ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yusi Li,Fang Yang,Shunmei Li,Ruo Yuan,Yun Xiang
Proteins play vital roles in regulating a series of living activities and can be potentially considered to be useful indicators for diagnosing various diseases. Here, a target-triggered transcription cascade amplification strategy with the use of the fluorescence switch-on RNA aptamer is constructed for label-free and sensitive platelet-derived growth factor-BB (PDGF-BB) protein biomarker detection. The target molecule of PDGF-BB binds a rationally designed multifunctional hairpin probe to switch its structure and to expose a primer binding region. The primer sequence further binds such a region to initiate the target recycling and lighting-up RNA aptamer transcription cascade cycles to yield tremendous RNA aptamers. And, the fluorescence of the organic dye is substantially enhanced upon binding to these RNA aptamers for realizing highly sensitive and label-free PDGF-BB detection with the detection limit being lowered to 0.8 pM. Moreover, the developed strategy has superior selectivity and exhibits a promising potential to detect PDGF-BB spiked into serums with buffer dilution, which makes this method an attractive sensing system for detecting other biomarkers at trace levels.
研究领域:
生物化学
·
分子生物学
·
医学诊断
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DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Tiantian Chen,Qian Xiang,Xuemei Sang,Daxiu Li,Wenjiao zhou,Fengfeng Xu
Malaria remains a significant threat to global health, especially with the emergence of drug-resistant strains. Therefore, precise monitoring of antimalarial drug levels is crucial for assessing and managing drug resistance. Here, we report a multiplex aptasensor that enables simultaneous fluorescence detection of two antimalarial drugs (MQ and PQ) via split dsT7 promoter-mediated transcription of two-color light-up RNA aptamers. The detection mechanism relies on specific binding of target antimalarial drugs (MQ/PQ) to their aptamers, each triggering formation of intact dsT7 promoter to induce amplified transcription of its cognate RNA aptamer. The generated RNA aptamers bind their corresponding organic dyes (TO1-Biotin and MG) with high affinity, yielding 7.7-/25-fold fluorescence enhancement to enable ultrasensitive detection of MQ and PQ with limits of 0.71 ng/mL and 0.069 ng/mL, respectively. The method also exhibits high selectivity and low background signal. Furthermore, this label-free system achieves multiplexed detection of antimalarial drugs in 10-fold diluted human serum (recovery: 98.67–106.84 % for PQ and 99.89–106.19 % for MQ), demonstrating its real utility. This label-free system is adaptable to other biomarkers through aptamer replacement.
研究领域:
生物化学
·
分子生物学
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2016
·
IF 1.088
Ting Chen,Lina Tang,Fan Yang,Qiang Zhao,Xin Jin,Yong Ning,Guo-Jun Zhang
A novel electrochemical aptasensor is reported for the label-free determination of dopamine by electrochemically reduced graphene oxide and a gold nanocomposite with a ribonucleic acid (RNA) aptamer. The nanocomposite was fabricated by layer-to-layer electrochemical deposition. The aptamer was immobilized on the surface of the gold nanoparticles through the formation of thiol–gold bonds. Dopamine was determined through specific interaction with the immobilized aptamer. The redox activity of the analyte enabled direct electrochemical analysis. The conductivity and structure of the nanocomposite were characterized by cyclic voltammetry and scanning electron microscopy. In contrast to bare or reduced graphene oxide-modified electrodes, the nanocomposite-modified electrode significantly enhanced the current response. The electrochemical aptasensor provided a detection limit of 0.13 µM with a linear dynamic range from 0.5 to 20 µM. In addition, the sensor was shown to provide high selectivity and satisfactory stability for the determination of dopamine in the presence of analogs and in human serum.
研究领域:
分析化学
·
生物医学工程
·
材料科学
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产品关联
DOI
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 47
·5629-5633
·
IF 2.073
Hui Guo,Mengyue Fan,Zengjin Li,Wei Tang,Xinrui Duan
RNAs play critical roles in most cellular and developmental processes. The RNA in vitro synthesis quantification method with low background without requiring translation is highly desirable. In this study, we have described a ratiometric RNA aptamer–fluorophore complex, Spinach2/DFHBI–CM, for quantifying the in vitro synthesis of RNA. DFHBI–CM exhibits bright fluorescence upon excitation at 337 nm with or without RNA, and displays the characteristic emission band of triazolyl-coumarin centered at 420 nm. When DFHBI–CM is combined with RNA aptamer Spinach2 to generate Spinach2/DFHBI–CM fluorescence complexes, under the excitation wavelength of 447 nm, the fluorescence intensity at 502 nm is significantly enhanced. By comparing the emission intensity at 420 nm and 502 nm, ratiometric fluorescence measurement is achieved. Based on this principle, DFHBI–CM was applied as a fluorophore in RNA aptamer–fluorophore complex for ratiometrically detecting and quantifying RNA in vitro synthesis. Our design has the following advantages: (1) ratiometric fluorescence can minimize the environmental inferring factors and thus, more accurate and effective detection can be achieved; (2) compared with run-off transcription detection of RNA, this ratiometric fluorescent probe reports immediately the quantity of RNA synthesized in vitro.
