Reactive oxygen species (ROS) play a fundamental role in antibacterial therapeutic strategies. However, excessive ROS generation can disrupt redox homeostasis and induce a severe inflammatory response, causing immense stress on surrounding cells and tissues. Herein, an intelligent nanoheterojunction established with Prussian blue (PB) and manganese polyphthalocyanine (MnPPc), PB@MnPPc (PM), was established to achieve sequential antibacterial and anti-inflammatory functions via controllable ROS regulation. With spatiotemporal control of near-infrared (NIR, 808 nm) light, PM demonstrates the potential for on-demand generation and scavenging of ROS. When exposed to NIR irradiation, PM exhibits prominent bactericidal abilities, which result from its augmented singlet oxygen generation capacity and photothermal effects. These advantages originate from the narrow band gap as well as the enhanced electron-hole separation and transfer efficiency enabled by its unique heterostructure. Upon cessation of NIR irradiation, PM efficiently achieves ROS elimination and inflammation alleviation through its superior superoxide dismutase-like and catalase-like activities. In vivo studies in a mouse Staphylococcus aureus -infected skin wound model further confirmed the superior therapeutic effects of PM, which effectively eliminated bacteria, relieved inflammation, promoted collagen deposition, and angiogenesis. This work provides an attractive therapeutic modality for equipping nanoheterojunctions with antibacterial and anti-inflammatory properties through programmed ROS regulation.
Wenying Zhang,Meifang Wang,Bin Liu,Meng Yuan,Zhuang Yang,Jia Tan,Ping'an Ma,Jun Lin
The tumor thermotolerance owing to the overexpression of heat shock proteins (HSPs) is the critical challenge in practical application of mild-temperature photothermal therapy (MPTT). Reactive oxygen species (ROS) can not only serve as a powerful weapon to kill cancer cells, but also a regulator to inhibit HSPs, thus improving the thermal sensitivity of tumors. Herein, a multi-model ROS regulation nano-platform based on TCLP nanocomposites consisting of polyacrylic acid encapsulated, chloroperoxidase (CPO) and β-Lapachone (β-Lap) loaded black mesoporous titanium (H-TiO 2 ) has been constructed for the ROS-enhanced MPTT. In this nano-platform, the H-TiO 2 acts as the NIR-Ⅱ light triggered photothermal agent and photosensitizer to contribute to deep tissue MPTT and PDT, the β-Lap acts as a H 2 O 2 amplifier to improve the 1 O 2 production of CPO. The abundant ROS from TCLP NPs can limit the activity as well as the expression of HSPs, ultimately improving the antitumor efficacy of MPTT. Finally, excellent synergistic tumor inhibition based on TCLP NPs has been reached both in vitro and in vivo. This multi- model ROS enhanced MPTT nano-platform with low toxic side effects offers a promising strategy and enlightenments the clinical application of MPTT.
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