Real-time and high-efficient detection of food additives is of great significance for global public health. As a popular biosensor, colorimetric detection has been widely applied in various fields, including the food industry. However, owing to the complex structure, weak ROS-catalytic activities and unclear catalytic mechanism, recently reported biocatalysts as biosensors still face major challenges in achieving high-efficient and multimodal aspects. Here, the novel design of high-efficient, robust, multimodal, and Os-based active centers on the oxygen vacancies (O v )-containing Cu 2 Cl(OH) 3 substrates (Os@TBCC-O v ) to serve as ROS-catalytic biosensors to colorimetric detection of food additives is reported. Benefiting from the highly active and excellent electronic structure of Os-single-atom sites, the synthesized Os@TBCC-O v biocatalysts exhibit superior POD-mimetic activities, thus facilitating the exceptional and exclusive detecting activities. Moreover, colorimetric studies found that the POD-mimetic biosensor of Os@TBCC-O v can provide high-efficient, wide-range and visual detection abilities of food additives, including H 2 O 2 , L-Cysteine and glucose, which is much higher efficient than that of previously reported biosensors. Meanwhile, the results of practical application further demonstrate the feasibility of Os@TBCC-O v -based biosensors in detection of food additives. It is suggested that this design will offer a novel pathway for high-efficient colorimetric detection of POD-mimetic biocatalysts in food industry and other related fields.
Pathogenic drug-resistant bacteria represent a threat to human health, for instance, the methicillin-resistant Staphylococcus aureus (MRSA). There is an ever-growing need to develop non-antibiotic strategies to fight bacteria without triggering drug resistance. Here, we design a hedgehog artificial macrophage with atomic-catalytic centers to combat MRSA by mimicking the “capture and killing” process of macrophages. The experimental studies and theoretical calculations reveal that the synthesized materials can efficiently capture and kill MRSA by the hedgehog topography and substantial generation of •O 2 − and HClO with its Fe 2 N 6 O catalytic centers. The synthesized artificial macrophage exhibits a low minimal inhibition concentration (8 μg/mL Fe-Art M with H 2 O 2 (100 μM)) to combat MRSA and rapidly promote the healing of bacteria-infected wounds on rabbit skin. We suggest that the application of this hedgehog artificial macrophage with “capture and killing” capability and high ROS-catalytic activity will open up a promising pathway to develop antibacterial materials for bionic and non-antibiotic disinfection strategies.
危险品化学品经营许可证（带存储）