研究领域:
分子生物学
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生物化学
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合成生物学
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产品关联
DOI
高影响因子
Food Analytical Methods
·
2018
·Vol. 11
·期 6
·1668-1676
·
IF 2.245
Jia Jie,Yan Shang,Lai Xiaoxia,Xu Yongzhen,Liu Tong,Xiang Yuhong
A facile colorimetric aptasensor based on unmodified gold nanoparticles (AuNPs) for rapid, highly sensitive and selective detection of malachite green (MG), one of triphenylmethane dyes and antibiotics, was successfully developed. Malachite green RNA aptamer having the binding ability with high affinity and specificity would be the ideal recognition receptor for MG. RNA aptamer can adsorb on the AuNPs and protect AuNPs against salt-induced aggregation. When malachite green were added in a solution, aggregation of AuNPs was specifically induced by desorption of aptamer from the surface of gold nanoparticles as the formation of aptamer-target complex. Therefore, the salt could trigger AuNP aggregate and the solution color changed from red to blue. The solution colors and absorbance were related with the concentrations of MG, and a well linear relationship in the wide range was obtained. The detection limit of MG was down to 15.95 nM. This colorimetric method was better than other traditional methods in terms of its reduced detection time, simple signal generation, high selectivity, lower detection limit, and observation with the naked eye. The proposed one-step, label-free method was successfully used to determine MG in actual fish samples. The high recoveries of MG in fish sample detection were satisfied.
研究领域:
分析化学
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环境科学
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纳米技术
·
食品安全
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Chengyu Zhang,Weifan Dang,Jingjing Zhang,Cong Wang,Peng Zhong,Zhaoxin Wang,Yufan Yang,Yuefei Wang,Xiaohui Yan
The abnormal uric acid (UA) level in urine can serve as warning signals of many diseases, such as gout and metabolic cardiovascular diseases. The current methods for detecting UA face limitations of instrument dependence and the requirement for non-invasiveness, making it challenging to fulfill the need for home-based application. In this study, we designed an aptasensor that combined UA-specific transcriptional regulation and a fluorescent RNA aptamer for convenient urinary UA testing. The concentration of UA can be translated into the intensity of fluorescent signals. The aptasensor showed higher sensitivity and more robust anti-interference performance. UA levels in the urine of different volunteers could be accurately tested using this method. In addition, a paper-based aptasensor for UA self-testing was manufactured, in which the urinary UA levels could be determined using a smartphone-based colorimetric approach. This work not only demonstrates a new approach for the design of disease-associated aptasensor, but also offers promising ideas for home-based detection of UA.
研究领域:
分析化学
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生物传感器
·
即时检测
·
分子诊断
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 105
·60987-60994
·
IF 3.708
Hongzhi Wang,Yu Wang,Su Liu,Jinghua Yu,Wei Xu,Yuna Guo,Jiadong Huang
An RNA aptamer-based electrochemical biosensing strategy has been developed for sensitive and selective detection of malachite green (MG). This biosensor is fabricated by the self-assembly of a thiolated MG aptamer (MGA) on AuNPs/graphene–chitosan nanocomposite modified glass carbon electrode. In addition, a short alkanethiol is further assembled on the AuNPs surface to generate uniform packing and reduce nonspecific adsorption. When the modified electrode is incubated in the presence of MG, MGA combines specifically with MG, which causes the horseradish peroxidase (HRP)-labelled MG antibody close to the electrode surfaces. As a result, MG detection is realized by outputting a redox current from electro-reduction of the hydrogen peroxide reaction catalyzed by HRP. Differential pulse voltammetry (DPV) is performed to record the signal responses. The results reveal the biosensor displays a very low detection limit as low as 16.3 pg mL−1 and a wide linear range from 1 × 10−4 to 10 μg mL−1 of MG. Hence, this proposed RNA aptamer-based electrochemical strategy may offer a simple, rapid, cost-effective, highly selective and sensitive method for the quantification of MG.
