TALANTA
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2018
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IF 4.244
Kailun Deng,Lei Wang,Qi Xia,Ruiyuan Liu,Jinqing Qu
Nucleus imaging is of great importance for understanding cellular processes of genetic expression, proliferation and growth, etc. Although many nucleic-acid selective dyes for nucleus staining are available, few of them meet multiple standards. Herein, we report a cationic fluorescence dye FTI that possesses visible light excitation (436 nm), orange emission (571 nm) and a large Stokes shift (~135 nm) for nucleic-acid staining. FTI displays an obvious and sensitive fluorescent response to DNA in vitro with a 6.4-fold quantum yield increasing. Co-staining and nucleic acid digest experiments in live cells demonstrate that FTI exhibits an unexpected selectivity for the nucleolus of the cells due to the stronger affinity to RNA than DNA. Because of good photostability and low cytotoxicity, FTI can accomplish a promising stain for DNA recognition in vitro and nucleolus-specific imaging in cancer cells.
研究领域:
细胞生物学
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分子生物学
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生物成像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2018
·
IF 8.097
Hengchang Ma,Yanfang Qin,Zengming Yang,Manyi Yang,Yucheng Ma,Pei Yin,Yuan Yang,Tao Wang,Ziqiang Lei,Xiaoqiang Yao
Fluorescence-tunable materials are becoming increasingly attractive because of their potential applications in optics, electronics, and biomedical technology. Herein, a multicolor molecular pixel system is realized using a simple copolymerization method. Bleeding of two complementary colors from blue and yellow fluorescence segments reproduced serious multicolor fluorescence materials. Interestingly, the emission colors of the polymers can be fine-tuned in the solid state, solution phase, and in hydrogel state. More importantly, the positive fluorescent polymers exhibited cell-membrane permeable ability and were found to accumulate on the cell nucleus, exhibiting remarkable selectivity to give bright fluorescence. The DNA/RNA selectivity experiments in vitro and in vivo verified that [tris(4-(pyridin-4-yl)phenyl)amine]-[1,8-dibromooctane] has prominent selectivity to DNA over RNA inside cells.
研究领域:
生物医学工程
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材料科学
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分子生物学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
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IF 7.46
Ning Xu,Jianjun Du,Qichao Yao,Haoying Ge,Chao Shi,Feng Xu,Liman Xian,Jiangli Fan,Xiaojun Peng
Targeting capability is one of the most important properties of both imaging and therapeutic agents. The discovery of “structure-inherent targeting” (SIT) phenomena has benefitted the tailored design of fluorescent probes and photodynamic photosensitizers. Herein, SIT was combined with the inheritance of epigenetic characteristics from parent carbon sources to synthesize nucleic acid-targeted carbon dots (CDs) from quinoline derivatives, namely, 1-CDs, Cl-CDs, and I-CDs via a bottom-up strategy. These CDs exhibited RNA-selective fluorescence imaging, as confirmed using cell digestion, SYTO® RNASelect™ costaining, and nucleic acid titration. Furthermore, owing to iodine doping, I-CDs exhibited excellent PDT performance for killing cancer cells in vitro. Importantly, I-CDs could actively target and become enriched at tumor sites using folic acid-functionalized liposomes as tumor-homing vehicles, thus effectively inhibiting tumor growth in vivo. By screening the SIT carbon source, photodynamic CDs can provide a new platform for next-generation nucleic acid-targeting photodynamic therapy.
研究领域:
纳米技术
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生物医学工程
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癌症研究
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
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IF 4.821
Yanhong Liu,Ping Chen,Kangkang Yu,Zhening Wu,Na Wang,Xiaoqi Yu
Heteroatoms doped carbon dots (CDs) have attracted extensive attention for their superior optical properties, facile preparation and multifunctional applications in environmental detection, cell imaging and gene delivery. Herein, Nitrogen and sulfur co-doped carbon dots (N,S-CDs) were synthesized directly from methyl cellulose (MC), l -cysteine ( l -cys) and ethylenediamine (EDA), showing excitation-dependent emission properties and relative high quantum yield (QY) of 12.3%. The obtained N,S-CDs are responsive to pH and temperature, suggesting pH- and temperature-dependent fluorescent features. Moreover, N,S-CDs are utilized to image and exhibit selective location inside the nucleus region both in living and fixed cells. Relative mechanisms have been explored by ribonuclease digestion testing and co-staining analysis with commercial nucleic acid dye. N,S-CDs show the specifical distribution in RNA-rich regions and positive response to RNA in cells. In brief, the developed N,S-CDs possess multi-faced application potency in pH sensing, temperature monitoring and RNA-selective imaging in cells.
研究领域:
材料科学
·
分析化学
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生物化学
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细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）