise for enhancing GBM treatment outcomes.
研究领域:
肿瘤学
·
药剂学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Pan-Pan Guo,Xi-Bin Yang,Hong Yang,Cao Zhou,Gui-Yun Long,Dao-Chao Jin
β -N-Acetylglucosaminidases and/or β - N -acetylhexosaminidases (NAGs / Hexes) are crucial exonucleases, playing a crucial role in the insect molting process. SfHex3 and SfHex4 contain conserved catalytic domains of GH20 and GH20b, clustered into NAG2 and NAG1 group, respectively. SfHex3 and SfHex4 were mainly highly expressed in the 4th–5th instar nymphs, as well as in the integument and ovary. The expression level of SfHex3 gradually decreased in male and female adults, and SfHex4 on the first day of female was significantly higher than that on the first day of male. In addition, RNA interference (RNAi) results demonstrated that the downregulation of SfHex3 and SfHex4 expression in 5th-instar nymphs resulted in failed molting, and a high mortality. Furthermore, after RNAi with SfHex3 and SfHex4 , the transcript levels on key genes of the chitin metabolism pathway ( SfCHS1 , SfCHS1a , SfCHS1b , SfTRE1 , SfTRE2 , SfCht5 , and SfCht7 ) were significantly decreased compared to the control group. Meanwhile the expression levels of SfHex3 and SfHex4 were up-regulated after 6 h and 12 h of 20E treatment. And the transcription levels of SfHex3 and SfHex4 were significantly inhibited at nitenpyram LC 20 , LC 50 , and LC 90 after 96 h of treatment, in 3rd nymphs of Sogatella furcifera . In conclusion, SfHex3 and SfHex4 play important roles in the nymphal development of S. furcifera , contributing to the molting process from nymph to adult. This study not only enhances our understanding of the nitenpyram in pest control, but also provides a foundation for the development of new control strategies using RNAi to targeting SfHex3 and SfHex4 .
研究领域:
昆虫学
·
分子生物学
✓ 已验证
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Zhang Yue,Zhang Sufang,Chu Yadong,Zhang Qi,Zhou Renhui,Yu Di,Wang Shuang,Lyu Liting,Xu Guowang,Zhao Zongbao Kent
The basidiomycetous yeast Rhodosporidium toruloides (R. toruloides) is an excellent producer for neutral lipids, including triacylglycerols (TAG). Partially because genetic tools for this yeast were less developed, limited efforts were shown to explore its capacity for the production of higher-value lipids such as diacylglycerols (DAG). Here, four genes linked to the interconversion between DAG and TAG were manipulated to promote the production of DAG and free fatty acids (FFA). Among them, three TAG synthesis-related genes, DGA1, LRO1, and ARE1, were down-regulated successively via the RNA interference technology, and an endogenous TAG lipase encoded by TGL5 was fused with LDP1 and over-expressed to convert TAG into DAG and FFA. Results showed that those engineered R. toruloides strains grew normally under nutrient-rich conditions but notably slower than the parental strain NP11 in the lipid production stage. When cultivated in nitrogen-limited media, engineered strains were able to produce total lipids with improved contents of DAG and FFA by up to two-fold and three-fold, respectively. Further correlation analysis between lipid composition and cell density indicated that the formation of TAG correlated positively with cell growth; however, other lipids including DAG did negatively. This study offered valuable information and strains to engineer R. toruloides for advanced production of fatty acid derivatives.
研究领域:
微生物学
·
遗传学
·
代谢工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Insect Science
·
2025
·
IF 3
Delong Kong,Zitong Wang,Hui Guo,Tao Lin,Dingxin Jiang,Hualong Qiu,Tao Ma,Chunsheng Jia,Lufei Xie,Yongyue Lu,Feng Liu,Changlu Wang,Desen Wang
Carbon dioxide (CO 2 ) plays a crucial role in the host search/localization process of bed bugs, Cimex lectularius L., and Cimex hemipterus (F.). Current research on the CO 2 perception by bed bugs mainly focuses on their behavioral responses, and the molecular mechanisms are still unclear. In addition, existing research has mainly been conducted on C. lectularius with very little research on C. hemipterus . In this study, we investigated the behavioral response of C. hemipterus to CO 2 and analyzed the role of antennae in the CO 2 sensing process. Then, potential CO 2 receptor genes were screened through antennal RNA sequencing and tissue-specific expression profiling analysis. Finally, the function of CO 2 receptors was further validated using RNA interference. Results indicated that increasing the CO 2 concentration in the air not only stimulated the activity of C. hemipterus , but also presented a directional attraction effect on them. CO 2 , with a concentration of 0.06%–1.8%, had a significant attraction effect on C. hemipterus . Removal of the antennae led to the loss of bed bugs’ preference for CO 2 . Four candidate CO 2 receptor genes ( ChGR1 , ChGR2 , ChGR3 , and ChGR4 ) were identified through transcriptomic analysis of the bed bug antennae, and 3 of them ( ChGR2 , ChGR3 , and ChGR4 ) were highly expressed in the antennae. Silencing ChGR2 or ChGR4 individually led to a significant reduction or even loss of CO 2 sensing ability in C. hemipterus . In conclusion, CO 2 can induce the host searching behavior of C. hemipterus ; moreover, ChGR2 and ChGR4 are crucial for detecting CO 2 in C. hemipterus .
研究领域:
昆虫学
·
分子生物学
·
生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Physiology
·
2022
·
IF 4.755
Hafeez Muhammad,Li Xiaowei,Ullah Farman,Zhang Zhijun,Zhang Jinming,Huang Jun,Fernández-Grandon G. Mandela,Khan Muhammad Musa,Siddiqui Junaid Ali,Chen Limin,Ren Xiao Yun,Zhou Shuxing,Lou Yonggen,Lu Yaobin
The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), is a pest of many important crops globally. Effective control is challenging, with the pest exhibiting resistance to different synthetic pesticides across various groups. However, the mechanisms employed by resistant insects for overexpression of relevant detoxification genes remain unclear. The activity of detoxification enzymes was investigated in this study. Additionally, using RNA interference (RNAi), a functional analysis was completed of two P450s genes in an indoxacarb resistant population of fall armyworms. Elevated resistance levels (resistance ratio = 31.37-fold) in indoxacarb-selected populations of FAW were observed after 14 generations. The qRT-PCR showed higher expression of two cytochrome P450 genes, CYP321A7 and CYP6AE43, in this selected population compared to the control population. RNAi was applied to knock down the P450 dsCYP321A7 and dsCYP6AE43 genes in the FAW larvae. Droplet feeding of the dsRNAs (CYP321A7 and CYP6AE43) via an artificial diet significantly increased mortality rates in the indoxacarb treated population. A shorter larval developmental time of FAW was detected in all dsRNAs-fed larvae. Correspondingly, larval mass was reduced by dsRNAs in indoxacarb resistant populations of fall armyworm. Larval feeding assays demonstrate that dsRNAs targeting, specifically of CYP321A7 and CYP6AE43 enzymes, could be a beneficial technique in the management of indoxacarb resistant populations. Further study on the potential use of dsRNA and its application should be conducted in efforts to counter the development of resistance in FAW against various insecticides in the field.
研究领域:
昆虫学
·
分子生物学
·
害虫管理
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）