研究领域:
分析化学
·
环境科学
·
生物传感器
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Junchun Wang,Jinjin Guo,Junjun Zhang,Wenjuan Zhang,Yuzhong Zhang
C-reactive protein (CRP) is a widely accepted biomarker of cardiovascular disease and inflammation. In this study, a RNA aptamer-based electrochemical sandwich type aptasensor for CRP detection was described using the functionalized silica microspheres as immunoprobes. Silica microspheres (Si MSs), which have good monodispersity and uniform shape, were firstly synthesized. The silica microspheres functionlized with gold nanoparticles (Au NPs) provided large surface area for immobilizing signal molecules (Zinc ions, Zn 2+ ) and antibodies (Ab). RNA aptamers, which were specific recognized to CRP, were assembled on the surface of Au NPs modified electrode via gold-sulfur affinity. In the presence of CRP, a sandwich structure of aptamer-CRP-immunoprobe was formed. Square wave voltammetry (SWV) was employed to record the sensing signal, and a clearly reductive peak corresponding to Zn 2+ at about −1.16 V (vs. SCE) was obtained. Under optimal conditions, the aptasensor showed wide linear range (0.005 ng mL −1 to 125 ng mL −1 ) and low detection limit (0.0017 ng mL −1 at a signal-to-noise ratio of 3). Some possible interfering substance was also investigated, and the results obtained showed that the aptasensor possessed good selectivity . When the aptasensor was applied to real serum samples analysis, the satisfied results were obtained, indicating that the aptasensor possessed potential real application ability.
研究领域:
生物传感器
·
电化学
·
分析化学
·
生物工程
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DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2020
·
IF 6.215
Qingqing Zhang,Tingting Hao,Dandan Hu,Zhiyong Guo,Sui Wang,Yufang Hu
A new RNA aptamer-involved electrochemiluminescence (ECL) strategy for sensitive detection of histone acetyltransferase (HAT p300) is designed well based on coenzyme A (CoA)-initiated competitive response and advanced DNA nano-biotechnology. For the first time, this interesting analytical process is comprised of significant histone acetylation catalyzed by HAT p300, competitive replacement triggered by the specific binding of CoA and its RNA aptamer , stable scaffold guided by DNA nano-prism and typical amplification managed by hybrid chain reaction (HCR). Upon recognition of the RNA aptamer to CoA generated in acetylation, auxiliary DNA (A-DNA) which can be captured by three hanging arms of the fabricated DNA nano-prism immobilized onto Au electrode surface is released from the aptamer/A-DNA complex. Moreover, A-DNA can also open skillfully one hairpin DNA (H1), then turning into HCR process by alternating hybridization with the other hairpin DNA (H2). Abundant Ru(phen) 3 2+ molecules which have a strong interaction with dsDNA can result in a significant output. This proposed platform exhibits excellent specificity and sensitivity toward HAT p300 with a detection limit of 0.001 nM and takes on outstanding practicality and superiority, which presents a great potential for HAT p300 analysis in biomedical sciences.
研究领域:
分析化学
·
生物化学
·
分子生物学
·
生物医学科学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 5
·1-8
·
IF 5.479
Zhao Chen,Hong Cheng-yi,Lin Zheng-zhong,Chen Xiao-mei,Huang Zhi-Yong
A specific and sensitive colorimetric aptasensor is described for the determination of Malachite Green (MG). It is exploiting the inhibition of the peroxidase-like activity of gold nanoparticles (AuNPs). The AuNPs act as enzyme mimics that catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 to yield a dark blue solution. The catalytic activity is inhibited by hexadecyl trimethyl ammonium ion, specifically by cetyltrimethylammonium bromide (CTAB), which causes the aggregation of AuNPs. If a (negatively charged) RNA-aptamer against MG is added, it binds to the positively charged CTAB and prevents aggregation. This enhances the enzyme mimicking activity of the AuNPs and leads to the formation of a dark blue solution. However, in the presence of MG, the aptamer binds to MG, and leads to the aggregation of AuNPs again. The aggregated AuNPs possess a light blue color. A colorimetric method (best performed at 650 nm) was work out that can detect MG in a concentration range from 10 to 500 nmol L −1 . The detection limit based on 3σ/k criterion is 1.8 nmol L −1 . The assay is highly specific and accurate. Recoveries from spiked real samples (aquaculture water) ranged from 80% to 120%.
Based on the inhibition of cetyltrimethyal ammonium ion and the enhancement of RNA-aptamer, the differences of the peroxidase-like activities of AuNPs can be greatly enlarged with and without MG, by which a colorimetric aptasensor can be constructed for the detection of Malachite Green (MG).
研究领域:
分析化学
·
纳米技术
·
环境科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）